*4.3. RNA Sequencing of Two F. sylvatica L. Individuals*

Bud samples from three-year-old *F. sylvatica* seedlings (HE1 and HE2) growing in a nursery were collected and immediately put on dry ice. RNA extraction and all further steps were performed by IGA Technology Service in Udine (Udine, Italy). Libraries were prepared using Illumina TruSeq mRNA-seq Kit. Clusters were generated on a flowcell by cBot and sequenced on a HiSeq2000 by using standard Illumina sequencing workflow.
