**5. Conclusions**

Taken together, our data show that Gemini-Cur comprehensively modulates gene expression in colorectal cancer HCT-116 cells. Gene ontology annotations related to DEGs found here include DNA-dependent ATPase (MF) during metabolic processes (BP) in nucleus (CC). Further analysis also demonstrated that Gemini-Cur dominantly affects cell cycle-related pathways. These RNA sequencing data greatly expand our understanding of the molecular and cellular targets of curcumin in cancer. We also reported novel potential targets for curcumin as listed in the top ten DEGs. Further investigations on the top up/downregulated genes especially in different cancer cell lines and non-cancerous controls will facilitate the findings of curcumin targets in colon cancer.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules27113470/s1, supplementary materials: Transcriptome Profiling of HCT-116 Colorectal Cancer Cells with RNA Sequencing Reveals Novel Targets for Polyphenol Nano Curcumin. The standard Bioconductor RNA-seq workflow (DESeq2), was employed to detect differentially expressed genes (DEGs) in Gemini-Cur treated cell compared to controls. Regarding the parameters *p* values and Log2 fold change, 3892 DEGs were selected as listed.

**Author Contributions:** Conceptualization, H.J.A. and E.B.; methodology, E.B. and F.N.; software, E.B., M.A.H.F. and E.B.; investigation, H.J.A.; writing—original draft preparation, E.B. and H.J.A.; writing—review and editing, F.N., E.B. and M.A.H.F.; supervision, E.B. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Data are contained within the article.

**Acknowledgments:** The authors would like to thank the staff of Genomics & Molecular biology Lab at the University of Tabriz for their technical assistance.

**Conflicts of Interest:** The authors declare no conflict of interest.

**Sample Availability:** Samples of the compounds are available from the authors.

#### **References**

