*4.4. Analysis of Evolutional Relationships of NRAMP Genes in A. catechu, O. sativa, and A. thaliana*

The sequences of AtNRAMPs and OsNRAMPs were obtained from TAIR (https: //www.arabidopsis.org/) (accessed on 12 January 2023) and the Rice Annotation Project Database, (https://rapdb.dna.affrc.go.jp/download/irgsp1.html) (accessed on 12 January 2023), respectively. ClustalW was performed to align all sequences with default parameters, and then a phylogenetic tree was constructed using a neighbor-joining (NJ) method of MEGA-X [45]. The bootstrap replications were set as 1000 to test the NJ tree reliability. The Evolview8 program was employed to visualize the phylogenetic tree [46].
