*3.4. Plant Material and Preparation of Extracts*

*Cytinus hypocistis* samples were collected at the flowering season in June 2020 (Anamur, Mersin, Turkey). The plants were identified by one of the authors (Dr. Evren Yildiztugay) and voucher specimens were deposited at the herbarium of Selcuk University, Konya, Turkey. The samples' aerial parts were dried in the shade at room temperature for about 7 days, and then ground into a powder using a mill. All of the samples were kept in a dark place.

In this study, the extracts were prepared using traditional solvents (n-hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water (70%), and water) and NADEs. Maceration was employed as the extraction method to obtain n-hexane, ethyl acetate, dichloromethane, EtOH, and EtOH/water extracts. The plant materials (10 g) were macerated overnight at room temperature with 200 mL of these solvents. Finally, the solvents were evaporated from the mixtures. To obtain water extracts, the plant materials (10 g) were kept with 200 mL of boiled water, and then the extracts were filtered and lyophilised. In the preparation of NADE extracts, the plant materials (10 g) were mixed with the NADESs for 20 min at 25 ◦C in an ultrasonic bath. The extracts were filtered and all extracts were stored at 4 ◦C until further analysis was required.

#### *3.5. Chemical Reagents*

All chemicals were of HPLC-MS grade and used as received. Acetic acid and acetonitrile for UPLC were purchased from Fluka (Sigma-Aldrich, Steinheim, Germany) and Lab-Scan (Gliwice, Sowinskiego, Poland), respectively. For solutions, ultrapure water was obtained with a Milli-Q system Millipore (Bedford, MA, USA), and absolute ethanol was purchased from VWR chemicals (Radnor, PA, USA).
