*4.9. Expression Analysis of Physic Nut Dof Genes*

The original expression data for *JcDof* genes under different treatments (including gibberellins [GA], 6-Benzylaminopurine[BA], high salt concentration and drought) were retrieved from NCBI SRA database (Available online: https://www.ncbi.nlm.nih.gov/). All the data were analyzed using Tuxedo suite (TopHat and Cufflinks, http://post.queensu.ca/~rc91/NGS/TuxedoTutorial.html) and then upper-quartile normalized and log transformed. Heat maps were generated by means of

the HemI toolkit (Available online: http://hemi.biocuckoo.org/) with average linkage hierarchical clustering [60,61].
