*3.1. Plant Material*

The plant was harvested in the region of Khenifra (El Hammam) in late June 2019. It was cultivated in 2009 by the cooperative El Hammam for the valuation of Medicinal and Aromatic Plants, and it was not chemically treated. The site is located in the Middle Atlas of Morocco at a height of 1182 m. Identification of the species was confirmed at the Scientific Institute of Rabat. The collected samples were dried for ten days at room temperature in the shade.

#### *3.2. Phytochemical Screening*

Phytochemical screening is a qualitative study based on coloring and/or precipitation reactions from different plant extracts. It aims to highlight the important families of secondary metabolites contained in the plant. These extracts were obtained by decoction, infusion or maceration by solvents. Phytochemical screening is also based on the use of

several reagents. Table 4 contains the reagents and names of reactions used to recognize different groups.


**Table 4.** Reagents used in the search for secondary metabolites families.
