*4.12. Statistical Analysis*

To calculate the protein fold change percentage (FC%), the average value of the normalized protein ratio of the PE group was divided over that of the normotensive samples. Also, the normalized protein ratios were used in the Student's *t*-test to calculate the statistical significance of the observed FC. Considering the low variability and high sensitivity of the TMT-MS, a FC ≥ 20% and *p* value ≤ 0.05 was determined as a significant difference in protein expression between PE and normotensive samples.

GraphPad Prism (version 7, San Diego, CA, US) was used to perform Mann Whitney U Test and to prepare the graphs.
