*4.1. Materials*

HT-29, MCF-7, Hela, and 293T cells were purchased from Kunming Institute of Zoology, Chinese Academy of Sciences Kunming Cell Bank; Dulbecco's minimum essential medium (DMEM) was purchased from GE Healthcare HyClone, USA; trypsin was purchased from Invitrogen, US; penicillin and streptomycin were purchased from Invitrogn, USA; Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Biotechnology, China; Ddimethylsulphoxide (DMSO) was purchased from Sigma-Aldrich, USA; fucoidan from *F. vesiculosus* was provided by Shandong University, where the average molecular weight of fucoidan is about 1300 kDa, as analyzed by size exclusion chromatography (fucose content is about 80%, galactose is about 9%, glucuronic acid is about 7%, glucose is about 2%, xylose is about 1%, and rhamnose is about 1%, which were detected by high performance liquid chromatography (HPLC) after acid hydrolysis) [30,31]; Cytochrome C inhibitor was purchased from abcam, UK; cell cycle and apoptosis analysis kit was purchased from Beyotime, China; mitochondrial membrane potential assay kit with JC-1 was purchased from Beyotime, China; Transwell chamber (8.0 μm) was purchased from corning, USA; primary antibodies against Bcl-2 (product number: AB112), BAX (product number: AB026), P-JNK/SAPK (product number: AJ516), JNK/SAPK (product number: AJ518), caspase-8 (product number: AC056), caspase-9 (product number: AC062), GAPDH (product number: AG019), and cytochrome C (product number: AC909) were purchased from Beyotime, China; primary antibodies against DR4 (product number: 24063-1-AP) and caspase-3 (product number: 66470-2-Ig) were purchased from Proteintech, USA; horseradish peroxidase-conjugated anti-rabbit (product number: A0208) and anti-mouse (product number: A0216) secondary antibodies were purchased from Beyotime, China; diluent was purchased from Beyotime, China; siRNA (DR4) was purchased from Sangon Biotech, China; Lipo8000 transfection reagen<sup>t</sup> was purchased from Beyotime, China; PCR primer was purchased from Sangon Biotech, China; revert aid first strand cDNA synthesis kit was purchased from ThermoFisher, USA; and dream taq green PCR master mix (2X) was purchased from ThermoFisher, USA.
