*4.3. Analysis of Protein Features and Chromosomal Locations*

The Mw and pI of each TCP protein were obtained using the online ExPASy program (http:// web.expasy.org/compute\_pi/). Protein pI was calculated using pK values of amino acids. Protein Mw was calculated by the addition of average isotopic masses of amino acids in the protein and the average isotopic mass of one water molecule. The subcellular localization of each GhTCP protein was analyzed using the CELLO v2.5 server (http://cello.life.nctu.edu.tw/). Through BLASTN (http: //cgp.genomics.org.cn/) searches against the *G. hirsutum* whole genome, some information was obtained about the physical locations of each *GhTCP* genes on chromosomes.
