**4. Approaches for Experimental Verification of the Systemic Experimental Data and Theoretical Predictions of Intrinsic Features of Plant mRNAs Important for Differential Translation**

As a rule, the experimental results on determination of the pools of differentially-translated plant mRNAs as well as the predictions of the regions and nucleotide contexts in differentially-translated transcripts require experimental confirmation of their functionality and contribution to the translation efficiency. In this section, we will consider the main experimental approaches that allow for convincing confirmation of which particular regions in individual mRNAs and/or features within plant transcripts are important for modulation of translation. It should be emphasized that methods for high-throughput experimental verification, i.e., concurrent analysis of a large pool of regulatory regions, are yet not available; thus, potential regulatory sequences are examined for each individual transcript [4].
