*4.3. Protein Feature Analysis*

The ExPASy online tools (http://expasy.org/tools/) [63] were used to calculate the MW, the number of amino acids, the ORF, ORF length, and isoelectric point (pI) of DlWRKY proteins. The arrangements and the intron and exon junctions of the *DlWRKY* genes were analyzed by the GSDS, version 2.0 [64]. MEME (http://meme.sdsc.edu/meme/cgi-bin/meme.cgi) [65] was used to analyze the conserved motifs of the DlWRKY proteins with the following optimized parameters: any number of repetitions; maximum number of motifs: 15; and the optimum width of each motif: between 6 and 50 residues.
