*2.1. Identification of WRKY Gene Family in Longan*

To extensively identify the WRKY genes in longan, whole-genome scanning was used to identify the genes which contain the particular domain by both the hidden Markov model (HMM) and Blastn search methods. In total, 59 candidate WRKY genes were identified (Table S1). After the WRKY domain scanning and sequence alignment, three genes (*Dlo\_*007676.1, *Dlo\_*032703.1, and *Dlo\_*028398.1) without a complete predicted WRKY domain and one redundant gene (*Dlo\_*037584.1) were removed. Finally, 55 *DlWRKY* genes were determined in the longan genome (Table 1). According to their chromosome locations, the 55 *DlWRKY* genes were designated *DlWRKY*1*–DlWRKY*55. In addition, the basic properties of *DlWRKY* genes, including the length of the full-length sequence, open reading frame (ORF), protein sequence, molecular weight (MW), and PI, were systematically evaluated (Table 1). The average length of these *DlWRKY* genes was 2417 bp and the length mainly centered on the range of 892 bp (*DlWRKY*12) to 5385 bp (*DlWRKY*36). Meanwhile, the length of the ORF was mainly distributed from 480 bp (*DlWRKY*12 and *DlWRKY*34) to 3813 bp (*DlWRKY*36), with an average of 1237 bp. The length of the protein sequences ranged from 160 AA (*DlWRKY*12 and *DlWRKY*34) to 1271 AA (*DlWRKY*36), with an average of 411 AA. The protein MW ranged from 18.10 kDa (*DlWRKY*34) to 143.77 kDa (*DlWRKY*36), with an average of 44.73 kDa. The predicted isoelectric point of the DlWRKY proteins varied from 4.62 (*DlWRKY*22) to 9.77 (*DlWRKY*13), with an average of 7.11.


**Table 1.** The information of the *DlWRKY* gene family.


**Table 1.** *Cont.*
