3.4.3. Standards Employed

Calibration curves of three polyphenolic standards (kaempferol-3-glucoside, apigenin, rosmarinic acid) were employed for the quantification of the polyphenolic content in sample extracts. Each analysis was performed in 6 repetitions. Data acquisition was performed by Shimadzu LabSolution software ver. 5.99. Kaempferol-3-glucoside (1, 10, 20, 50, 150) ppm; y = 13848x + 2354.1; R2 = 0.9995; LoD = 0.090; LoQ = 0.274. Apigenin (1, 10, 25, 50, 100) ppm; y = 25625x + 608.11; R2 = 0.9997; LoD = 0.0263; LoQ = 0.0798. Rosmarinic acid (5, 10, 25, 50, 100) ppm; y = 7150.6x + 9821.3; R<sup>2</sup> = 0.9993; LoD = 0.14; LoQ = 0.43.
