*4.4. Cell Cycle Analysis*

After treatment with fucoidan, the cells were harvested with trypsin and washed twice with cold phosphate bu ffer saline (PBS). Subsequently, the cell nuclei were stained using the cell cycle and apoptosis analysis kit following the manufacturer's instructions. The cells were analyzed by flow cytometry and the relative DNA content was determined using the Modfit software (5.0.9., Verity, Topsham, VT, USA).
