*4.10. Histological Investigations on Biphasic Scaffolds*

Biphasic scaffolds cultivated for 21 days under osteochondral stimulation were fixed with 4% buffered formaldehyde, dehydrated and embedded in paraffin. 5 μm sections were cut and mounted to cover slides. After deparaffinization, sections were stained with hematoxylin/eosin (H/E) to visualize cell distribution and toluidine blue to visualize the production of cartilage proteoglycans. Stained samples were images using a BZ-9000 (Biorevo) (Keyence, Neu-Isenburg, Germany) microscope.
