*3.3. Cell Culture and Treatment*

Murine macrophage RAW 264.7 cells obtained from American Type Culture Collection (ATCC, #TIB-71; Rockville, MD, USA) were cultured in DMEM plus 10% FBS and 1% P/S and incubated under a temperature 37 ◦C in a humidified atmosphere of 5% CO2 incubator as previously described [29]. The cells were grown in culture dishes. After the confluence of cells reached 80%, the cell density was calculated and used for the subsequent experiment. The cells were passaged by trypsinization every 3–5 days.
