*4.3. Cell Viability*

RAW 264.7 cells were plated in a 48-well plate at a density of 3 × <sup>10</sup><sup>4</sup> cells/well. After 24 h, RAW 264.7 cells were pre-treated with 50, 100, and 150 μM of wistin for 30 min, followed by LPS (0.1 μg/mL) treatment and incubation for 24 h. After that, the DMEM medium was removed, and 1mg/mL of MTT solution was added to each well and was further incubated for 2 h at 37 ◦C. The solution was removed from the wells and the formed formazan crystals were dissolved in 200 μL of DMSO. Finally, absorbance was measured at 570 nm using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific Co., Waltham, MA, USA).
