*2.8. Statistics*

Data were analyzed using GraphPad Prism 8 software (GraphPad Software, Inc., La Jolla, CA, USA). The ROUT test was used to detect and remove any outliers. Normality and variance were tested before choosing parametric or non-parametric tests. To detect differences in liver qPCR and BA analysis, two-way ANOVA was used followed by Benjamini–Hochberg post hoc test with FDR adjustment, q < 0.05 was considered significant. Non-parametric BA data were analyzed by Kruskal–Wallis test followed Benjamini– Hochberg post hoc test with FDR adjustment. For organoid experiments, one-way ANOVA was performed followed by Tukey post hoc test, and the significance level was *p* < 0.05.
