*2.9. DNA Extraction and Sanger Sequencing*

Genomic DNA was extracted from the leaves of N22, the DTY-IL *qDTY* donor, using the Qiagen DNeasy Kit (Qiagen, Limburg, The Netherlands) according to the manufacturer's instructions. LOC\_Os01g67030 (auxin responsive protein) including a 1.8 kb upstream promoter region was amplified using the forward primer GAGCGTGCAGTCCACTAGGCATTATC and reverse primer GTGACACGTATTCTGATGTACTG. The amplicon was cloned into the pGEM-T Easy Vector (Promega, WI, USA), as per manufacturer's instructions and Sanger sequenced using Macrogen, Inc. South Korea as service provider.
