Encapsulation in Calcium Alginate of Nodes from Stolons of Mentha spicata L.
Round 1
Reviewer 1 Report
The manuscript iw well written. There are minor typos. If the authors can test teh clonal fieldity of the regenerants using molecular markers, the story would have been better built.
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Reviewer 2 Report
- In the abstract, it is necessary to mention the quantitative results as well as the type and concentration of hormones used.
- Why the effects of more or less concentration of growth regulators treatment were not studied?
- In the material method section, it is true that you have given a reference to the encapsulation method, but the method used should be mentioned briefly.
- There are some small typos in the text that need to be corrected, for example in lines 246 and 247, "In vitro" must be italic.
- The authors should compare and discuss the results of the work with the latest results and the most up-to-date references in this field.
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Reviewer 3 Report
What is confected seeds? Elaborate
What is covert in “The synthetic seeds of spearmint showed a satisfactory ability to covert”
Why it was monitored between the 15th and the 56th day from sowing?
In table 2…refer to the first horizontal line, make homogeneity in terms of bracket etc.
Discussion must be improved with citations of new references
The English language must be checked properly
Kindly remove redundancies in the MS.
Most of the things in Table is again represented in the Text
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Reviewer 4 Report
I thought that the data in this manuscript at this time is not very important. The small difference in emergence and growth between the naked nodes and artificial endosperm does not clearly demonstrate the importance of encapsulating in vivo-derived nodes. Also, given that natural nodes are already resistant to microorganisms, it is not surprising that no fungicide is needed for encapsulation. However, I feel that the idea of ​​encapsulation of vivo-derived tissues presented in this manuscript may be the basis for the future development of the synthetic seed field.
One of the great properties of seeds is "long-term storage". This paper may be the basis for developing a system that encapsulates in vivo tissue, arrests growth, stores it, and germinates it at the desired time.
In anticipation of future developments in this area
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Round 2
Reviewer 2 Report
The revision is acceptable.
