*4.4. LC/Q-TOF/HRMS Conditions*

To identify the chemical profile of *R. officinalis* extract an HPLC system (high performance liquid chromatography) consisting of a degasser, autosampler, quaternary pump, diode array detector, and column oven (Agilent Series 1260, Agilent Technologies, Santa Clara, CA, USA), coupled to a 6530 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) was used. Experimental conditions were adjusted as in the study of Kakouri et al., (2019) [70]. The extract was analyzed under the positive and negative ionization mode. The parameters set for the Q-TOF mass analysis follow those described at our previous analysis [70]. CID-ms/ms spectra were recorded on the auto MS/MS mode. Mass range was set to 50–1000 and collision energy was set at 40 V. Results were analyzed using the Agilent MassHunter Workstation software LC-MS Data Acquisition for 6530 series Q-TOF (version B07.00, Agilent Technologies, Santa Clara, CA, USA).

#### *4.5. Evaluation of Cytotoxicity*
