2.1.3. Effect of Hydrolysis Time on the Extraction Yield of PSC

To check the effect of hydrolysis time on the extraction yield of PSC, different hydrolysis time (4, 6, 8, 10 and 12 h) was carried out in this study. The other two extraction parameters were set as follows: pepsin concentration was 1200 U/g and solid-liquid ratio was 1:55 in 0.5 M acetic acid buffer. As shown in Figure 1c, the extraction yield of PSC was significantly increased with hydrolysis time between 4 and 8 h. Therefore, the hydrolysis time of 8 h was selected for the next optimization.
