*3.5. Co-Administration of H2O2 Assay*

P19-derived neurons culture was co-treated with 10 ng/mL of *M. pruriens* seed aqueous extract and 5 mM H2O2. The cell viability was compared with the toxic condition (5 mM H2O2). The cell viability was assayed by XTT reduction method. The results showed significant cell viability in 10 ng/mL *M. pruriens* seed extract co-treated with 5 mM H2O2 (25.01 ± 2.66%), compared with the toxic condition (11.61 ± 0.50%) (Figure 3). The % neuron viability when co-treated with 1 nM quercetin (positive control) and 5 mM H2O2 was 20.96 ± 3.67%. No significant difference was found between the % neuron viability when treated with 10 ng/mL *M. pruriens* seed extract and 1 nM quercetin.

**Figure 2.** P19-derived neurons cell culture viability in 10 ng/mL *Mucuna pruriens* seed aqueous extract compared with the positive control (1 nM quercetin) and toxic condition (0.5% *v/v* DMSO in α-MEM without serum). \* *p* < 0.05 compare with toxic condition (0.5% *v/v* DMSO in α-MEM without serum).

**Figure 3.** P19-derived neurons cell culture viability in 10 ng/mL *M. pruriens* seed aqueous extract cotreated with 5 mM hydrogen peroxide compared with the positive control (1 nM quercetin co-treated with 5 mM hydrogen peroxide) and toxic condition (5 mM hydrogen peroxide). \* *p* < 0.05 compared with toxic condition (5 mM hydrogen peroxide).
