*3.6. Denaturation Temperature (Td)*

The denaturation temperature of collagen from tilapia skin was determined by differential scanning calorimetry (DSC) (Netzsch DSC 200PC, Selb, Bavaria, Germany). A collagen sample with a concentration of 5 mg/mL was dissolved in 0.05 M of acetic acid and sealed in an aluminum pan for scanning. Then, the endothermal curve from 10 ◦C to 50 ◦C was obtained at a rate of 5 ◦C/min in a nitrogen atmosphere.
