*2.7. High-Density Linkage Map Construction*

We quantified the modified logarithm of odds (MLOD) value between two adjacent markers and markers with MLOD values < 5 were filtered out. Then, the SLAF markers were assigned to chromosomes (Chr), and 12 Chr were obtained. Simultaneously, we analyzed the linear array of markers in every Chr using HighMap software [46] and then estimated the genetic distances between a pair of adjacent markers.
