*2.1. Identification and Classification of Hsf and Hsp Family Members in Eggplant*

Published Arabidopsis *Hsf* and *Hsp* sequences [23] were retrieved and used as queries in BLAST searches against the eggplant genome database (http://eggplant.kazusa.or. jp/, accessed on 6 June 2021) to identify potential eggplant *Hsfs* and *Hsps*. All output genes identified according to Arabidopsis *Hsf* and *Hsp* sequences were collected and confirmed using Pfam (http://pfam.xfam.org/search, accessed on 6 June 2021) and SMART (http://smart.embl-heidelberg.de/, accessed on 6 June 2021). The isoelectric points and molecular weights were predicted using the Compute pI/Mw tool from ExPASy (http: //web.expasy.org/compute\_pi, accessed on 6 June 2021).
