*3.7. Statistical Analysis*

Statistical analyses were performed using GraphPad Prism version 5.01 software (San Diego, CA, USA). Means ± S.E.M. were determined for each experimental group and analyzed by one-way analysis of variance (ANOVA), followed by Newman–Keuls comparison multiple test. Statistical significance was set at *p* < 0.05. The number of animals randomized for each experimental group was calculated on the basis of the "resource equation" N = (E + T)/T (10 ≤ E ≤ 20) [38].

#### *3.8. Color Analysis*

The samples under examination (olive oil as vehicle present at about 46% in the formulation, and PollenAid Plus Fill as formulated product used to fill the capsules) were subjected to colorimetric analysis using a spectrometer X-Rite, equipped with full-spectrum D65 illuminant and an observer angle and an observer angle of 10◦. Cylindrical coordinates C\*ab and hab were calculated from a\* and b\* as is customary in the literature [15].
