**4. Conclusions**

In the present study, nine extracts of *C. hypocistis* obtained using traditional solvents and NADESs were investigated for their total polyphenolic contents, antioxidant and enzyme inhibitory properties. The extracts were found to be richer in TPC than TFC. In particular, the NADES extracts were found to yield higher TPC compared to the other extracts. On the other hand, generally, ethanol/water, ethanol, and water showed very potent and better antioxidant potential compared to the other extracts, whereas hexane and dichloromethane exhibited weaker antioxidant potential in almost all antioxidant assays. While NADES-A extract displayed the highest anti-AChE activity, none of the NADES extracts displayed inhibition against the butylcholinesterase. With the exception of the water extract, all the other traditional solvent extracts showed dual cholinesterase inhibitory properties. Remarkably, the NADES extracts were found to have an enhanced antityrosinase effect compared to the traditional solvent extracts. Similarly, the NADES extracts were found to be better glucosidase inhibitors, and the NADES-B and -C extracts showed higher antiamylase activity in comparison with the other studied extracts. It is worth highlighting that this study demonstrated NADESs to have brought some improved abilities in bioactive content yields and bioactivity compared to the traditional extracts, especially in terms of TPC and some of the enzyme inhibitory activities. However, the traditional solvents were much better in extracting the antioxidant compounds. Hence, the current investigation enabled a comparison between traditional solvents and NADESs and suggested the potential of NADESs as an alternative to traditional organic solvents for higher extraction of phytonutrients and some better biological performance, although the traditional solvents were found to be more effective in yielding higher antioxidant activity. The paper proved the importance of natural medicine where sources of pro-health components are taken from plants. NADESs as mixtures obtained from compounds of natural origin, in respect to the studied examples, are nontoxic; thus, their extracts could be used as components of functional foods as well as food additives without the need of NADES removal.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules27185788/s1, Table S1: Total phenolic (TPC) and flavonoid content (TFC) of the tested extracts; Table S2: Proposed annotated compounds by UPLC-ESI-QTOF-MS in all Cytinus extracts; Table S3: Compound areas extracted for each Cytinus extract; Table S4: Antioxidant properties of the tested extracts; Table S5: Enzyme inhibitory of the tested extracts.

**Author Contributions:** Conceptualisation, G.Z., M.d.l.L.C.-G. and G.B.; methodology, G.Z., M.d.l.L.C.- G., Á.F.-O., F.J.L.-J., M.M., E.Y., R.K. and G.B.; software, G.Z., M.d.l.L.C.-G. and G.B.; validation, R.K., S.J. and M.F.M.; formal analysis, G.Z.; investigation, G.Z., S.J. and M.F.M.; resources, G.Z., E.Y. and R.K.; data curation, G.Z., M.d.l.L.C.-G. and M.F.M.; writing—original draft preparation, G.Z., M.d.l.L.C.-G., S.J. and M.F.M.; writing—review and editing, M.M. and G.B.; visualisation, G.Z.; supervision, G.B., A.S.C.; project administration, G.Z. and G.B.; funding acquisition, G.Z. and A.S.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** M. Momotko and G. Boczkaj gratefully acknowledge the financial support from the National Science Centre, Warsaw, Poland—decision no. UMO-2018/30/E/ST8/00642.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** The authors M.d.l.L.C.-G. and Á.F.-O. would like to thank the Regional Ministry of Economy, Knowledge, Enterprise and Universities of Andalusia for the contract for Young Researchers (PAIDI) at the University of Granada. F.J.L.-J. thanks the Spanish Ministry of Science and Innovation for the postdoctoral contract Juan de la Cierva-Formación (FJC2020-044298-I).

**Conflicts of Interest:** The authors declare no conflict of interest.
