3.3.1. Hematoxylin and Eosin (HE) Staining

(HE) staining of hepatic tissues of control and all treated rats are demonstrated in Figure 1. As it can be observed, the figure shows the hepatoprotective effect of FLE and DLE after CCl4 hepatotoxin administration.

**Figure 1.** Pathological histology analysis in rat liver tissues after HE staining (magnification: 200×): (**A**) controls, (**B**) CCl4-treated (CCl4); (**C**) FLE-treated (FLE), and (**D**) CCl4-treated co-treated with FLE (CCl4 + FLE). (**E**) DLE-treated (DLE), and (**F**) CCl4-treated cotreated with DLE (CCl4 + DLE). Each microphotograph shows a section of an individual liver. Extensive focal necrosis ( ), a moderate Kupffer cell hyperplasia ( ), congestion and dilatation of the central vein (Cg) and leukocyte infiltration (LI).

The control rats' hepatic sections revealed a normal form of hepatic cells; polyhedral hepatocytes and sinusoids boundaries exhibited a single layer of fenestrated endothelial and Kupffer cells (Figure 1A). FLE and DLE alone had no significant effects on liver histology (Figure 1C,E) whilst hepatoxin administration resulted in cells injury (Figure 1B). This was marked by extensive focal necrosis, a moderate Kupffer cell hyperplasia, congestion and dilatation of the central vein, and leukocyte infiltration. Finally, following the administration of CCl4 + FLE or CCl4 + DLE, focal neutrophils were infiltrated in some space and a number of centrilobular veins were congested (Figure 1D,F).
