3.3.6. Water T-Maze

A mixed design three-way ANOVA (shock × virus × test; 2 × 2 × 2) on the WTM experiment revealed significant main effects of shock [F(1,30) = 25.88, *p* < 0.001] and virus [F(1,30) = 23.67, *p* < 0.001]; with two significant interactions: shock × virus [F(1,30) = 28.735, *p* < 0.001] and test × shock × virus [F(1,30) = 7.50, *p* = 0.01] (Figure 4e). Post hoc comparisons revealed that the Shock/GFP group showed a significant increase in the number of trials required to reach the criterion in the acquisition and reversal phases compared with the NoShock/GFP group (acquisition, *p* < 0.01; reversal, *p* < 0.001) and Shock/OE group (acquisition and reversal, both *p* < 0.001). Hence, overexpression of β-catenin in the NAc prevented the shock- and reminders-induced impairment in performance in the WTM task.

#### 3.3.7. Forced Swim Test

Two-way ANOVA for the FST experiment revealed significant main effects on immobility of shock [F(1,30) = 78.646, *p* < 0.001] and virus [F(1,30) = 78.695, *p* < 0.001], with a significant shock × virus interaction [F(1,30) = 98.321, *p* < 0.01] (Figure 4f). Post hoc analysis revealed an increase in immobility in the Shock/GFP group compared with the NoShock/GFP and Shock/OE (both *p* < 0.001) groups, suggesting that overexpression of β-catenin in the NAc prevented the shock- and reminders-induced impairment in the FST.

No significant differences were observed between the NoShock/GFP and the NoShock/ OE groups in any of the behavioral measures, suggesting that the virus had no effect in non-shocked control rats.

#### *3.4. β-Catenin, mGluR5, and CB1 Receptors Regulation by Overexpressing NAc β-Catenin in Rats Exposed to Shock and Reminders*
