Search Results (2,368)

Traffic variability along heavily congested signalised urban corridors undermines roadway safety, reduces energy efficiency, weakens operational reliability, and can hinder emergency response. Although many simulation-based studies have examined the impacts of Autonomous Vehicles (AVs), relatively few have combined high-resolution congestion observations with link-level microscopic calibration in a real urban network, particularly when evaluating implications for emergency mobility. This study develops and calibrates a microscopic Aimsun traffic simulation model for the Atakum district of Samsun, Türkiye, using a 10 min Google Traffic Index (GTI) observation stream converted into a four-level ordinal congestion scale. The calibration process began with an origin–destination (OD) matrix derived from 2020 traffic counts and was refined through link-level GTI synchronization, iterative OD scaling on mismatched corridors, and signal retiming at key intersections. GTI was validated as an ordinal congestion proxy through both categorical agreement and volumetric consistency, achieving 83% class agreement and GEH values below 5 for more than 90% of links. Five AV penetration scenarios (0%, 25%, 50%, 75%, and 100%) were simulated under peak-hour conditions. Network performance was evaluated using delay, stop time, mean speed, throughput, missed turns, and total journey time, while emergency mobility was assessed along a representative ambulance corridor on Atatürk Boulevard using seconds per kilometre. The results indicate that increasing AV penetration improves flow stability more clearly than nominal capacity. Mean speed increased from 36.2 to 39.2 km/h, delay and stop time declined steadily, and throughput remained nearly constant at 22.2–22.5 thousand vehicles/h. Along the ambulance corridor, travel time improved by 11.5%, from 112.4 to 99.4 s/km, between the baseline and full automation scenarios. These findings provide scenario-based evidence that, within a calibrated signalised urban network, increasing AV penetration can enhance operational stability and emergency response efficiency. More broadly, the study demonstrates the practical value of integrating GTI-based congestion observations with microscopic simulation for AV impact assessment in real urban networks. Full article

Rain-induced shallow landslides persistently disrupt Nepal’s mountain roads, frequently leading to fatalities, transport disruptions, and economic losses. This study develops physically validated, site-specific rainfall thresholds for the landslide-prone Kanti National Roadway (H37) by integrating empirical intensity–duration (I-D) analysis, antecedent rainfall metrics, and satellite-derived soil moisture data. Using 35 years of rainfall records (1990–2024) and 59 field-verified landslides (2017–2024), we derived a localized I-D threshold: I = 19.37 × D^−0.6215 (I: rainfall intensity in mm/h; D: duration in hours), effective for durations of 48–308 h, encompassing short intense storms and prolonged moderate rainfall. The Cumulative Antecedent Rainfall (CAR) method associated most failures with 3-day totals, while the Antecedent Precipitation Index (API) showed superior performance, with a 10-day threshold of 77 mm capturing all events. For physical validation, NASA’s SMAP Level-4 root-zone (0–100 cm) soil moisture data revealed a 1-day lag in response to rainfall; after adjustment, trends matched API saturation predictions and identified an inverse rainfall–moisture pattern before the 11 August 2019 landslide, indicating a potential instability precursor. This integration enhances predictive accuracy, bolsters mechanistic understanding of landslide hazards, and offers a scalable, cost-effective early-warning framework for data-scarce mountain regions, aiding climate-resilient infrastructure in regions with intensifying rainfall extremes. Full article

Purpose: We hypothesised that cognition following consumption of an isomaltulose beverage would be comparable to that of an isoenergetic sucrose-based beverage, but the latter would attenuate post-ingestive metabolic responses. Methods: Thirty adults (15 males, 15 females) aged 21–44 years completed three experimental sessions, following at least 3 h fasting. Plasma insulin and glucose were measured in arterialised capillary blood 30 min after beverage consumption. Cognitive functions were assessed 45 min after beverage consumption using a computerised test battery; the primary cognitive performance outcome was a composite neurocognitive index score. Subjective symptoms were measured using questionnaires. Data are presented as the mean [95% confidence interval]. Results: Circulating glucose was greater after ingesting sucrose compared to isomaltulose and placebo beverages (sucrose: 7.3 [6.9, 7.7] mmol·L⁻¹; isomaltulose: 6.3 [6.1, 6.6] mmol·L⁻¹; and placebo: 5.3 [5.2, 5.4] mmol·L⁻¹). Insulin rose to a greater degree with sucrose compared to isomaltulose (mean difference = 8.5 [2.4, 14.6] µU·mL⁻¹, p = 0.005). Non-inferiority was shown between isomaltulose and sucrose for the composite neurocognitive index score (isomaltulose mean score = 0.931 [−2.3, 4.2]; sucrose mean score = 0.414 [−2.6, 3.5]). However, performance with the sucrose and placebo beverages was similar, limiting broader interpretation. The sensation of postprandial tiredness for isomaltulose was non-inferior to sucrose (isomaltulose mean score = −3.8 [−15.8, 8.2]; sucrose mean score = 0.1 [−10.9, 11.1]). Conclusions: A commercial stimulant beverage with isomaltulose as the energy substrate elicits substantial reductions in glycaemic and insulinaemic responses compared with an isoenergetic sucrose-based beverage, without compromising cognitive performance. Full article

Background: Hypertension (HT) and atrial fibrillation (AF) are frequently coexisting conditions, with inflammation being a crucial pathophysiological mechanism shared by both. The systemic immune-inflammation index (SII), a newly introduced combined inflammatory marker, includes the parameters of platelets, neutrophils, and lymphocytes. Current literature lacks studies investigating the relationship between SII and newly developed AF in patients with controlled HT. Therefore, this study aimed to explore the association of SII with AF in hypertensive patients on anti-hypertensive therapy. Methods: This retrospective case–control study included 68 hypertensive patients with controlled blood pressure who underwent 24 h Holter monitoring. Patients were divided into two groups based on the presence of atrial fibrillation (AF-positive, n = 32; AF-negative, n = 36). SII values were calculated from complete blood counts. Demographic, clinical, and laboratory characteristics were compared between groups. Correlation analysis was performed to assess associations of SII with clinical parameters. Multivariable logistic regression identified independent predictors of AF. Optimal cut-off values for systemic immune-inflammation index and age were determined through receiver operating characteristic analysis. Results: Patients with AF were significantly older than those without AF (p < 0.01). The systemic immune-inflammation index was significantly higher in patients with atrial fibrillation (p = 0.02). Patients with AF also had higher heart rates (p = 0.04) and creatinine levels (p < 0.01). SII showed a moderate positive correlation with C-reactive protein (CRP) (r = 0.393, p < 0.01) and a weak correlation with heart rate (r = 0.251, p = 0.039). In multivariable analysis, SII (OR: 1.024, p = 0.04) and age (OR: 1.130, p < 0.01) was independently associated with AF. Receiver operating characteristic analysis determined an SII cut-off of 483.0 with a sensitivity of 53.1% and specificity of 52.8%. The age cut-off was found to be 63 years with sensitivity and specificity being 62.5% and 66.7%, respectively. Conclusions: The systemic immune-inflammation index was significantly elevated in newly diagnosed AF patients with well-controlled hypertension and was an independent predictor of AF. It is a simple, readily available biomarker that may assist in identifying hypertensive patients at high risk for the development of AF. These results should be validated in future studies, and the role of inflammation in the pathogenesis of atrial fibrillation should be further explored. Full article

This study used Global Navigation Satellite System (GNSS) observations from the China Crustal Movement Observation Network (CMONOC) and the Kunming Continuously Operating Reference Station (KMCORS) network to investigate ionospheric response characteristics over China during the geomagnetic storm of 4–6 November 2023, and to assess their impacts on CORS-based real-time kinematic (RTK) positioning performance in the low-latitude Kunming region. A quantitative assessment was conducted by integrating regional two-dimensional dTEC (%) maps over China, BeiDou Navigation Satellite System (BDS) Geostationary Earth Orbit (GEO) total electron content (TEC), the rate of TEC index (ROTI), and RTK positioning solutions to evaluate ionospheric disturbances, irregularity activity, and associated degradation in positioning performance. Results indicate that, during geomagnetic storms, ionospheric responses over China exhibit pronounced phase-dependent and latitudinal variations. During the second geomagnetic storm on 5–6 November, positive responses were dominant at mid-to-high latitudes, whereas alternating positive and negative responses were observed at low latitudes. During the recovery phase, the Kunming region successively experienced a positive ionospheric storm lasting approximately 10 h, followed by a negative ionospheric storm lasting about 7 h, with relative TEC variations reaching a maximum of approximately 90%. The GEO TEC time series was consistent with the temporal evolution of the two-dimensional dTEC (%), while ROTI increased markedly during the disturbance enhancement period (21:00 UT on 5 November to 07:00 UT on 6 November 2023). During periods of enhanced ionospheric response and irregularities, RTK positioning performance was observed to deteriorate markedly. The fixed-solution rate at medium-to-long baseline stations decreased from nearly 100% to close to 0%, accompanied by an increase in vertical positioning errors to approximately 20 cm, whereas short-baseline stations were only minimally affected. These results indicate that ionospheric disturbances during geomagnetic storms exert a pronounced impact on CORS-based RTK positioning services in the Kunming region, with the magnitude of this impact being closely related to baseline length. Full article

Glycolic acid (GA), a widely used alpha-hydroxy acid in cosmetic formulations, promotes exfoliation and stimulates fibroblasts in the dermis to synthesize collagen. However, its hydrophilic nature limits penetration through the stratum corneum, reducing its overall efficacy. This study aimed to develop and optimize an ethanol-based niosomal system to enhance GA skin delivery and formulation stability for cosmetic applications. Brij 97 combined with cholesterol at a 1:1 ratio and 10% ethanol produced the optimal formulation. Blank vesicles exhibited a mean vesicle size of 170.53 ± 5.05 nm and a zeta potential of −37.77 ± 2.21 mV, indicating favorable colloidal stability. Incorporation of 10% GA resulted in vesicles with a mean size of 176.93 ± 1.51 nm, a polydispersity index of 0.12 ± 0.02, and an entrapment efficiency of 75.48 ± 0.21%. In vitro permeation studies using Strat-M^® membranes demonstrated significantly higher cumulative skin penetration (49.56 ± 8.95 mg/cm²) and sustained release over 24 h compared with a conventional GA solution. Stability testing under heating–cooling cycles and storage at 4 °C showed slight increases in vesicle size while maintaining homogeneity (polydispersity index (PDI) < 0.3). These findings highlight ethanol-based niosomes as an effective strategy for enhancing GA cosmetic performance. Full article

The African catfish (Clarias gariepinus) is an important aquaculture species in the Philippines, but it exhibits reproductive dysfunctions in captivity due to the absence of natural spawning cues and culture-induced stress. In hatcheries, sperm collection often requires sacrificing male breeders, limiting breeding program applications, and threatening genetic diversity. This study evaluated and optimized hormonal induction protocols to enhance spermiation and fertilization success in male C. gariepinus. Two experimental trials were conducted under controlled hatchery conditions. In Trial 1, human chorionic gonadotropin combined with a dopamine antagonist (hCG + DA; 5000 IU·kg⁻¹ BW + 5 mg·kg⁻¹ BW) and Ovaprim (1.0 mL·kg⁻¹ BW) significantly increased circulating testosterone and estradiol levels, spermiation index, and fertilization outcomes (embryo viability, hatching, and larval survival). In Trial 2, medium to low doses of hCG + DA (3000–5000 IU·kg⁻¹ BW + 5 mg·kg⁻¹ BW) and Ovaprim (0.75–1.0 mL·kg⁻¹ BW) yielded the best performance in terms of hormone levels, spermiation index, and fertilizing capabilities. The presence of a dopamine antagonist (DA) likely contributed to the efficacy of the treatments, since dopamine suppresses the secretion of gonadotropin-releasing hormone (dopaminergic inhibition), and DA counteracts this effect, increasing LH and FSH levels necessary for gametogenesis. Taken together, these results highlight the potential of optimized hormonal protocols to mitigate reproductive dysfunctions in male C. gariepinus in aquaculture settings. Further studies should refine dose combinations, assess the long-term health of broodstock, and evaluate cost-effectiveness to support sustainable broodstock management and genetic conservation. Full article

Soil salinization is a major constraint on irrigated rice cultivation, mainly due to poor irrigation management and cropping in coastal areas. Seed priming is widely recognized as a cost-effective and practical approach to enhance early growth and improve tolerance to abiotic stresses, including salinity. This study evaluated the effects of seed priming of rice seeds from two cultivars, BRS Querência (Indica) and BRS 358 (Japonica), using aqueous carrot root extract at 0% (water), 25%, and 50% concentrations for 48 h. Seeds were sown in rhizotrons and exposed to 0, 75, or 150 mM NaCl. Morphological, physiological, and biochemical traits were evaluated at 21 days after sowing. Seed priming with carrot extract was associated with improved growth and physiological responses under salinity stress. Under 150 mM NaCl, primed seedlings showed approximately 40% higher chlorophyll index, 35% greater root volume, and 30% higher shoot dry mass compared to unprimed controls. The 25% extract concentration was particularly effective for BRS Querência, which showed enhanced root elongation and a higher nitrogen balance index. Activities of superoxide dismutase, ascorbate peroxidase, and catalase increased by 45–70%, while hydrogen peroxide and malondialdehyde levels decreased by approximately 50%, suggesting enhanced antioxidant responses and improved redox balance. Anthocyanin accumulation also increased in specific cultivar–treatment combinations, suggesting a potential effect on secondary metabolism and antioxidant pathways. Overall, carrot-based seed priming was associated with improved seedling performance, pigment stability, and regulation of oxidative stress under saline conditions. These results suggest that carrot-based seed priming may improve physiological performance under salinity stress. Full article

This study aimed to evaluate the potential of Lonicera japonica Flos (LJF) as an alternative agent against Eimeria tenella (E. tenella) in chickens and to conduct phytochemical analysis to obtain compositional insights. Seventy-two one-day-old chickens were allocated to six groups and fed diets supplemented with LJF powder (LJFp) at three concentrations (LJFp-L, LJFp-M, and LJFp-H) or maduramicin (MDM) or two non-supplemented control diets, namely, an infected unmedicated control (IUC) and an uninfected unmedicated control (UUC). Dietary treatments were initiated at chick arrival (Day 0) and continued for 28 days. At 21 days of age, all groups except the UUC group were orally challenged with a field isolate of E. tenella (PT-Te003; 2.0 × 10⁴ oocysts/bird). Anticoccidial efficacy was assessed using the lesion score (LS), oocysts per gram of feces (OPG), relative body weight gain (rBWG), and anticoccidial index (ACI). The results demonstrated that all LJFp treatment groups had significantly reduced cecal OPG and LS (all LJFp treatments: p < 0.05 vs. IUC), indicating the effective suppression of E. tenella replication and intestinal damage. Regarding growth performance, the rBWG values of the LJFp-L and LJFp-M groups were the highest and comparable to those of the UUC group, showing no significant differences. In contrast, the LJFp-H and MDM groups exhibited significantly lower values (p < 0.05). Based on ACI evaluation, all LJFp-treated groups exhibited moderate to partial efficacy (LJFp-L > LJFp-M > LJFp-H), while MDM showed limited effectiveness. A gas chromatography–mass spectrometry (GC-MS) analysis of the LJFp ethanol extract revealed 15 essential oils, 10 organic acids, and three other compound classes, several of which have been associated with anticoccidial activity. Overall, the in vivo results suggest that LJF may exert potential anticoccidial effects against a field isolate of E. tenella. Phytochemical analysis provided preliminary compositional insights, and further studies are warranted to optimize extraction methods and evaluate efficacy at lower concentrations under additional in vitro and in vivo conditions. However, the current evidence remains insufficient to determine whether the field isolate exhibits reduced sensitivity to commercially available anticoccidial drugs, and additional studies are needed to clarify this issue. Full article

This study aimed to assess the effect of concentrate supplementation frequency on the nutrient intake, performance, carcass characteristics, and tissue composition of sheep for meat raised on Panicum maximum cv. Massai pasture. A group of 36 sheep of the Santa Inês breed (18 males and 18 females) with a mean initial weight of 17.0 ± 1.5 kg and mean age of 90 ± 10 days were assigned to three treatments: supplementation every 24 h, every 48 h, and every 72 h. The formulation comprised ground maize kernels (70%), soybean meal (25%), mineral mix (2.5%), urea (1.8%), table salt (0.5%), and ammonium sulfate (0.2%). The females reached a higher final weight than the males (p < 0.05). Dry matter intake did not differ among the treatment groups (p > 0.05), but the sheep supplemented every 72 h showed greater pasture and neutral detergent fiber (NDF) intake, while those supplemented every 24 h and 48 h consumed more concentrate and had higher total weight gain (7.19 and 7.10 × 5.68 kg) (p < 0.05). The same behavior was found for carcass and meat cut weights and for leg and carcass compacity indexes, but carcass and cut yields were not impacted by supplementation strategy. Supplementation on alternate days, i.e., every 48 h, maintains the performance and productive efficiency levels in sheep kept on Massai grass pasture compared to those supplemented daily, but providing intermittent supplementation with intervals above 48 h is not recommended as a nutritional strategy. Full article

Background/Objectives: The renal resistive index (RRI) has emerged as an early marker of renal vascular resistance. The purpose of this study was to investigate the association between RRI on intensive care unit (ICU) admission and the development of acute kidney injury (AKI) in a general ICU population, and to assess its predictive accuracy. Methods: This prospective observational study was conducted in a multidisciplinary ICU. Consecutive mechanically ventilated adults were enrolled; RRI was measured within 24 h of admission after hemodynamic stabilization. AKI was defined by Kidney Disease: Improving Global Outcomes (KDIGO) criteria within seven days. Multivariable regression, receiver operating characteristic (ROC), reclassification, and mediation analyses were performed. Results: A total of 181 patients were included. AKI occurred in 36%. Median RRI was 0.73 (0.65–0.80). RRI correlated with age, acute physiology and chronic health evaluation (APACHE) II and sequential organ failure assessment (SOFA) scores, lactate, and glomerular filtration rate (GFR) (all p < 0.001). In multivariable analysis, RRI was the only independent predictor of AKI (OR 2.86 per 0.05 increase, 95% CI 1.64–4.98, p = 0.001). It was also associated with an increased likelihood of presenting with a more severe AKI stage. RRI showed high discriminative ability (AUC = 0.89, 95% CI 0.84–0.94); the optimal cut-off was 0.77 (sensitivity 0.83, specificity 0.82). Adding RRI to a clinical model improved prediction (ΔAUC p = 0.049; net reclassification index (NRI) = 0.52, p < 0.001). Mediation analyses showed that RRI significantly mediated the effects of hypertension and low baseline GFR on AKI risk. Subgroup analyses confirmed consistent predictive performance across age, lactate, and sepsis categories. Conclusions: RRI is an independent early predictor of AKI and its severity, as well as a mediator of both hypertension and low GFR, regarding their effect on AKI development in ICU patients. RRI could serve as an early bedside marker of renal perfusion impairment in critically ill patients, guiding strategies aimed at reducing the risk of AKI. Full article

Autoimmune gastritis (AIG) in its advanced atrophic stage is characterized by reduced acid secretion, dysbiosis, and gastric cancer (GC) risk. Swallowed oral bacteria surviving in increased intragastric pH may play a carcinogenic role. Oral microbiota was linked to increased GC risk. In AIG, the concomitant assessment of oral and gastric microbiota has not yet been performed. This study aimed to investigate the oral and gastric microbiota in AIG patients to clarify the role of oral bacteria in gastric dysbiosis. A case–control study on n = 20 histologically diagnosed AIG patients and n = 20 controls without AIG is conducted. Saliva samples were obtained from subjects who were fasting and without toothbrushing. Within 1 h, gastroscopy with biopsies (for histopathology and DNA extraction) was performed. Saliva (n = 40) and biopsy (n = 40) samples were frozen at −20 °C. DNA was extracted and prepared; paired-end sequencing was performed (IlluminaMiSeq-sequencer, San Diego, CA, USA). Bacterial abundance in biopsies was higher in AIG than in controls (p = 0.06), but was not different in the saliva (p = 0.54) samples. In biopsies, AIG showed a lower Shannon-Index than controls (p = 0.001). In saliva studies, AIG showed a higher Shannon-Index than controls (p = 0.0). In biopsies, Streptococcus oralis, Fusobacterium pseudoperiodonticum, Veillonella rogosae, and Gemella sanguinis were more frequent in AIG (p < 0.03). The most abundantly shared taxa between saliva and biopsy were S. oralis and Prevotella histicola; Gemella sanguinis, Fusobacterium pseudoperidonticum, and Veillonella rogosae were shared in AIG patients only. This study confirmed gastric dysbiosis in AIG. Oral taxa were more commonly associated with AIG and shared between the mouth and the stomach. In AIG, the oral microbiota is associated with gastric dysbiosis, highlighting the importance of oral eubiosis in patients with impaired gastric acid secretion. Full article

Drying agents used in the process of spray drying whey enhance the quality of whey powders and may consequently improve the properties of yogurts to which these powders are added. This study examined the quality characteristics of yogurts enriched with whey powders using maltodextrin or gelatin as drying agents. The following yogurt variants were produced: a control (C) without whey powder; W with whey powder but without a drying agent; M with whey powder containing maltodextrin; and G with gelatin as a drying agent. Whey powders were added at a concentration of 1.5% (w/w). The samples were tested after 3, 6, 10 and 14 days of refrigerated storage (4 ± 1 °C), and analyses were performed in 3–6 repetitions. Enrichment of milk with powdered whey had a noticeable impact on the quality characteristics of yogurts. Fortified yogurts exhibited a significantly higher total solids content (11.43–12.22% in the control; 12.45–13.33% in W, G and M) and higher acetaldehyde concentration (0.31–1.11 ppm in the control; 0.44–1.82 ppm in W, G and M) than the control. Titratable acidity and pH varied among samples. However, the syneresis index was lower in the control yogurt (39.09–46.74%) than in yogurts with powdered whey (53.71–58.48%). Significant differences were observed in color parameters (L, a*, b*, WI, YI). The texture properties (firmness, consistency, cohesiveness, index of viscosity) of samples with whey powders had higher values than the control. In sensory evaluation, yogurts with whey powders obtained high scores. The study demonstrated that the use of maltodextrin or gelatin not only supports the process of spray drying whey but also alters the characteristics of the resulting yogurts. The impact of the study comes from the fact that it bridges the gap between whey processing (spray drying) and the formulation of the final product. Full article

To evaluate the behavior of industrial equipment from a corrosion point of view, it is mandatory to consider both the material that equipment is made from and the working conditions such as temperature, pH, and the existing microorganisms in the working environment. Our studies regarding ecotoxicological monitoring of biological suspensions Diatomee, Saccharomyces, and Spirulina (DSS) are focused on three directions: (1) the evolution of chemical and biological parameters of the reaction environment (pH, conductivity, TDS, DO, OD), the kinetics of DSS microorganisms’ growing curve; (2) the analysis of biofilm forming on the exposed metallic surface and (3) the analysis of corrosion degree (phenomena) of tested metals in five media, by using the corrosion indices: volumetric index, gravimetric index, and penetration index. The viability of microorganisms in the presence of aluminum, zinc, and iron shows the following sequence: Al_Diat > Fe_Diat > Zn_Diat > Al_Spir > Zn_Spir > Al_Sach > Zn_Sach > Fe_Spir > Fe_Sach. The development of biofilms on the surface of metal plates followed the sequence outlined below: Al_Diat > Fe_Diat > Zn_Diat > Fe_Spir > Zn_Sach > Fe_Sach > Al_Sach > Zn_Spir > Al_Spir. Iron exhibits the most favorable performance, displaying a very low Ip value across all tested environments, including salt water. Aluminum demonstrates sensitivity to specific biological environments, with the highest degree of corrosion observed in Spirulina, indicating that not all biological environments confer protection to aluminum. Diatoms and Saccharomyces suspensions exert an inhibitory effect on corrosion. Zinc is the most susceptible metal, experiencing the greatest corrosion in Spirulina, followed by salt water, while biological environments only partially mitigate the corrosion rate. Full article

Background/Objective: Buccal delivery offers a potential route to circumvent gastrointestinal degradation and hepatic first-pass metabolism, but hydrophilic peptides typically exhibit limited mucosal permeation. Nanostructured lipid carriers (NLCs) have been proposed as delivery platforms capable of modulating interfacial interactions and improving mucosal transport. This study aimed to quantitatively evaluate the ex vivo buccal permeation of angiotensin II (Ang II), used as a hydrophilic peptide model, when associated with NLCs compared with free peptide under matched Franz diffusion cell conditions. Methods: Ang II-associated NLCs were prepared by melt emulsification combined with a low-energy injection technique. Particle size, polydispersity index, and zeta potential were determined by dynamic light scattering and laser Doppler electrophoresis. Association efficiency and drug loading were quantified by indirect spectrofluorometric analysis. Ex vivo permeation studies were conducted using porcine buccal mucosa mounted in Franz diffusion cells, and cumulative permeation, steady-state flux, and apparent permeability coefficients were calculated. Results: The NLCs exhibited nanometric size, moderate polydispersity, and association efficiency above 80%, and remained colloidally stable at 4 °C for 28 days. In ex vivo experiments, Ang II-associated NLCs showed measurable cumulative permeation, reaching approximately 9% after 2 h, whereas free Ang II was not detected in the receptor compartment under the tested conditions. Conclusions: This work provides a quantitative ex vivo buccal transport comparison of a hydrophilic peptide model delivered as NLC-associated versus free peptide under matched Franz cell conditions. The findings support further investigation of NLC-based approaches for buccal delivery of vasoactive peptides and provide a rational basis for future in vivo evaluation of mucosal delivery performance and systemic exposure. Full article