Search Results (412)

Grape pomace (GP), a significant by-product of winemaking, is gaining increasing recognition for its potential as a source of bioactive compounds with antioxidant and cardioprotective properties. This study aimed to characterize the polyphenolic profile, fatty acid composition, and antioxidant activity of 17 GP samples from Transylvanian cultivars. Polyphenolic content was determined using the Folin–Ciocalteu method and high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC–DAD–ESI MS) analysis. Fatty acid composition was analyzed using gas chromatography with flame ionization detection (GC–FID). Antioxidant capacity was assessed using five methods, which included the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2′-azino-bis (3-ethylbenzothialzoline-6-sulfonic acid) (ABTS) radical scavenging, ferric-reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC), and reducing power (RP) assays. Additionally, all extracts were analyzed by Fourier transform infrared (FTIR) spectroscopy to identify the presence of functional groups and chemical bonds associated with bioactive compounds. The results showed that Neuburger (NE), Radames (RA), and Regent (RE) cultivars had the highest phenolic concentrations, particularly of catechin, epicatechin, and procyanidin dimers. NE and Feteascǎ Regalǎ (FR) exhibited the greatest radical scavenging and electron transfer activities across multiple antioxidant assays. Rose Blaj (RB) and Astra (AS) displayed the most favorable fatty acid profiles, with high unsaturated-to-saturated fatty acid (UFA/SFA) and hypocholesterolemic-to-hypercholesterolemic fatty acid (H/H) ratios, as well as low atherogenicity (AI) and thrombogenicity (TI) indices, suggesting cardioprotective potential. Additionally, RB and NE cultivars also demonstrated a strong chelation of Cu²⁺ and Fe²⁺ ions, enhancing their antioxidant efficacy by mitigating metal-catalyzed oxidative stress. These findings underscore the potential of GP, particularly from NE, RB, RA, and AS cultivars, the last three of which were homologated in Transylvania at SCDVV Blaj, as valuable sources of health-promoting compounds for use in food, nutraceuticals, and other health-related applications. Full article

Background: Although the safety of COVID-19 vaccines has been demonstrated in clinical trials, real-world pharmacovigilance remains essential to detect rare or unexpected adverse events following immunization (AEFI). In Mexico, the national AEFI surveillance system is in place, yet there is limited analysis of state-level data. Objective: To characterize AEFI related to five COVID-19 vaccines and identify factors associated with AEFI type and seriousness in Nuevo León, Mexico. Methods: A retrospective analysis of the State of Nuevo León AEFI database was conducted, including all AEFI reports between December 2020 and June 2022 (n = 2213). Data included patient sex, age, vaccine type (Pfizer/BioNTech, AstraZeneca, Sinovac, Moderna, CanSino), number of doses (1 or ≥2), symptom categories, and AEFI seriousness. Symptoms were classified as local or systemic and grouped by organ systems. Descriptive analysis and binary multivariate logistic regression were used to examine associations between demographic and vaccine-related factors with AEFI type and severity. Odds ratios (OR) with 95% confidence intervals (CI) were estimated. Results: Most AEFI reports involved females aged 19–59 years and occurred after the first vaccine dose. The most frequently reported unexpected adverse events (UAEs) were mild to moderate, including injection-site reactions, headache, chills, fatigue, nausea, fever, dizziness, weakness, myalgia, and tachycardia. The Pfizer/BioNTech vaccine was associated with higher odds of arm pain and lower odds of hemorrhagic events. Receiving ≥2 doses increased the odds of arm pain and systemic symptoms. Less than 3% of AEFIs were classified as serious. Older adults (≥65 years) and second vaccine doses were associated with increased odds of a serious AEFI, while female sex and receiving the Pfizer/BioNTech vaccine were associated with reduced odds. Conclusions: In Nuevo León, most AEFIs related to COVID-19 vaccination were mild to moderate and resolved without complications. Serious AEFIs were uncommon, with older age and second doses associated with higher risk, and female sex and Pfizer/BioNTech vaccination associated with lower risk. These findings provide a local perspective on vaccine safety that complements national and international evidence. Full article

Background/Objectives: The COVID-19 pandemic was characterized by the rapid transmission of the virus and a global race for vaccines, with vaccines such as AstraZeneca, CoronaVac, Pfizer, and Janssen arriving in Brazil in 2020. Concurrently, an infodemic of information, driven by the media and social media, highlighted the importance of health communication. This study examines how online newspapers in a Brazilian state disseminated information about vaccination and its relationship with vaccine adherence among the population. Methods: Quantitative research, in which a total of 5308 journalistic articles were verified, using two databases, one for the publication of journalistic articles and the other for vaccinations in the state, which applied 9,577,567 doses in the period. Results: The analyses demonstrated a positive correlation between the number of publications of articles and the number of applications of vaccines (rho = 0.407, p-value < 0.0005), revealing a relationship of both increase and decrease in the publication of newspaper articles and the application of vaccines in specific weeks during the analysis period. Vaccination data revealed low adherence to the booster dose by the population, with unequal values among the cities of the state. Conclusions: The study highlighted the potential importance of newspapers in disseminating information about vaccines during the pandemic, underscoring the need for regional health strategies to increase vaccination coverage. Full article

Background: Durvalumab (anti-PD-L1) in combination with gemcitabine and cisplatin has become the first-line treatment for patients with locally advanced, surgically unresectable, or metastatic biliary tract cancer, following the survival benefit demonstrated in the TOPAZ-1 phase III trial. This study presents real-world data from UK centres in patients who received early access to the regimen via AstraZeneca’s scheme. The aim was to assess the safety and efficacy of this treatment approach in routine clinical practice and compare it to outcomes reported in the TOPAZ-1 trial. Method: This retrospective study included patients with locally advanced, surgically unresectable, or metastatic biliary tract adenocarcinoma who received durvalumab in combination with gemcitabine and cisplatin. Data were collected across ten UK centres. The primary endpoint was progression-free survival (PFS), with secondary endpoints including overall survival (OS), overall response rate (ORR), and safety outcomes, encompassing both chemotherapy and immunotherapy-related adverse events (AEs). Results: A total of 134 patients treated between April 2022 and December 2023 were included. The median follow-up was 12.8 months (95% CI: 11–16.8). The median PFS was 8.83 months (95% CI: 5.73–11.7), closely aligning with the 7.2 months reported in TOPAZ-1 (95% CI: 6.7–7.4). The median OS was 12 months (95% CI: 10.7–13.9), slightly below the 12.8 months observed in TOPAZ-1 (95% CI: 11.1–14.0). The ORR was 29.1% (TOPAZ-1: 26.7%), and the disease control rate was 61.2%. In terms of safety, 64 patients (52.3%) experienced any-grade AEs, and 9 patients (6.8%) had grade 3–4 AEs, representing a lower toxicity profile than TOPAZ-1. Immunotherapy-related AEs occurred in 25 patients (18.7%), with grade 3–4 events in 3%. Conclusions: These real-world findings from UK cancer centres support the outcomes of the TOPAZ-1 trial, demonstrating comparable efficacy and a favourable safety profile for durvalumab combined with gemcitabine-cisplatin as first-line treatment for advanced biliary tract cancer. Full article

Background/Objectives: The non-small-cell lung cancer (NSCLC) therapeutic landscape has undergone a profound transformation with the introduction of multiple personalized treatment options. Mutations in ERBB2 (HER2) have recently emerged as promising novel targets for the treatment of non-squamous NSCLC (nsNSCLC). Accurate, rapid, and efficient molecular profiling is crucial for identifying patients who may benefit from targeted therapies, including HER2-directed agents. Materials and Methods: Here, we aimed to retrospectively assess the performance of the Oncomine™ Precision Assay* (OPA) in combination with the Ion Torrent Genexus™ Integrated Sequencer* (Thermo Fisher Scientific. Waltham, MA, USA) for detecting ERBB2 mutations in nsNSCLC. A total of 108 archived nsNSCLC samples, consisting of biopsies, resections, and cytological specimens, were used to assess concordance with in-house-validated orthogonal tests. Results: The OPA showed high sensitivity and specificity with an overall accuracy of 100% for single-nucleotide variants (SNVs) and insertions and deletions (Indels). SNVs and Indels with allele frequencies as low as 5% were correctly identified across samples with a tumor cell content ranging from 5% to 95%. Additionally, the assay demonstrated high reproducibility across the six participating laboratories. The turnaround time of the OPA was notably shorter compared to traditional orthogonal methods, facilitating rapid molecular report generation. Conclusions: The OPA in combination with the Ion Torrent Genexus™ System allows for highly sensitive and specific detection of relevant ERBB2 mutations. The assay’s streamlined workflow, coupled with its automated data analysis pipeline, enables a fast turnaround time for testing across a range of sample types. This includes samples with reduced tumor cell content and limited available input. This study demonstrates the future potential of using this assay in a clinical setting. Full article

Rocket launches generate infrasound signatures that have been detected at great distances. Due to the sparsity of the networks that have made these detections, however, most signals are detected tens of minutes to hours after the rocket launch. In this work, a method of near-real-time detection of rocket launches using data from a network of smartphones located 10–70 km from launch sites is presented. A machine learning model is trained and tested on the open-access Aggregated Smartphone Timeseries of Rocket-generated Acoustics (ASTRA), Smartphone High-explosive Audio Recordings Dataset (SHAReD), and ESC-50 datasets, resulting in a final accuracy of 97% and a false positive rate of <1%. The performance and behavior of the model are summarized, and its suitability for persistent monitoring applications is discussed. Full article

The association between COVID-19 vaccination and thromboembolic events has garnered significant research attention, particularly with the advent of vaccines based on adenoviral vectors, including AstraZeneca’s and Johnson & Johnson’s vaccines. This review underscores the uncommon occurrence of venous thromboembolism (VTE), arterial thromboembolism (ATE), and vaccine-induced thrombotic thrombocytopenia (VITT) following COVID-19 vaccination. Although these complications are extremely rare compared to the heightened risk of thrombosis from COVID-19 infection, elements like age, biological sex, type of vaccine and underlying health conditions may contribute to their development. In addition, rare renal complications such as acute kidney injury and thrombotic microangiopathy have been documented, broadening the spectrum of potential vaccine-associated thrombotic manifestations. Current guidelines emphasize early detection, individualized risk assessment, and use of anticoagulation therapy to mitigate risks. Despite these events, the overwhelming majority of evidence supports the continued use of COVID-19 vaccines, given their proven efficacy in reducing severe illness and mortality. In addition, recent comparative data confirm that mRNA-based vaccines are associated with a significantly lower risk of serious thrombotic events compared to adenoviral vector platforms. Ongoing research is essential to further refine preventive and therapeutic strategies, particularly for at-risk populations. Full article

This study presents the design and development of electromagnetic dipole magnets for use as beam dumps and spectrometers in the MIR and THz free-electron laser (FEL) beamlines at the PBP-CMU Electron Linac Laboratory (PCELL). The magnets were optimized to achieve a 60-degree bending angle for electron beams with energies up to 30 MeV, without requiring water cooling. Using CST EM Studio for 3D magnetic field simulations and ASTRA for particle tracking, the THz dipole (with 414 turns) and MIR dipole (with 600 turns) generated magnetic fields of 0.1739 T and 0.2588 T, respectively, while both operating at currents below 10 A. Performance analysis confirmed effective beam deflection, with the THz dipole showing that it was capable of handling beam energies up to 20 MeV and the MIR dipole could handle up to 30 MeV. The energy measurement at the spectrometer screen position was simulated, taking into account transverse beam size, fringe fields, and space charge effects, using ASTRA. The energy resolution, defined as the ratio of energy uncertainty to the mean energy, was evaluated for selected cases. For beam energies of 16 MeV and 25 MeV, resolutions of 0.2% and 0.5% were achieved with transverse beam sizes of 1 mm and 4 mm, respectively. All evaluated cases maintained energy resolutions below 1%, confirming the spectrometer’s suitability for high-precision beam diagnostics. Furthermore, the relationship between the initial and measured energy spread errors, taking into account a camera resolution of 0.1 mm/pixel, was evaluated. Simulations across various beam energies (10–16 MeV for the THz dipole and 20–25 MeV for the MIR dipole) confirmed that the measurement error in energy spread decreases with smaller RMS transverse beam sizes. This trend was consistent across all tested energies and magnet configurations. To ensure accurate energy spread measurements, a small initial beam size is recommended. Specifically, for beams with a narrow initial energy spread, a transverse beam size below 1 mm is essential. Full article

The generation of high-quality mid-infrared free-electron laser (MIR-FEL) radiation depends critically on precise control of electron beam parameters, including energy, energy spread, transverse emittance, bunch charge, and bunch length. At the PBP-CMU Electron Linac Laboratory (PCELL), effective beam diagnostics are essential for optimizing FEL performance. However, dedicated systems for direct measurement of transverse emittance and bunch length at the undulator entrance have been lacking. This paper addresses this gap by presenting the design, simulation, and analysis of diagnostic stations for accurate characterization of these parameters. A two-quadrupole emittance measurement system was developed, enabling independent control of beam-focusing in both transverse planes. An analytical model was formulated specifically for this configuration to enhance emittance reconstruction accuracy. Systematic error analysis was conducted using ASTRA beam dynamics simulations, incorporating 3D field maps from CST Studio Suite and fully including space-charge effects. Results show that transverse emittance values as low as 0.15 mm·mrad can be measured with less than 20% error when the initial RMS beam size is under 2 mm. Additionally, quadrupole misalignment effects were quantified, showing that alignment within ±0.95 mm limits systematic errors to below 33.3%. For bunch length measurements, a transition radiation (TR) station coupled with a Michelson interferometer was designed. Spectral and interferometric simulations reveal that transverse beam size and beam splitter properties significantly affect measurement accuracy. A 6% error due to transverse size was identified, while Kapton beam splitters introduced additional systematic distortions. In contrast, a 6 mm-thick silicon beam splitter enabled accurate, correction-free measurements. The finite size of the radiator was also found to suppress low-frequency components, resulting in up to 10.6% underestimation of bunch length. This work provides a practical and comprehensive diagnostic framework that accounts for multiple error sources in both transverse emittance and bunch length measurements. These findings contribute valuable insight for the beam diagnostics community and support improved control of beam quality in MIR FEL systems. Full article

Foam–CO₂ EOR Flooding is not very successful if unaccompanied by foam stabilizers such as nanoparticle fly ash (NFA). This study was conducted to determine the effect of NFA on foam stability by considering particle characteristics using the Bulk Foam Method as an additional condition. The test resulted in half-life times, which showed that in the absence of NFA, when oil was added, it was 211.5 s, and in salinity conditions, it was 232.5 s. This succeeds in improving half-life times to 226 s (with oil) and 241.5 s (with salinity) by adding NFA-Type F. For further research, conducting tests using reservoir conditions is recommended. Full article

Background and Objectives: Community-acquired pneumonia (CAP) remains a major health burden worldwide, with high morbidity and mortality, particularly among older adults and those with comorbidities. This study aimed to evaluate the etiological factors of CAP and to investigate systemic inflammatory markers (IL-6, IL-8, IFN-γ, and G-CSF) in blood samples collected from CAP patients to identify which markers could be targets for potential etiological, clinical, and therapeutic interventions. Materials and Methods: A prospective study was conducted in 41 patients with confirmed CAP hospitalised during the winter season of 2024–2025. Clinical, demographic, and laboratory data were collected at admission and seven days later. Serum IL-6, IL-8, IFN-γ, and G-CSF concentrations were measured using a multiplex assay. Results: Aetiology was identified in 87.8% of cases, with typical bacterial pathogens being more prevalent among older, smoking patients, while atypical pathogens were more common among younger, non-smoking patients. Hospitalisation and increased inflammatory markers were associated with older age. After seven days of treatment, significant decreases in IL-6, IFN-γ, and G-CSF concentrations were observed. IFN-γ levels were significantly higher in patients with atypical aetiology. Higher concentrations of IL-8 and G-CSF were associated with hospitalisation. IL-6 levels were positively correlated with age, C-reactive protein (CRP), and pneumonia severity index (PSI) scores. Conclusions: Systemic inflammatory markers, especially IL-6, IL-8, IFN-γ, and G-CSF, may be valuable tools in managing generalised pneumonia. They can help to differentiate etiologically, assess disease severity, and make treatment decisions. Full article

Objectives: Shoulder injury related to vaccine administration (SIRVA), previously observed with influenza vaccines, has gained clinical significance with widespread COVID-19 vaccination. However, few studies correlate vaccine types and demographic factors with the MRI findings of SIRVA. This study aimed to evaluate MRI findings of SIRVA following COVID-19 vaccination and assess associations with vaccine type and patient characteristics. Methods: A retrospective cohort study was conducted on 35 patients with new-onset shoulder complaints within six weeks of COVID-19 vaccination between May 2021 and May 2022. MRI findings suggestive of SIRVA were reviewed, including subacromial bursitis, rotator cuff tears, and adhesive capsulitis. Demographic data, vaccine type, clinical symptoms, and treatments were collected. Follow-up interviews (1–30 September 2024) assessed symptom persistence and vaccine hesitancy. Descriptive statistics and Chi-square tests were used to explore associations. Results: Of the 35 patients (mean age 53.6 ± 9.0 years; 54.3% female), subacromial bursitis was the most common MRI finding (89.5%), followed by tendonitis (47.4%) and adhesive capsulitis (36.8%). Tendonitis correlated with older age (p = 0.024) and AstraZeneca vaccination (p = 0.033). Subacromial bursitis was linked to female sex (p = 0.013) and higher BMI (p = 0.023). Adhesive capsulitis was associated with receiving the Sinopharm vaccine (p = 0.029). Persistent symptoms (22.9%) were more common in younger patients, women, and those with right-sided injections. Conclusions: SIRVA following COVID-19 vaccination showed different MRI patterns associated with female sex, higher BMI, and vaccine type. Awareness of these patterns may expedite recognition of COVID-19-associated SIRVA in routine practice. Full article

Chronic rhinosinusitis (CRS) is a persistent inflammatory condition of the sinus mucosa characterized by significant tissue remodeling. This study aimed to evaluate the gene expression of extracellular matrix (ECM) proteins, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) in post-operative tissues of CRS patients. A total of 30 patients diagnosed with CRS, divided into CRSwNP (with nasal polyps) and CRSsNP (without nasal polyps) groups, were compared with a control group of 10 individuals undergoing nasal surgeries for non-CRS conditions. Gene expression analysis was conducted using quantitative real-time PCR, and plasma cytokine levels were measured via ELISA. Results indicated significantly higher expression of collagen I, collagen III, fibronectin, vimentin, periostin, and tenascin C in CRS tissues, especially in CRSsNP patients. Conversely, elastin expression was markedly lower. MMP-2, MMP-9, TIMP-1, and TIMP-2 expression was significantly altered, with CRSsNP showing lower levels compared to CRSwNP and controls. TGF-β1 expression was elevated in both CRS groups, particularly in CRSsNP, highlighting its role in fibrosis and ECM remodeling. Additionally, increased plasma concentrations of TSLP and TGF-β1 suggest epithelial activation and immune dysregulation in CRS. These findings underscore distinct remodeling profiles in CRS endotypes, emphasizing the need for targeted therapeutic strategies based on molecular phenotyping. Understanding ECM dysregulation and inflammatory pathways in CRS may lead to improved, individualized treatment approaches. Full article

The aim of the present study was to assess heavy metal tolerance and its accumulation potential in coastal nitrophilic species Rumex maritimus, and to study the possible effects of nitrogen fertilizer and salinity on the characteristics of metal-treated plants. Two experiments were performed in partially controlled greenhouse conditions: (1) gradual treatment with increasing concentrations of Cd, Pb, Cu, Mn, and Zn; and (2) acute treatment with Cd, Pb, and Cu on the background of different nitrogen fertilizer amendment rates (0.15 and 0.30 g L⁻¹ N) and salinity (50 and 100 mM). R. maritimus plants were extremely tolerant to treatment with all metals, with no negative effect on total leaf biomass both in the case of gradual or acute treatment. However, the number and biomass of dry leaves increased under high doses of heavy metals, and the effect was more pronounced in the case of acute treatment. All studied metals were excluded from the roots and young leaves, predominantly accumulating in the dry leaves, reaching 250 mg kg⁻¹ for Cd, 2000 mg kg⁻¹ for Pb, and 500 mg kg⁻¹ for Cu. In the second experiment, the presence of increased nitrogen in the substrate positively affected the growth of R. maritimus plants and their morphological response to heavy metals, but salinity affected metal accumulation. Photosynthesis-related parameters, leaf chlorophyll concentration, and the chlorophyll a fluorescence parameter Performance Index Total confirmed that heavy metals had no negative effect on the physiological state of photosynthetically active leaves. It is concluded that R. maritimus plants have exceptional potential for practical phytoremediation needs due to the high tolerance and accumulation potential for heavy metals. Full article

Vaccination has been instrumental in curbing the transmission of SARS-CoV-2 and mitigating the severity of clinical manifestations associated with COVID-19. Numerous COVID-19 vaccines have been developed to this effect, including BioNTech-Pfizer and Moderna’s mRNA vaccines, as well as adenovirus vector-based vaccines such as Oxford–AstraZeneca. However, the emergence of new variants and subvariants of SARS-CoV-2, characterized by enhanced transmissibility and immune evasion, poses significant challenges to the efficacy of current vaccination strategies. In this review, we aim to comprehensively outline the landscape of emerging SARS-CoV-2 variants of concern (VOCs) and sub-lineages that have recently surfaced in the post-pandemic years. We assess the effectiveness of existing vaccines, including their booster doses, against these emerging variants and subvariants, such as BA.2-derived sub-lineages, XBB sub-lineages, and BA.2.86 (Pirola). Furthermore, we discuss the latest advancements in vaccine technology, including multivalent and pan-coronavirus approaches, along with the development of several next-generation coronavirus vaccines, such as exosome-based, virus-like particle (VLP), mucosal, and nanomaterial-based vaccines. Finally, we highlight the key challenges and critical areas for future research to address the evolving threat of SARS-CoV-2 subvariants and to develop strategies for combating the emergence of new viral threats, thereby improving preparedness for future pandemics. Full article