*2.2. Skin Conditions*

The aim of this study was to evaluate the effects of CPO on skin conditions including elasticity, moisture, and TEWL. These skin conditions were measured at baseline and after 8 weeks of supplementation (Table 2). TEWL was significantly attenuated in the CPO group compared with the placebo group after 8 weeks of supplementation. For skin elasticity and VISIA analysis, no statistically significant differences were observed between raw values for CPO and placebo groups. However, placebo group showed a decrease of elasticity, from 0.818 ± 0.05 at baseline to 0.779 ± 0.06 after 8 weeks of supplementation. On the other hand, CPO groups showed an increase of elasticity, from

0.766 ± 0.07 at baseline to 0.784 ± 0.07 after 8 weeks of supplementation (Table 2). By analyzing the changes from the baseline, we observed statistically significant differences between the placebo and CPO groups (−0.039 ± 0.047 in placebo group, and 0.018 ± 0.065, Figure 2A). In addition to elasticity, a significantly reduced change in the number of skin pores was also observed after 8 weeks of supplementation in the CPO group compared with the placebo group (Figure 2B).


**Table 2.** Score of skin conditions.

**Figure 2.** Dietary supplementation with collagen peptide and ornithine increased skin elasticity, and decreased the number of pores. Change in skin elasticity from baseline (**A**), and change in the number of pores from baseline (**B**). \* *p* < 0.05 and \*\* *p* < 0.01 by unpaired *t*-test. Error bars indicate SD.

In addition, only the placebo group showed significantly decreased collagen scores compared with baseline using the DermaLab test (placebo group, from 45.9 ± 17.9 to 40.9 ± 14.6, *p* = 0.027; CPO group, from 41.1 ± 15.2 to 38.9 ± 13.4, *p* = 0.097). No statistically significant differences in moisture or superficial pH were observed between CPO and placebo groups.
