Search Results (44,813)

Background: Fascicular turnover flap (FTF) is a donor-sparing option for segmental facial nerve repair. This study compared autologous nerve grafting with proximally based and distally based FTF in a rat facial nerve model. Methods: Adult male Wistar rats were randomized to autograft, proximal FTF, or distal FTF (n = 8 per group). A single additional animal with an untreated defect served as a qualitative histological reference. The prespecified primary endpoint was whisker motion amplitude at week 8; the secondary endpoints were central section histomorphometry (nerve tissue area, µm²) and variability metrics (IQR, SD, and coefficient of variation) as measures of reproducibility. Non-parametric tests (Kruskal–Wallis; Mann–Whitney U) were used; pairwise functional comparisons were Holm-corrected; and effect sizes were expressed as Cliff’s δ. Results: At week 8, the overall functional comparison was significant (Kruskal–Wallis p = 0.047), but no pairwise contrast remained significant after Holm correction. Functional recovery was highest in the autograft group, followed by proximal FTF and distal FTF. Both FTF groups showed lower inter-animal variability than autograft for the week-8 functional endpoint, with the distal FTF showing the lowest dispersion. Central section nerve area comparisons did not reach global significance; effect sizes and descriptive statistics favored autograft, and a single unadjusted pairwise contrast (autograft > proximal FTF) should be interpreted cautiously. Conclusions: Both FTF configurations achieved measurable functional and structural regeneration while avoiding an additional free donor nerve graft. Within an 8-week window, autograft remained the benchmark. Between FTF variants, distal FTF produced more stable functional outcomes, but this did not translate into superior functional recovery. Confirmation in larger, balanced cohorts with longer follow-up and vascular/neural labeling is warranted. Full article

Post-blast operations in tunnel construction represent a critical bottleneck due to mandatory downtime and hazardous environmental conditions. This study addresses these challenges by developing and validating an integrated cyber–physical architecture that coordinates an autonomous Unmanned Aerial Vehicle (UAV) and an Autonomous Wheel Loader (AWL) under the supervision of a Digital Twin acting as central operational digital interface. Specifically, this technology was designed to access the tunnel, evaluate post-blasting conditions, and initiate operations during mandatory exclusion periods for personnel. The system was validated in a realistic, Global Navigation Satellite System (GNSS)-denied tunnel environment emulating post-detonation visibility constraints. The results demonstrate that the aerial agent successfully navigated and mapped the excavation front in less than 8 min, establishing a shared coordinate system for the ground machinery. Through this collaborative workflow, the autonomous deployment enabled operations to commence 50% to 80% earlier than conventional manual procedures. Furthermore, the system reduced daily operational time by approximately 8%, with an estimated return on financial investment between one and seven months. Overall, the proposed framework eliminates human exposure during high-risk inspections and transforms the fragmented excavation cycle into a continuous, data-driven process. Full article

This study investigates the fabrication of microcapsules using Nigella sativa (N.S.) oil as the core and alginate as the shell material. The N.S. oil microcapsules were prepared using the sol–gel method with different oil concentrations. The microcapsules were applied to the cotton fabric by the pad–dry–cure method, and their attachment was evidenced by scanning electron microscopy (SEM). Air permeability measurements were conducted for all developed samples, revealing that the sample with 8 g loading of N.S. oil and 4.5 g alginate exhibited a 43% reduction compared to the pristine sample. To further investigate the comfort characteristics of the samples, the functionalized cotton samples were subjected to the water vapor permeability index test. The results yielded an index value of 90, indicating that the encapsulation process preserved the comfort characteristics of the samples. Among the samples, the specimen with an oil concentration of 8 mL displayed the maximum antibacterial performance, achieving a 90% reduction in colony-forming units (CFUs) following quantitative testing protocol. However, the qualitative antibacterial assessment indicates no clear zone of inhibition, but no bacterial growth was observed on the samples. Furthermore, the fabric incorporating the maximum loadings of N.S. oil and alginate capsules exhibited the maximum antioxidant activity of 86.5%. These results underscore the critical role of N.S. oil microcapsules in enhancing the antibacterial and antioxidant properties of cotton fabric, while also revealing a harmony between functional performance and comfort characteristics. Full article

Volumetric modulated arc therapy-computed tomography (VMAT-CT), which is the CT reconstructed using the portal images collected during VMAT, can potentially be an effective onsite imaging tool. The goal of this study was to propose an iterative reconstruction algorithm that can further improve the image quality of VMAT-CT and reduce the number of failed reconstructions. An iterative algorithm combining total variation (TV) with block-matching and 3D filtering (BM3D) was proposed, addressing the L1-L2 regularization problem using the split Bregman method. We collected portal images from 67 VMAT cases including 50 phantom and 17 real-patient cases. Both Feldkamp–Davis–Kress (FDK) and TV-BM3D iterative algorithms were used to reconstruct VMAT-CT using the collected images. The preprocessing methods developed by our group previously were also used in this study. A total of 48 out of 50 phantom cases and 15 out of 17 real-patient cases were successfully reconstructed using the iterative algorithm together with image preprocessing. In contrast, 39 phantom cases and 8 patient cases could be reconstructed using the original FDK algorithm, and 44 phantom cases and 11 patient cases could be reconstructed using the FDK algorithm together with preprocessing. Compared with the FDK algorithm, the TV-BM3D iterative algorithm significantly improved the image quality of VMAT-CT at all treatment sites. To the best of our knowledge, this study is the first to develop an iterative VMAT-CT reconstruction algorithm. It can be used to reconstruct CT images locally, and is superior to FDK-based algorithms in terms of the success rate and reconstructed image quality. This strongly supports the use of VMAT-CT as a promising imaging tool for treatment monitoring and adaptive radiotherapy. Full article

Critical minerals are central to industrial strategies in the Global South, but evidence on how such policies reshape domestic production is limited. This paper maps Indonesia’s nickel ecosystem before and after the 2014 export ban using input–output multipliers and labor intensity from the 2010, 2016, and 2020 input–output tables. We provide a descriptive account of nickel’s evolving economic trajectory during the downstreaming push. Three patterns stand out. Forward linkages declined from 16 to 8 and backward linkages moved from 75 to 73, suggesting a narrower structure with greater specialization in higher value, more capital-intensive activities. Output multipliers rose most in sectors that support the electric vehicle supply chain, including professional and technical services, machinery, fabricated metals, transport equipment, energy, and finance. In contrast, the labor multiplier fell from about 6514 to 3366 jobs per IDR 1 trillion of final demand, implying a higher value added alongside lower employment intensity. Overall, downstreaming appears to work through structural concentration and growth in complementary sectors rather than broad-based diversification. Complementary policies in skills, regional development, and energy infrastructure are therefore critical for inclusive industrial transformation. Full article

The rapid detection of organophosphorus (OP) compounds is crucial for safeguarding human health and ensuring food safety. This study presents a novel wearable electrochemical biosensor that integrates miniaturized screen-printed electrodes with wearable devices to achieve real-time, on-site OP detection. The biosensor was fabricated by constructing a screen-printed carbon electrode (SPCE) on a thermoplastic polyurethane (TPU) substrate, sequentially modified with graphene (GR), gold nanoparticles (AuNPs), and organophosphorus hydrolase (OPH), and finally encapsulated with Nafion. This SPCE/GR/AuNPs/OPH/Nafion configuration yields a highly flexible and portable device. The detection principle relies on the enzymatic hydrolysis of methyl paraoxon (MPOX) by OPH, generating p-nitrophenol (PNP), which is quantitatively measured via square wave voltammetry (SWV). The sensor exhibits a broad linear detection range (30–400 μM) with a strong linear correlation (R² = 0.995) and a low detection limit (0.321 μM). It demonstrates excellent selectivity against common interfering substances, including urea, sucrose, and various metal ions. Application to real-world samples such as cabbage and tap water yielded high recoveries (107.2% for cabbage and 101.2% for tap water), with relative standard deviations (RSDs) below 8%. Furthermore, the biosensor maintains robust flexibility and mechanical resilience, with less than 5% signal loss after 100 bending cycles, confirming its suitability for wearable applications and reliable operation under mechanical stress. This innovative, flexible electrochemical biosensor provides a powerful and reliable platform for rapid OP detection, particularly in complex testing environments. Full article

Knowledge sharing (KS) among healthcare professionals is essential for sustaining organisational learning and facilitating the transfer of expertise between experienced and less experienced professionals, thereby supporting workforce stability and retention in healthcare organisations (HCOs). However, despite its importance, high turnover among healthcare professionals remains a significant and persistent challenge in public HCOs, indicating a potential gap in understanding the mechanisms that support workforce stability. To address this gap, this study examines the interplay between work performance (WP), satisfaction with co-workers (CW), KS and turnover intention (TI) among healthcare professionals. Data from 220 respondents were analysed using Partial Least Squares Structural Equation Modelling (PLS-SEM) within the Input–Process–Output (IPO) framework. The results indicate that CW positively influences KS, while KS has a negative effect on TI, thereby reducing TI. In contrast, WP does not have a statistically significant effect on KS, nor does it indirectly influence TI through KS. Furthermore, although both WP and CW were hypothesised to be predictors of KS, only CW demonstrates a significant indirect effect on TI through KS. Grounded in Social Exchange Theory (SET) and the Knowledge-Based View (KBV), the results highlight the role of KS and interpersonal relationships in supporting sustainable human resource management (SHRM). Although sustainability-related dimensions were not directly measured, the results suggest potential implications for the Sustainable Development Goals (SDGs), particularly SDG 3, SDG 8, and SDG 9. Full article

Diabetic retinopathy (DR) is a major cause of adult blindness and is characterized by progressive retinal vascular dysfunction and pathological angiogenesis. To establish a DR model, streptozotocin (STZ) was intraperitoneally injected into rats. After 8 weeks, naïve placenta-derived mesenchymal stem cells (PD-MSCs) or PEDF-overexpressing PD-MSCs (PD-MSCs^PEDF) were intravitreally transplanted into the right eye for 4 weeks. Pathological neovascularization in DR is regulated by the balance between vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF). In diabetic retinas, increased VEGF and decreased PEDF expression were reversed following PD-MSC transplantation. Notably, PD-MSCs^PEDF treatment resulted in higher PEDF, and lower VEGF expression compared with naïve PD-MSCs, with similar expression patterns observed in the contralateral non-transplanted eyes. These findings indicate that engineering PD-MSCs^PEDF enhances anti-angiogenic activity by modulating VEGF and PEDF balance, thereby alleviating vascular damage in STZ-induced diabetic retinas. Full article

Background: Pre-eclampsia (PE) is a significant cause of maternal and perinatal morbidity and mortality globally and is characterized by impaired endothelial function and disturbances in coagulation pathways. The effects of Human Immunodeficiency Virus (HIV) on the immune and coagulation systems have been investigated during pregnancy, but there are few reports on anticoagulant factors in pregnant women who are infected with HIV and develop PE. This investigation compares plasma protein C levels in pregnant women with pre-eclampsia and those without pre-eclampsia, and compares the results based on their HIV status. Methods: A hospital-based cross-sectional study design was used for the current research, which was carried out at Charlotte Maxeke Johannesburg Academic Hospital, South Africa. A total of 83 pregnant women participated in the study and were categorized into one of four groups: normotensive HIV-negative (n = 36); normotensive HIV-positive (n = 18); pre-eclamptic HIV-negative (n = 21); and pre-eclamptic HIV-positive (n = 8). Data collected included demographic information and clinical characteristics that were abstracted from maternity records. Plasma protein C concentrations were determined by ELISA (enzyme-linked immunosorbent assay). Nonparametric statistical methods were used to compare the mean values of plasma protein C between each of the four groups, and significance was set at p < 0.05. Subgroup analyses, particularly for the pre-eclamptic HIV-positive group (n = 8), were considered exploratory due to small sample sizes. Results: As would be anticipated, both systolic and diastolic blood pressure values were significantly elevated in the pre-eclamptic group when compared to the normotensive control subjects (p < 0.0001). There were no statistically significant differences in plasma protein C concentration between the normotensive and pre-eclamptic groups, nor between the HIV-negative and HIV-positive groups. Similarly, there were no significant differences in plasma protein C concentration when comparing all four study groups (Kruskal–Wallis test p = 0.2295). Conclusions: Plasma protein C concentrations did not vary significantly according to the presence of pre-eclampsia or HIV status in this cohort. These findings suggest that protein C concentrations were not measurably altered between groups within this study population. However, due to the small sample size in key subgroups, these findings should be considered preliminary and interpreted with caution. Larger, adequately powered studies are required to further investigate potential associations between HIV infection, pre-eclampsia, and anticoagulant pathways during pregnancy. Full article

Background/Objectives: Rho-associated protein kinase inhibitors reduce intraocular pressure (IOP) by enhancing aqueous humor outflow through the trabecular meshwork–Schlemm’s canal pathway. However, it remains unclear whether the fixed-dose combination of ripasudil hydrochloride hydrate and brimonidine tartrate (GLAALPHA) enhances conventional aqueous outflow in vivo. Methods: This single-center randomized clinical trial included healthy adult volunteers who received GLAALPHA, a brimonidine tartrate–brinzolamide fixed-dose combination (Ailamide), or brimonidine tartrate monotherapy (Aiphagan) in a crossover sequence. The aqueous column width in the episcleral veins was assessed at baseline and at 2 h (primary outcome) and 8 h using hemoglobin video imaging. Results: Among 24 participants, analyses included 23 GLAALPHA-treated eyes, 21 Ailamide-treated eyes, and 22 Aiphagan-treated eyes. Two hours after instillation, the aqueous column width significantly increased from baseline only in the GLAALPHA group (p = 0.002). The percent increase in the aqueous column width at 2 h was significantly greater with GLAALPHA than with Ailamide (p = 0.039) and not significantly different between GLAALPHA and Aiphagan (p = 0.114). At 8 h, the aqueous column width did not differ from the baseline in any groups. Conclusions: In healthy adult eyes, GLAALPHA significantly increased the aqueous column width in the episcleral veins 2 h after instillation, indicating enhanced conventional aqueous outflow. These findings provide evidence that GLAALPHA promotes trabecular outflow beyond the effects of brimonidine tartrate-containing comparators and offer mechanistic insights into its action. Full article

Background/Objectives: Fragility fractures cause disability, independence loss, and death in older adults with osteoporosis. While exercise rehabilitation is recommended, its effectiveness and consistency in improving function, quality of life, and outcomes after a fracture remain uncertain. This study aimed to systematically assess the impact of structured exercise programs on physical function, quality of life, falls, morbidity, and mortality among adults recovering from fragility fractures. Methods: This review and meta-analysis followed PRISMA guidelines, with protocol registration in PROSPERO (CRD42024503933). MEDLINE, PubMed, EMBASE, Cochrane Library, CINAHL, and Web of Science were searched. Randomized controlled trials and quasi-experimental studies including adults (mean age ≥ 50 years) with fragility fractures were eligible if they evaluated structured exercise programs lasting ≥8 weeks compared with usual care or minimal intervention, and reported physical function, quality of life, falls, mortality, or morbidity outcomes. Risk of bias was assessed following the Cochrane Handbook for Systematic Reviews of Interventions guidelines. Primary outcomes comprised physical function, strength, balance, and health-related quality of life. Random-effects meta-analysis was applied where data were comparable. Results: Twenty-six studies with 4142 participants were included (n = 4142; ~80.4% women; mean age ~74.83 years). Interventions were mainly multicomponent (strength, balance, mobility, and functional training). Exercise improved physical function outcomes. At 12 months, pain measured by QUALEFFO-41 decreased (MD −11.61; 95% CI −22.99 to −0.23). Effects on strength, balance, and cognitive outcomes were inconsistent. Conclusions: Exercise-based rehabilitation after fragility fractures may improve physical function and reduce pain, but pooled effects are inconsistent and often highly heterogeneous. Evidence for effects on falls and mortality is sparse and does not support firm conclusions. Physical function measures may be the most practical primary endpoint for evaluating these interventions, interpreted cautiously. Funding: PIGE-0040-2020. Full article

Listeria monocytogenes is considered a highly persistent risk for public health in food production facilities. Food business operators manufacturing ready-to-eat foods (RTE) are required to sample processing areas for L. monocytogenes as part of their environmental monitoring plans. The aim of the study was to identify suitable sampling devices, demonstrating the crucial role of the sampling technique in the method performance for L. monocytogenes monitoring. Detection of Listeria spp. and L. monocytogenes from surfaces in an RTE food production facility was evaluated by using two different sampling methods (swabs and sponges). When using swabs, 46 sampling points were negative for both targets. However, when sampling same points with sponges, 30% samples (14 out of 46) were positive for Listeria spp. with 8 samples (17%) positive for L. monocytogenes. During subsequent in vitro experiments, L. monocytogenes strains spiked onto three different surfaces (stainless steel, Teflon and epoxy) showed recoveries of between 76% and 93% when using sponges, while in swabs, recoveries where always below 50%. All L. monocytogenes strains isolated belonged to the major clonal complexes (CC) circulating in Europe in food industry (e.g., CC121 and CC9) and none of them are considered among the hypervirulent strains. Genomic analysis, including new tools for source tracking (Gene Up Typer, bioMérieux) showed differences between strains isolated from different risk hygienic zones. Full article