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23 pages, 7551 KB  
Article
Development of Automatic Labels for Cold Front Detection in South America: A 2009 Case Study for Deep Learning Applications
by Dejanira Ferreira Braz, Luana Albertani Pampuch, Michelle Simões Reboita, Tercio Ambrizzi and Tristan Pryer
Climate 2025, 13(10), 211; https://doi.org/10.3390/cli13100211 - 8 Oct 2025
Abstract
Deep learning models for atmospheric pattern recognition require spatially consistent training labels that align precisely with input meteorological fields. This study introduces an automatic cold front detection method using the ERA5 reanalysis dataset from the European Centre for Medium-Range Weather Forecasts (ECMWF) at [...] Read more.
Deep learning models for atmospheric pattern recognition require spatially consistent training labels that align precisely with input meteorological fields. This study introduces an automatic cold front detection method using the ERA5 reanalysis dataset from the European Centre for Medium-Range Weather Forecasts (ECMWF) at 850 hPa, specifically designed to generate physically consistent labels for machine learning applications. The approach combines the Thermal Front Parameter (TFP) with temperature advection (AdvT), applying optimized thresholds (TFP < 5 × 10−11 K m−2; AdvT < −1 × 10−4 K s−1), morphological filtering, and polynomial smoothing. Comparison against 1426 manual charts from 2009 revealed systematic spatial displacement, with mean offsets of ~502 km. Although pixel-level overlap was low, with Intersection over Union (IoU) = 0.013 and Dice coefficient (Dice) = 0.034, spatial concordance exceeded 99%, confirming both methods identify the same synoptic systems. The automatic method detects 58% more fronts over the South Atlantic and 44% fewer over the Andes compared to manual charts. Seasonal variability shows maximum activity in austral winter (31.3%) and minimum in summer (20.1%). This is the first automatic front detection system calibrated for South America that maintains direct correspondence between training labels and reanalysis input fields, addressing the spatial misalignment problem that limits deep learning applications in atmospheric sciences. Full article
(This article belongs to the Special Issue Meteorological Forecasting and Modeling in Climatology)
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10 pages, 739 KB  
Article
SARS-COV-2 Vaccination Response in Non-Domestic Species Housed at the Toronto Zoo
by Sara Pagliarani, Jaime Tuling, Phuc H. Pham, Alexander Leacy, Pauline Delnatte, Brandon N. Lillie, Nicholas Masters, Jamie Sookhoo, Shawn Babiuk, Sarah K. Wootton and Leonardo Susta
Vaccines 2025, 13(10), 1037; https://doi.org/10.3390/vaccines13101037 - 8 Oct 2025
Abstract
Background: Due to the wide host range of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), vaccination has been recommended for susceptible species in zoological collections, particularly to protect endangered species. The Zoetis® Experimental Mink Coronavirus Vaccine (Subunit) was temporarily authorized [...] Read more.
Background: Due to the wide host range of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), vaccination has been recommended for susceptible species in zoological collections, particularly to protect endangered species. The Zoetis® Experimental Mink Coronavirus Vaccine (Subunit) was temporarily authorized in 2021–2024 for emergency use in North America for this purpose. However, there are limited data regarding its safety or efficacy in non-domestic mammals. The present study was conducted to assess the ability of this vaccine to elicit serum neutralizing titers against SARS-CoV-2 in selected animals from the Toronto Zoo (TZ) vaccinated during 2022. Methods: Serum samples were collected from 24 individuals across four families (Cervidae, Felidae, Ursidae, and Hyaenidae) and tested using a surrogate virus neutralization test (sVNT) and a plaque-reduction neutralization test (PRNT). Results: The results showed that all species developed some neutralizing titers after at least one vaccine dose, except for polar bears, which showed no seroconversion. Felids and hyenas had the highest neutralizing titers, which peaked at 3 and declined between 4 and 6 months after boost. These differences may stem from species-specific immune responses or lack of vaccination protocols tailored to individual species. Conclusions: While natural infection with SARS-CoV-2 could not be ruled out in the cohort of this study, insights from our results have the potential to inform future vaccine recommendations for non-domestic species. Furthermore, our study highlighted the value of competitive assays in assessing serological responses across a broad range of exotic species, for which reagents, such as anti-isotype antibodies, are often unavailable. Full article
(This article belongs to the Collection COVID-19 Vaccine Development and Vaccination)
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19 pages, 1413 KB  
Article
Trends, Collaborations and Perspectives in the Study of Organizational Climate and Job Satisfaction: A Bibliometric and Scientometric Analysis
by Ramón Rubio, Luis Araya-Castillo, Hugo Moraga-Flores and María Francisca Ortega Frei
Adm. Sci. 2025, 15(10), 389; https://doi.org/10.3390/admsci15100389 - 7 Oct 2025
Abstract
Organizational climate (OC) and job satisfaction (JS) are constructs that have been studied for more than five decades. However, the results to date are not sufficient to generalize conclusions across cultures, countries and sectors. To contribute to the development of theory and practice, [...] Read more.
Organizational climate (OC) and job satisfaction (JS) are constructs that have been studied for more than five decades. However, the results to date are not sufficient to generalize conclusions across cultures, countries and sectors. To contribute to the development of theory and practice, this study analyzes publication trends through a bibliographic review of publications indexed in the Web of Science (WoS) database between 1975 and 2023, using bibliometric and scientometric techniques. This review synthesizes the accumulated knowledge and reveals significant gaps that need to be addressed, highlighting the weak articulation of research in general; the low scientific production in regions such as India, Southeast Asia, Latin America; the overrepresentation of the health sector in the specialized literature. These findings seek to motivate researchers to fill gaps in scientific production and help managers and administrators to strengthen practices that improve the environmental conditions of workers. The limitations of this study are related to the characteristics of quantitative bibliographic studies and the inclusion of only two constructs related to people’s well-being at work, which suggests that future research could incorporate other variables such as emotional intelligence, leadership or organizational citizenship behavior. Full article
(This article belongs to the Section Organizational Behavior)
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19 pages, 2391 KB  
Article
Public Mining Governance for Sustainable Artisanal Gold Mining: Preventing Mercury Pollution in South America
by Jacopo Seccatore, Tatiane Marin, Jorge Tarra-Almario and Oscar J. Restrepo-Baena
Sustainability 2025, 17(19), 8894; https://doi.org/10.3390/su17198894 - 7 Oct 2025
Abstract
Artisanal and small-scale gold mining (ASGM) constitutes an essential livelihood strategy for marginalized communities, yet it is also associated with severe environmental and social challenges. Persistent inequality and poverty underpin miners’ dependence on informal practices, where access to safer technologies is limited. Mercury [...] Read more.
Artisanal and small-scale gold mining (ASGM) constitutes an essential livelihood strategy for marginalized communities, yet it is also associated with severe environmental and social challenges. Persistent inequality and poverty underpin miners’ dependence on informal practices, where access to safer technologies is limited. Mercury use remains critical in ASGM, often mismanaged in processing, applied in amalgamation, and released into air, water, and soils. An estimated 1000–2000 tonnes are emitted annually despite Minamata Convention commitments. This paper examines how mining governance can foster sustainable transitions in ASGM, focusing on the Chilean National Mining Company (ENAMI) as a case study. ENAMI’s model—combining regulatory oversight, institutional support, and inclusive decision-making—shows how public governance can reduce mercury reliance, mitigate environmental degradation, and enhance social inclusion. Findings highlight the importance of regulation, community participation, capacity-building, and addressing structural inequalities as integral components of sustainability. The study argues that sustainable ASGM requires not only technical innovation but also culturally sensitive institutional reforms capable of overcoming resistance and promoting long-term behavioral change. By situating ASGM within the broader framework of sustainable rural development, this research provides insights for policymakers, practitioners, and scholars seeking to reconcile economic inclusion with environmental stewardship in vulnerable contexts. Full article
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32 pages, 990 KB  
Article
Explaining the Determinants of International Financial Reporting Standard (IFRS) Disclosure: Evidence from Latin American Countries
by Rosa Isabel González Muñoz, Yeny Esperanza Rodríguez and Stella Maldonado
J. Risk Financial Manag. 2025, 18(10), 567; https://doi.org/10.3390/jrfm18100567 - 7 Oct 2025
Abstract
This study investigates the firm- and country-level determinants that influence the extent of financial disclosure under International Financial Reporting Standards (IFRS) in selected Latin American Organisation for Economic Co-operation and Development (OECD) members or countries in the accession process in the period under [...] Read more.
This study investigates the firm- and country-level determinants that influence the extent of financial disclosure under International Financial Reporting Standards (IFRS) in selected Latin American Organisation for Economic Co-operation and Development (OECD) members or countries in the accession process in the period under analysis. Using a sample of 168 publicly listed companies from Argentina, Chile, Colombia, Mexico, and Peru, we construct a self-developed disclosure index based on compliance with International Accounting Standards IAS 16 (Property, Plant and Equipment) and IAS 2 (Inventories). These standards were selected due to their relevance across a broad range of sectors in emerging markets. Drawing on agency theory, stakeholder theory, institutional theory, signaling theory, and legitimacy theory, we examine how internal firm characteristics, macroeconomic performance, and institutional quality impact disclosure practices. Our empirical findings show that firm size, leverage, Gross Domestic Product (GDP) growth, and shareholder protection have a positive and statistically significant influence on the level of IFRS disclosure. However, not all institutional variables are equally effective, highlighting the complex interplay between regulatory environments and corporate reporting behavior in developing countries. The study contributes to the ongoing debate on the applicability and effectiveness of IFRS in emerging economies by offering evidence from underexplored Latin American markets and emphasizing the need for context-specific policy and regulatory interventions. Full article
(This article belongs to the Special Issue Financial Reporting and Auditing)
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17 pages, 1470 KB  
Article
Stem-Centered Drought Tolerance in Mikania micrantha During the Dry Season
by Minling Cai, Minghao Chen, Junjie Zhang and Changlian Peng
Int. J. Mol. Sci. 2025, 26(19), 9722; https://doi.org/10.3390/ijms26199722 - 6 Oct 2025
Viewed by 24
Abstract
Mikania micrantha, commonly known as mile-a-minute weed, is listed among the world’s top 10 worst weeds. Although native to humid regions of South America, it has recently been found to colonize arid habitats as well. Despite pronounced seasonal hydroclimatic variations in South [...] Read more.
Mikania micrantha, commonly known as mile-a-minute weed, is listed among the world’s top 10 worst weeds. Although native to humid regions of South America, it has recently been found to colonize arid habitats as well. Despite pronounced seasonal hydroclimatic variations in South China and increasing drought due to global climate change, the mechanisms underlying M. micrantha’s drought tolerance remain poorly understood. In this study, we compared the photosynthetic responses of M. micrantha leaves and stems between the dry (June) and wet (December) seasons through field experiments. We measured changes in phenotype, photosynthetic characteristics, and the content of antioxidant and osmotic adjustment substances, using the co-occurring native vine Paederia scandens as a control. The results revealed that during the dry season, M. micrantha leaves exhibited wilting, along with significant reductions in relative water content (RWC), chlorophyll (Chl), soluble sugar (SS), and soluble protein (SP). In contrast, the stems of M. micrantha maintained relatively stable phenotypes and chlorophyll levels compared to those of P. scandens. Notably, M. micrantha stems exhibited significant increases in vessel wall thickness, vessel density, total phenol content, and the activities of peroxidase (POD) and ascorbate peroxidase (APX). Furthermore, compared to P. scandens, M. micrantha stems displayed a greater increase in cortex proportion, flavonoid content, and soluble protein content. Expression analysis of bZIP transcription factors further revealed drought-responsive upregulation of specific genes (bZIP60, ZIP42-1), suggesting their potential involvement in drought response. These results indicate that although the leaves of M. micrantha are susceptible to prolonged drought, the stems exhibit considerable resilience, which may be attributed to a combination of traits including structural modifications in stem anatomy, enhanced antioxidant capacity, and osmotic adjustment. These insights suggest that stem-specific adaptations are key to its drought tolerance, providing a theoretical foundation for understanding the habitat distribution of M. micrantha and informing effective management strategies. Full article
(This article belongs to the Section Molecular Plant Sciences)
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17 pages, 5330 KB  
Article
Global Potential Distribution of Carpomya vesuviana Costa Under Climate Change and Potential Economic Impacts on Chinese Jujube Industries
by Jingxuan Ning, Ming Li, Yuhan Qi, Haoxiang Zhao, Xiaoqing Xian, Jianyang Guo, Nianwan Yang, Hongxu Zhou and Wanxue Liu
Agriculture 2025, 15(19), 2081; https://doi.org/10.3390/agriculture15192081 - 6 Oct 2025
Viewed by 61
Abstract
Carpomya vesuviana (Diptera: Tephritidae), a significant invasive forestry pest of Zizyphus crops worldwide, has spread globally across jujube-growing regions, causing substantial yield losses and economic damage. In China, it is classified as both an imported and forestry quarantine pest. Existing risk assessments have [...] Read more.
Carpomya vesuviana (Diptera: Tephritidae), a significant invasive forestry pest of Zizyphus crops worldwide, has spread globally across jujube-growing regions, causing substantial yield losses and economic damage. In China, it is classified as both an imported and forestry quarantine pest. Existing risk assessments have primarily focused on the potential geographical distributions (PGDs) of C. vesuviana, but its economic impact on host plants is unknown. Therefore, we used an optimised MaxEnt model based on species distribution records and relevant environmental variables to predict the PGDs of C. vesuviana under current and future climate scenarios. Meanwhile, we used the @RISK stochastic model to assess the economic impact of this pest on the Chinese jujube industry under various scenarios. The results showed that the human influence index (HII), mean temperature of the wettest quarter (Bio8), temperature seasonality (Bio4), and precipitation during the driest month (Bio14) were the significant environmental variables affecting species distribution. Under the current climatic scenario, the total suitable area of C. vesuviana reached 2171.39 × 104 km2, which is mainly distributed in southern and western Asia, southern Europe, central North America, western Africa, and eastern South America. Potentially suitable habitats will increase and shift to the middle and high latitudes of the Northern Hemisphere under future climatic scenarios. Under the no-control scenario, C. vesuviana could cause losses of 15,687 million CNY to the jujube industry in China. However, control measures could have saved losses of 5047 million CNY. This study provides a theoretical basis for preventive monitoring and integrated management of C. vesuviana globally and helps reduce its economic impact on the jujube industry in China. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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17 pages, 1534 KB  
Article
Improving Telenomus remus (Hymenoptera: Scelionidae) Adoption: Contribution of Different Egg Parasitoid Densities, Fed Adults, and Their Storage for Successful Biological Control of Spodoptera frugiperda (Lepidoptera: Noctuidae)
by Weidson P. Sutil, Adeney de F. Bueno, Leonardo Roswadoski, Rafael S. Iasczczaki, Gabriel S. Carneiro and Yelitza C. Colmenarez
Insects 2025, 16(10), 1032; https://doi.org/10.3390/insects16101032 - 6 Oct 2025
Viewed by 171
Abstract
Egg parasitoids, such as Telenomus remus (Hymenoptera: Scelionidae), face significant challenges after release, as their pupae are exposed to various mortality factors that reduce the efficiency of biological control programs. Therefore, this study aimed to evaluate a honey-solid diet that can feed adults [...] Read more.
Egg parasitoids, such as Telenomus remus (Hymenoptera: Scelionidae), face significant challenges after release, as their pupae are exposed to various mortality factors that reduce the efficiency of biological control programs. Therefore, this study aimed to evaluate a honey-solid diet that can feed adults still inside the capsules without sticking the wasps on its surface, enabling parasitoid storage and later field release. Three independent bioassays were performed, each with 20 completely randomized replications. The first bioassay evaluated the acceptance of a solid feed—honey soaked in cotton thread—compared to the traditional form—honey droplets. In the second bioassay, the storage periods after emergence of adults in capsules with honey-solid food were analyzed at 2, 4, 6, and 8 days post-emergence, and the third bioassay studied the efficacy of different release densities of fed adults under field conditions. Parasitoids fed on the honey-solid diet exhibited a 13.3% reduction in parasitism compared to honey droplets. However, the sticky, viscous nature of honey can lead to parasitoids becoming glued, potentially leading to their death. T. remus feeding on the honey-solid diet resulted in low mortality inside the capsules, living up to six days with only 22.2% reduction in parasitism capacity, making it a viable alternative to release and transport fed adult parasitoids, with an increase of around 30% in the released density of parasitoids compared with the parasitoids fed on honey droplets. This flexibility of releasing T. remus up to six days after emergence provided valuable knowledge to establish T. remus as a biocontrol agent. Furthermore, the highest tested parasitoid density of 20,000 parasitoids per hectare obtained the highest parasitism of Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. However, future studies are still required with higher releasing densities and less expensive methods of mass rearing the parasitoid for those higher densities to be economically viable. Full article
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17 pages, 8322 KB  
Article
Housing Affordability in the United States: Price-to-Income Ratio by Pareto Distribution
by Francisco Vergara-Perucich
Geographies 2025, 5(4), 57; https://doi.org/10.3390/geographies5040057 - 6 Oct 2025
Viewed by 66
Abstract
This study integrates the price-to-income ratio (PIR) with Pareto distribution characteristics to provide a novel approach for evaluating home affordability across U.S. counties. The methodology offers a new lens for the analysis of home affordability by capturing both the extreme values and central [...] Read more.
This study integrates the price-to-income ratio (PIR) with Pareto distribution characteristics to provide a novel approach for evaluating home affordability across U.S. counties. The methodology offers a new lens for the analysis of home affordability by capturing both the extreme values and central tendencies of PIR. The study normalizes the resulting Pareto parameters to a common scale and integrates data from the Zillow Home Value Index and the U.S. Department of Commerce’s SAIPE program to create a single affordability index. The findings point to significant regional differences: coastal and urban regions, such as California and New York, face significant affordability challenges, whereas the Midwest, especially Kansas, has higher affordability. The results highlight the significance of targeted policy interventions and are consistent with the body of research on systemic risk and housing market dynamics. This study also opens new avenues for future research, including the impact of economic factors on affordability and cross-regional comparative studies. The suggested approach encourages more equitable access to housing by providing policymakers with a useful tool to track and manage challenges related to housing affordability. Full article
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25 pages, 3842 KB  
Review
Cranberry Research Progress: A Systematic Review of Chemical Composition, Pharmacological Mechanisms, Clinical Applications, and Nutritional Significance
by Jiao Xu, Chenliang Li, Wei Wu, Lingyang Kong, Lijin Xiao, Wei Ma and Lihong Zhang
Int. J. Mol. Sci. 2025, 26(19), 9707; https://doi.org/10.3390/ijms26199707 - 6 Oct 2025
Viewed by 170
Abstract
Cranberry (Vaccinium macrocarpon Ait.) is an herbaceous, evergreen, dwarf shrub of the genus Vaccinium in the family Ericaceae, often used as a functional food. Cranberries are primarily distributed in the northern United States—including Massachusetts, Wisconsin, and Maine—as well as in Quebec, Canada; [...] Read more.
Cranberry (Vaccinium macrocarpon Ait.) is an herbaceous, evergreen, dwarf shrub of the genus Vaccinium in the family Ericaceae, often used as a functional food. Cranberries are primarily distributed in the northern United States—including Massachusetts, Wisconsin, and Maine—as well as in Quebec, Canada; the state of Columbia; Chile in South America; and northeastern Europe. They are also found in China’s Greater Khingan Range and Fuyuan City, Jiamusi, Heilongjiang Province. The plants thrive in cool environments and exhibit considerable adaptability to soil conditions, preferring acidic soils. Cranberries are rich in a variety of biologically active components, such as polyphenols (proanthocyanidins, chlorogenic acid, flavonols, anthocyanins, caffeic acid, etc.), triterpenoids, and other nutrients. Studies have shown that the chemical components extracted from cranberry fruit have pharmacological effects such as antioxidant, anti-inflammatory, anti-cancer, and urinary tract infection prevention and treatment, and are commonly used clinically in the treatment of cardiovascular diseases, the prevention of urinary tract infections, blood pressure lowering, and the fight against Helicobacter pylori, among other clinical diseases. Cranberries also play a huge role in daily nutrition, and they are named for their richness in a variety of mineral elements, trace elements and vitamins. This work uses information from Pubmed, Web of Science, Scopus, CNKI (China National Knowledge Infrastructure), and related papers. In this paper, a comprehensive review of the phytochemical composition, pharmacological mechanism of action, clinical application value and nutritional significance of cranberry was conducted in recent years to provide references for the further extraction of chemical components in cranberry and rational clinical application, which can help to guide people to rationalize their diets and promote the formation of healthy diets. Full article
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12 pages, 258 KB  
Article
Enhancing Research Visibility: A Comparative Study on the Implementation of CRIS Systems at Universidad Católica de Santa María and Its Contrast with Other Universities
by Javier Fernando Angulo-Osorio, César Daniel Valdivia-Portugal and Karina Rosas-Paredes
Publications 2025, 13(4), 51; https://doi.org/10.3390/publications13040051 - 5 Oct 2025
Viewed by 175
Abstract
Research visibility has become a critical issue for universities, yet the institutional conditions that shape it remain underexplored. While Current Research Information Systems (CRISs) provide essential infrastructure for managing publications and researcher profiles, their impact depends on broader governance and cultural factors. This [...] Read more.
Research visibility has become a critical issue for universities, yet the institutional conditions that shape it remain underexplored. While Current Research Information Systems (CRISs) provide essential infrastructure for managing publications and researcher profiles, their impact depends on broader governance and cultural factors. This study compares four universities—two in Peru, one in Chile, and one in Spain—that have adopted the Pure CRIS platform. Data were manually extracted from institutional portals and analyzed descriptively, using normalized indicators such as publications per researcher, Sustainable Development Goal (SDG) alignment, and collaboration networks. Although based on a limited sample, the analysis highlights substantial contrasts: European institutions show consolidated integration of CRIS into national evaluation systems, while Latin American universities remain at earlier stages of adoption, with fragmented policies and limited international reach. The findings suggest that technological platforms alone are insufficient; institutional commitment, coherent policies, and academic cultures that value dissemination are decisive. These insights contribute a comparative framework to guide universities, particularly in Latin America, seeking to strengthen their global research visibility. Full article
13 pages, 276 KB  
Case Report
Spotted Fever Rickettsioses in Panama: New Cases and the Gaps That Hinder Its Epidemiological Understanding
by Sergio Bermúdez, Ericka Ferguson Amores, Naty Aguirre, Michelle Hernández, Boris Garrido, Lillian Domínguez, Yamitzel Zaldívar, Claudia González, Jorge Omar Castillo, Alexander Martínez-Caballero, Ambar Moreno, Mabel Martínez-Montero, Ambar Poveda, Domicio Espino, Karina Baker and Franklyn Samudio
Pathogens 2025, 14(10), 1006; https://doi.org/10.3390/pathogens14101006 - 4 Oct 2025
Viewed by 334
Abstract
Rickettsia rickettsii is the most virulent agent of the genus Rickettsia that causes one of the most relevant vector-borne diseases in the Americas (RRSF). RRSF manifests with many non-specific acute clinical symptoms complicating its diagnosis and can lead to death if not treated [...] Read more.
Rickettsia rickettsii is the most virulent agent of the genus Rickettsia that causes one of the most relevant vector-borne diseases in the Americas (RRSF). RRSF manifests with many non-specific acute clinical symptoms complicating its diagnosis and can lead to death if not treated appropriately. RRSF has been reported in Canada, the United States of America, Mexico, Costa Rica, Panama, Colombia, Brazil, and Argentina. In addition to R. rickettsii, mild and severe spotted fever group rickettsioses (SFGR) have been reported in the Americas; however, the true prevalence of these diseases is unknown. In Panama, RRSF have been reported in four of 14 provinces during two outbreak periods: five cases including two fatalities were identified in 1950–1951, and 23 cases including 17 fatalities between 2004 and 2025. This paper presents the clinical characterization of a fatal case of RRSF in Coclé province and a severe case of SFGR in a mountainous area of the Gnäbe Buglé Indigenous Comarca (GBIC). Laboratory confirmation was performed by molecular analysis of tissues obtained from necropsies in the case of RRSF and by immunofluorescence assay (IFA) in the case of SFGR. Furthermore, this paper identifies existing gaps in the initial clinical suspicion and pertinent to SFGR in Panama, which may be applicable to other countries in the region. In the last 21 years, cases have occurred upon contact with ticks in rural areas (13), urban and suburban locations (7), rural woodlands (2), and forests (1). Provinces with more cases are Panamá (7 of 23, 6 died), Coclé (5 of 23, 5 died), Colón (3 of 23, 1 died), Panamá Oeste (1 of 23, 1 died), and GBIC (7 of 23, 4 died), including a cluster of seven cases in 2019. Therefore, Coclé province is considered one of the endemic areas for RRSF in Panama, while the latest cases from the GBIC since 2019 indicate that mountainous areas are an eco-epidemiological scenario to include in the transmission of these diseases. Although this disease has a low prevalence, patients who present symptoms commonly associated with more common diseases such as dengue, other arboviruses, malaria, and leptospirosis, among others, should be included in the diagnostic suspicion. Without diagnostic suspicion and adequate treatment, the patient can die. Full article
(This article belongs to the Collection Advances in Tick Research)
19 pages, 1585 KB  
Article
How Science Supports Honey Bees: Identification of Research on Best Practices in Beekeeping
by Kristina Gratzer, Veronika Musalkova and Robert Brodschneider
Insects 2025, 16(10), 1025; https://doi.org/10.3390/insects16101025 - 4 Oct 2025
Viewed by 357
Abstract
Honey bee health and productivity are strongly linked to management practices and biosecurity measures. We collected and analyzed 744 practice records from 191 peer-reviewed field studies published since 1995, each documenting the impact of a specific hive intervention on colony health or productivity [...] Read more.
Honey bee health and productivity are strongly linked to management practices and biosecurity measures. We collected and analyzed 744 practice records from 191 peer-reviewed field studies published since 1995, each documenting the impact of a specific hive intervention on colony health or productivity parameters. Practices were categorized into good beekeeping practices (n = 128, 17.2%) and biosecurity measures (n = 616, 82.8%) and grouped by management or pathogen theme, geographic region, and season. Most research originated from Europe (34.6%) and North America (33.4%), with nearly all focused on Apis mellifera (99.9%). Varroa control dominated (57.0%), followed by general apiary management (17.2%) and american foulbrood (9.7%). For varroosis, “soft” acaricides such as oxalic and formic acid accounted for 58.5% of records, while “hard” synthetic products represented 21.0%. Within general apiary management, colony management was most frequent (46.9%). For american foulbrood, antibiotics (41.7%) and biotechnical methods (22.2%) were prominent. Tropilaelaps app. treatments relied mainly on “soft” acaricides (81.5%), while small hive beetle control focused on in-hive traps (55.9%). Seasonally, most interventions occurred from August to October, with AFB measures peaking in spring and early summer. The dataset highlights regional research gaps and offers a structured, expandable framework to guide future research and support evidence-based decision-making in beekeeping and advisory services. Full article
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14 pages, 692 KB  
Article
Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
by Guillermo Giovambattista, Aronggaowa Bao, Olivia Marcuzzi, Ariel Loza Vega, Juan Antonio Pereira Rico, Maria Florencia Ortega Masague, Liz Aurora Castro Rojas, Ruben Dario Martinez, Odalys Uffo Reinosa and Yoko Aida
Pathogens 2025, 14(10), 1005; https://doi.org/10.3390/pathogens14101005 - 4 Oct 2025
Viewed by 199
Abstract
Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin [...] Read more.
Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance. Full article
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Article
Subterranean Biodiversity on the Brink: Urgent Framework for Conserving the Densest Cave Region in South America
by Robson de Almeida Zampaulo, Marconi Souza-Silva and Rodrigo Lopes Ferreira
Animals 2025, 15(19), 2899; https://doi.org/10.3390/ani15192899 - 3 Oct 2025
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Abstract
Subterranean ecosystems represent some of the most unique and fragile habitats on Earth, yet they remain poorly understood and highly vulnerable to human-induced disturbances. Despite their ecological significance, these systems are rarely integrated into conservation planning, and surface-level protected areas alone are insufficient [...] Read more.
Subterranean ecosystems represent some of the most unique and fragile habitats on Earth, yet they remain poorly understood and highly vulnerable to human-induced disturbances. Despite their ecological significance, these systems are rarely integrated into conservation planning, and surface-level protected areas alone are insufficient to safeguard their biodiversity. In southeastern Brazil, a karst landscape spanning approximately 1200 km2, recognized as the region with the highest cave density in South America (approximately 2600 caves), is under increasing pressure from urban expansion, agriculture, and mining, all of which threaten the ecological integrity of subterranean habitats. This study sought to identify caves of high conservation priority by integrating species richness of non-troglobitic invertebrates, occurrence of troglobitic species, presence of endemic troglobitic taxa, and the degree of anthropogenic impacts, using spatial algebra and polygon-based mapping approaches. Agriculture and exotic forestry plantations (54%) and mining operations (15%) were identified as the most prevalent disturbances. A total of 32 troglobitic species were recorded, occurring in 63% of the 105 surveyed caves. Notably, seven caves alone harbor 25% of the region’s known cave invertebrate diversity and encompass 50% of its cave-restricted species. The findings highlight the global significance of this spot of subterranean biodiversity and reinforce the urgent need for targeted conservation measures. Without immediate action to mitigate unsustainable land use and resource exploitation, the persistence of these highly specialized communities is at imminent risk. Full article
(This article belongs to the Section Ecology and Conservation)
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