*4.8. Statistical Analysis*

Data are presented as the mean ± standard error of the mean (SEM). The difference between groups was analyzed using SPSS software (v.20.0; IBM, Armonk, NY, USA) via one-way ANOVA with Student's *t*-test. And, Tukey's honest significant difference test was used for the analysis of multiple comparisons. Difference was considered to be statistically significant between various groups when *p* < 0.05. The results were interpreted using GraphPad Prism software (v.7.0; GraphPad Software Inc., San Diego, CA, USA).
