*2.2. Morphological Visualization of Apoptosis*

To visualize morphological changes in MDA-MB-231 cells, Hoechst staining was performed. As Figure 3 illustrates, TNBC cells undergo apoptosis after treatment with 20 μM Gemini-Cur and 13 μM cisplatin in both singular and combination forms. The microscopic visualization shows a uniformly light stain on the cells. However, a remarkable change in color intensity is clearly seen in treated cells with a significant difference in the number of apoptotic cells in Gemini-Cur/Cis treated group. The Hoechst staining clearly differentiate cells with nuclear fragmentation and DNA condensation as specified by arrows.
