Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study
Abstract
:1. Introduction
2. Materials and Methods
- Surgical intervention to achieve wound closure during the index (acute) hospitalization;
- Those 65 years of age or older with a total body surface (TBSA) equal or greater than 10%;
- Those 0–64 years of age with a TBSA equal or greater than 20%; OR
- All ages with an electrical or lightning burn injury; OR
- All ages with a burn affecting any area of the face, hands, or feet.
2.1. Collection of Genetic Data
- Buccal swab sample collection.
- Sample transport to BMS laboratory.
- Sample storage at −20 °C.
- DNA isolation and quality assessment.
- DNA storage at −80 °C.
- Downstream genetic applications (i.e., COMT SNP analysis).
- Primer:
2.2. Feasibility and Implementation Improvements
2.3. Data Analysis
3. Results
3.1. Feasibility
3.2. Cohort and Correlation
3.3. Thematic Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Count | DNA Concentration (Median {IQR}) | Range | |
---|---|---|---|
Total | 126 | 67.05 {26.175–179.225} | 0–1211.8 |
Site A | 78 | 103.3 {49.8–292} | 2.14–1211.8 |
Site B | 21 | 30.2 {18.8–58.2} | 0–277.8 |
Site C | 27 | 28.5 {14.4–61.8} | 0–181.1 |
DNA sample transported by mail | |||
No | 68 | 169.7 {68.025–347.525} | 0–1211.8 |
Yes | 58 | 33.45 {14.5–77.55} | 0–277.8 |
COMT PCR result | |||
Successful PCR | 111 | 79.5 {33.45–195.7} | 4.02–1211.8 |
Failed PCR | 15 | 8.2 {1.97–25.2} | 0–79.7 |
Total | AA “Worrier” | AG Variant | GG “Warrior” | |
---|---|---|---|---|
All | 111 | 34 | 46 | 31 |
Site A | 75 | 23 | 29 | 23 |
Site B | 15 | 5 | 9 | 1 |
Site C | 21 | 6 | 8 | 7 |
COMT Genotype | ||||
---|---|---|---|---|
AA (n = 34) | AG (n = 46) | GG (n = 31) | p-Value | |
Sex | 0.234 | |||
Male | 27 (36%) | 28 (37%) | 20 (27%) | |
Female | 7 (20%) | 17 (49%) | 11 (31%) | |
Age (median {IQR}) | 47 {36, 58} | 48 {34, 64} | 46 {29, 61} | 0.6968 |
Race (%) | 0.801 | |||
White | 32 (33%) | 38 (39%) | 27 (28%) | |
Black | 1 (33%) | 1 (33%) | 1 (33%) | |
Asian | 0 (0%) | 1 (100%) | 0 (0%) | |
American Indian/Alaskan Native | 0 (0%) | 2 (67%) | 1 (33%) | |
Native Hawaiian or Other Pacific Islander | 0 (0%) | 1 (50%) | 1 (50%) | |
Other | 0 (0%) | 1 (100%) | 0 (0%) | |
Ethnicity (%) | 0.386 | |||
Hispanic/Latino | 4 (24%) | 6 (35%) | 7 (41%) | |
Non-Hispanic/Latino | 29 (33%) | 38 (43%) | 22 (25%) | |
Burn Size (TBSA) (median {IQR}) | 10 {6, 22} | 18 {6, 37} | 13.5 {3, 28} | 0.2322 |
Etiology (%) | 0.289 | |||
Fire/Flame | 24 (32%) | 32 (43%) | 18 (24%) | |
Scald | 1 (50%) | 0 (0%) | 1 (50%) | |
Contact | 0 (0%) | 4 (57%) | 3 (43%) | |
Grease | 4 (27%) | 6 (40%) | 5 (33%) | |
Tar | 0 (0%) | 0 (0%) | 2 (100%) | |
Chemical | 1 (50%) | 1 (50%) | 0 (0%) | |
Electricity | 2 (50%) | 1 (25%) | 1 (25%) | |
Flash | 2 (100%) | 0 (0%) | 0 (0%) |
COMT Genotype | ||||
---|---|---|---|---|
AA | AG | GG | p-Value | |
Outcomes | ||||
VR-12 MCS Preburn | 57 {40, 61} | 59 {52, 62} | 58 {48, 63} | 0.49 |
VR-12 MCS 6-month | 55 {45, 59} | 54 {43, 59} | 57 {45, 63} | 0.28 |
VR-12 PCS Preburn | 53 {49, 56} | 53 {47, 56} | 55 {44, 56} | 0.97 |
VR-12 PCS 6-month | 44 {36, 53} | 45 {32, 55} | 46 {41, 54} | 0.88 |
PTGI 6-month | 33 {17, 44} | 23 {19, 41} | 15 {10, 33} | 0.24 |
Pain Interference (PI) Scores | ||||
PROMIS-29 PI Total | 52 {41.6, 61.2} | 41.6 {41.6, 61.2} | 53.9 {41.6, 55.6} | 0.93 |
VR-12 PI Question | 2 {1, 3} | 2 {1, 3} | 2 {1, 3} | 0.86 |
Theme (n) | Example |
---|---|
Consenting participants (80) | |
Altruism (38) | “Any way I can give back—I am willing” |
Relationship with burn center staff (6) | “You guys are so great here at Harborview, it gets me emotional just thinking about it” |
Interest/enthusiasm (27) | “I find this [research] really interesting” |
Ease of participation (9) | “If I’m not being poked with a needle then I’m happy to” |
Refusals (26) | |
Privacy (14) | “I don’t want to share my genetic info” |
Lack of interest (9) | “Not interested” |
Other (3) | “If I don’t get the results, I don’t want to participate”In response to study procedures: “Sounds pretty weird”Concerns about COVID susceptibility |
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© 2024 by the authors. Published by MDPI on behalf of the European Burns Association. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Sibbett, S.; Oh, J.; Carrougher, G.; Muffley, L.; Ashford, N.; Pacleb, M.; Mandell, S.; Schneider, J.; Wolf, S.; Stewart, B.; et al. Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study. Eur. Burn J. 2024, 5, 389-398. https://doi.org/10.3390/ebj5040034
Sibbett S, Oh J, Carrougher G, Muffley L, Ashford N, Pacleb M, Mandell S, Schneider J, Wolf S, Stewart B, et al. Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study. European Burn Journal. 2024; 5(4):389-398. https://doi.org/10.3390/ebj5040034
Chicago/Turabian StyleSibbett, Stephen, Jamie Oh, Gretchen Carrougher, Lara Muffley, Nathaniel Ashford, Maiya Pacleb, Samuel Mandell, Jeffrey Schneider, Steven Wolf, Barclay Stewart, and et al. 2024. "Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study" European Burn Journal 5, no. 4: 389-398. https://doi.org/10.3390/ebj5040034
APA StyleSibbett, S., Oh, J., Carrougher, G., Muffley, L., Ashford, N., Pacleb, M., Mandell, S., Schneider, J., Wolf, S., Stewart, B., & Gibran, N. S. (2024). Establishing a Collaborative Genomic Repository for Adult Burn Survivors: A Burn Model System Feasibility Study. European Burn Journal, 5(4), 389-398. https://doi.org/10.3390/ebj5040034