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Article

Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study

1
Department of Surgery, University of Washington, Seattle, WA 98195, USA
2
Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
3
Parkland Regional Burn Center, Department of Surgery, University of Texas Southwestern, Dallas, TX 75235, USA
4
Rehabilitation Outcomes Center at Spaulding Rehabilitation Hospital, Department of Physical Medicine and Rehabilitation, Harvard Medical School, Boston, MA 02129, USA
5
Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA
*
Author to whom correspondence should be addressed.
Eur. Burn J. 2024, 5(4), 389-398; https://doi.org/10.3390/ebj5040034
Submission received: 6 August 2024 / Revised: 24 October 2024 / Accepted: 1 November 2024 / Published: 6 November 2024

Abstract

In this study, we aimed to integrate a genetic repository with an existing longitudinal national burn database. We set out two primary objectives, namely (1) to develop standard operating procedures for genetic sample collection and storage, DNA isolation, and data integration into an existing multicenter database; and (2) to demonstrate the feasibility of correlating genetic variation to functional outcomes in a pilot study, using the catechol-O-methyltransferase (COMT) gene. Dubbed the worrier/warrior gene, COMT variants have been associated with varying phenotypes of post-traumatic stress, wellbeing, and resilience. Between August 2018 and July 2020, COMT variants were identified for 111 participants from three sites and correlated with their outcome data. We found no association between COMT variants and functional outcomes, likely due to the inadequate sample size. We also asked all potential participants why they consented to or refused genetic analysis. A thematic analysis of responses revealed altruism and personal interest/enthusiasm in the study as top reasons for consenting. Privacy concerns were the most common reason for refusal. In conclusion, we successfully developed standard operating procedures for genetic sample collection and storage, DNA isolation, and data integration into an existing database, and we demonstrated the feasibility of conducting a multicenter collaborative study using a centralized lab location.
Keywords: genomics; worrier/warrior gene; precision medicine; multicenter database genomics; worrier/warrior gene; precision medicine; multicenter database

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MDPI and ACS Style

Sibbett, S.; Oh, J.; Carrougher, G.; Muffley, L.; Ashford, N.; Pacleb, M.; Mandell, S.; Schneider, J.; Wolf, S.; Stewart, B.; et al. Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study. Eur. Burn J. 2024, 5, 389-398. https://doi.org/10.3390/ebj5040034

AMA Style

Sibbett S, Oh J, Carrougher G, Muffley L, Ashford N, Pacleb M, Mandell S, Schneider J, Wolf S, Stewart B, et al. Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study. European Burn Journal. 2024; 5(4):389-398. https://doi.org/10.3390/ebj5040034

Chicago/Turabian Style

Sibbett, Stephen, Jamie Oh, Gretchen Carrougher, Lara Muffley, Nathaniel Ashford, Maiya Pacleb, Samuel Mandell, Jeffrey Schneider, Steven Wolf, Barclay Stewart, and et al. 2024. "Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study" European Burn Journal 5, no. 4: 389-398. https://doi.org/10.3390/ebj5040034

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