*4.8. Identification within the in Silico Proteome of T. lutea of PKS Enzymes Involved PUFA Synthesis*

The in silico proteome generated from last annotated version of the genome of *T. lutea* was used to identify putative proteins involved in n-3 PUFA PKS pathways [66]. We used the PKS previously identified in the haptophyte *Chrysochromulina tobin* as query for a BLASTp analysis, using e-value <10−<sup>3</sup> as threshold [67]. Analysis of conserved domain was performed using the NCBI CD database V3,18 with e-value <10−<sup>2</sup> as threshold. The genome location of genes encoding selected proteins was identified to evaluate genes' proximity and occurrence of gene clusters. Proteins and cluster of proteins containing the four domains ketoacyl reductase (KR), dehydrase/dehydrogenase (DH), enoyl reductase (ER), and polyketide synthase (KS) were selected as candidates.
