GFP detection:

Brains were sectioned in 35-μm-thick slices using cryostat microtome (Leica Biosystems, Deer Park, IL, USA) and stored at 4 ◦C in PBS. Then, slices were washed three times for 15 min each in 1 × PBS (Sigma-Aldrich, St. Louis, MO, USA). After the washing procedure, the brain slices were mounted on super frost glass slides using PBS as a mounting solution and left to dry for 24 h. Glass slides were then stored at 4 ◦C in a dark chamber. Staining was documented using a confocal microscope at 5×, 10×, and 40× zoom (ZEISS, Jena, Germany).
