**5. Conclusions**

In this study, PCS, ACS, BCE I, and BCE II were successfully fabricated and evaluated for its utility as dermal substitute. PCS, ACS, BCE I, and BCE II treatment increased the wound healing ability, fibroblasts proliferation, collagen synthesis, re-epithelialization and dermal reconstitution in vivo that owed to the biomimetic structure and high porosity of the collagen nanofibers. This study indicated that PCS, ACS, BCE I, and BCE II could accelerate wound healing rapidly and effectively. The overall results of this study suggest that collagen from tilapia and electrospun bovine collagen nanofibers can be used as a promising dermal substitute to treat severe wounds.

**Author Contributions:** Conceptualization, N.L. and W.W.; Data curation, J.C. and K.G.; Formal analysis, N.L.; Funding acquisition, J.E., B.B. and W.W.; Investigation, J.C., K.G. and B.B.; Methodology, J.E. and J.D.; Project administration, W.W.; Resources, S.L.; Software, J.C.; Supervision, W.W.; Validation, S.W. and W.W.; Visualization, W.W.; Writing—original draft, J.C. and K.G.; Writing—review & editing, J.E., N.L. and W.W.

**Funding:** This research was funded by the National High Technology Research and Development Program of China [grant number 2011AA09070109], the National Natural Science Foundation of China [grant number 81341082 and 81750110548], the Plan of Innovation Action in Shanghai [grant number 15410722500], and the Jiangsu Provincial Natural Science Foundation [grant number BK20151282]. And the APC was funded by the National Natural Science Foundation of China [grant number 81750110548].

**Acknowledgments:** The authors acknowledge the financial support from the National High Technology Research and Development Program of China, the National Natural Science Foundation of China, the Plan of Innovation Action in Shanghai and the Jiangsu Provincial Natural Science Foundation.

**Conflicts of Interest:** The authors declare no conflict of interest.
