*2.2. Generating E-ML10*

E-ML10 plants (the "Evil-twin" ML10 or "Evil" ML10) were ML10 mutagenized for two successive generations with 0.1% EMS. The first round of EMS was the same as in the generation of the large mutant population in Section 2.1. In the second round, we selected healthy wild-type looking M1 plants, which did not exhibit any visible dominant/deleterious mutant phenotypes, pooled pollen from these plants, treated the pollen with 0.1% EMS and used it to pollinate the sibling M1 plants from which the pollen was harvested. The E-ML10 used for the cross with E1-9 to generate the mapping population was a wild-type looking individual grown from a mixture of seeds after the second round of EMS.
