*4.5. RNA Interference (RNAi)*

Small interfering RNA (siRNA) directed against human*RAGE* was synthesized by NGRL. The sense sequence of siRNA for human *RAGE* was 5-AUCUACAAUUUCUGGCUUCtt-3 (corresponding to 466-484 of NM\_001136) as described [76,78]. The Silencer® Select human scrambled siRNA was purchased from Ambion® (Waltham, MA, USA) and used as a control. Transfection of siRNAs to SW872 cells was carried out using Lipofectamine® RNAiMAX Reagent (Life Technologies, Waltham, MA, USA) as described [68,72,74–80]. Cells were transfected with 5 pmol siRNA per 24-well culture dish (4.0 × 10<sup>5</sup> cells/mL in 24-well plates).
