3.2.2. Determination of Monosaccharide Composition

Monosaccharide composition of LJSF4 was analyzed by a 6890 N gas chromatographic system (Agilent 6890 N, Agilent, Santa Clara, CA, USA) after hydrolysis and acetylation according to the method described by Zha et al. [43].

#### *3.3. E*ff*ect of LJSF4 on UVB-Irradiated HaCaT Cells*

#### 3.3.1. HaCaT Cells Culture and UVB Irradiation

HaCaT cell line was obtained from Korean Cell Line Bank (Seoul, Korea). Cells were cultured in DMEM supplemented with 100 μg/mL of streptomycin, 100 unit/mL of penicillin, and 10% FBS at 37 ◦C in humidified atmosphere with 5% CO2.

Cells were exposed to UVB irradiation at wavelength of 280–320 nm by a UVB meter (UV Lamp, VL-6LM, Vilber Lourmat, Paris, France). Cells were irradiated by UVB at a dose of 30 mJ/cm<sup>2</sup> [40,42] and incubated until analysis.
