*4.4. Identification of EXO70 Gene Families in Wheat and Related Triticeae Species*

We searched for the keywords 'exocyst subunit exo70 family protein' in the annotated proteins database of *Hordeum vulgare* (HH, 2*n* = 2x = 14, accession No. FJWB02000000) and obtained entries containing gene ID and protein sequences [42]. Then we performed a Conserved Domains (CD) search (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) and reserved the protein sequences that contain the typical pfam03081 domain [47].

The identified EXO70 protein sequences of *H. vulgare* were used as query sequences to blast (*E*-value <sup>≤</sup> <sup>10</sup>−10) against protein database of the other species, including *Triticum urartu* (AA, 2*<sup>n</sup>* = 2x = 14, accession No. NMPL02000000) [39], *T. aestivum* (AABBDD, 2*n* = 6x = 42, accession No. NMPL02000000) [80], *Brachypodium distachyon* (BdBd, 2*n* = 2x = 14, accession No. ADDN03000000) [81], Oryza sativa Japonica (2*n* = 2x = 24, accession No. AP008207–AP008218) [82], *T. dicoccoides* (AABB, 2*n* = 4x = 28, accession No. FXXJ01000000) [43] and *Aegilops tauschii* (DD, 2*n* = 2x = 14, accession No. AOCO02000000) [83]. After removing the redundant gene sequences for each the species, the alignment hits were validated by performing a CD search as described above.
