Targeting Asparagine and Serine Metabolism in Germinal Centre-Derived B Cells Non-Hodgkin Lymphomas (B-NHL)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Preparation of Customised Complete RPMI-1640 Medium
2.3. Isotope Labelling
2.4. Cell Viability Assay
2.5. Western Blotting
2.6. NMR Sample Preparation
2.7. NMR Data Acquisition
2.8. Analysis of NMR Spectra
2.9. Transcriptomic Data and RNA-Seq Data Analysis
3. Results
3.1. BL Cells Consumed Dramatically More Extracellular Asparagine Than DLBCL and Regulates Serine Metabolism
3.2. BL Tumours Differentially Express the Genes Involved in Serine Metabolism Compared to DLBCL
3.3. Asparaginase and NCT503 Reduce Extracellular Asparagine and Serine Production from Glucose Respectively
3.4. Combination of ASNase with NCT Increases the Sensitivity of BL Cells to ASNase
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Eraslan, Z.; Papatzikas, G.; Cazier, J.-B.; Khanim, F.L.; Günther, U.L. Targeting Asparagine and Serine Metabolism in Germinal Centre-Derived B Cells Non-Hodgkin Lymphomas (B-NHL). Cells 2021, 10, 2589. https://doi.org/10.3390/cells10102589
Eraslan Z, Papatzikas G, Cazier J-B, Khanim FL, Günther UL. Targeting Asparagine and Serine Metabolism in Germinal Centre-Derived B Cells Non-Hodgkin Lymphomas (B-NHL). Cells. 2021; 10(10):2589. https://doi.org/10.3390/cells10102589
Chicago/Turabian StyleEraslan, Zuhal, Grigorios Papatzikas, Jean-Baptiste Cazier, Farhat L. Khanim, and Ulrich L. Günther. 2021. "Targeting Asparagine and Serine Metabolism in Germinal Centre-Derived B Cells Non-Hodgkin Lymphomas (B-NHL)" Cells 10, no. 10: 2589. https://doi.org/10.3390/cells10102589