*4.6. Data Analysis*

Results are expressed as mean ± SE. The data obtained were checked against Shapiro-Wilk normality test, which found that all the *P* values were larger than 0.05, and the statistical significance

was determined by Student's *t*-test using GraphPad Prism ver. 6.0 for Mac OSX software (GraphPad Software, La Jolla, CA, USA).

**Author Contributions:** J.A., K.N., T.S., S.T., and H.K. contributed to the study design. J.A., T.U., M.M., A.Y., H.O., S.S.-T., A.I.-H., and S.T. contributed to data collection. J.A., S.T., and H.K. contributed to data analysis. J.A., S.T., and H.K. contributed to data interpretation. J.A., S.T., and H.K. contributed to manuscript preparation. All the authors contributed to revising and approval of manuscript content.

**Funding:** This research was funded by JSPS KAKEN Grants JP15K10682 and JP19K09829.

**Acknowledgments:** This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (JSPS: 23659161), Japan, JSPS KAKEN Grants JP15K10682 and JP19K09829, and the Japan Science and Technology Agency (JST: 18•06255) and is partial fulfillment by Juria Akasaka of the degree of Doctor of Medical Science at Nara Medical University.

**Conflicts of Interest:** The authors declare no conflict of interest.
