*2.3. QTL Analysis*

A linkage map of the 'Nong-an'/'Sathi' 98 RIL population comprising 785 SNP markers that were polymorphic between parents was constructed by QTL IciMapping 4.1 [19]. For mapping, Kosambi's function was used, and the Anchor filter option was applied. QTL analysis was conducted by QTL IciMapping 4.1, using inclusive composite interval mapping of additive and epistatic QTLs. Significant LOD threshold value was calculated for each QTL, using 1000 times permutations at *p* = 0.05. The additive e ffect and phenotypic variation explained by each QTL for allelopathy traits were consequently calculated during QTL analysis, using QTL IciMapping 4.1.
