*3.3. Extraction Procedure*

One hundred milligrams of different powdered plant material of the three studied species was extracted, in a 50 mL volumetric flask, with 10 mL of ethanol:water, 96:4 (*v:v*), followed by sonication (60 W, 25 ◦C, 37 Hz) for 20 min. The obtained extracts were centrifugated for 10 min under 3000 rpm and filtered using Whatman filter paper (Merck Life Science, Merck KGaA, Darmstadt, Germany). The extraction procedure was repeated three times, and then the filtrates were combined, evaporated to dryness by a rotavapor and stored at 4 ◦C. The yields of the extracts, referring to 100 g of dried plant material, were 31.37% for *E. multiflora* leaves (*Em*-L), 33.26% for *E. multiflora* aerial parts (*Em*-A), 37.97% for *E. scoparia* leaves (*Es*-L), 46.76% for *E. scoparia* aerial parts (*Es*-A), and 33.72% for *C. vulgaris* leaves (*Cv*-L).
