2.10.2. Co-Administration of H2O2 Assay

The DMSO solution of the extracts diluted with P19SM plus 10 μM Ara-C was added to give the final concentration of the extract at a concentration that enhanced survival of cultured neurons more than control. The 0.5% *v/v* DMSO in P19SM was used as control. The 5 mM H2O2 in P19SM plus 10 μM Ara-C was used to make oxidative stress condition. The 5 mM H2O2 and the extracts in P19SM plus 10 μM Ara-C were added together for coadministration assay. The cells were incubated for 18 h at 37 ◦C. Cell viability was assayed by XTT reduction method. The data were expressed as the average % cell viability ± SE (*n* = 3) [9]. Quercetin at concentration of 1 nM was used as positive control [26].
