*3.1. Plant Material and Extract Preparation*

Plant material of *Alchemilla viridiflora* Rothm., Rosaceaeand the preparation of the methanol extract was previously described by Radovic et al. (2022), and the same extract was used to conduct the current study [16]. Briefly, *A. viridiflora***,** aerial flowering parts were collected on carbonate soil in subalpine pastures at 1750 m s.m. on Mount Suva Planina in July 2019. Dr. Marjan Niketic identified plant material from voucher specimens (20130708/1-2) that were placed in the Natural History Museum (Belgrade, Serbia). The dry extract was obtained by methanol extraction for two days after which the solvent was evaporated under low pressure. The yield of dry methanol extract from powdered plant material was 28.3%.
