*4.9. RNA-seq Expression Analysis*

Publicly available RNA-seq data were retrieved from the expVIP [50] were used to analyze the expression pattern of predicted wheat *EXO70* genes in different tissues and stresses. The tissue-specific expression data were compiled from three wheat tissues (leaf, stem, root) collected from Chinese Spring at seeding development. The biotic stress expression data included two diseases (stripe rust and powdery mildew pathogen) and were collected from disease-resistant wheat varieties N9134 (at 7 days seedling stage). The abiotic stress expression data, including drought and heat treatments, were collected from heat-resistant wheat cultivar TAM107 (at 7 days seedling stage). The relative expression of each *TaEXO70* gene in different tissues and stresses was presented as a heat map, which was constructed by the heat map drawing software MeV (version No. 4.7, Institute for Genomic Research, MD, USA).

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/20/1/60/s1.

**Author Contributions:** X.W., J.X., H.W. and J.Z. conceived and designed the study; J.Z., X.Z., W.W. and J.X. analyzed the data; H.Z., J.L. and M.L. collected the plant materials; J.Z. and X.Z. performed the experiments; J.Z., X.W. and J.X. wrote the manuscript. All authors reviewed and edited the manuscript.

**Funding:** This work was supported by the National Key Research and Development program (2016YFD0101004, 2016YFD0102001-004), the National Science Foundation of China (Nos. 31471490, 31661143005, 31290213), the Important National Science & Technology Specific Projects in Transgenic Research (2014ZX0800907B), the Chinese High Tech Program of China (No. 2011AA1001), the Program of Introducing Talents of Discipline to Universities (No. B08025), the 333 Talent Project of Jiangsu Province, the Shanghai Agriculture Applied Technology Development Program, China Grant (No. Z201502) and Development of Key Agricultural Varieties in Jiangsu Province (PZCZ201706).

**Conflicts of Interest:** The authors declare no conflict of interest.
