*2.5. Evaluation of the Clinical Tolerance to the Biscuits Labelled without Milk and Egg*

Within one month of inclusion in the study, the selected patients underwent SPT and OFC with milk and egg –free biscuits "Magretti" [Galbusera S.p.A., Cosio Valtellino (SO), Italy] from 12 different lots. Such biscuits are labelled as "not containing milk and egg". However, the label indicates "it cannot be excluded that any traces of these allergens are present, in any case less than 5 mg/kg" (Table 1).

Based on the age of patients, seven or eight incremental doses of food were administered at 20-min intervals under clinical supervision. The first six doubling doses, starting from 0.25 g up to 8.00 g, were the same for all study participants. The seventh and eighth doses differed in different age groups (Table S1). A sample of the biscuits used for the OFC and SPT was stored for proteomic evaluation. A symptoms-based clinical score assessing the degree of gastro-intestinal, respiratory, cardiovascular and dermatological reactions was applied to monitor the acute allergic reactions. The procedure was interrupted when clear-cut objective symptoms were present, or after any severe, persistent (over 40 min) subjective symptom, according to our stopping rules [17].


**Table 1.** Magretti biscuit composition.
