*4.7. Phylogenetic Analysis of EXO70 Gene Family*

Multiple sequence alignment was conducted by ClustalW which was integrated in Mega v6.0 [88]. Phylogenetic analysis was performed through online software PhyML 3.0 [89] using maximum-likelihood method with default parameter [90]. EXO70 proteins are rather diverse at their N-terminal and could not be aligned reliably, so we only used the conserved domain proteins to construct the phylogenetic tree and removed five genes where the length of the domain is fewer than 200 amino acids for further analysis.
