4.4.2. ARPE-19

The appropriate TBHP concentration to lower cell viability of ARPE-19 cells to nearly 50% after 24 h (0.5 mM TBHP) was previously determined [19] and applied for the assessment of protective effects of fucoidans (1, 10, 50 and 100 μg/mL). Fucoidans were applied 30 min before stress insult. In parallel, cells were treated with only the extracted fucoidans (1, 10, 50 and 100 μg/mL) to determine anti-proliferative effects. To measure cell viability the MTS-Assay was performed as described below.
