*4.7. Cell Culture*

The MCF-7 cells were cultured in DMEM media and HL-60, and Vero cells were cultured in RPMI media, both of which were supplemented with 10% FBS and 1% penicillin/streptomycin mixture. Cell cultures were maintained under 37 ◦C in a humidified atmosphere supplemented with 5% CO2. The cells under exponential growth were seeded for the experiments. The cancer cells were seeded in a concentration of 1 × 10<sup>5</sup> cells mL−<sup>1</sup> in 96 well culture plates for 24 h and treated with di fferent concentrations of the samples. After incubating for 24 h, the MTT assay was carried out for the determination of cell viability [30].
