*4.5. Chromosomal Location and Genes-Duplication Analysis of CaChi Genes*

Information about the chromosomal location of CaChi genes were obtained from Pepper Genome Platform (PGP) (Available online: http://peppergenome.snu.ac.kr/) as described by Zhang et al. (2016) [61], and the genes were mapped on chromosomes using MapDraw [87]. Duplication analysis within the pepper genome was carried out with the criteria depicted by Gu et al. (2002) [88] further detail as: (1) the FASTA-alignable region among the two proteins should be more than 80% of the longer protein sequence; and (2) the identity between the two protein sequences (I) should be I ≥ 30% if the alignable region is longer than 150 amino acid and I <sup>≥</sup> 0.01*<sup>n</sup>* + 4.8L−0.32(1 + exp(−L/1000) otherwise, where *n* = 6 and L is the alignable length between the two protein sequences of the gene [88,89].
