The Influence of Varying Wavelengths of LED Light on the Development, Physiology Response, and Metabolism Activities of Micropropagated Dendrobium Hybrid ‘Shuijing’ Plantlets

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The results presented in the manuscript are very valuable and interesting. The literature review is not very extensive but current and on topic. The work contains all the necessary elements of scientific research and after minor editorial corrections, in my opinion the results of the work can be published. In future research, I encourage the Authors to be interested in another light wavelengths, besides of red and blue, which are already quite well investigated compared to other light spectra (green, orange, yellow etc.) and its impact on plants growth and development.

 

I have a few comments:

1)     After each quotation there is no space before the bracket – is that correct?

 

2)     Supplementary Material: some corrections of spacing in the text is needed. Also the labeling in the tables in the first cell should be in English, not Japanese. Needed changes marked in the supplementary material MS Word file in “Track Changes” tool. Also, in my opinion (as most publishers), “in vitro” we should no longer write in italics – this term is common – change it please in “supplementary material” text.

Comments for author File: Comments.pdf

Author Response

Comments 1:  After each quotation there is no space before the bracket – is that correct?

Response 1: Thank you very much for your suggestion, we have modified the quotation in the text, and spaced before each quotation.

Comments 2:  Supplementary Material: some corrections of spacing in the text is needed. Also the labeling in the tables in the first cell should be in English, not Japanese. Needed changes marked in the supplementary material MS Word file in “Track Changes” tool. Also, in my opinion (as most publishers), “in vitro” we should no longer write in italics – this term is common – change it please in “supplementary material” text.

Response 2: Thank you very much for your suggestion. We have changed the label of the first cell in the table to English and canceled the italic format of ' in vitro '.

 

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

Influence of Varying Wavelengths of LED Light on the Development, Physiology Response, and Metabolism Activities of Micropropagated Dendrobium Hybrid ‘Shuijing’ Plantlets

What difference will there be with the other varieties of Dendrobium?

Keywords

Skip words contained in the title

Abstract

Line 17 Write in the third person.

Line 24 Write in the third person.

Lines 24-33 did all this happen in red and blue LED lights?

In general, well-written summary, presenting detailed information about the experiment and notable results. However, information on many correlations is given and it is not specified in which LED Light condition these results were obtained.

 

 1.Introduction

Line 44 mention that it means LED.

Lines 56-73 Accommodate morphological and physiological responses. It could also be by LED color.

Lines 74-76 are part of the discussion.

Line 84-86 that statement is wrong a simple search for Dendrobium and LEDS.

 

1.     Guo, Y., Zhong, Y., Mo, L., Zhang, W., Chen, Y., Wang, Y. C., ... & Meng, X. (2023). Different combinations of red and blue LED light affect the growth, physiology metabolism and photosynthesis of in vitro-cultured Dendrobium nobile ‘Zixia’. Horticulture, Environment, and Biotechnology, 64(3), 393-407.

2.     Ahmad, H. I., Zhang, J., Luo, F., Iqbal, O., & Wang, Y. (2024). Evaluating the impact of various LED light spectrums on Dendrobium officinale tissue culture seedlings. Sarhad Journal of Agriculture, 40(2), 615-624.

3.     Yeow, L. C., Chew, B. L., & Sreeramanan, S. (2020). Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects. Biotechnology Reports, 27, e00497.

4.     Li, X., Yang, K., Ma, J., Wu, S., Xu, E., Liu, Y., ... & Fan, Y. (2023). Transcriptome analysis reveals different red and blue light duration promote growth and main medicinal ingredients of Dendrobium nobile Lindl. Industrial Crops and Products, 204, 117347.

5.     Mehbub, H., Shimasaki, K., & Mehraj, H. (2022). Low concentration of anti-auxin and anti-fungal agent accelerates the PLB regeneration of Dendrobium okinawense under green LED. Plants, 11(8), 1082.

6.     Etc.

 

Line 88 go to third person.

In general, introduction should be improved.

 

Materials and methods

Line 103 MS 40 mg · L – 1? Only 40 mg per liter? Generally 4.0 -4.5 g L-1 are used

What happened to the vitamins?

Line 104 and the seedlings remained rootless? If you add that XXX cm long shoots were used, it would be understood that they do not have roots.

Line 106 the relative humidity was 40 % - 50 %? When using covered jars (semi-solid) the humidity is maintained between 98-100%.

Line 114, the brand of LED lights used should be mentioned.

Why didn't 80 blue + 20 red exist? And 70 blue + 30 red?

Figure 1 contains no description for a,b,c, etc.

Line 135 Why 50 days of cultivation? Why only 10 plants? How were these plants selected? Random?

Line 137 number, length, area? Clarify.

Lines 140-166 must indicate how many samples were taken per treatment and how many measurements.

3. Results

Lines 180-190 describe results according to the order of the table (by column).

Lines 190-194 in which table is shown in statistical analysis of this experiment?

Table 4 would be more demonstrative if it were a graph.

4. Discussion

Lines 365-379 this is part of the introduction

Lines 384-395 Do not repeat what is stated in the results, you must describe variable by variable and discuss similarities or contrasts with other studies.

 

In general, the discussion should be improved and have a logical order, as described in results.

 

Conclusion

Line 524 plant architecture?

It must be adapted to what the demdrobium cultivation industry requires, more sugars, more plant height, leaves, etc.

In general, this study is relevant. However, it is not a new topic. Major modifications are required to be accepted.

Author Response

Comments 1:” What difference will there be with the other varieties of Dendrobium?

Response 1:Thank you for pointing this out.Dendrobium can be categorized into two distinct groups: ornamental and medicinal. Dendrobium ‘Shuijing’ discussed in this paper possesses both aesthetic and therapeutic value. There have been numerous studies conducted on the impact of LED lighting on Dendrobium. However, due to the species-specific and varietal-dependent nature of light quality, there is currently a lack of research articles specifically addressing the effects on Dendrobium‘Shuijing’, which holds significant implications for production. If any further improvements are necessary, we will make revisions immediately.

Keywords

Comments 2: Skip words contained in the title,42-43

Response 2: Agreed. Therefore, we have modified keywords according to your suggestion.

Abstract

Comments 3: Line 17 Write in the third person.

Response 3: Thank you for pointing this out, we have changed the sentence's from first person to third person.15

Comments 4: Abstract, Line 24 Write in the third person.

Response 4: Thank you for pointing this out, thanks to your suggestion, we have changed the sentence's from first person to third person.22

Comments 5: Abstract,Lines 24-33 did all this happen in red and blue LED lights?

Response 5:Thank you for pointing this out, we have modified it according to your suggestion.

Lines 24-33 all this happen in the combination of 7 R : 3 B. There is no explicit expression due to our negligence. We have modified it according to your suggestion.

Comments 6: In general, well-written summary, presenting detailed information about the experiment and notable suggestion. However, information on many correlations is given and it is not specified in which LED Light condition these suggestion were obtained.

Response 6:Agreed. Therefore, the abstract has been revised to enhance the clarity of the experimental suggestion' representation. Thank you for your valuable suggestion.

1. Introduction

Comments 7:Line 44 mention that it means LED.

Responses 7: Thank you very much for your comments. According to the problem, our current understanding is as follows: The spectrum of sunlight varies from 300 to 2600 nm, and the wavelengths that directly affect plant photosynthesis are between 400 and 700 nm, in which blue (B) light (400-450 nm) and red (R) light (600-700 nm) play important roles. Line 42of this article mention that it means LED. However, we would greatly appreciate any feedback you may have to offer if the answers do not meet your requirements.

Comments 8:Lines 56-73 Accommodate morphological and physiological responses. It could also be by LED color.

Response 8: Thank you very much for your comments. We agree with you that LED light can affect the morphological and physiological responses of plants in line 56-73. However, we would greatly appreciate any feedback you may have to offer if the answers do not meet your requirements.

Comments 9: Lines 74-76 are part of the discussion.

Response 9: The issue stems from our negligence. We have revised relevant contents in accordance with your suggestion.77-78

Comments 10: Line 84-86 that statement is wrong a simple search for Dendrobium and LEDS.

Response 10:  Agreed. Therefore, we have modified it according to your suggestion.94-95

Materials and methods

Comments 11:Line 103 MS 40 mg · L – 1? Only 40 mg per liter? Generally 4.0 -4.5 g L-1 are used

Response 11: This is our negligence, we modified the wrong expression according to our experimental content.107

Comments 12:What happened to the vitamins?

Response 12:Thank you very much for your reply. The medium component utilized isspecifically tailored for Dendrobium hybrid‘Shuijing’plantlets, while the essential vitamins such as niacin,thiamine hydrochloride, and pyridoxine hydrochloride have been added to the mother liquor in MS when it was prepared. Therefore, no other vitamins are added separately except banana powder.

Comments 13:Line 104 and the seedlings remained rootless? If you add that XXX cm long shoots were used, it would be understood that they do not have roots.

Response 13：Thank you very much for your comments. We explained the rootless seedlings in detail. 108

Comments 14:  Line 106 the relative humidity was 40 % - 50 %? When using covered jars (semi-solid) the humidity is maintained between 98-100%

Response 14:Agreed. Therefore, we modified the wrong expression according to our experimental content，corrected to the correct ambient humidity. 111

Comments 15: Line 114, the brand of LED lights used should be mentioned.

Response 15:Agreed. We have added the brand of LED lights used in line 120 according to your suggestion.

Comments 16:Why didn't 80 blue + 20 red exist? And 70 blue + 30 red?

Response 16: Thank you for your comment, 80 blue + 20 red and 70 blue + 30 red have been designed in this article in line 122 However, we would greatly appreciate any feedback you may have to offer if the answers do not meet your requirements.

Comments 17:Figure 1 contains no description for a,b,c, etc.

Response 17:Agreed, this is our negligence and we have added the description for a,b,c, d,e,f in figure 1. 141

Comments 18: Line 135 Why 50 days of cultivation? Why only 10 plants? How were these plants selected? Random?

Response 18:  Thank you very much for your comment, this description in line 135 was our negligence. The experimental materials was taken after 45 days of culture, and we have modified it. There were 50 bottles of material for each light quality treatment, and 10 bottles were selected from each treatment randomly for external morphological measurements.144-146

Comments 19:  Line 137 number, length, area? Clarify.

Response 19:Agreed. Therefore, it referred to the number of roots and leaves in line 137. We have clarified it accroding to your suggestion.146

Comments 20: Lines 140-166 must indicate how many samples were taken per treatment and how many measurements.

Response 20: Agreed. Therefore, we have indicated what the numbers of the samples were taken per treatment and how many measurements in line 140-166 and modified them in article according to your suggestion.144-177

2. Result

Comments 21: Lines 180-190 describe results according to the order of the table (by column).

Response 21: Agreed, we have modified the table according to your suggestions, in the order in which the suggestion are described.

Comment 22: Lines 190-194 in which table is shown in statistical analysis of this experiment?

Response 22: Thank you very much for your comment, figure 2 is the statistical analysis suggestion of this experiment. We have already labeled it in article according to your suggestion.

Comment 23: Table 4 would be more demonstrative if it were a graph.

Response 23: Agreed. Therefore, Thank you very much for your comments. We have substituted the

graph for table 4 according to your suggestion.

3. Discussion

Comment 24: Lines 365-379 this is part of the introduction

Response 24: Agreed. Therefore,thank you very much for your comments. We have modified it according to your suggestion. 386-401.

Comment 25: Lines 384-395 Do not repeat what is stated in the results, you must describe variable by variable and discuss similarities or contrasts with other studies

Response 25: Agreed. Therefore, we have modified the contents of this part according to your suggestion.

Comment 26: In general, the discussion should be improved and have a logical order, as described in results.

Response 26: Agreed. Thank you very much for your comments. We have modified the contents of discussion according to your suggestion.

4. Conclusion

Comment 27: Line 524 plant architecture?

Response 27: Thank you very much for your comments, the term ‘architecturewas’ was an inaccurate statement in line 563. We have replaced architecture with photomorphogenesis.

Comment 28: It must be adapted to what the demdrobium cultivation industry requires, more sugars, more plant height, leaves, etc.

Response 28: Thank you very much for your comments. The most optimal growth environment for Dendrobium ‘Shuijingg’ plantlets, as indicated by the suggestion of various test indexes, is a ratio of 7R:3B.

Comment 29: In general, this study is relevant. However, it is not a new topic. Major modifications are required to be accepted.

Response 29: Thank you very much for your comments. The article has been modified in accordance with the editor's suggestions. If any further improvements are necessary, we will make revisions immediately.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

Dear Authors,

I have read the manuscript concerning Dendrobium hybrid micropropagation as affected by the light quality and my opinion is that it should undergo wide corrections and can be reconsidered and evaluated again after meeting al the issues.

My main concern is that you experiment refer only to single cultivar of a single species which importance or exceptional value was not specified. With this approach the results do not give general overview on the impact of the light treatment on the Dendrobium species, but are cultivar specific.

 

English writing needs improvement, capital/small letters are used improperly (see for example line 48 -name of lamps), tenses are mismatched in several places in the manuscript (eg. 191 - "was observing", 483 "efficiency is found" vs 505 "was found"). Spelling flaws are also present (71 - "coparison"). 

 

M&M as well as Discussion section needs improvement and providing missing information.

What do you mean by "luminous flux density"? How it refers to PPFD? What is the LED lamps manufacturer? What was the distance between FL tubes and plants to obtain 100 umo/m2/s? WHat was the temperature settings in the experimental phase? What were the starting explants? How many plant per treatment? What was the experimental design? Number of repetitions? Give the peak value for lambda max  wavelengths of red and blue light. Fig 1 with lamps characteristics is not legable.

 

Sentences: 107-108, 146 are not clear. 

Sentences 158, 162 are not necessary. 

Line 165 - explain abbreviations when first used.

 

Captions for tables and figures need to be self-explanatory: provide more detailed inforamtion.

 

Discussion on the PCA results is missing.

 

Line 482 - explain QA

 

Editing work needs to be done in the reference list - there are many mistakes. 

 

Supplemetary materials provide the same information as already present in the tables in the main manuscript body (but give more datailed captions, which in fact should be applied in the main body instead of the short ones). In the present form SM are not necessary.

Best regards

Comments on the Quality of English Language

English writing needs improvement, capital/small letters are used improperly (see for example line 48 -name of lamps), tenses are mismatched in several places in the manuscript (eg. 191 - "was observing", 483 "efficiency is found" vs 505 "was found"). Spelling flaws are also present (71 - "coparison"). 

Author Response

1.Comments 1:My main concern is that you experiment refer only to single cultivar of a single species which importance or exceptional value was not specified. With this approach the suggestion do not give general overview on the impact of the light treatment on the Dendrobium species, but are cultivar specific.

Response 1:Thank you very much for your comments. However, due to the species-specific and varietal-dependent nature of light quality, there is currently a lack of research articles specifically addressing the effects on Dendrobium‘Shuijing’, which holds significant implications for production. If any further improvements are necessary, we will make revisions immediately.

Comments 2:English writing needs improvement, capital/small letters are used improperly (see for example line 48 -name of lamps), tenses are mismatched in several places in the manuscript (eg. 191 - "was observing", 483 "efficiency is found" vs 505 "was found"). Spelling flaws are also present (71 - "coparison"). 

Response 2:Thank you very much for your comments, we have modified the wrong expressions and words in the article. line 48 -name of lamps-52, . 191 - "was observing"，202-204, 483 "efficiency is found"We have modified the content of the article, the word has been deleted，we have modified it according to your suggestion.(71 - "coparison"). 74

Comments 3:M&M as well as Discussion section needs improvement and providing missing information.

Response 3:Thank you very much for your comments, we have modified discussion section and provided missing information according to your suggestion.

Comments 4:What do you mean by "luminous flux density"? How it refers to PPFD? What is the LED lamps manufacturer? What was the distance between FL tubes and plants to obtain 100 umo/m2/s? WHat was the temperature settings in the experimental phase? What were the starting explants? How many plant per treatment? What was the experimental design? Number of repetitions? Give the peak value for lambda max  wavelengths of red and blue light. Fig 1 with lamps characteristics is not legable

Response 4: Thank you very much for your comments, we've made changes based on the article， and mark the number of lines of each modification in the following paragraph. About Figure 1 , we've redrawn it to make it more obvious. What do you mean by "luminous flux density"? How it refers to PPFD? 115. What is the LED lamps manufacturer?120. What was the distance between FL tubes and plants to obtain 100 umo/m2/s? 117. What were the temperature settings in the experimental phase?110. What was the experimental design? We have already mentioned the experimental design in 2.2. Number of repetitions?127.Give the peak value for lambda max wavelengths of red and blue light. 119. Fig 1 with lamps characteristics is not legable？

Comments 5: Sentences: 107-108, 146 are not clear. 

Response 5:Thank you very much for your comments, we have modified it in line 107-109,146 according to your suggestion.111-113.

Comments 6:Sentences 158, 162 are not necessary.

Response 6:Agreed. Therefore,we have removed these two sentences from the text at your suggestion.

Comments 7:Line 165 - explain abbreviations when first used.

Response 7:Thank you very much for your reply. We have explained the words in the abstract section.

Comments 8:Captions for tables and figures need to be self-explanatory: provide more detailed inforamtion.

Response 8:Agreed. Therefore, we have modified the captions for tables and figures it according to your suggestion.

Comments 9:Discussion on the PCA suggestion is missing.

Response 9:Agreed. Thank you very much for your comments. We have modified the article according to your suggestion.

Comments 10:Line 482 - explain QA

Response 10:Thank you very much for your suggestion. We have modified the discussion section, and the modified words have been deleted.

Comments 11:Editing work needs to be done in the reference list - there are many mistakes. 

Response 11:Agreed,Therefore,thank you very much for your comments. We have revised and added to the references section

Comments 12:Supplemetary materials provide the same information as already present in the tables in the main manuscript body (but give more datailed captions, which in fact should be applied in the main body instead of the short ones). In the present form SM are not necessary.

Response 12:Agreed. Therefore,thank you very much for your comments. Thank you very much for your suggestion we have changed the table in the text to a figure, the SM part is different from the information in the text and is necessary

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

This paper presents a sound study about the effects of light quality on the performance of Dendrobium plantlets in tissue culture. The research involves interesting biochemical measurements, providing an integrative study on the subject. These results can be used as a basis for the improvement of propagation of other plants in tissue culture.

The paper is well written and clear in general, however, I would advise considering presenting at least part of the data in the form of charts instead of tables. There are minor remarks in the attached file.

Comments for author File: Comments.pdf

Author Response

Comments 1:The paper is well written and clear in general, however, I would advise considering presenting at least part of the data in the form of charts instead of tables. There are minor remarks in the attached file.

Response 1:Agreed. Therefore，thank you very much for your suggestion, we have changed the original table to a figure to show some of the data. The attachments have also been modified

 

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

The manuscript has been improved considerably, however, I left the decision to change the tables of results to graphs to the consideration of the editor-in-chief. In my comments I only mentioned that table 4 (V1) of the manuscript was transformed into a graph and I see that all the tables were transformed. That is why the final decision lies with the editor-in-chief. In manuscript it can be accepted in its current form.