*4.5. Differential Expression Analysis*

Differential expression analysis of the sample groups was performed using DESeq [65] to obtain a set of DEGs between the drought group and the control group, and the False Discovery Rate (FDR) was used to correct the *p*-value of the multiple-hypothesis test. In this study, FDR < 0.01 and FC (Fold Change) ≥ 4 were set as the threshold for significantly differential expression, and could be used to prepare for the follow-up study on the drought stress of Verbena.
