*3.6. Tissue Culture*

Mouse mesenchymal 3T3-L1 cells were seeded in a growth medium consisting of Dulbecco's modified Eagle's medium (450 mg/dL; Biological Industries, Beit Haemek Ltd., Beit Haemek, Israel), 10% fetal bovine serum (Biological Industries), 1% L-glutamine (Biological Industries), and 0.1% penicillin-streptomycin (Sigma, St. Loius, MO, USA). Cell morphology was analyzed using ImageJ software (National Institute of Health, Bethesda, MD, USA).

**Acknowledgments:** The Israeli Ministry of Science (53441 grant to DB and RHA) supported this study. We thank The Interuniversity Institute for Marine Sciences in Eilat (IUI) for assistance and use of facilities. We acknowledge M. Weis for assistance, Z. Barkay for ESEM work, V. Wexler for digital editing, and N. Paz for editorial assistance. We are grateful to the anonymous reviewers for their constructive comments that greatly contributed to the manuscript.

**Author Contributions:** M.S., D.B., and R.H.-A. conceived and designed the experiments; M.S., L.P., and L.A. performed the experiments; M.S. and L.A. analyzed the data; D.B., Y.B., and R.H.-A. contributed reagents/materials/analysis tools; D.B., M.S., L.P., and Y.B. wrote the paper. Authorship is limited to those who contributed substantially to the work reported.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**


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