*2.2. Unique Alleles*

Among the 382 alleles detected in the studied accessions, 123 alleles were found and were termed as unique alleles (Supplementary Table S3). A high percentage (17.51%) of unique alleles was observed in *G. ekmanianum* genotypes (Table 1). Twenty-five unique alleles were found in two accessions of *G. hirsutum*. Nineteen, 13, 18, 13, and 4 unique alleles were observed in *G. barbadense*, *G. tomentosum*, *G. darwinii*, *G. klotzschianum,* and *G. stephensii*, respectively. *G. ekmanianum* had the highest number (31) of unique alleles, which were collected from the Dominican Republic, National Plant Germplasm System (NPGS) USA (Supplementary Table S3). These unique alleles are an important genetic resource for cotton and have never been studied before.

**Table 1.** Summary of unique (present in one accession) and rare alleles (present in <5% accessions) observed in a combined Panel of 132 accessions.

