4.4.1. OMM-1

Oxidative stress in the uveal melanoma cell line OMM-1 was induced by treatment with 1 mM H2O2 as previously shown [39]. The appropriate H2O2 concentration to lower cell viability of OMM-1 cells to nearly 50% after 24 h was previously determined [19] and applied for the assessment of protective effects of fucoidans (1, 10, 50 and 100 μg/mL). Fucoidans were applied 30 min before stress insult. In parallel, cells were treated with the extracted fucoidans only (1, 10, 50 and 100 μg/mL) to determine possible anti-proliferative effects. To measure cell viability, a MTS-assay was performed as described below.
