*4.5. Tracking Fluorescent Protein (gfp) in Plastids/Cells*

Streptomycin-resistant calli were initially inspected for fluorescence using a hand-held long-wave UV lamp. The proliferating embryos and leaf segments of the putative transgenic sugarcane plants were subjected to fluorescence microscopy using a stereomicroscope equipped with GFP detection (Olympus SZX America, Melville, NY, USA). Sub-cellular localization of GFP was verified by a laser-scanning confocal microscope (Sarastro 2000

Confocal Image System; Molecular Dynamics, Sunnyvale, CA, USA). GFP fluorescence was detected in the FITC (Fluorescein isothiocyanate) channel (488–514 nm) whereas chlorophyll fluorescence was detected in the TRITC (Tetramethyl rhodamine isothiocyanate) channel (560–580 nm).
