*4.11. Statistical analysis*

The results were subjected to an analysis of variance (ANOVA) using SPSS software (SPSS version 23.0, SPSS Inc., Chicago, IL, USA), and the analyzed data were expressed as means ± standard deviation (SD) of three replications in all measured parameters. The least significant difference (LSD; *p* < 0.05) test was used to measure the significant differences among the given treatments. Data were presented in graphs and designed using GraphPad Prism 7.0 (GraphPad Software, Inc., LA Jolla, CA, USA).
