*4.5. Serum Chemistry*

Blood was obtained from the mice, and serum glucose was measured with an Ascensia autoanalyzer (Bayer Medical, Ltd., Tokyo, Japan). Serum levels of NEFA, TG and TC were measured by enzymatic colorimetry with NEFA C, TG E and TC E test kits (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively.
