*2.6. Differential Expression of DlWRKY Genes in Response to Stress and Hormonal Treatments*

The expression patterns of 55 *DlWRKY* genes were investigated in response to hormonal and various stresses by using qRT-PCR. As shown in Figure 6 and Figure S1, the majority of the *DlWRKY* genes (44 of 55) were up-regulated or down-regulated by >2-flod under at least one tested treatment, while eleven genes (*DlWRKY*2, 6, 12, 13, 14, 19, 29, 33, 34, 40, and 50) showed no significant differential expression in response to the given treatments. The SA treatment induced the expression of the 22 *DlWRKY* genes (*DlWRKY*1, 3, 5, 8, 10, 15, 16, 18, 23, 26, 27, 32, 36, 38, 42, 43, 45, 46, 48, 51, 54, and 55) but reduced the expression of five *DlWRKY* genes (*DlWRKY*9, 20, 24, 25, and 41). Fifteen *DlWRKY* genes (*DlWRKY*1, 3, 4, 8, 10, 16, 21, 32, 38, 39, 44, 45, 51, 53, and 55) were up-regulated, and three (*DlWRKY*20, 25, and 41) were down-regulated by MeJA treatment. For heat treatment, 11 (*DlWRKY*4, 9, 20, 27, 28, 35, 37, 39, 44, 49, and 52) and 4 (*DlWRKY*5, 8, 16, and 51) genes were down-regulated or up-regulated, respectively. A total of 17 *DlWRKY* (*DlWRKY*5, 7, 9, 17, 18, 20, 23, 25, 26, 31, 37, 39, 41, 42, 47, 51, and 54) genes showed up-regulated expressions, and no genes were down-regulated by cold treatment. Under the drought treatment, 20 (*DlWRKY*1, 4, 5, 8, 10, 11, 15, 16, 17, 21, 22, 25, 26, 27, 28, 30, 36, 45, 48, and 51) and 7 *DlWRKY* genes (*DlWRKY*9, 35, 37, 41, 44, 49, and 54) were up-regulated or down-regulated, respectively. Eighteen (*DlWRKY*1, 4, 5, 8, 10, 11, 15, 16, 18, 21, 23, 32, 36, 38, 42, 45, 48, and 51) and five *DlWRKY* genes (*DlWRKY*9, 20, 24, 37, and 41) were up-regulated or down-regulated, respectively, under high salinity treatment.
