*2.10. Statistical Analysis*

Data from in vitro studies are represented as mean ± SE. The mean was calculated from the indicated number of experiments. The mean of triplicates from each experiment was used for this calculation. For statistical analysis of the macrophage NO• secretion experiments in female and male mice, we used Friedman test. For statistical analysis of the macrophage NO• secretion and cannabinoid receptors expression, macrophage cytokine secretion, and flow cytometry experiments in young and aged mice, we used the Mann–Whitney test. For statistical analysis of colon length, colon histopathology, macrophage infiltration to the colon, and blood cytokines in the DSS model experiments, we used the Kruskal–Wallis test. For statistical analysis of weight loss and clinical score in the DSS model experiments, we used the Kruskal–Wallis test of area under the curve (AUC). In all experiments, *p* value < 0.05 were considered statistically significant.
