3.8.2. Osteogenic Differentiation

The MBMS and MC3T3E1 cells with a density of 5 × <sup>10</sup><sup>4</sup> cells/well were seeded in 6 well microtiter culture plate and cultured with osteoblast differentiation medium composed of growth supplements (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China) for 21 days as reported in our previous protocol [13]. The culture medium was replaced every three days.
