4.5.3. Giemsa Staining

Cells were colored with the Giemsa stain. Briefly, 100 μL of pure ethanol were added to a 96-well plate containing the treated cells after removing the nutrient medium. Cells were left in ethanol for five minutes and then 100 μL of the Giemsa stain were added. Plates remained for 15 min at room temperature, followed by stain removal and cell washing with 100 μL of NaCl 0.9% (*v*/*v*). Cells were microscopically observed at 24, 48 and 72 h of incubation.
