*4.7. Bioinformatics*

Bioinformatic analyses were performed as previously described [9,10]. Briefly, database searches for sequence homology were performed using the programs BLASTp (http: //www.ncbi.nlm.nih.gov/BLAST/, accessed on 19 June 2021) and FASTA (http://www. genome.jp/tools/fasta/, accessed on 19 June 2021) set to standard parameters. The putative cell localization of AstaP was predicted using PSORT (http://psort.hgc.jp/form.html, accessed on 19 June 2021), SignalP (http://www.cbs.dtu.dk/services/SignalP/, accessed on 19 June 2021) and TargetP (http://www.cbs.dtu.dk/services/TargetP/, accessed on 19 June 2021). The *O*-linked glycosylation of Ser and Thr residues was predicted by the program NetOGlyc 3.1 (http://www.cbs.dtu.dk/services/NetOGlyc/, accessed on 19 June 2021). The *N*-linked glycosylation site was predicted by the program NetNGlyc 1.0 (http://www.cbs.dtu.dk/services/NetNGlyc/, accessed on 19 June 2021). The isoelectric point (pI) and molecular mass were predicted by GENETYX-MAC software (GENETYX Corporation, Tokyo, Japan).
