*4.4. Analysis of Cis-Regulatory Elements and Gene Ontology of CaChi*

To analyze the *cis*-acting elements, 1500 bp upstream from the start codon (ATG) of the CaChi were obtained from the pepper genome database (PGD) and queried against the PlantCARE (Available online: http://bioinformatics.psb.ugent.be/webtools/plantcare/html/) [85] and Neural Network Promoter Prediction (Available online: http://promotor.biosino.org/) online servers. The gene ontology analysis of CaChi protein sequences were obtained from Blast2GO (Available online: http://www.blast2go.com) program [86].

The amino acid sequences were blast in Blast2GO program and three groups of GO classification (molecular functions, biological process and cellular component) were recovered.
