Transcriptomic and Metabolomic Analyses of Seedlings of Two Grape Cultivars with Distinct Tolerance Responses to Flooding and Post-Flooding Stress Conditions
Abstract
:1. Introduction
2. Results
2.1. Sequencing Quality Statistics
2.2. Relationship between Samples and Differentially Expressed Genes (DEGs)
2.3. DEGs with Enriched Gene Ontology (GO) Terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways
2.4. Differentially Responsive Transcription Factor Genes
2.5. Differential Metabolites in Response to Flooding and Post-Flooding Stress Conditions
2.6. Integrative Analysis of Transcriptomic and Metabolomic Data
2.7. JA- and ETH-Related Responses of Grapevines to Flooding
2.8. Carbohydrates Metabolism under Flood Stress
2.9. Phytohormone-Mediated Grapevine Responses during Post-Flooding Recovery
2.10. Flavonoid Biosynthesis, Monolignol Biosynthesis, and Endoplasmic Reticulum Associated Degradation in Post-Flooding Recovery
3. Discussion
3.1. Genotype-Specific TF Genes in Flooding and Post-Flooding Response in SO4
3.2. ETH and JA Regulation under Flooding Situation
3.3. The Shift from Aerobic Respiration to Anaerobic Fermentation under Flooding
3.4. JA Mediated ROS Scavenging System under Post-Flooding
3.5. Phytohormone Mediated Plant Growth in Post-Flooding Stage
3.6. Active ERAD in SO4 under Post-Flooding
4. Materials and Methods
4.1. Plants and Experimental Treatments
4.2. Transcriptomic Analysis
4.3. Metabolomic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample Group | Cultivar | Treatment |
---|---|---|
KCK0 | Kyoho | Seedlings sampled before flooding treatment, serving as the control |
KF7 | Kyoho | Seedlings were flooded for 7 days |
KF7R | Kyoho | Seedlings recovered for 3 days after a 7-day flooding treatment |
SCK0 | SO4 | Seedlings sampled before flooding treatment, serving as the control |
SF7 | SO4 | Seedlings were flooded for 7 days |
SF7R | SO4 | Seedlings recovered for 3 days after a 7-day flooding treatment |
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Peng, Y.; Chen, J.; Long, W.; He, P.; Zhou, Q.; Hu, X.; Zhou, Y.; Zheng, Y. Transcriptomic and Metabolomic Analyses of Seedlings of Two Grape Cultivars with Distinct Tolerance Responses to Flooding and Post-Flooding Stress Conditions. Horticulturae 2023, 9, 980. https://doi.org/10.3390/horticulturae9090980
Peng Y, Chen J, Long W, He P, Zhou Q, Hu X, Zhou Y, Zheng Y. Transcriptomic and Metabolomic Analyses of Seedlings of Two Grape Cultivars with Distinct Tolerance Responses to Flooding and Post-Flooding Stress Conditions. Horticulturae. 2023; 9(9):980. https://doi.org/10.3390/horticulturae9090980
Chicago/Turabian StylePeng, Yanjie, Jinli Chen, Wenjie Long, Pan He, Qi Zhou, Xia Hu, Yong Zhou, and Ying Zheng. 2023. "Transcriptomic and Metabolomic Analyses of Seedlings of Two Grape Cultivars with Distinct Tolerance Responses to Flooding and Post-Flooding Stress Conditions" Horticulturae 9, no. 9: 980. https://doi.org/10.3390/horticulturae9090980