*2.2. Tissue-Specific Gene Expression*

In order to analyze the expression of *PheDof12-1* in different tissues (root, stem, leaf, flowering leaf, flower) and floral organs (pistil, stamen, embryo, glume, palea, flower bud, bract), RNA was isolated to perform qRT-PCR. The results show that the transcription level of *PheDof12-1* in flowering leaf is significantly higher than in other tissues. In different flower organs, the expression of *PheDof12-1* was highest in palea, and lowest in bract (Figure 1C). In developing flowers, *PheDof12-1* had higher transcript accumulation at the floral bud formation stage (F1) (Figure 1D), and decreased gradually at flower development, which was consistent with the previously reported detection of *PheDof1* at early stages of flower formation and development [29]. We further generated *ProPheDof12-1*-*GUS* transgenic lines, and glucuronidase (GUS) staining was detected in the vasculature of cotyledons and hypocotyls, true leaves, roots, flower, and pollen (Figure 1E,F). The results demonstrate that *PheDof12-1* is expressed in different tissues and at different flower development stages, suggesting that it is dynamic during plant development and may play an important role in moso bamboo growth and development.
