**Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage**

**Amani Taamalli 1,2, Anouar Feriani 3, Jesús Lozano-Sanchez 4,5,\*, Lakhdar Ghazouani 3, Afoua El Mufti 3, Mohamed Salah Allagui 6, Antonio Segura-Carretero 4,5, Ridha Mhamdi <sup>2</sup> and David Arráez-Roman 4,5**


Received: 21 May 2020; Accepted: 16 June 2020; Published: 18 June 2020

**Abstract:** Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl4) in rat models. For this purpose, healthy albino rats of 180–250 g weight were used. The assessment of biochemical markers was carried out on blood and liver tissue. Then, a histopathological study was carried out on liver tissue. The obtained results showed that fresh and dried olive leaf extracts ameliorate the perturbed biochemical parameters caused by CCl4 treatment. Furthermore, the results registered for the histopathological study are in accordance with the biochemical parameters and the protective capacity of SC-CO2 extracts against DNA damage, indicating that olive leaf extracts helped to improve liver fibrosis caused by CCl4 treatment.

**Keywords:** olive leaves; supercritical fluid extraction; antioxidants
