*2.1. Effects of Wistin on Cell Viability in LPS-Induced RAW 264.7 Cells*

Because isoflavones have been reported to be cytotoxic at high doses, we attempted to confirm whether wistin is cytotoxic at high concentrations [35]. The cytotoxicity of wistin (Figure 1a) on the viability of RAW 264.7 cells was evaluated using the 3-(4, 5 dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (MTT) assay. Cotreatment of wistin (50 μM, 100 μM, and 150 μM) and LPS (0.1 μg/mL) did not show cytotoxicity in RAW 264.7 at 24 h (Figure 1b).

**Figure 1.** The effects of wistin on cell viability in LPS-induced RAW 264.7. (**a**) The chemical structure of wistin. (**b**) The cells were treated with the indicated concentrations of wistin (0, 50, 100, 150 μM) for 30 min prior to treatment with LPS (0.1 μg/mL) for 24 h, and then cell viability was examined using MTT assay. The data are presented as the means ± SD; *n* = 3.
