*4.1. Identification of Longan WRKY Genes*

Longan whole-genome sequences, transcript data, and proteins were downloaded from the NCBI Sequence Read Archive (SRA315202) or ftp://climb.genomics.cn/pub/10.5524/100001\_101000/ 100276/ [37]. The HMM profile of the WRKY DNA binding domain (PF03106) which was extracted from the Pfam database (http://pfam.sanger.ac.uk/) was used to obtain the potential members of the longan WRKY genes [59] and used to search the putative WRKY genes from the longan genome with HMMER 3.0 (http://hmmer.janelia.org/) with the default parameters and 0.01 as the cutoff value. Then, all non-redundant longan WRKY protein sequences were selected and the domain was conserved using Simple Modular Architecture Research Tool (http://smart.emblheidelberg.de/) [60].
