*4.4. Phylogenetic Analysis*

Physic nut, *A. thaliana*, and *R. communis* Dof protein sequences were pretreated by GUIDANCE2 online tool to remove unreliable columns [47]. The phylogenetic relationship among the Dof proteins was analyzed using ClustalW and the dendrogram was constructed using MEGA (v6.0, Tokyo Metropolitan University, Tokyo, Japan) by neighbor-joining method, with the following parameters: Poisson correction, pairwise deletion, and 1000-bootstrap replicates [48].
