*4.2. Phylogenetic Analysis*

A multiple alignment was performed with the full-length amino acid sequences of the rice trihelix family proteins using MEGA v. 7.0 (https://www.megasoftware.net/) [46]. Unrooted trees were constructed by the maximum-likelihood (ML) method with the following parameters: Poisson correction; pairwise deletion; 1000 bootstrap replicates.
