*3.2. Phylogenetic and Sequence Structure Analysis of Hsf and Hsp Proteins in Eggplant*

To evaluate the evolutionary relationship of the eggplant Hsf and Hsp proteins, a phylogenetic analysis of each family was performed based on the full-length amino acid sequences from Arabidopsis, eggplant and tomato and each family could be classified into different subfamilies. The *SmHsf* family contained three subfamilies: type A (18 genes), type B (5 genes) and type C (1 gene). Based on the phylogenetic tree, class HsfA had the maximum number of subclasses among the three classes and was closer to tomato Hsf proteins, which coincided with the botanical classification (Table S2). A total of 39 *sHsp* genes could be grouped into 12 distinct subfamilies, containing 6 groups of cytosolic *sHsp* genes, C-I, C-II, C-III, C-IV, C-V and C-VI and 2 groups of mitochondrial *sHsp* genes, MT I and MT II. Notably, the C-I *sHsp* group in the eggplant genome was large, containing 24 genes, compared with 6 in Arabidopsis (Table S3). The *Hsp60* family was divided into 4 subfamilies, including cytosol-localized Cpn60 (12 genes), mitochondrion-localized Hsp60

(4 genes) and chloroplast-localized Cpn60-a (2 genes) and Cpn60-b (3 genes) (Table S4). The *Hsp70* family contains genes encoding 19 cytosolic *Hsp70s*, 4 binding proteins (BIPs, Hsp70 homologs in the ER), 3 mitochondrial Hsp70s (mtHsc70s) and 2 chloroplastid Hsp70s (cpHsc70s) (Table S5). Seventeen *Hsp90* family genes could be divided into cytoplasm (Cyt), mitochondrial (MT), endoplasmic reticulum (ER) and chloroplast, containing 8, 3, 2 and 1 proteins, respectively (Table S6). The Hsp100 family can be classified into ClpB, C, D and X classes as follows: 3 ClpB proteins (designated as B1, B2 and B3), 4 ClpC proteins (C1, C2, C3 and C4), 1 ClpD protein (D1) and 2 ClpX proteins (X1 and X2) (Table S7).
