**3. Understanding the Genetic Basis of Biotic Resistance in Vegetable Crops**

Fungal diseases remain challenges restricting the sustainable development of vegetable production. Although pesticides can prevent and control fungal diseases, excessive

**Citation:** Wang, Y.; Zhang, Y. Advances in Molecular Breeding of Vegetable Crops. *Horticulturae* **2022**, *8*, 821. https://doi.org/10.3390/ horticulturae8090821

Received: 17 August 2022 Accepted: 25 August 2022 Published: 7 September 2022

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use of pesticides has brought great damages to the environment and human beings. Improving disease resistance has become an important breeding objective. The development and establishment of molecular marker technology makes it fast and effective to select germplasm resources directly.

The development of molecular markers associated with resistance to gray mold disease in onion (*Allium cepa* L.) through RAPD-PCR was assessed by Kim et al. [8]. RAPD analysis was performed to identify the genetic relationship between the resistant and susceptible lines and develop the SCAR marker. In addition, RNA-seq of the gray mold-resistant and -susceptible onion lines were analyzed to develop a selectable marker for the resistant line.

*Phytophthora* blight is a common disease that causes decreased yield and quality in pepper (*Capsicum annuum* L.). Li et al. generated a high-resolution genetic map of pepper associated with resistance to *Phytophthora capsici* by SLAF-seq and QTL analysis [9]. *CQPc5.1* was identified as a major quantitative trait locus (QTL) for the *P. capsici* resistance, including 23 candidate genes located within the interval.
