*4.5. Subcellular Localization*

The coding region of *ZmWRKY106* was fused to the subcellular localization vector p16318h with green fluorescent protein (GFP) tags containing the CaMV35S promoter. The specific primers are listed in Supplementary Table S1. For transient expression assays, the p16318h-*ZmWRKY106* reconstruction plasmid was transformed to maize mesophyll protoplasts by the PEG-mediated method, while the p16318hGFP vector was transformed as control [52]. The fluorescence signals were observed by a confocal laser scanning microscope (LSM700; CarlZeiss, Oberkochen, Germany) after incubation in darkness at 22 ◦C for 18 h.
