Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture, Treatment, and Viability Assay
2.2. Cold Plasma Device Power and Temperature Measurement
2.3. Statistics
3. Results
3.1. The Canady Cold Plasma Conversion System
3.2. Power and Temperature Measurement of CAP
3.3. Cell Viability after CAP Treatment
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgements
Conflicts of Interest
References
- DeSantis, C.E.; Ma, J.; Goding Sauer, A.; Newman, L.A.; Jemal, A. Breast cancer statistics, 2017, racial disparity in mortality by state. CA-Cancer J. Clin. 2017, 67, 439–448. [Google Scholar] [CrossRef] [PubMed]
- Wahba, H.A.; El-Hadaad, H.A. Current approaches in treatment of triple-negative breast cancer. Cancer Biol. Med. 2015, 12, 106–116. [Google Scholar] [PubMed]
- Foulkes, W.D.; Smith, I.E.; Reis-Filho, J.S. Triple-negative breast cancer. New Engl. J. Med. 2010, 363, 1938–1948. [Google Scholar] [CrossRef] [PubMed]
- Liedtke, C.; Mazouni, C.; Hess, K.R.; Andre, F.; Tordai, A.; Mejia, J.A.; Symmans, W.F.; Gonzalez-Angulo, A.M.; Hennessy, B.; Green, M.; et al. Response to neoadjuvant therapy and long-term survival in patients with triple-negative breast cancer. J. Clin. Oncol. 2008, 26, 1275–1281. [Google Scholar] [CrossRef] [PubMed]
- Niemira, B.A.; Boyd, G.; Sites, J. Cold plasma rapid decontamination of food contact surfaces contaminated with salmonella biofilms. J. Food Sci. 2014, 79, M917–M922. [Google Scholar] [CrossRef] [PubMed]
- Schmidt, A.; Woedtke, T.V.; Stenzel, J.; Lindner, T.; Polei, S.; Vollmar, B.; Bekeschus, S. One year follow-up risk assessment in skh-1 mice and wounds treated with an argon plasma jet. Int. J. Mol. Sci. 2017, 18, 868. [Google Scholar] [CrossRef] [PubMed]
- Schmidt, A.; Wende, K.; Bekeschus, S.; Bundscherer, L.; Barton, A.; Ottmuller, K.; Weltmann, K.D.; Masur, K. Non-thermal plasma treatment is associated with changes in transcriptome of human epithelial skin cells. Free Radical Res. 2013, 47, 577–592. [Google Scholar] [CrossRef] [PubMed]
- Pierdzioch, P.; Hartwig, S.; Herbst, S.R.; Raguse, J.D.; Dommisch, H.; Abu-Sirhan, S.; Wirtz, H.C.; Hertel, M.; Paris, S.; Preissner, S. Cold plasma: A novel approach to treat infected dentin-a combined ex vivo and in vitro study. Clin. Oral Investig. 2016, 20, 2429–2435. [Google Scholar] [CrossRef] [PubMed]
- Wu, Y.; Liang, Y.; Wei, K.; Li, W.; Yao, M.; Zhang, J. Rapid allergen inactivation using atmospheric pressure cold plasma. Environ. Sci. Technol. 2014, 48, 2901–2909. [Google Scholar] [CrossRef] [PubMed]
- Volotskova, O.; Dubrovsky, L.; Keidar, M.; Bukrinsky, M. Cold atmospheric plasma inhibits hiv-1 replication in macrophages by targeting both the virus and the cells. PLoS ONE 2016. [Google Scholar] [CrossRef] [PubMed]
- Bußler, S.; Herppich, W.B.; Neugart, S.; Schreiner, M.; Ehlbeck, J.; Rohn, S.; Schlüter, O. Impact of cold atmospheric pressure plasma on physiology and flavonol glycoside profile of peas (pisum sativum “Salamanca”). Food Res. Int. 2015, 76, 132–141. [Google Scholar] [CrossRef]
- Yan, D.; Sherman, J.H.; Keidar, M. Cold atmospheric plasma, a novel promising anti-cancer treatment modality. Oncotarget 2017, 8, 15977–15995. [Google Scholar] [CrossRef] [PubMed]
- Keidar, M. Plasma for cancer treatment. Plasma Sources Sci. Technol. 2015, 24, 20. [Google Scholar] [CrossRef]
- Laroussi, M.; Lu, X.; Keidar, M. Perspective: The physics, diagnostics, and applications of atmospheric pressure low temperature plasma sources used in plasma medicine. J. Appl. Phys. 2017. [Google Scholar] [CrossRef]
- Schlegel, J.; Köritzer, J.; Boxhammer, V. Plasma in cancer treatment. Clin. Plasma Med. 2013, 1, 2–7. [Google Scholar] [CrossRef]
- Tanaka, H.; Ishikawa, K.; Mizuno, M.; Toyokuni, S.; Kajiyama, H.; Kikkawa, F.; Metelmann, H.; Hori, M. State of the art in medical applications using non-thermal atmospheric pressure plasma. Rev. Mod. Plasma Phys. 2017, 1, 89. [Google Scholar] [CrossRef]
- Ishaq, M.; Han, Z.J.; Kumar, S.; Evans, M.D.M.; Ostrikov, K.K. Atmospheric-pressure plasma- and trail-induced apoptosis in trail-resistant colorectal cancer cells. Plasma Processes Polym. 2015, 12, 574–582. [Google Scholar] [CrossRef]
- Adachi, T.; Tanaka, H.; Nonomura, S.; Hara, H.; Kondo, S.; Hori, M. Plasma-activated medium induces a549 cell injury via a spiral apoptotic cascade involving the mitochondrial-nuclear network. Free Radical Biol. Med. 2015, 79, 28–44. [Google Scholar] [CrossRef] [PubMed]
- Weiss, M.; Gumbel, D.; Hanschmann, E.M.; Mandelkow, R.; Gelbrich, N.; Zimmermann, U.; Walther, R.; Ekkernkamp, A.; Sckell, A.; Kramer, A.; et al. Cold atmospheric plasma treatment induces anti-proliferative effects in prostate cancer cells by redox and apoptotic signaling pathways. PLoS ONE 2015. [Google Scholar] [CrossRef] [PubMed]
- Shi, X.; Zhang, G.; Chang, Z.; Wu, X.; Liao, W.; Li, N. Viability reduction of melanoma cells by plasma jet via inducing G1/S and G2/M cell cycle arrest and cell apoptosis. IEEE Trans. Plasma Sci. 2014, 42, 1640–1647. [Google Scholar] [CrossRef]
- Gherardi, M.; Turrini, E.; Laurita, R.; De Gianni, E.; Ferruzzi, L.; Liguori, A.; Stancampiano, A.; Colombo, V.; Fimognari, C. Atmospheric non-equilibrium plasma promotes cell death and cell-cycle arrest in a lymphoma cell line. Plasma Processes Polym. 2015, 12, 1354–1363. [Google Scholar] [CrossRef]
- Volotskova, O.; Hawley, T.S.; Stepp, M.A.; Keidar, M. Targeting the cancer cell cycle by cold atmospheric plasma. Sci. Rep. 2012. [Google Scholar] [CrossRef] [PubMed]
- Ruwan Kumara, M.H.; Piao, M.J.; Kang, K.A.; Ryu, Y.S.; Park, J.E.; Shilnikova, K.; Jo, J.O.; Mok, Y.S.; Shin, J.H.; Park, Y.; et al. Non-thermal gas plasma-induced endoplasmic reticulum stress mediates apoptosis in human colon cancer cells. Oncol. Rep. 2016, 36, 2268–2274. [Google Scholar] [CrossRef] [PubMed]
- Zhao, S.; Xiong, Z.; Mao, X.; Meng, D.; Lei, Q.; Li, Y.; Deng, P.; Chen, M.; Tu, M.; Lu, X.; et al. Atmospheric pressure room temperature plasma jets facilitate oxidative and nitrative stress and lead to endoplasmic reticulum stress dependent apoptosis in HepG2 cells. PLoS ONE 2013. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Zhang, C.; Zhou, Q.Q.; Zhang, X.F.; Wang, L.Y.; Chang, H.B.; Li, H.P.; Oda, Y.; Xing, X.H. Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis. Appl. Microbiol. Biotechnol. 2015, 99, 5639–5646. [Google Scholar] [CrossRef] [PubMed]
- Chung, W.H. Mechanisms of a novel anticancer therapeutic strategy involving atmospheric pressure plasma-mediated apoptosis and DNA strand break formation. Arch. Pharmacal Res. 2016, 39, 1–9. [Google Scholar] [CrossRef] [PubMed]
- Chang, J.W.; Kang, S.U.; Shin, Y.S.; Kim, K.I.; Seo, S.J.; Yang, S.S.; Lee, J.S.; Moon, E.; Baek, S.J.; Lee, K.; et al. Non-thermal atmospheric pressure plasma induces apoptosis in oral cavity squamous cell carcinoma: Involvement of DNA-damage-triggering sub-G1 arrest via the ATM/p53 pathway. Arch. Biochem. Biophys. 2014, 545, 133–140. [Google Scholar] [CrossRef] [PubMed]
- Vandamme, M.; Robert, E.; Lerondel, S.; Sarron, V.; Ries, D.; Dozias, S.; Sobilo, J.; Gosset, D.; Kieda, C.; Legrain, B.; et al. Ros implication in a new antitumor strategy based on non-thermal plasma. Int. J. Cancer 2012, 130, 2185–2194. [Google Scholar] [CrossRef] [PubMed]
- Canady, J.; Shashurin, A.; Keidar, M.; Zhuang, T. Integrated cold plasma and high frequency plasma electrosurgical system and method. U.S. Patent 9,999,462, 19 June 2018. [Google Scholar]
- Weltmann, K.D.; Kindel, E.; von Woedtke, T.; Hähnel, M.; Stieber, M.; Brandenburg, R. Atmospheric-pressure plasma sources: Prospective tools for plasma medicine. Pure Appl. Chem. 2010, 82, 1223–1237. [Google Scholar] [CrossRef] [Green Version]
- Rowe, W.; Cheng, X.; Ly, L.; Zhuang, T.; Basadonna, G.; Trink, B.; Keidar, M.; Canady, J. The canady helios cold plasma scalpel significantly decreases viability in malignant solid tumor cells in a dose-dependent manner. Plasma 2018, 1, 177–188. [Google Scholar] [CrossRef]
- Ly, L.; Jones, S.; Shashurin, A.; Zhuang, T.; Rowe, W.; Cheng, X.; Wigh, S.; Naab, T.; Keidar, M.; Canady, J. A new cold plasma jet: Performance evaluation of cold plasma, hybrid plasma and argon plasma coagulation. Plasma 2018, 1, 189–200. [Google Scholar] [CrossRef]
- Yan, D.; Talbot, A.; Nourmohammadi, N.; Cheng, X.; Canady, J.; Sherman, J.; Keidar, M. Principles of using cold atmospheric plasma stimulated media for cancer treatment. Sci. Rep. 2015. [Google Scholar] [CrossRef] [PubMed]
- Xu, X.; Dai, X.; Xiang, L.; Cai, D.; Xiao, S.; Ostrikov, K. Quantitative assessment of cold atmospheric plasma anti-cancer efficacy in triple-negative breast cancers. Plasma Processes Polym. 2018. [Google Scholar] [CrossRef]
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Cheng, X.; Rowe, W.; Ly, L.; Shashurin, A.; Zhuang, T.; Wigh, S.; Basadonna, G.; Trink, B.; Keidar, M.; Canady, J. Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results. Plasma 2018, 1, 218-228. https://doi.org/10.3390/plasma1010019
Cheng X, Rowe W, Ly L, Shashurin A, Zhuang T, Wigh S, Basadonna G, Trink B, Keidar M, Canady J. Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results. Plasma. 2018; 1(1):218-228. https://doi.org/10.3390/plasma1010019
Chicago/Turabian StyleCheng, Xiaoqian, Warren Rowe, Lawan Ly, Alexey Shashurin, Taisen Zhuang, Shruti Wigh, Giacomo Basadonna, Barry Trink, Michael Keidar, and Jerome Canady. 2018. "Treatment of Triple-Negative Breast Cancer Cells with the Canady Cold Plasma Conversion System: Preliminary Results" Plasma 1, no. 1: 218-228. https://doi.org/10.3390/plasma1010019