*3.6. Protein Conformation*

Circular dichroism (CD) spectra of extracts were recorded at 180 to 280 nm on a Jasco J-1500 dichrograph (Jasco Corp., Tokyo, Japan) using a 0.1-cm path length cuvette. Dry collagen was dissolved at 0.1 mg/mL in 5 mM phosphate buffer pH 3. Samples were loaded at 4 ◦C into precooled CD cuvettes.
