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24 pages, 3933 KB  
Article
Evaluation of the Construction Suitability and Sediment Reduction Potential of Dry-Farming Wide Terraces on Sloping Farmland in the Loess Plateau
by Ying Han, Wenjing Wang, Xinjia Chen, Jinxia Fu, Ruizhe Du and Bo Li
Land 2026, 15(5), 747; https://doi.org/10.3390/land15050747 - 28 Apr 2026
Viewed by 58
Abstract
Assessing the construction suitability and sediment reduction potential of dry-farming wide terraces is critical for improving soil and water conservation in semi-arid and semi-humid regions, yet these aspects are seldom evaluated within an integrated framework. Focusing on the Loess Plateau, this study delineates [...] Read more.
Assessing the construction suitability and sediment reduction potential of dry-farming wide terraces is critical for improving soil and water conservation in semi-arid and semi-humid regions, yet these aspects are seldom evaluated within an integrated framework. Focusing on the Loess Plateau, this study delineates potential construction areas based on precipitation constraints, quantifies soil erosion using the Revised Universal Soil Loss Equation, and develops a multidimensional framework to jointly evaluate construction suitability and sediment reduction potential on sloping farmland. Results indicate that slope, transportation accessibility, and soil erosion intensity are the primary determinants of suitability. Highly suitable, suitable, and marginally suitable areas account for 7.5%, 7.2%, and 4.3% of the study area, respectively, with Shanxi, Shaanxi, and Gansu provinces—and particularly Yulin, Yan’an, and Qingyang—emerging as priority regions for implementation. Scenario analysis suggests that targeting (i) highly suitable and suitable areas or (ii) all suitable classes would reclaim approximately 59.89 × 103 km2 and 77.19 × 103 km2 of sloping farmland, respectively, leading to reductions in mean soil erosion modulus of 16.6% and 22%. These findings provide a quantitative basis for optimizing terrace deployment and advancing regionally targeted soil erosion mitigation strategies on the Loess Plateau. Full article
(This article belongs to the Special Issue Feature Papers on Land Use, Impact Assessment and Sustainability)
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15 pages, 19143 KB  
Article
Revealing the Dynamic Association Between Lymphatic Endothelial Cell Markers and Intervertebral Disk Degeneration
by Qiang Zhang, Maoqiang Lin, Shishun Yan, Fei Huang and Haiyu Zhou
Biomedicines 2026, 14(5), 993; https://doi.org/10.3390/biomedicines14050993 - 27 Apr 2026
Viewed by 313
Abstract
Objective: This study aims to analyze the dynamic changes in lymphatic endothelial cell (LEC) markers during the progression of intervertebral disk degeneration (IDD) and to investigate their association with the progression of IDD. Method: In this study, intervertebral disk (IVD) specimens were first [...] Read more.
Objective: This study aims to analyze the dynamic changes in lymphatic endothelial cell (LEC) markers during the progression of intervertebral disk degeneration (IDD) and to investigate their association with the progression of IDD. Method: In this study, intervertebral disk (IVD) specimens were first collected from patients who underwent open lumbar fusion surgery for spinal fractures (control group, n = 10) and lumbar disk herniation (IDD group, n = 10). Concurrently, a mouse IDD model was established, and IVD specimens were collected from mouse in the Sham group and the IDD group 1, 3, and 6 weeks after modeling (n = 5 per group at each time point). Pathological morphological changes in human and mouse IVD specimens were observed using Hematoxylin and Eosin (H&E) and Masson’s Trichrome staining. The degree of degeneration in the mouse IVD specimens was quantified using a histopathological scoring system. Subsequently, real-time quantitative polymerase chain reaction (RT-qPCR), immunohistochemistry (IHC), and immunofluorescence (IF) staining were employed to examine LEC markers in IVD tissue, including lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), podoplanin (PDPN), prospero homeobox protein 1 (PROX-1), and vascular endothelial growth factor receptor 3 (VEGFR-3), as well as matrix metabolism-related markers such as matrix metalloproteinase 13 (MMP-13) and collagen II (Col II). Finally, we performed Spearman’s rank correlation analysis between the histopathological scores of all mouse IVD specimens and the corresponding expression levels of LEC markers. Results: In human IVD tissue, expression levels of LYVE-1, PDPN, PROX-1, and VEGFR-3 were extremely low in the normal group. In contrast, expression of these markers was significantly upregulated in the IDD group. In the mouse IDD model, compared with the Sham group at the same time point, the IDD group exhibited higher histopathological scores in IVD tissue, accompanied by upregulation of LYVE-1, PDPN, PROX-1, and MMP-13, as well as downregulation of Col II. In-depth analysis revealed that these differences between the Sham and IDD groups were not static but exhibited a dynamic pattern of increasing magnitude over time. Concurrently, as the modeling period progressed, the histopathological scores of mouse IVD in the IDD group, as well as the expression levels of LYVE-1, PDPN, PROX-1, and MMP-13, showed a progressive upward trend, while Col II expression progressively decreased. In addition, Spearman’s rank correlation analysis revealed that the expression levels of LYVE-1, PDPN, and PROX-1 in mouse IVD tissue were all significantly positively correlated with histopathological scores. Conclusions: In the process of IDD, the dynamic upregulation of LEC markers is highly consistent with its severity in the time dimension. At the same time, there was also a significant positive correlation between the expression level of LEC markers and the severity of IDD. Taken together, these findings suggest that the dynamic upregulation of LEC markers may be potentially associated with the pathological progression of IDD. Full article
(This article belongs to the Section Molecular and Translational Medicine)
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18 pages, 5295 KB  
Article
Overexpression of the Large-Conductance Mechanosensitive Channel Gene in Oenococcus oeni Enhances Its Ethanol Stress Tolerance
by Longxiang Liu, Yang Zhao, Lemeng Zhang, Yujuan Zheng, Shuai Peng, Hongyu Zhao, Xinyu Zhao, Yumiao Zhang, Jingjing Fang and Weiyu Song
Microorganisms 2026, 14(5), 973; https://doi.org/10.3390/microorganisms14050973 - 26 Apr 2026
Viewed by 149
Abstract
Oenococcus oeni (O. oeni) can initiate and complete the malolactic fermentation (MLF) process, which significantly improves wine quality. However, stress factors commonly encountered in wine, such as acid stress and ethanol stress, can hinder this process. Overexpression of certain key functional [...] Read more.
Oenococcus oeni (O. oeni) can initiate and complete the malolactic fermentation (MLF) process, which significantly improves wine quality. However, stress factors commonly encountered in wine, such as acid stress and ethanol stress, can hinder this process. Overexpression of certain key functional genes using genetic recombination technology can enhance the stress tolerance of O. oeni. In this study, the large-conductance mechanosensitive channel (mscl) gene was overexpressed in O. oeni SD-2a using genetic recombination technology. The results showed that overexpression of this gene enhanced the growth rate of O. oeni under 10% ethanol stress conditions. Physiological index measurements indicated that overexpression of this gene enhanced the control of cell membrane permeability in the recombinant strain at different time points under ethanol stress and altered cell membrane fluidity at these time points. Proteomic analysis after 12 h of treatment under 10% ethanol stress revealed that mscl overexpression significantly altered the protein expression pattern of O. oeni. The most significantly affected proteins included some cell membrane transporters (for sugars, lipids, amino acids, and nucleotides) and proteins involved in cell wall synthesis. These results suggest that mscl overexpression enhances the ethanol stress tolerance of O. oeni by altering its cell membrane properties and affecting the expression levels of proteins related to cell membrane transport and cell wall synthesis. This study provides a theoretical reference for obtaining O. oeni recombinant strains with enhanced stress tolerance through genetic recombination technology. Full article
(This article belongs to the Section Food Microbiology)
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15 pages, 3268 KB  
Article
Assessing Climate-Driven Range Dynamics of Hippophae tibetana Schltdl. Using an Ensemble Modeling Approach
by Tao Ma, Biyu Liu, Danping Xu and Zhihang Zhuo
Diversity 2026, 18(5), 257; https://doi.org/10.3390/d18050257 - 26 Apr 2026
Viewed by 174
Abstract
Hippophae tibetana Schltdl. is a cold-tolerant deciduous shrub endemic to the Tibetan Plateau, playing a vital ecological role in high-altitude environments. This study utilized the Biomod2 platform to model its current and future potential distribution under climate change, integrating 34 environmental variables across [...] Read more.
Hippophae tibetana Schltdl. is a cold-tolerant deciduous shrub endemic to the Tibetan Plateau, playing a vital ecological role in high-altitude environments. This study utilized the Biomod2 platform to model its current and future potential distribution under climate change, integrating 34 environmental variables across bioclimatic, topographic, edaphic, anthropogenic, and ultraviolet (UV) dimensions. Among ten candidate species distribution models (SDMs), the random forest (RF) algorithm exhibited the highest predictive accuracy and stability. An ensemble model (EM) combining RF, GBM, MARS, and FDA further improved predictive performance (ROC = 0.992, TSS = 0.923, and Kappa = 0.886). Key determinants of habitat suitability included altitude, temperature, UV radiation, and biodiversity, with RF response curves revealing distinct nonlinear thresholds. Optimal suitability occurred at around a 4000 m elevation, decreasing beyond this range, while temperature and UV exhibited similar unimodal responses. Under the SSP2-4.5 climate scenario, the suitable habitat is projected to expand from the 2050s to the 2090s, particularly in eastern Qinghai, southwestern Gansu, northwestern Sichuan, and central–southern Tibet. The species’ distribution centroid is anticipated to shift southwestward toward Qinghai Province, with more rapid migration projected after the 2050s. These findings underscore the complex interplay of environmental factors shaping H. tibetana distribution and offer valuable insights for conservation planning in the ecologically fragile Tibetan Plateau. Full article
(This article belongs to the Section Biodiversity Conservation)
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20 pages, 5741 KB  
Article
Effects of Reduced Irrigation on Growth, Yield and Water Use Efficiency of Potato Under Drip Irrigation with Plastic Mulch
by Pengde Chen, Jinyong Zhu, Zhitao Li, Xiaoqiang Qiu, Minmin Bao, Panfeng Yao, Zhenzhen Bi, Yuanming Li, Yuhui Liu and Zhen Liu
Agronomy 2026, 16(9), 866; https://doi.org/10.3390/agronomy16090866 - 24 Apr 2026
Viewed by 275
Abstract
Water scarcity is the primary constraint on the development of the potato industry in Northwest China. Improving water use efficiency (WUE) under limited water supply is, therefore, an urgent priority to promote the green and sustainable development of potato production in this region. [...] Read more.
Water scarcity is the primary constraint on the development of the potato industry in Northwest China. Improving water use efficiency (WUE) under limited water supply is, therefore, an urgent priority to promote the green and sustainable development of potato production in this region. This research was conducted from 2023 to 2024 in the rain shelter of the Agricultural Science Research Institute in Dingxi City, Gansu Province, using the potato cultivar ‘Gan Yin No. 9’ as the experimental material. Throughout the growing season, the control treatment (CK) was maintained at 75–85% of the field water capacity (FWC). Based on CK, three deficit-irrigation treatments were established: W75 (75% of the CK irrigation amount), W50 (50% of CK irrigation amount), and W25 (25% of CK irrigation amount), with three replicates per treatment. We evaluated the effects of different irrigation regimes on plant growth characteristics, physiological characteristics, tuber yield, and WUE. The results showed that the W75 treatment significantly (p < 0.05) promoted the growth of plant height and stem diameter, and significantly increased them by 8.70–10.20% and 13.03–18.70%, respectively, compared with CK. The total dry matter accumulation under W75 was significantly higher than CK (by 10.90–11.40%) and markedly higher than W50 and W25 (by 24.10–45.50%). No significant differences were observed in tuber yield, large tuber rate, and medium tuber rate between W75 and CK. Notably, W75 significantly improved WUE by 36.43–38.51% compared with CK. Overall, under the conditions of this study, W75 treatment was identified to be the optimal irrigation regime for potato cultivation, as it promoted plant growth, maintained tuber yield, and enhanced water use efficiency. This study aims to establish a definitive irrigation threshold for potato production in Northwest China. The findings provide a precise basis for formulating irrigation schedules, which can contribute to the development of water-efficient agriculture and support the sustainable development of the potato industry in the region. Full article
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27 pages, 9070 KB  
Article
Optimized Straw Strip Mulching Enhances Soil Water–Heat–Carbon Synergy and Stabilizes Winter Wheat Yield in Semi-Arid Regions
by Chenxin Huang, Junsheng Lu, Yuwei Chai, Meng Zhou, Baozhan Li, Lei Chang, Rui Jia and Caixia Huang
Agronomy 2026, 16(9), 859; https://doi.org/10.3390/agronomy16090859 - 24 Apr 2026
Viewed by 242
Abstract
To address water-heat constraints and environmental risks associated with plastic film mulching in winter wheat production in the semi-arid region of Northwest China, a two-year field experiment (2021–2023) was conducted in Tongwei County, Gansu Province. A single-factor randomized block design was applied, with [...] Read more.
To address water-heat constraints and environmental risks associated with plastic film mulching in winter wheat production in the semi-arid region of Northwest China, a two-year field experiment (2021–2023) was conducted in Tongwei County, Gansu Province. A single-factor randomized block design was applied, with full plastic film mulching (PM) and bare land (CK) as controls, to evaluate the effects of 3-row (S3), 4-row (S4), and 5-row (S5) corn stalk strip mulching on soil hydrothermal conditions, active carbon fractions, and yield under rainfed conditions. Results showed that straw mulching significantly enhanced soil water retention, particularly in the 0–40 cm layer, where moisture content increased by 7.70–19.28% compared with CK (p < 0.05), with S3 performing best. Treatment S5 achieved the highest accumulated temperature and reduced the soil diurnal temperature range by 20.73–35.62% (p < 0.05). Active carbon fractions were also significantly improved, especially during the jointing–grain-filling stage (BBCH 31–87). In terms of yield, S5 exhibited the greatest increase, with a 15.88% higher two-year average grain yield than CK (p < 0.05), reaching over 90% of PM. Overall, S5 demonstrated optimal synergistic regulation of water, heat, and carbon, indicating strong potential as a sustainable alternative to plastic film mulching. Full article
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53 pages, 2489 KB  
Review
An Updated Checklist of the Phytophagous Ladybird Beetles (Coccinellinae: Epilachnini) of China
by Muhammad Asghar Hassan, Bing-Lan Zhang, Zafar Iqbal, Muhammad Ali, Yi-Fei Sun, Taslima Sheikh, Hao-Sen Li and Hong Pang
Insects 2026, 17(5), 450; https://doi.org/10.3390/insects17050450 (registering DOI) - 24 Apr 2026
Viewed by 382
Abstract
A comprehensive annotated checklist of the members of the phytophagous ladybird beetle tribe Epilachnini (Coccinellinae) in China is compiled based on existing published sources and incorporates the latest taxonomic and nomenclatural updates. The checklist documents 176 extant species across 10 genera and provides [...] Read more.
A comprehensive annotated checklist of the members of the phytophagous ladybird beetle tribe Epilachnini (Coccinellinae) in China is compiled based on existing published sources and incorporates the latest taxonomic and nomenclatural updates. The checklist documents 176 extant species across 10 genera and provides analyses of regional species richness, distribution, and host plant associations. Regarding regional species richness, Yunnan Province is home to the highest number of species (76), followed by Taiwan (50), Sichuan (48), Guizhou (48), Guangxi (43), Tibet (43), Guangdong (25), Hainan (17), Hubei (17), Hunan (13), Shaanxi (13), Fujian (12), Henan (10), Jiangsu (10), Anhui (7), Shandong (7), Zhejiang (7), Jiangxi (5), Hong Kong (5), Gansu (5), Beijing (4), Hebei (4), Liaoning (3), Shanxi (2), and Chongqing, Jilin, Heilongjiang, Ningxia, and Xinjiang (each with one species). Among the recognized genera, Epilachna Chevrolat, 1837, is currently the most species-rich genera, with 59 species, followed by Afissa Dieke, 1947 (34), Uniparodentata Wang & Cao, 1993 (28), Henosepilachna Li, 1961 (29), Afidentula Kapur, 1958 (10), Diekeana Tomaszewska & Szawaryn, 2015 (9), and Epiverta Dieke, 1947 (4). Additionally, Afidenta Dieke, 1947, Cynegetis Chevrolat, 1837, and Subcoccinella Agassiz & Erichson, 1845 are each represented by a single species. Host plant data are currently available for only 72 species (approximately 41% of the species recorded in China), which are associated with 177 plant species across 34 families. The most frequently recorded host plant families are Solanaceae (43 species), Cucurbitaceae (32), Urticaceae (15), Fabaceae (14), Asteraceae (14), and Poaceae (10), whereas each of the remaining 28 families comprises fewer than 10 host species. For 104 species (59% of the Chinese members of the tribe), host plant associations remain unknown, highlighting a substantial gap in our understanding of their feeding habits. Full article
(This article belongs to the Special Issue Insect Diversity: Coleoptera)
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21 pages, 24361 KB  
Article
Effects of Water-Retaining Agent Application on Growth Physiological Characteristics and Yield of Alfalfa (Medicago sativa L.)
by Minhua Yin, Mingzhu Wang, Wenqiong Ma, Yuanbo Jiang, Wenjing Chang, Yanxia Kang, Guangping Qi, Yanlin Ma and Guanheng Wu
Plants 2026, 15(9), 1304; https://doi.org/10.3390/plants15091304 - 23 Apr 2026
Viewed by 222
Abstract
In arid and semi-arid regions, the cultivation of artificial grasslands commonly suffers from low productivity due to insufficient water supply. The rational application of water-retaining agents is an important approach to alleviating production constraints in artificial grasslands facing resource-based water scarcity. This study [...] Read more.
In arid and semi-arid regions, the cultivation of artificial grasslands commonly suffers from low productivity due to insufficient water supply. The rational application of water-retaining agents is an important approach to alleviating production constraints in artificial grasslands facing resource-based water scarcity. This study investigated two types of water-retaining agents [starch-grafted acrylate water-retaining agent (B1) and polyacrylamide water-retaining agent (B2)] and four application rates [0 kg·hm−2 (CK), 30 kg·hm−2 (T1), 60 kg·hm−2 (T2), 90 kg·hm−2 (T3)], systematically analyzing their effects on the growth, osmotic adjustment substances, antioxidant enzyme activities, and yield of alfalfa. The results showed that alfalfa plant height, stem diameter, leaf area, branch number, soluble sugar (SS), soluble protein (SP), and proline (Pro) all exhibited a decreasing trend with increasing cutting times. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in alfalfa leaves initially increased and then decreased with increasing application rates of water-retaining agents, while malondialdehyde (MDA) content showed a decreasing trend. Under the B2T2 treatment, both alfalfa yield and water-use efficiency (WUE) reached their highest values, recorded as 4931.97 kg·hm−2 (2022), 6021.44 kg·hm−2 (2023) and 2.19 kg·m−3 (2022), 2.39 kg·m−3 (2023), respectively. Based on the principal component analysis for comprehensive evaluation, the B2T2 treatment (polyacrylamide water-retaining agent applied at 60 kg·hm−2) achieved the highest comprehensive score in both years and could synergistically improve alfalfa yield and water-use efficiency. However, its applicability in the Yellow River irrigation region of Gansu Province and similar ecological areas still requires further verification through field trials. Full article
(This article belongs to the Special Issue Water and Nutrient Management for Sustainable Crop Production)
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27 pages, 2093 KB  
Article
Flood Susceptibility Mapping and Runoff Modeling in the Upper Baishuijiang River Basin, China
by Hao Wang, Quanfu Niu, Jiaojiao Lei and Weiming Cheng
Remote Sens. 2026, 18(9), 1270; https://doi.org/10.3390/rs18091270 - 22 Apr 2026
Viewed by 153
Abstract
Mountain flood susceptibility in complex mountainous basins is strongly influenced by terrain–climate interactions; however, the linkage between spatial susceptibility patterns and hydrological processes remains poorly understood. This study proposes a process-oriented framework that explicitly links flood susceptibility patterns with hydrological processes, moving beyond [...] Read more.
Mountain flood susceptibility in complex mountainous basins is strongly influenced by terrain–climate interactions; however, the linkage between spatial susceptibility patterns and hydrological processes remains poorly understood. This study proposes a process-oriented framework that explicitly links flood susceptibility patterns with hydrological processes, moving beyond conventional approaches that rely on independent model integration. The Baishuijiang River Basin, located in Wenxian County, southern Gansu Province, China, is selected as a representative mountainous watershed for this analysis. The specific conclusions are as follows: (1) Flood susceptibility was mapped using a Particle Swarm Optimization (PSO)-enhanced Maximum Entropy (MaxEnt) model based on multi-source environmental variables, including climatic, terrain, soil, land cover, and vegetation factors. The model achieved high predictive accuracy (Area Under the Receiver Operating Characteristic Curve (AUC) = 0.912), identifying precipitation of the driest month (bio14), elevation, and land use as dominant controlling factors. Medium-to-high-susceptibility areas account for approximately 22% of the basin and are mainly distributed along river valleys and flow convergence areas. These patterns are strongly associated with reduced infiltration capacity under dry antecedent conditions and enhanced flow concentration in steep terrain, and they exhibit clear nonlinear responses and threshold effects. (2) Hydrological simulations using Hydrologic Engineering Center–Hydrologic Modeling System (HEC-HMS) show good agreement with observed runoff (Nash–Sutcliffe Efficiency (NSE) = 0.74−0.85). Sensitivity analysis indicates that runoff dynamics are primarily controlled by the Curve Number (CN), recession constant, and ratio to peak, corresponding to infiltration capacity, recession processes, and peak discharge amplification. The spatial consistency between high-susceptibility areas and areas of strong runoff response demonstrates that susceptibility patterns can be physically explained through hydrological processes, providing a process-based interpretation rather than a purely statistical prediction. (3) Future projections indicate that medium–high-susceptibility areas remain generally stable but show a gradual expansion (+5.2% ± 0.8%) and increasing concentration along river corridors under climate change scenarios. This reflects intensified precipitation variability and enhanced runoff concentration processes, suggesting a climate-driven amplification of flood risk in hydrologically connected areas. Overall, this study goes beyond conventional susceptibility assessment by establishing a physically interpretable framework that provides a consistent linkage between environmental controls, susceptibility patterns, and hydrological responses. The proposed approach is transferable to similar mountainous basins with strong terrain–climate interactions, although uncertainties related to data limitations and single-basin application remain and require further investigation. Full article
(This article belongs to the Special Issue Remote Sensing for Planetary Geomorphology and Mapping)
20 pages, 1501 KB  
Review
Review on the Mechanism of and Therapies Targeting PANoptosis in Ulcerative Colitis
by Mi Zhao, Min Liu, Wen Tian, Tiantian Ren, Jianing Jing, Ya Zheng and Zhaofeng Chen
Biomolecules 2026, 16(5), 624; https://doi.org/10.3390/biom16050624 - 22 Apr 2026
Viewed by 357
Abstract
Ulcerative colitis (UC) is a complex chronic inflammatory bowel disease, and its pathogenesis is closely related to immune imbalance, intestinal flora disorder and intestinal barrier damage. In recent years, a novel form of programmed cell death, PANoptosis, has been confirmed to play a [...] Read more.
Ulcerative colitis (UC) is a complex chronic inflammatory bowel disease, and its pathogenesis is closely related to immune imbalance, intestinal flora disorder and intestinal barrier damage. In recent years, a novel form of programmed cell death, PANoptosis, has been confirmed to play a core role in the pathological process of UC. PANoptosis is driven by the PANoptosome complex, which is assembled by key molecules such as ZBP1, NLRP3, and RIPK1, which can simultaneously activate pyroptosis, apoptosis, and necroptosis. This not only leads to damage to the intestinal epithelial barrier, but it also aggravates the dysfunction of immune cells by releasing a large amount of pro-inflammatory cytokines and damage-associated molecular patterns (DAMPs), thus forming a vicious cycle of “cell death and inflammation”. Given the complexity of the PANoptosis signaling network, the efficacy of single-target inhibitors is limited. This review systematically expounds the mechanism of action of PANoptosis in UC and focuses on discussing multi-target combination treatment strategies represented by smart hydrogels loaded with multiple inhibitors (such as MCC950, GSK772, VX-765, disulfiram, etc.). This strategy achieves synergy through “vertical blocking” and “horizontal coverage”, and in combination with targeted delivery to the lesion, provides a highly promising innovative direction for fundamentally breaking the pathological cycle of UC. Future research should focus on the development of new inhibitors, the optimization of delivery systems, and in-depth clinical translation to promote this strategy as a breakthrough therapy for refractory UC. Full article
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32 pages, 12423 KB  
Article
Pseudomonas fluorescens G3 Enhances the Salt Stress Tolerance of Maize and Modulates Soil Microbial Community Composition in the Rhizosphere
by Yue Lou, Chenying Wu, Xu Wang, Meiling Shi, Zhaoyu Li, Xu Su, Wenshuo Ye, Caiping Dai, Yongqiang Tian and Yang Liu
Plants 2026, 15(9), 1281; https://doi.org/10.3390/plants15091281 - 22 Apr 2026
Viewed by 275
Abstract
Soil salinization impacts over one billion hectares, threatening global food security. Here, a salt-tolerant bacterial strain, Pseudomonas fluorescens G3, was isolated from the rhizosphere of maize (Jinongyu-719) growing in saline–alkali soils in Gansu Province, China. This strain demonstrated the ability to secrete indole-3-acetic [...] Read more.
Soil salinization impacts over one billion hectares, threatening global food security. Here, a salt-tolerant bacterial strain, Pseudomonas fluorescens G3, was isolated from the rhizosphere of maize (Jinongyu-719) growing in saline–alkali soils in Gansu Province, China. This strain demonstrated the ability to secrete indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, and extracellular polysaccharides. It also exhibited notable phosphate-solubilizing activity and robust siderophore production capabilities. Under salt stress conditions (200 mM NaCl), the P. fluorescens G3 strain significantly improved maize’s growth parameters, namely its plant height, root length, and dry weight. Further, it enhanced antioxidant enzyme activity while reducing the accumulation of malondialdehyde (MDA), mitigating stress-induced oxidative damage. In P. fluorescens G3-inoculated plants, leaf and root Na+ contents decreased by 34.90% and 33.91%, while their K+ contents increased by 40.20% and 33.47%, respectively. Inoculation with P. fluorescens G3 enhanced taxonomic richness (ACE, Chao1) and evenness (Shannon, Simpson) in the rhizosphere bacterial community, leading to a significantly greater relative abundance of several bacterial genera: Pseudomonas, Methylophaga, Enhygromyxa, Desulfuromonas, and Devosia. These shifts in the microbial community composition suggest a potential restructuring of functional profiles, possibly enhancing processes beneficial to plant salt tolerance, such as ion homeostasis and stress mitigation: the biosynthesis of cofactors and secondary metabolites; bacterial secretion and two-component systems; porphyrin metabolism; flagellar assembly; biofilm formation; and bacterial chemotaxis. Redundancy analysis revealed positive correlations between microbial composition at both the phylum and genus levels and the activity of stress resistance enzymes after treatment with Pseudomonas fluorescens. This study provides important theoretical foundations and microbial resources for utilizing microbial community regulation in saline–alkali soil bioremediation. Full article
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17 pages, 7590 KB  
Article
Identification of the GST Gene Family in Reaumuria soongorica and Its Response to Drought Stress
by Jun Zhao, Liying Ma, Weibo Du, Qianwen Song, Luna Xing, Wei Chen, Qingyan Zhao, Chunlei Zhen and Songsong Lu
Biology 2026, 15(8), 660; https://doi.org/10.3390/biology15080660 - 21 Apr 2026
Viewed by 423
Abstract
This study conducted a genome-wide identification and functional analysis of the glutathione S-transferase (GST) gene family in the xerophytic desert shrub Reaumuria soongorica. A total of 67 GST genes were identified, classified into seven subfamilies, including Phi and Tau, with [...] Read more.
This study conducted a genome-wide identification and functional analysis of the glutathione S-transferase (GST) gene family in the xerophytic desert shrub Reaumuria soongorica. A total of 67 GST genes were identified, classified into seven subfamilies, including Phi and Tau, with family expansion primarily attributed to small-scale duplication events. The findings revealed that ResoGST52, a member of the Tau subfamily, serves as a core gene in drought response, exhibiting significant upregulation of 2.40-fold in leaves and 9.01-fold in roots under drought stress. Mechanistic investigations indicated that the expression of ResoGST52 is likely directly regulated by the transcription factor ResoDof17, with specific hydrogen bonding interactions identified between the two. Co-expression network analysis further demonstrated that ResoGST52 cooperates with key pathways such as plant hormone signaling, MAPK cascades, and glutathione metabolism to collectively respond to drought stress. Notably, evolutionary analysis revealed that ResoGST52 has undergone positive selection, with three positively selected sites identified. Among these, the p.Ala115Ser mutation increases the volume of the protein’s active site pocket, while the remaining mutations enhance surface hydrophobicity, thereby improving protein stability and catalytic efficiency under extreme drought conditions. In summary, this study not only systematically identifies the GST gene family in R. soongorica but also elucidates the central role of ResoGST52 in drought adaptation through multiple layers—from transcriptional regulation and co-expression networks to protein structural adaptive evolution—providing valuable candidate genes and theoretical insights for genetic improvement of drought tolerance in crops. Full article
(This article belongs to the Special Issue Adaptation Mechanisms of Forest Trees to Abiotic Stress (2nd Edition))
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23 pages, 23756 KB  
Article
Geochemistry and Ore Genesis of the Huoyanshan Cu-Zn Polymetallic Deposit, North Qilian Orogenic Belt, China: Constraints from Trace Element Compositions and Sulfur Isotopes
by Zaijia Zhang, Xiaogang Guo, Peiqing Hu, Bo Mai and Zhuang Wu
Minerals 2026, 16(4), 421; https://doi.org/10.3390/min16040421 - 19 Apr 2026
Viewed by 200
Abstract
The Huoyanshan Cu-Zn volcanogenic massive sulfide (VMS) deposit, located in the North Qilian Orogenic Belt, China, is of significant economic importance. This study provides new constraints on the ore-forming processes through high-resolution in situ trace element and sulfur isotope analyses of pyrite and [...] Read more.
The Huoyanshan Cu-Zn volcanogenic massive sulfide (VMS) deposit, located in the North Qilian Orogenic Belt, China, is of significant economic importance. This study provides new constraints on the ore-forming processes through high-resolution in situ trace element and sulfur isotope analyses of pyrite and sphalerite using LA-(MC)-ICP-MS. Petrographic and geochemical investigations identified three distinct generations of pyrite (Py l to Py III). Early-stage Py I and Py II are characterized by high trace element contents (Au, As, Bi, Cu, Pb), elevated Co/Ni ratios (>1–10), and enriched δ34S values (+4.98‰ to +7.47‰). These signatures indicate precipitation from high-temperature, reduced magmatic–hydrothermal fluids influenced by thermochemical sulfate reduction (TSR). Late-stage Py IIl exhibits markedly lower Co/Ni ratios (<0.1) and lighter δ34S values (+3.72‰ to 3.89‰). This geochemical shift reflects a transition toward a cooler, more oxidized environment driven by the incursion and mixing of ambient seawater as the hydrothermal system waned. Trace element geochemistry of sphalerite reveals an average crystallization temperature of 265.8 °C (derived from the “GGIMFis” geothermometer), consistent with fluid inclusion data and representing a thermal “snapshot” of the waning hydrothermal stage. Systematic discriminant analysis using Ga/In, Ge/In, and Co-Ni-As systematics further confirms a strong magmatic–hydrothermal affiliation. Full article
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32 pages, 19848 KB  
Article
Impacts of Land-Use Change on the Spatiotemporal Dynamics and Driving Mechanisms of Ecosystem Services in Arid and Semi-Arid Regions: A Case Study of Gansu Province, China
by Zhuanghui Duan, Xiyun Wang, Xianglong Tang, Chenyu Lu and Shuangqing Sheng
Land 2026, 15(4), 668; https://doi.org/10.3390/land15040668 - 18 Apr 2026
Viewed by 322
Abstract
The spatiotemporal evolution of ecosystem services and the elucidation of their driving mechanisms constitute a central scientific issue in territorial spatial optimization and regional sustainable development. Taking Gansu Province, a core area of the ecological security barrier in northwestern China, as the study [...] Read more.
The spatiotemporal evolution of ecosystem services and the elucidation of their driving mechanisms constitute a central scientific issue in territorial spatial optimization and regional sustainable development. Taking Gansu Province, a core area of the ecological security barrier in northwestern China, as the study area, this study integrates land-use, natural geographic, and socioeconomic data from 2000 to 2020. Using a land-use transfer matrix, the InVEST model, the Geographical Detector, and the PLUS model, we constructed a comprehensive analytical framework that combines historical evolution analysis, spatial differentiation identification, and multi-scenario simulation and prediction. The framework was used to systematically reveal the spatiotemporal dynamics of four core ecosystem services, namely carbon storage (CS), water yield (WY), habitat quality (HQ), and soil retention service (SDR), and to analyze their natural and socioeconomic driving mechanisms, while also simulating land-use change and ecosystem-service responses under the natural development, ecological protection, and urban expansion scenarios in 2030. The results show that, from 2000 to 2020, land use in Gansu Province was dominated by grassland (average proportion: 33.34%) and unused land (average proportion: 41.35%). Urban land expanded from 660.52 km2 to 2227.36 km2, with its share increasing from 0.15% to 0.50%, mainly through the conversion of cropland and grassland. Ecosystem services exhibited marked spatial differentiation: CS increased from east to west; WY showed an increasing pattern from northwest to southeast; HQ was lower in the central and southeastern regions and higher in the western and southern regions; and SDR was dominated by low-value areas in the northwest (average proportion: 84.81%). Driving-mechanism analysis indicated that slope was the core natural factor affecting CS, HQ, and SDR (q = 0.18–0.45), while mean annual precipitation dominated the variation in WY (q = 0.31–0.35). The influence of socioeconomic factors such as GDP increased gradually over time, showing an evolutionary trend from natural dominance to coordinated natural–socioeconomic regulation. Multi-scenario simulation further showed that, under the ecological protection scenario, grassland area increased significantly (+0.60%), the proportions of medium-value CS zones and high-value WY zones increased, and ecosystem services were optimized overall; under the urban expansion scenario, cropland and urban land expanded (+0.87% and +0.23%, respectively), imposing potential pressure on part of the ecosystem-service functions. These findings provide a scientific basis for optimizing territorial spatial planning, strengthening the ecological security barrier, and promoting regional sustainable development in Gansu Province. The methodological framework also offers a broadly applicable reference for ecologically sensitive arid and semi-arid regions in northwestern China. Full article
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14 pages, 2765 KB  
Article
Spectral Phase Control in Dissociation Dynamics of HD+ by Strong Laser Fields
by Tong Cheng, Wen-Quan Jing, Jin-Xu Du, Zeng-Qiang Yang, Zhi-Hong Jiao, Guo-Li Wang and Song-Feng Zhao
Photonics 2026, 13(4), 383; https://doi.org/10.3390/photonics13040383 - 16 Apr 2026
Viewed by 248
Abstract
Achieving selective cleavage of specific chemical bonds using ultrafast laser pulses remains a central challenge in ultrafast strong-field molecular physics. Here, we theoretically investigate the coherent control of strong-field dissociation of the heteronuclear molecular ion HD+ initially prepared in vibrationally excited states [...] Read more.
Achieving selective cleavage of specific chemical bonds using ultrafast laser pulses remains a central challenge in ultrafast strong-field molecular physics. Here, we theoretically investigate the coherent control of strong-field dissociation of the heteronuclear molecular ion HD+ initially prepared in vibrationally excited states driven by an ultrashort pulse with a quadratic spectral phase. Our results reveal a pronounced sensitivity of both the total dissociation probability and the branching ratio (H+ + D vs. H + D+) to the chirp rate of the laser pulse. To uncover the underlying physical mechanism, we analyze the population dynamics in the coupled 1sσ and 2pσ electronic states and identify pronounced Rabi oscillations arising from the coherent interplay between multiphoton excitation and field-induced stimulated emission. By tuning the laser chirp rate, these oscillations can be suppressed via quantum interference, thereby reshaping the dissociation dynamics and significantly enhancing the dissociation probability of the H + D+ channel. These findings demonstrate that spectral-phase engineering provides a robust and versatile strategy for selective control of branching ratios in strong-field molecular dissociation. Full article
(This article belongs to the Special Issue Laser-Driven Ultrafast Dynamics and Imaging in Atoms and Molecules)
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