*4.5. MTT Staining*

To detect the distribution and amount of viable cells, medium of cell-seeded monophasic and biphasic scaffolds was supplemented with 1.2 mM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma), followed by further incubation at 37 ◦C for 4 h. Cell seeded scaffolds were imaged using a Leica stereomicroscope.
