*4.5. Blood Sampling and Safety Evaluation*

Serum was obtained at baseline and after 8 weeks of supplementation, and blood IGF-1 levels were evaluated. To analyze the increases of plasma growth hormone levels, serum was obtained at 0, 60, 120, 150, 180, and 240 min after ingestion of test food. Plasma IGF-1 and growth hormone levels were measured by LSI Medience Co, Ltd. (Osaka, Japan) General biochemical examination of blood and hematologic tests were performed for safety evaluation.
