3.8.1. Cell Culture

Mouse bone marrow-mesenchymal stem (MBMS) and MC3T3E1 cells were received from the cell bank (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China) and were cultured in stem cell culture medium and DMEM, respectively, comprising 10% FBS and antibiotics (1% penicillin/streptomycin) at 37 ◦C in a CO2 incubator. Mesenchymal stem cell growth supplement was added with the culture medium to induce MBMS cell growth.
