*2.2. GO Analysis of Warming- and Minor Warming-Responsive Genes*

To identify genes associated with acclimation or adaptation to HT, we functionally classified upregulated genes in Kenshin under warming conditions, in Kenshin under both warming and minor-warming conditions, and in both lines under warming and minor-warming conditions (Tables S12–S14) via GO analysis (Table 2). Unexpectedly, no gene was identified in the "heat acclimation" or "response to heat" category among genes only expressed in Kenshin in response to warming conditions, but these categories were enriched among upregulated genes in both lines. Only one "heat acclimation"-related gene (*BrCYP71B2*) was identified in Kenshin under both minor-warming and warming conditions. However, several genes in putative HT adaptation-related gene categories were identified, such as 6 "heat acclimation", 13 "response to heat", and "chromosome organization" genes, among genes upregulated by warming and minor warming in both lines. Genes in the "heat acclimation" category included *BrHSP18.2*, *BrROF2* (FKBP-type peptidyl-prolyl *cis-trans* isomerase family protein), *BrHSP20-L*, *BrHSFA2*, *BrMge1* (*Mitochondrial GrpE2*), and an unknown gene. Genes in the "response to heat" category included most sHSPs, and "chromosome organization" genes included *BrHON4*, *BrSMP1* (*Swellmap 1*), *BrSWIB*, *BrENTG/VHS* family protein, and *BrSWC6* (SWR1 complex subunit 6) (Table S14). Categories that were specifically enriched in Kenshin under warming conditions included "lipid biosynthetic process", "response to hormone stimulus" (most were auxin responsive), "intracellular membrane bound organelle" (most of unknown function), and "signal transduction" (most were defense related) (Table S12).
