*3.1. Inheritance of Head Color of BT 126 with SN 60*

Significant differences in head color were observed among the BT 126 (parent 1, P1, purple head), SN 60 (parent 2, P2, green head), and F1 hybrids planted in 2018 and 2019 (Figure 1). The BT 126 purple line exhibited a high anthocyanin accumulation in the heads (5.72 mg/g FW) and developed normally when compared to green broccoli, under normal conditions, in the field and greenhouse [4]. It was crossed to SN 60, with very low anthocyanin content (0.241 mg/g FW), to generate the F1 progeny. F1 individuals displayed third grade color. As expected, the F2 population was divided into purple and green classes, with varying degrees of continuous distribution in 1st–5th color grades (Figure 1E–I). No transgressive segregation was observed in either direction of the parental genotype in the mapping population. We could infer that the purple trait was controlled by QTL.
