*4.5. Methyl Thiazolyl Tetrazolium (MTT)-Assay*

The established cell viability assay MTT [40] was performed as previously described [13,19] and was applied after three days of stimulation with fucoidans, after collection of the supernatant for VEGF-content assessment. The biological material was washed with PBS and cultivated with 0.5 mg/mL MTT for 2 h. After removal of MTT, cells were treated with dimethyl sulfoxide. Color formation was measured at 550 nm with an Elx800 instrument (BioTek Instruments Inc., Bad Friedrichshall, Germany).
