**5. Conclusions**

Variability in the *Wx* gene sequences was detected in two *H. chilense* lines representative of the two main ecotypes of species. This gene is located on the long arm of the 7 *Hc<sup>h</sup>* chromosome, opposite to the other *Triticeae* species, which suggests the presence of an inversion between the distal parts of 7*HchS* and 7 *HchL*. Molecular characterization of these alleles showed that this gene is more similar to the *Wx* gene of barley than those of wheat. However, the enzymatic function would be similar in all species and, consequently, the variation present in *H. chilense* could be utilized in wheat breeding through the use of tritordeum as a bridge species.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2073-4395/9/5/261/s1, Figure S1. Alignment of nucleotide sequences of the *Wx* alleles evaluated in this study, Figure S2. Alignment of predicted protein sequences of the waxy proteins evaluated in this study.

**Author Contributions:** J.B.A. conceived and designed the study; J.B.A., L.C., and R.R. performed the experiments; J.B.A. and A.C. analyzed the data and wrote the paper. All authors have read and approved the final manuscript.

**Funding:** This research was supported by grants AGL2014-52445-R and RTI2018-093367-B-I00 from the Spanish State Research Agency (Ministry of Science, Innovation and Universities), co-financed by the European Regional Development Fund (FEDER) from the European Union.

**Conflicts of Interest:** The authors declare no conflict of interest.
