Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Lines, Culture, and Treatments
2.3. Cell Survival and Cytotoxicity Assays
2.4. Western Blotting
2.5. Ex Vivo Permeation Study
2.6. Differential Tape Stripping
2.7. Quantification of DPS-2
3. Results
3.1. In Vitro Evaluation
3.1.1. Cell Proliferation and Cytotoxicity Assays
3.1.2. Molecular Analysis by Western Blotting
3.2. Ex Vivo Evaluation
3.2.1. Ex Vivo Permeation Study
3.2.2. Differential Tape Stripping
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | % of Cell Death | ±SD |
---|---|---|
Not treated | 4.0 | 0.4 |
DMSO/DPS-2 5.6 μM | 6.9 | 0.9 |
O/W microemulsion empty | 3.6 | 0.6 |
O/W microemulsion loaded/DPS-2 5.6 μM | 12.2 | 0.6 |
Sample | % of Cell Death | ±SD |
---|---|---|
Not treated | 8.7 | 0.5 |
DMSO/DPS-2 5.6 μM | 9.8 | 0.4 |
O/W microemulsion empty | 9.0 | 0.6 |
O/W microemulsion loaded/DPS-2 5.6 μM | 18.7 | 1.1 |
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Theochari, I.; Ilic, T.; Nicolic, I.; Dobricic, V.; Tenchiou, A.; Papahatjis, D.; Savic, S.; Xenakis, A.; Papadimitriou, V.; Pletsa, V. Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications. Biomimetics 2021, 6, 10. https://doi.org/10.3390/biomimetics6010010
Theochari I, Ilic T, Nicolic I, Dobricic V, Tenchiou A, Papahatjis D, Savic S, Xenakis A, Papadimitriou V, Pletsa V. Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications. Biomimetics. 2021; 6(1):10. https://doi.org/10.3390/biomimetics6010010
Chicago/Turabian StyleTheochari, Ioanna, Tanja Ilic, Ines Nicolic, Vladimir Dobricic, Alia Tenchiou, Demetris Papahatjis, Snezana Savic, Aristotelis Xenakis, Vassiliki Papadimitriou, and Vasiliki Pletsa. 2021. "Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications" Biomimetics 6, no. 1: 10. https://doi.org/10.3390/biomimetics6010010
APA StyleTheochari, I., Ilic, T., Nicolic, I., Dobricic, V., Tenchiou, A., Papahatjis, D., Savic, S., Xenakis, A., Papadimitriou, V., & Pletsa, V. (2021). Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications. Biomimetics, 6(1), 10. https://doi.org/10.3390/biomimetics6010010