*2.6. PCR Analysis*

All primers used were listed in Table S1. PCR was performed according to standard procedures. The PCR mix for a 20 μL reaction was: Taq 2X Mastermix (NEB): 10 μL; template DNA: 4 μL; primers (10 μM): 0.8 μL each, DMSO: 1 μL, deionised water to 20 μL total volume. For genotyping the *cle7* promoter deletion, the PCR thermal condition was 95 ◦C: 5 min; then 39 cycles of: 94 ◦C: 30 s, 59 ◦C: 30 s, 72 ◦C: 1 min; ending with 72 ◦C 10 min. For SSR analysis, the PCR thermal condition was 95 ◦C: 5 min; then 39 cycles of: 95 ◦C: 30 s, 55 ◦C: 40 s, 72 ◦C: 40 s; ending with 72 ◦C 10 min.
