*2.2. RAPD and Phylogenetic Analysis*

The gDNA of each line was used for a RAPD analysis to identify the genetic relationship between the resistant and susceptible lines. For a RAPD analysis, OPERON random primers OPAN-1~OPAN-20 and OPL-1~OPL-20 were used. The sequences of the 40 OPERON random primers are shown in Table S2. The RAPD PCR was performed in 20 μL by using the Maxime PCR Premix (iNtRON Biotechnology, Seongnam, Korea) containing 2.5-U i-TaqTM *Taq* polymerase, 2.5-mM dNTPs, 1X reaction buffer with 10 pmol primer, and 50 ng of gDNA. The amplification program was as follows: denaturation at 95 ◦C for 10 min, 40 cycles of 1 min at 95 ◦C, 1 min at 37 ◦C, 1 min at 72 ◦C, and a final extension at 72 ◦C for 10 min. The amplified RAPD PCR product was electrophoresed at 100 mA for 3 h in a 1% agarose gel. The polymorphic bands obtained from the RAPD-PCR of each line were converted into binary data, depending on the presence of a band; the presence of a polymorphic band was scored as 1, while its absence was scored as 0. The dendrogram was obtained using the unweighted pair group method (UPGMA) with an arithmetic mean [22] by using the Jaccard coefficient [23] through the XLSTAT program (Addinsoft, New York, NY, USA).
