**4. Materials and Methods**

#### *4.1. Reagents*

All reagents were acquired from Sigma-Aldrich (St. Louis, MO, USA) except for HPLC grade methanol and dimethyl sulfoxide (DMSO), that were purchased from Panreac (Barcelona, Spain). Molecular probes were obtained from Invitrogen-Thermo Fisher Scientific (Carlsbad, CA, USA).
