*2.4. Data Statistics and Analysis Results*

The scoring was given as 1 (presence) and 0 (absence) for the amplified fragments in each microsatellite loci, and data matrixes were constructed accordingly. Then, employing Popgen (version 1.32) software, several indices were calculated, such as the observed number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and the Shannon information index (I) [36]. The major allele frequency (MAF), polymorphism information content (PIC), and gene diversity index were calculated using PowerMarker (version 3.0) software [37]. In addition, the cluster analysis of the germplasms was based on the Nei genetic distance [38] and a neighbor joining (NJ) method was used to construct a dendrogram via PowerMarker (version 3.0) software. The dendrogram tree was visualized and edited using MEGA7 (version 7.0) [39].
