*2.2. Drug Preparation*

CBD extracted from Cannabis plants was provided by Curaleaf International, UK, as a purified powder. Purity for CBD was above 95%; HPLC traces are provided as CBD powder was dissolved in a vehicle of Tween-80 (2%) and saline (NaCl 0.9%), protected from light, in agitation at 60 ◦C (avoiding boiling) until the complete mixing. The dissolved CBD was freshly prepared immediately prior to injection, and it was administered at different concentrations: 5 mg/kg, 15 mg/kg, and 30 mg/kg.
