*4.9. Antioxidant Enzymatic Activity*

#### 4.9.1. SOD Enzymatic Activity

Superoxide dismutase catalyzes the conversion of superoxide anions into oxygen and hydrogen peroxide. In 96-well plates, it was added to the reaction mixture consisting of total cellular extracts, EDTA, buffer phosphate (pH 7.8, with 0.2% Triton X-100), hydroxylamine chlorohydrate, and nitroblue tetrazolium (NBT). NBT reduction was measured at 530 nm each minute, during 15 min, using a SPECTROstar Omega microplate reader (BMG Labtech, Ortenberg, Germany) [47].
