2.1.2. Effect of Solid-Liquid Ratio on the Extraction Yield of PSC

Different solid-liquid ratio (1:25, 1:35, 1:45, 1:55 and 1:65) was used to study the effect of solid-liquid ratio on the extraction yield of PSC. The other two extraction parameters were set as follows: pepsin concentration was 1200 U/g and hydrolysis time was 8 h in 0.5 M acetic acid buffer. As shown in Figure 1b, the extraction yield of PSC was significantly increased with increasing liquid-solid ratio between 1:25 and 1:55. So, the solid-liquid ratio of 1:55 was selected for next optimization.
