*4.8. Sequence Alignments and Prediction of Transmembrane Domains of SlUGlcAE Family*

All nine SlUGlcAE and six AtUGlcAE protein sequences were aligned using the (version 5.0.6, North Carolina State University, Raleigh, NC, USA), and then the results were output by genedoc program. Next, the hydrophilicity of the SlUGlcAE protein sequences was predicted by the trans-membrane Hidden Markov model algorithm (available online: http://www.cbs.dtu.dk/ services/TMHMM/) [52].
