*3.11. Cell Culture*

BEAS-2B (human bronchial epithelial cells, ATCC CRL-9609, Manassas, VA, USA) and A-549 (human lung carcinoma, BCRC 60074, Hsinchu, Taiwan) were obtained from the ATCC (American Type Culture Collection) (Manassas, VA, USA) and the BCRC (Bioresource Collection and Research Center) (Hsinchu, Taiwan), respectively. A-549 cells were cultured in complete Ham's F12K medium and BEAS-2B was maintained in complete DMEM medium. The complete medium was prepared by supplementing plain medium with 10% FBS, 100 μg/mL streptomycin, and 100 units/mL penicillin. All cells were cultured in a 37 ◦C humidified 5% CO2 atmosphere. The cells were passaged every 2–3 days.
