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Open AccessArticle
Genome-Wide Analysis of the TIFY Gene Family in Three Cymbidium Species and Its Response to Heat Stress in Cymbidium goeringii
by
Meng-Meng Zhang
Meng-Meng Zhang 1,2,
Xin He
Xin He 1,2,
Ye Huang
Ye Huang 2,
Qinyao Zheng
Qinyao Zheng 2,
Xuewei Zhao
Xuewei Zhao 1,2,
Linying Wang
Linying Wang 2,
Zhong-Jian Liu
Zhong-Jian Liu 1,2,* and
Siren Lan
Siren Lan 1,2,*
1
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Authors to whom correspondence should be addressed.
Horticulturae 2024, 10(8), 796; https://doi.org/10.3390/horticulturae10080796 (registering DOI)
Submission received: 1 July 2024
/
Revised: 25 July 2024
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Accepted: 26 July 2024
/
Published: 27 July 2024
Abstract
The TIFY family is a plant-specific gene family that is involved in regulating a variety of plant processes, including developmental and defense responses. The Cymbidium species have certain ornamental and ecological value. However, the characteristics and functions of TIFY genes in Cymbidium remain poorly understood. This study conducted a genome analysis of the TIFY gene family in Cymbidium goeringii, C. ensifolium, and C. sinense and investigated their physicochemical properties, phylogenetic relationships, gene structures, and expression patterns under heat stress in C. goeringii. C. goeringii (26), C. ensifolium (19), and C. sinense (21). A total of 66 TIFY genes were identified, and they were classified into four subfamilies (JAZ, ZML, PPD, and TIFY) based on their systematic evolutionary relationships. Sequence analysis showed that TIFYs contained a conserved TIFY domain and that genes within the same subfamily had structural similarity. Analysis of cis-regulatory elements revealed that these genes contain numerous light-responsive elements and stress-responsive elements. We subjected C. goeringii (16 h light/8 h dark) to 24 h of 38 °C high-temperature stress in a climate chamber. Additionally, results from RT-qPCR experiments showed that under heat stress, the expression levels of eight genes in C. goeringii show significant differences. Among them, the JAZ subfamily exhibited the strongest response to heat stress, initially showing upregulation followed by a downregulation trend. In conclusion, this study investigated the role of TIFY genes in three Cymbidium species, providing insights into C. goeringii under heat stress.
Share and Cite
MDPI and ACS Style
Zhang, M.-M.; He, X.; Huang, Y.; Zheng, Q.; Zhao, X.; Wang, L.; Liu, Z.-J.; Lan, S.
Genome-Wide Analysis of the TIFY Gene Family in Three Cymbidium Species and Its Response to Heat Stress in Cymbidium goeringii. Horticulturae 2024, 10, 796.
https://doi.org/10.3390/horticulturae10080796
AMA Style
Zhang M-M, He X, Huang Y, Zheng Q, Zhao X, Wang L, Liu Z-J, Lan S.
Genome-Wide Analysis of the TIFY Gene Family in Three Cymbidium Species and Its Response to Heat Stress in Cymbidium goeringii. Horticulturae. 2024; 10(8):796.
https://doi.org/10.3390/horticulturae10080796
Chicago/Turabian Style
Zhang, Meng-Meng, Xin He, Ye Huang, Qinyao Zheng, Xuewei Zhao, Linying Wang, Zhong-Jian Liu, and Siren Lan.
2024. "Genome-Wide Analysis of the TIFY Gene Family in Three Cymbidium Species and Its Response to Heat Stress in Cymbidium goeringii" Horticulturae 10, no. 8: 796.
https://doi.org/10.3390/horticulturae10080796
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