*Review* **CRISPR-Cas9 Gene Editing for Fruit and Vegetable Crops: Strategies and Prospects**

**Lili Wan 1, Zhuanrong Wang 1, Mi Tang 1,\*, Dengfeng Hong 2, Yuhong Sun 1, Jian Ren 1, Na Zhang <sup>1</sup> and Hongxia Zeng <sup>1</sup>**


**Abstract:** Fruit and vegetable crops are rich in dietary fibre, vitamins and minerals, which are vital to human health. However, many biotic stressors (such as pests and diseases) and abiotic stressors threaten crop growth, quality, and yield. Traditional breeding strategies for improving crop traits include a series of backcrosses and selection to introduce beneficial traits into fine germplasm, this process is slow and resource-intensive. The new breeding technique known as clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 (Cas9) has the potential to improve many traits rapidly and accurately, such as yield, quality, disease resistance, abiotic stress tolerance, and nutritional aspects in crops. Because of its simple operation and high mutation efficiency, this system has been applied to obtain new germplasm resources via gene-directed mutation. With the availability of whole-genome sequencing data, and information about gene function for important traits, CRISPR-Cas9 editing to precisely mutate key genes can rapidly generate new germplasm resources for the improvement of important agronomic traits. In this review, we explore this technology and its application in fruit and vegetable crops. We address the challenges, existing variants and the associated regulatory framework, and consider future applications.

**Keywords:** CRISPR/Cas; gene knockout; genome editing; germplasm resource; precision editing; regulatory framework; trait improvement
