*2.2. Phylogenetic Analysis of DlWRKY*

A phylogenetic tree was constructed using the maximum likelihood (ML) method and based on multiple alignments of longan, grape, and *Arabidopsis* WRKY domain aa sequences. As shown in Figure 1, the phylogenetic results revealed that all the DlWRKY proteins could be categorized into three groups (I, II, and III). Eleven DlWRKY proteins were considered to be group I, which included two WRKY domains and a C2H<sup>2</sup> (C–X4–C–X22–23–HXH) zinc finger motif. A total of 35 DlWRKY proteins contained one WRKY domain and a C2H<sup>2</sup> (C–X4–5–C–X23–HXH) zinc-binding motif, which were classified as group II. The nine remaining genes were assigned to Group III, which consisted of a single WRKY domain and a C2CH (C–X7–C–X23–HXC) zinc-binding motif. According to the WRKY subgroup classification of *Arabidopsis*, the DlWRKYs in Group II were further subdivided into five subgroups, including groups IIa (3), IIb (7) IIc (13), IId (6), and IIe (6).
