Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants

Round 1

Reviewer 1 Report

Dear Authors,

I congratulate you on your work, which I have studied, and I describe my suggestions below:

Abstract: The first word should not be highlighted. The abstract is 244 words - please compress it to a maximum of 200 words.

Keywords: they don't have to start with a capital letter.

2. why is the magnolia highlighted with texture? What is its significance for the draft article. I recommend supplementing this section with other species - even based on the table.

4.12 - is this well indicated in the manuscript? No 4.1. the correct marking?

Discussion: this is missing. Please write the chapter according to MDPI guidelines.

References: this field of science is constantly developing, so I recommend including the relevant literature references of the last 5 years in the text. The applied literature is adequate, but the proportion of new literature is small. I would like to make a significant addition.

Author Response

Dear Reviewer,

 

Thank you for your comments on our article titled, “Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants” (horticulturae-2413981). Furthermore, some minor revisions have also been made to enhance the paper quality. Please see the revised manuscript.

 

Specific comments,

 

1) Abstract: The first word should not be highlighted. The abstract is 244 words - please compress it to a maximum of 200 words.

Reply: We have made corrections according to the reviewer’s comments.

 

2) Keywords: they don't have to start with a capital letter.

Reply: We have made corrections according to the reviewer’s comments.

 

3) why is the magnolia highlighted with texture? What is its significance for the draft article. I recommend supplementing this section with other species - even based on the table.

Reply: We have made corrections according to the reviewer’s comments.

 

4) 4.12 - is this well indicated in the manuscript? No 4.1. the correct marking?

Reply: We have made corrections according to the reviewer’s comments.

 

5) Discussion: this is missing. Please write the chapter according to MDPI guidelines.

Reply: We have included the discussion section in this paper as per the request.

 

6) References: this field of science is constantly developing, so I recommend including the relevant literature references of the last 5 years in the text. The applied literature is adequate, but the proportion of new literature is small. I would like to make a significant addition.

Reply: Some references have been updated as necessary.

 

We hope that our explanations are clear and reasonable.

Thank you again for your consideration.

Luomin Cui

Nanjing Forestry University

Reviewer 2 Report

The manuscript entitled "Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants" provides information about natural occurence of polyploids in various plant genera, polyploidization techniques and typical methods used for detection of (poly)ploidy.
    I believe the authors should substantially improve their article in several ways including language (a), text structure and logical connection between the sentences (b) and better explanation of some topics (c). Plus, there are some minor issues like typos and so on (d).
    
Add a)
    I have found incorrectly constructed sentences scattered throughout the whole manuscript. Therefore, it was sometimes difficult to understand what the authors intended to say. My suggestion is thorough re-reading of the manuscript and using some language editorial service. Here are few examples:
    "And it can also be assumed that the earliest Magnolia denudata and Magnolia liliflora were diploids, diploid-tetraploid-hexaploid, which evolved into polyploids over a long period of cultivation." [The second paragraph in the part 2 (unfortulately numbered lines are missing in the manuscript)]
    "There are few X-rays and ultraviolet mutagenic polyploids because of their low rate." [The first paragraph in the part 3.1]
    "Previous research has shown that polyploidy has 2x x 2x, 2x x 4x, and polyploidy  x polyploidy for intersectional crosses." [The second paragraph in the part 4.1 (probably incorrectly labeled as part 4.12)]
    "The 2n gametophytic pathway to sexual polyploidy has a high frequency of chromosomal rearrangements, and parental trait genes can interpenetrate to achieve progressive breeding of target genes, towards genetic improvement [19]." [The third paragraph in the part 4.1]

Add b)
    Unite information about occurence and frequency of polyploids from the first (Introduction) and the second (Natural occurence of polyploid cells; btw. why cells and not plants or species?) sections of the text.
    Usually is better to start each section with more general information and then provide specific examples. One advantage is that one can avoid repeating the same information several times as it happens, for example, in the section Chemical induction polyploidy/mutation with colchicine. At the beginning of this section is written: "Colchicine is the most commonly used chemical induction for polyploidy..." and in the end of this section is (almost) the same information: "Currently, colchicine is the most widely used chemical inducer...".
    Logical connection between the sentence is wobbling sometimes. Let's take these sentences: "There are limitations to using chromosome preparations to identify plant ploidy. Ploidy identification of 60 Phalaenopsis varieties revealed that most were tetraploid or near tetraploid, however, there were also a few diploids, triploids, tetraploids, and aneuploids [8]." How is the latter sentence linked with the former sentence? The authors should think more carefully about the meaning of their sentences and improve text structuring into sections and paragraphs.

Add c)
    Many information provided by the authors are merely descriptive without any commentary or explanations. For the illustration we can cite this sentence: "There are three main ways to produce 2n gametes: first division restitution (FDR), second division restitution (SDR), and inde-terminate meiotic restitution (IMR) (Fig. 2) [19,78,81]." It's not informative for the reader and I think it deserves at least brief commentary with reference to mentioned articles for more details Moreover, figure 2 is without any notes. Unfortunately, this is not the only case as we can show by another sentence: "Currently, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) molecular markers are also widely used to identify ploidy [116]." is the only information about utilization of molecular markers for ploidy detection. I can provide more examples, but I suppose it is sufficent to show what is the problem.

Add d)
    There is no text reference and commentary to Figure 1. By the way, what is the difference between "Synthetic" and "Artificial" polyploids as we can see on this picture? According to Cheng et al. (2018) are these terms synonyms. When we are talking about synonyms, is the term "unmeiotic" gametes some kind of neologism? I found it only in one article and no one else use it. Further, there is the same problem with Table 3 as with the Figure 1 - neither text reference nor commentary.
    Typos:
Magnolia xsoulangeana -> Magnolia soulangeana (The first paragraph in the section 1)
polyploid is 39 % -> polyploid is 39% (The first paragraph in the section 2)
What is 50 μm for 12 h in the Table 2? Should it be μM? Also, the concentration units     should be the same where possible.
lilies. [19]. -> lilies [19]. (The second paragraph in the section 4)
Feng ([57]) -> Feng et al. [57] (The first paragraph in the section 5)
Henry (2006) -> Henry et al. [115] (The second paragraph in the section 5)


References
Cheng, F., Wu, J., Cai, X., Liang, J., Freeling, M., & Wang, X. (2018). Gene retention, fractionation and subgenome differences in polyploid plants. Nature plants, 4(5), 258-268.

I have found incorrectly constructed sentences scattered throughout the whole manuscript. Therefore, it was sometimes difficult to understand what the authors intended to say. My suggestion is thorough re-reading of the manuscript and using some language editorial service.

 

Author Response

Dear Reviewer,

 

Thank you for your comments on our article titled, “Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants” (horticulturae-2413981). Furthermore, some minor revisions have also been made to enhance the paper quality. Please see the revised manuscript.

 

Specific comments,

 

Add a)

I have found incorrectly constructed sentences scattered throughout the whole manuscript. Therefore, it was sometimes difficult to understand what the authors intended to say. My suggestion is thorough re-reading of the manuscript and using some language editorial service. Here are few examples:

"And it can also be assumed that the earliest Magnolia denudata and Magnolia liliflora were diploids, diploid-tetraploid-hexaploid, which evolved into polyploids over a long period of cultivation." [The second paragraph in the part 2 (unfortulately numbered lines are missing in the manuscript)]

"There are few X-rays and ultraviolet mutagenic polyploids because of their low rate." [The first paragraph in the part 3.1]

"Previous research has shown that polyploidy has 2x × 2x, 2x × 4x, and polyploidy × polyploidy for intersectional crosses." [The second paragraph in the part 4.1 (probably incorrectly labeled as part 4.12)]

"The 2n gametophytic pathway to sexual polyploidy has a high frequency of chromosomal rearrangements, and parental trait genes can interpenetrate to achieve progressive breeding of target genes, towards genetic improvement [19]." [The third paragraph in the part 4.1]

 

Reply: We have revised the sentence according to your suggestion.

 

Add b)

Unite information about occurence and frequency of polyploids from the first (Introduction) and the second (Natural occurence of polyploid cells; btw. why cells and not plants or species?) sections of the text.

Usually is better to start each section with more general information and then provide specific examples. One advantage is that one can avoid repeating the same information several times as it happens, for example, in the section Chemical induction polyploidy/mutation with colchicine. At the beginning of this section is written: "Colchicine is the most commonly used chemical induction for polyploidy..." and in the end of this section is (almost) the same information: "Currently, colchicine is the most widely used chemical inducer...".

Logical connection between the sentence is wobbling sometimes. Let's take these sentences: "There are limitations to using chromosome preparations to identify plant ploidy. Ploidy identification of 60 Phalaenopsis varieties revealed that most were tetraploid or near tetraploid, however, there were also a few diploids, triploids, tetraploids, and aneuploids [8]." How is the latter sentence linked with the former sentence? The authors should think more carefully about the meaning of their sentences and improve text structuring into sections and paragraphs.

 

Reply: We have revised the sentence according to your suggestion.

 

Add c)

Many information provided by the authors are merely descriptive without any commentary or explanations. For the illustration we can cite this sentence: "There are three main ways to produce 2n gametes: first division restitution (FDR), second division restitution (SDR), and inde-terminate meiotic restitution (IMR) (Fig. 2) [19,78,81]." It's not informative for the reader and I think it deserves at least brief commentary with reference to mentioned articles for more details Moreover, figure 2 is without any notes. Unfortunately, this is not the only case as we can show by another sentence: "Currently, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) molecular markers are also widely used to identify ploidy [116]." is the only information about utilization of molecular markers for ploidy detection. I can provide more examples, but I suppose it is sufficent to show what is the problem.

 

Reply: We have incorporated the necessary modifications based on the feedback provided by our homologation reviewers.

 

Add d)

There is no text reference and commentary to Figure 1. By the way, what is the difference between "Synthetic" and "Artificial" polyploids as we can see on this picture? According to Cheng et al. (2018) are these terms synonyms. When we are talking about synonyms, is the term "unmeiotic" gametes some kind of neologism? I found it only in one article and no one else use it. Further, there is the same problem with Table 3 as with the Figure 1 - neither text reference nor commentary.

 

Reply: Fig. 1 has been created based on Manzoor et al. (2019) work and has been modified to include explanatory notes. The use of the term 'unmeiotic' gametes is not a new concept as it has also been mentioned in Li et al. (2022) and Wei et al. (2022). Table 3 and Figure 1 have been updated with additional references and comments.

 

Li, H.M.; Gan, J.C.; Xiong, H.; Mao, X.D.; Li, S.W.; Zhang, H.Y.; Hu, G.B.; Liu, C.M.; Fu, J.X. Production of Triploid Germplasm by Inducing 2n Pollen in Longan. Hortic. 2022, 8, 437.

Manzoor, A.; Ahmad, T.; Bashir, M.A.; Hafiz, I.A.; Silvestri, C. Studies on Colchicine Induced Chromosome Dou-bling for Enhancement of Quality Traits in Ornamental Plants. Plants 2019, 8, 194.

Wei, C., Zhang, R., Yue, Z., Yan, X., Cheng, D., Li, J., Zhang, X. The impaired biosynthetic networks in defective tapetum lead to male sterility in watermelon. Journal of Proteomics, 2021, 243, 104241.

 

Typos:

Magnolia xsoulangeana -> Magnolia soulangeana (The first paragraph in the section 1)

polyploid is 39 % -> polyploid is 39% (The first paragraph in the section 2)

What is 50 μm for 12 h in the Table 2? Should it be μM? Also, the concentration units     should be the same where possible.

lilies. [19]. -> lilies [19]. (The second paragraph in the section 4)

Feng ([57]) -> Feng et al. [57] (The first paragraph in the section 5)

Henry (2006) -> Henry et al. [115] (The second paragraph in the section 5)

 

Reply: Magnolia ×soulangeana is a hybrid lineage variety. The other typpos have been amended as required.

 

We hope that our explanations are clear and reasonable.

Thank you again for your consideration.

Luomin Cui

Nanjing Forestry University

Reviewer 3 Report

“Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants by Cui et al.” reviewed the recent progress and gave an update on the strategy of polyploid induction method, ploidy identification methods, somatic hybridization and sexual polyploidization that were utilized for polyploid breeding in the ornamental plants.

 

1. Some bottlenecks of mutagenesis methods can be included in this review. Some side effects of chemical mutagenesis methods for polyploidy induction including plant chromosome instability issues that happen after haploid induction by colchicine is one of the phenomenons that could be mentioned in the review.  For instance, there may be a trend of decreasing chromosome numbers after polypoid induction by colchicine in successive generations. Also, other side effects include slow or abnormal growth that can be induced by the different concentrations of colchicine in the polyploid induction experiments. What are the advantages of colchicine over amylin, oryzalin, trifluralin, 3-indoleacetic acid and pronamide? 

2. For the issue of mixoploidy, chromosome counting (DAPI staining, cytogenetic analysis) and flow cytometry analysis can be used to determine and confirm the ploidy number and genome sizes. The bottlenecks, drawbacks and limitations can be explained. Further, the method in estimation of nucleus genome size of the polyploidy after polyploidy induction should also be detailed in the review. In particular, more elaboration and brief introduction on QF-PCR (quantitative fluorescence polymerase chain reaction), SSR (simple sequence repeat), and SNP (single nucleotide polymorphism) is necessary.

 

Minor corrections

Page 1, Abstract section, Line 1, the word Polyploid, does not need to be highlighted in bold;

Page 1, Introduction, Polyploidy refers to organisms that possess a complete set of chromosomes from three or more, this sentence does not contain a verb, please include one verb;

Page 1, Introduction, Line 3, has undergone polyploidization instead of polyploidy, grammar error;

Page 2, Line 13, with a view to provide some references, grammar error;

Page 2, Section 2. Natural occurrence of polyploid cells, Line 7, driver of speciation instead of speciate;

Page 3. Section 3. Artificial Induction of Polyploidy in ornamental plant; please capitalize every single words in the heading if necessary;

Page 4, Line 6, spraying instead of praying, grammar error;

Page 7, Line 30-31, 23 triploid hybrid offspring were finally obtained after materials [83], this sentence was incomplete, please rephrase that sentence;

Page 11, Section 6. Conclusions and further prospects, Line 8,  main way to induce instead of induct, grammar error

Author Response

Dear Reviewer,

 

Thank you for your comments on our article titled, “Research Progress of Chromosome Doubling and 2n Gametes of Ornamental Plants” (horticulturae-2413981). Furthermore, some minor revisions have also been made to enhance the paper quality. Please see the revised manuscript.

 

Specific comments,

 

1. Some bottlenecks of mutagenesis methods can be included in this review. Some side effects of chemical mutagenesis methods for polyploidy induction including plant chromosome instability issues that happen after haploid induction by colchicine is one of the phenomenons that could be mentioned in the review. For instance, there may be a trend of decreasing chromosome numbers after polypoid induction by colchicine in successive generations. Also, other side effects include slow or abnormal growth that can be induced by the different concentrations of colchicine in the polyploid induction experiments. What are the advantages of colchicine over amylin, oryzalin, trifluralin, 3-indoleacetic acid and pronamide?

Reply: We have made corrections according to the reviewer’s comments.

 

Although there are several compounds, such as amylin, oryzalin, trifluralin, 3-indoleacetic acid, and pronamide, that can induce polyploidy in plants, colchicine is the most commonly used technique due to its high specificity to plants, broad-spectrum efficacy, and early research adoption.

 

2. For the issue of mixoploidy, chromosome counting (DAPI staining, cytogenetic analysis) and flow cytometry analysis can be used to determine and confirm the ploidy number and genome sizes. The bottlenecks, drawbacks and limitations can be explained. Further, the method in estimation of nucleus genome size of the polyploidy after polyploidy induction should also be detailed in the review. In particular, more elaboration and brief introduction on QF-PCR (quantitative fluorescence polymerase chain reaction), SSR (simple sequence repeat), and SNP (single nucleotide polymorphism) is necessary.

Reply: Chromosome counting through DAPI staining and cytogenetic analysis is the most direct and effective method for detecting mixoploidy. However, this technique can be laborious and some plant chromosomes may be difficult to produce. Alternatively, flow cytometry can be used, but it may be challenging to differentiate between euploid and near euploid.

 

We have made corrections according to the reviewer’s comments.

 

3. Minor corrections

Page 1, Abstract section, Line 1, the word Polyploid, does not need to be highlighted in bold;

Page 1, Introduction, Polyploidy refers to organisms that possess a complete set of chromosomes from three or more, this sentence does not contain a verb, please include one verb;

Page 1, Introduction, Line 3, has undergone polyploidization instead of polyploidy, grammar error;

Page 2, Line 13, with a view to provide some references, grammar error;

Page 2, Section 2. Natural occurrence of polyploid cells, Line 7, driver of speciation instead of speciate;

Page 3. Section 3. Artificial Induction of Polyploidy in ornamental plant; please capitalize every single words in the heading if necessary;

Page 4, Line 6, spraying instead of praying, grammar error;

Page 7, Line 30-31, 23 triploid hybrid offspring were finally obtained after materials [83], this sentence was incomplete, please rephrase that sentence;

Page 11, Section 6. Conclusions and further prospects, Line 8, main way to induce instead of induct, grammar error.

 Reply: We have made corrections according to the reviewer’s comments.

 

We hope that our explanations are clear and reasonable.

Thank you again for your consideration.

Luomin Cui

Nanjing Forestry University

 

Round 2

Reviewer 1 Report

Dear Authors,

thank you for the manuscript corrections, I accept the manuscript.

Author Response

Dear Reviewer,

 

Thank you for your decision and constructive comments on my manuscript.

 

Luomin Cui

Nanjing Forestry University

Reviewer 2 Report

The revised manuscript was improved in some way (link between main text and figures or tables, correction of some sentences, better explanation of 2n games origin), but the authors ignored other suggestions. Therefore, I should mention it again and hope for the best.

a) Language  - sentence structure and mistakes
    Again, the same issue with the sentences. Here are new examples:
"The success rate of physically inducing chromosome doubling in is plants low and there is also a high occurrence of chimeras."

My suggestion: "The success rate of physically inducing chromosome doubling is low in plants and there is also high occurrence of chimeras."

"Generally, chromosome doubling involves physical induction including thermal excitation, radiation, ultrasound, environmental stress, mechanical damage, etc., chromosome doubling involves chemical induction including colchicine, oryzalin, trifluralin, 3-indoleacetic acid, pronamide, etc. [50,51]."

Here we can see two times "chromosome doubling" in one sentence. Maybe, it could be two sentences?

"Colchicine is the most commonly used chemical induction for polyploidy induction (Table 2)."

I suppose colchine is chemical inducer...

    I just gave you another examples in order to illustrate the problem and I have no intention to write full list. Again, as I already wrote you, use some language editorial service.

b) Text structure
    As I mentioned in my previous report, it is better to start each section with more general information and then provide specific studies. Good example is the section 3.1. Chemical induction. It's illogical start with information about specific concentration of colchicine applied on some species and then provide general commentary about chemical induction in the second paragraph.

c) Ethical issue
    Did you really copy pasted part of text of the other reviewer?! I don't know what to say. At least it is ethically disputable. I mean this part: "Some side effects of chemical mutagenesis methods for polyploidy induction including plant chromosome instability issues that happen after haploid induction by colchicine is one of the phenomenons that could be mentioned in the review. For instance, there may be a trend of decreasing chromosome numbers after polypoid induction by colchicine in successive generations. Also, other side effects include slow or abnormal growth that can be induced by the different concentrations of colchicine in the polyploid induction experiments." [The second paragraph in the part 3.2]

d) Minor issues (typos and so on)
 , polyploidy -> Polyploidy [The first paragraph in the part 1]
22 % -> 22% [The first paragraph in the part 2]
Initially, t, seed induction -> Initially, seed induction [The first paragraph in the part 3]
"...and there have been many studies on obtaining polyploids through cell fusion technology [69]." [The first paragraph in the part 3.3] -> Really "many studies"? Citation of only one 18 years old article is not too convincing.
Figure 1 vs Fig. 2, Fig. 3 a,  Fig. 3 b -> Unify it according to journal style
Feng ([57]) -> Feng et al. [57] [The first paragraph in the section 5]
Henry et al. -> Henry et al. [115] [The second paragraph in the section 5]
hybrid progeny. [112]. -> hybrid progeny [112].
By examining the genotype of two markers per chromosome, we were able to deduce the complete karyotype. [The second paragraph in the part 5] -> This sentence begs for citation

The manuscript is readable, but contains mistakes like incorrect word order, typos and so on. The authors should send the manuscript to language editorial service.

Author Response

Dear Reviewer,

 

Thank you for your decision and constructive comments on my manuscript. We do appreciate the reviewers’ comments which are very helpful to improve the manuscript. And we also gave explanations to the comments one by one below.

 

Specific comments,

 

a) Language - sentence structure and mistakes

Again, the same issue with the sentences. Here are new examples:

"The success rate of physically inducing chromosome doubling in is plants low and there is also a high occurrence of chimeras."

My suggestion: "The success rate of physically inducing chromosome doubling is low in plants and there is also high occurrence of chimeras."

"Generally, chromosome doubling involves physical induction including thermal excitation, radiation, ultrasound, environmental stress, mechanical damage, etc., chromosome doubling involves chemical induction including colchicine, oryzalin, trifluralin, 3-indoleacetic acid, pronamide, etc. [50,51]."

Here we can see two times "chromosome doubling" in one sentence. Maybe, it could be two sentences?

"Colchicine is the most commonly used chemical induction for polyploidy induction (Table 2)."

I suppose colchine is chemical inducer...

I just gave you another examples in order to illustrate the problem and I have no intention to write full list. Again, as I already wrote you, use some language editorial service.

 

Reply: Sincere thanks for the careful revision. We have made corrections according to the reviewer’s comments.

 

b) Text structure

As I mentioned in my previous report, it is better to start each section with more general information and then provide specific studies. Good example is the section 3.1. Chemical induction. It's illogical start with information about specific concentration of colchicine applied on some species and then provide general commentary about chemical induction in the second paragraph.

 

Reply: We sincerely thank the reviewer for the positive comments on our manuscript. In response to the reviewer's comments, we have made corrections to the manuscript.  

 

c) Ethical issue

Did you really copy pasted part of text of the other reviewer?! I don't know what to say. At least it is ethically disputable. I mean this part: "Some side effects of chemical mutagenesis methods for polyploidy induction including plant chromosome instability issues that happen after haploid induction by colchicine is one of the phenomenons that could be mentioned in the review. For instance, there may be a trend of decreasing chromosome numbers after polypoid induction by colchicine in successive generations. Also, other side effects include slow or abnormal growth that can be induced by the different concentrations of colchicine in the polyploid induction experiments." [The second paragraph in the part 3.2]

 

Reply: Thanks for the careful revision, and the original word has been corrected. We have revised the sentence.

 

d) Minor issues (typos and so on)

 , polyploidy -> Polyploidy [The first paragraph in the part 1]

22 % -> 22% [The first paragraph in the part 2]

Initially, t, seed induction -> Initially, seed induction [The first paragraph in the part 3]

"...and there have been many studies on obtaining polyploids through cell fusion technology [69]." [The first paragraph in the part 3.3] -> Really "many studies"? Citation of only one 18 years old article is not too convincing.

Figure 1 vs Fig. 2, Fig. 3 a,  Fig. 3 b -> Unify it according to journal style

Feng ([57]) -> Feng et al. [57] [The first paragraph in the section 5]

Henry et al. -> Henry et al. [115] [The second paragraph in the section 5]

hybrid progeny. [112]. -> hybrid progeny [112].

By examining the genotype of two markers per chromosome, we were able to deduce the complete karyotype. [The second paragraph in the part 5] -> This sentence begs for citation

 

Reply: Thanks for the careful revision, and the original word has been corrected.

 

Luomin Cui

Nanjing Forestry University

Reviewer 3 Report

I find that authors have tried to address reviewers’ comments and improved their manuscript.

I would like to suggest the authors consider making changes listed below.

Section 3.2. Chemical induction polyploidy/mutation

Second paragraph

 

Please rephrase the following sentence accordingly to fit into the manuscript text

“Some side effects of chemical mutagenesis methods for polyploid induction including plant chromosome instability issues that could happen after polyploid induction by colchicine is one of the common phenomena that could be mentioned in the review”. 

Author Response

Dear Reviewer,

 

Section 3.2.: We have revised the sentence. Thanks for the careful revision and the original word has been corrected.

 

Luomin Cui

Nanjing Forestry University