2.1.5. Effect of CLL on Kidney and Liver Functions

Kidney function indicators (creatinine, urea, and uric acid) as well as plasma transaminases (AST and ALT) revealed significant elevation in obese rats compared to normal rats, in agreement with [25]. Treatment with Orly and CLL significantly improved kidney and liver functions, except for the significant elevation of uric acid content in the case of CLL, which is most probably attributed to the high oxalate level in the leaves [26] (Figure 1F,H, Table 1). Hence, CLL was significantly better than Orly in terms of kidneyand liver-function improvement, except for an elevated uric acid level (*p* < 0.05).

Overall, despite the better effect of Orly in reducing body weight gain compared to CLL, the latter revealed better improvement in mostly all tested biochemical parameters, except for an elevated uric acid level.

#### *2.2. Isolation and Structure Elucidation*

To identify anti-obese agents in the CLL extract, the extract was subjected to fractionation using column chromatography (CC) and liquid chromatography (LC), to afford 14 compounds (C1–C12) including 4 flavone glycosides, i.e., **1** (Figures S1–S6), **2** (Figures S7–S11), **3** (Figures S12–S14), and **4** (Figures S15–S19) as well as 10 dihydrochalcone glycosides, i.e., **5** (Figures S20–S24), **6** (Figures S25–S29), **7** (Figures S30–S36), **8** (Figures S37–S43), **9** (Figures S44–S48), **10a** and **10b** (Figures S49–S54), **11a** and **11b** (Figures S55–S60), and **12** (Figures S61–S67). All compounds were checked for their purity using HPLC (Figure S68).
