*4.7. Statistical Analysis*

Values are shown as the mean ± SEM. Significant differences between the mean values of the two groups were evaluated by Student *t*-test with IBM SPSS Statistics 23. Statistical significance was indicated by a *p* value < 0.05.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/10 .3390/md19050278/s1, Table S1: Effect of fish oil (FO) supplementation on gene expression in liver.

**Author Contributions:** T.Y. designed the research; R.I., D.L. and T.Y. conducted the research, and T.Y. and R.I. analyzed the data. T.Y. and R.I. wrote the manuscript with contributions from D.L. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported in part by a JSPS Grant-in-Aid for Scientific Research (C) Grant Number 16K01855.

**Institutional Review Board Statement:** Care of the mice followed guidelines of the National Institutes of Health's Guide for the Care and Use of Laboratory Animals. The National Institutes of Biomedical Innovation, Health and Nutrition, Japan, reviewed and approved all animal procedures (Approval no. DS27-52R3).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are included in the corresponding sections throughout the manuscript.

**Conflicts of Interest:** The authors declare that they have no conflict of interest.
