*4.2. Shoot Induction, Multiplication, and Rooting*

Embryogenic calli were transferred to the RMOS medium. RMOS consists of Murashige and Skoog salts supplemented with thiamine HCl 1.0 mg/L, nicotinic acid 0.5 mg/L, pyridoxin HCl 0.5 mg/L, glycine 2 mg/L, myoinositol 100 mg/L, casein hydrolysate 500 mg/L, and sucrose 30 g/L. Afterward, calli were transferred to RMOS supplemented with 2,4-D (RMSD), 2,4-D and BAP (RMSDB) and 2,4-D, BAP, and kinetin (RMSDBK). The plates were incubated in low light (1400 lux day intensity) following 16:8 h light: dark regime for one week, and then in bright light (2000–2500 lux day intensity) at 26 ± 1 ◦C. The media were solidified using 2.6 g/L phytagel with a pH of 5.7. For multiplication and robust rooting, regenerated plants were transferred to the MSV medium (Murashige and Skoog's basal medium supplemented with IBA).
