*2.7. Cell Culture*

P19 cells (ATCC CRL-1857) were grown in alpha minimal essential medium (α-MEM) supplemented with 7.5% newborn calf serum (NCS), 2.5% fetal bovine serum (FBS), and 1% antibiotics-antimycotic solution in a 5% CO2 humidified atmosphere, at 37 ◦C Cells in monolayer cultures were maintained in exponential growth by subculturing every 2 days [17].
