*4.1. Preparation of Biphasic Scaffolds*

Biphasic but monolithic collagen scaffolds were prepared by overlaying suspensions of biomimetically mineralized salmon collagen and fibrillized jellyfish collagen in the cavities of a 96-well plate. Biomimetically mineralized collagen and fibrillized jellyfish collagen suspensions were prepared as already published [7,9]. After freezing of the overlayed samples at 1 K/min to a final temperature of −20 ◦C lyophilization was conducted for about 24 h (Alpha 1–2, Christ). For stabilization the scaffolds were cross-linked with a 30 g/L solution of *N*-(3-dimethylaminopropyl)-*N* -ethylcarbodiimide (EDC) hydrochloride in 80% *v*/*v* ethanol for 12 h. Subsequently, the scaffolds were rinsed thoroughly in deionized water, 1% glycine solution, once again in water and finally freeze-dried.
