*4.3. Exon–Intron Structure Analysis, Conserved Motifs and Domain Architecture*

The gene structures (exon–intron) of CaChi were obtained by aligning the CDS sequences with their corresponding genomic sequences, and their structures were shaped by using online Gene-Structure-Display Server 2.0 as described by Kang et al. (2016) [83]. Conserved motifs of the CaChi genes were recognized using MEME tool (4.12.0) (Available online: http://meme-suite. org/tools/meme) as described by Guo et al. (2016) [84] with maximum number of motifs = 10. To increase the confidence level, all candidate protein sequences were further scrutinized for presence of the functional domains by the online tools, Conserved Domain Database (CDD) (Available online: http://www.ncbi.nlm.nih.gov/cdd/), SMART (Available online: http://smart.embl-heidelberg.de/), and EMBL-EBI (Available online: https://www.ebi.ac.uk/interpro/), and their diagrams were generated using online EXPASY server (Available online: https://prosite.expasy.org/mydomains/).
