*3.5. Blood Biochemical Indexes*

Serum was separated and the levels of serum creatinine, blood urea nitrogen (BUN), total protein, albumin, total cholesterol, and total triglyceride were analyzed with Beckman biochemical analyzer. The protein of the kidney was determined using Coomassie Brilliant Blue (G-250) by the method of Bradford using a bovine serum albumin (BSA) standard.

#### *3.6. Assessment of MDA and SOD Levels*

The kidney levels of SOD and MDA were analyzed using kits from NanJing JianCheng (NanJing JianCheng Bio Inst, China), and the protocols were all followed the introduction of the kit.
