3.8.3. Proliferation Assay

After differentiation, the cells were trypsinized using EDTA/Trypsin solution and re-seeded in 96 well plates. Then the cells were treated with 1, 10, and 50 ng/mL concentration of blue shark skin collagens. Cells grown without collagen samples served as the control. The proliferative effect of collagen on bone cells was measured using a cell counter (Invitrogen, Countess II Automated Cell Counter, ThermoFisher Scientific, Shanghai, China) at 3 days after treatment.
