*3.2. Genetic Map Construction*

After four-step filtering, our final map contained 5565 markers on 12 Chrs, which were designated Chr01-Chr12 using HighMap software and presented in Table 2 and Figure 2. The linkage map encompassed a total of 1535.69 cM and exhibited an average marker distance of 0.28 cM (Figure 2). The largest Chr was Chr03, which consisted of 444 markers, showed a length of 169.18 cM, and an average marker-to-marker distance of 0.38 cM, while the smallest Chr was Chr05 that consisted of 460 markers, showed a length of 99.98 cM, and an average marker-to-marker distance of 0.22 cM (Table 3). The extent of linkage between markers was represented by the percentage of "Gaps ≤ 5 cM," which ranged from 99.08% to 100%, and an average of 99.70% (Table 3). The largest gap on this linkage map was situated on Chr10 at 9.99 cM, whereas the smallest gap was 3.94 cM at Chr08 (Table 3).

**Figure 1.** Specific-locus amplified fragment sequencing (SLAF) polymorphism analysis. Marker count in eight segregation patterns. The *x*-axis represents eight segregation patterns for the polymorphic SLAF markers, and the *y*-axis shows the number of markers.
