Search Results (3,072)

Chrysanthemum (Chrysanthemum morifolium Ramat.), a typical short-day plant (SDP), relies on photoperiod and light quality signals to regulate flowering and growth. Red light interruptions inhibit its flowering, whereas supplemental blue light can counteract this inhibitory effect. To investigate how “high-blue/low-red” mixed light (RBL) regulates chrysanthemum flowering and growth, we treated ‘Gaya Glory’ plants with 4 h of supplemental or night-interruptional RBL (S-RBL4 or NI-RBL4, 0 or 30 ± 3 μmol m⁻² s⁻¹ PPFD) under 10 h short-day and 13 h long-day conditions (SD10 and LD13; white light, WL; 300 ± 5 μmol m⁻² s⁻¹ PPFD), recorded as SD10, SD10 + S-RBL4, SD10 + NI-RBL4, LD13, LD13 + S-RBL4, and LD13 + NI-RBL4, respectively. Under SD10 conditions, S-RBL4 promoted flowering and enhanced nutritional quality, whereas NI-RBL4 suppressed flowering. Under LD13 conditions, both treatments alleviated flowering inhibition, with S-RBL4 exhibiting a more pronounced inductive effect. Chrysanthemums displayed superior vegetative growth and physiological metabolism under LD13 compared to SD10, as evidenced by higher photosynthetic efficiency, greater carbohydrate accumulation, and more robust stem development. Furthermore, S-RBL4 exerted a stronger regulatory influence than NI-RBL4 on photosynthetic traits, the activities of sugar metabolism-related enzymes, and gene expression. The photoperiodic flowering of chrysanthemum was coordinately regulated by the photoreceptor-mediated and sugar-induced pathways: CmCRY1 modulated the expression of florigenic genes (CmFTLs) and anti-florigenic gene (CmAFT) to transmit light signals, while S-RBL4 activated sucrose-responsive flowering genes CmFTL1/2 through enhanced photosynthesis and carbohydrate accumulation, thereby jointly regulating floral initiation. The anti-florigenic gene CmTFL1 exhibited dual functionality—its high expression inhibited flowering and promoted lateral branch and leaf growth, but only under sufficient sugar availability, indicating that carbohydrate status modulates its functional activity. Full article

This study proposes a Personalized Deep Learning-Based Sleep Recommendation System Using Lifelog Data (PDSRS-LD). Traditional sleep research primarily relies on bio signals such as EEG and ECG recorded during sleep but often fails to sufficiently reflect the influence of daily activities on sleep quality. To address this limitation, we collect lifelog data such as stress levels, fatigue, and sleep satisfaction via wearable devices and use them to construct individual user profiles. Subsequently, real sleep data obtained from an AI-powered motion bed are incorporated for secondary training to enhance recommendation performance. PDSRS-LD considers comprehensive user data, including gender, age, and physical activity, to analyze the relationships among sleep quality, stress, and fatigue. Based on this analysis, the system provides personalized sleep improvement strategies. Experimental results demonstrate that the proposed system outperforms existing models in terms of F1 score and Average Precision (mAP). These results suggest that PDSRS-LD is effective for real-time, user-centric sleep management and holds significant potential for integration into future smart healthcare systems. Full article

This study investigated the effects of elevated temperature on the phenology and morphology of the early-maturing peach cultivar ‘Mihong’. The experiment was conducted from 2019 to 2024 in a temperature-gradient chamber at the National Institute of Horticultural and Herbal Science, Wanju, Korea, with four warming treatments (+2.2 °C to +5.0 °C above ambient). Higher temperatures delayed the onset of endodormancy and markedly shortened the period from endodormancy release to full bloom. Elevated temperatures also increased the LD ratio, with the proportion of fruits exceeding an LD ratio of 1.0 rising significantly with temperature. The LD ratio showed strong correlations with November mean temperature (MT11) and March maximum temperature (HT3) (r = 0.81) and was also associated with the average temperature (Temp3, r = 0.51) and duration (P3, r = −0.54) of the endodormancy release to full bloom phase. Stepwise and PLS regression identified temperatures in May, November, and March as key predictors of the LD ratio, while PCA revealed that temperature variables (Temp3, Temp5) and stage durations (P3, P4) were major contributors. These results confirm that climate warming alters the phenology and morphology of ‘Mihong’, reducing fruit quality and marketability, while providing a basis for predictive modeling and highlighting the importance of adaptive strategies such as shading or growth regulator application. Full article

Most botulinum toxin A (BoNT-A) products for esthetic use require reconstitution before administration. Ready-to-use relabotulinumtoxinA is a liquid manufactured using Precipitation-free Extraction and Activity-preserving, Refined Liquid (PEARL™) Technology from a proprietary C. botulinum type A1 strain. We examined the in vitro characteristics of relabotulinumtoxinA. The specific BoNT-A1 potency remained consistent throughout drug substance manufacturing (1.9 × 10⁸–2.2 × 10⁸ LD₅₀ mouse potency units/mg of BoNT-A1, four fractions sampled). Using glabellar line (GL) on-label doses, relabotulinumtoxinA liquid product was compared with powder onabotulinumtoxinA using the following: BoNT-A1 amount based on ELISA; specific enzyme activity based on SNAP-25 cleavage by a fluorescence resonance energy transfer-based assay (BoTest^®); biological activity (binding, internalization, and SNAP-25 cleavage over time) using a cell-based assay. RelabotulinumtoxinA contained more BoNT-A1 per on-label GL dose (0.27 ng) than onabotulinumtoxinA (0.18 ng), had higher enzyme activity (53 vs. 29 BoTest^® units) per GL dose, and had higher specific activity per pg BoNT-A, with onabotulinumtoxinA displaying 81% of the specific activity of relabotulinumtoxinA. In vitro, relabotulinumtoxinA demonstrated higher biological activity and earlier onset of SNAP-25-cleavage than onabotulinumtoxinA. PEARL^TM Technology thus produces high-quality BoNT-A1 with high specific enzyme and biological activities, which may explain the clinical performance of relabotulinumtoxinA in Phase 3 clinical trials examining treatment of GLs and/or LCLs. Full article

Simulation-based studies can support breeders’ decisions inexpensively, since there is no need to perform a new procedure. The objective was to assess the efficiency of recurrent genomic selection in panmictic population under additive–dominance and additive–dominance with epistasis models. We assumed two broiler chicken populations with contrasting linkage disequilibrium (LD) levels, 38,500 SNPs, and 1000 genes controlling feed conversion ratio. We applied recurrent genomic selection over seven cycles. The genomic selection efficacy, expressed as realized total genetic gain, was proportional to the LD level and genotypic variance. Genomic selection required model updating to achieve a higher efficacy. The training set size required by genomic selection can be as low as 10%/generation. Under this low-cost scenario, the genomic selection efficacy was slightly lower than the maximum efficacy. There is no difference between genetic evaluation methods regarding the decrease in the genotypic variance due to selection. In general, additive value prediction accuracies and realized genetic gains were highly correlated. The accumulated inbreeding level was not high due to avoidance of sib cross. The genomic inbreeding coefficient over generations was close to zero. Except for dominant epistasis, the efficacy of genomic selection was 4.1 to 46.2% lower than the efficacy under no epistasis. Full article

Background/Objectives: The glymphatic system is a recently characterized glial-dependent waste clearance pathway in the brain, which makes use of perivascular spaces for cerebrospinal fluid exchange. Diffusion tensor imaging analysis along the perivascular space (DTI-ALPS) offers a non-invasive method for estimating perivascular flow, but its biological specificity and susceptibility to methodological variation, particularly head position during MRI acquisition, remain as threats to the validity of this technique. This study aimed to assess the prevalence of current DTI-ALPS practices, evaluate the impact of head orientation on ALPS index calculation, and propose a novel computational approach to improve measurement validity. Methods: We briefly reviewed DTI-ALPS literature to determine the use of head-orientation correction strategies. We then analyzed diffusion MRI data from 172 participants in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) to quantify the influence of head orientation on ALPS indices computed using the conventional Unrotated-ALPS, a vecrec-corrected ALPS, and the new LD-ALPS method proposed within. Results: A majority of studies employed Unrotated-ALPS, which does not correct for head orientation. In our sample, Unrotated-ALPS values were significantly associated with absolute head pitch (r₁₆₉ = −0.513, p < 0.001), indicating systematic bias. This relationship was eliminated using either vecreg or LD-ALPS. Additionally, LD-ALPS showed more sensitivity to cognitive status as measured by Mini-Mental State Examination scores. Conclusions: Correcting for head orientation is essential in DTI-ALPS studies. The LD-ALPS method, while computationally more demanding, improves the reliability and sensitivity of perivascular fluid estimates, supporting its use in future research on aging and neurodegeneration. Full article

Citrobacter braakii is an emerging opportunistic pathogen of escalating clinical significance in animal hosts, though its pathogenic mechanisms remain poorly characterized. This study isolated a C. braakii strain (SCGY-1L) from diseased Siniperca chuatsi and confirmed its identity through integrated morphological, physiological, and molecular analyses. Comprehensive genomic sequencing revealed a 5.75 Mb genome comprising one circular chromosome and two plasmids. A Circos plot was constructed to visualize the genomic architecture of strain SCGY-1L, revealing 5482 protein-coding genes, 25 tRNA genes, and 86 rRNA genes. Additionally, 738 virulence-associated genes and 366 antibiotic resistance determinants were annotated, elucidating multidrug-resistant phenotypes including insensitivity to erythromycin and penicillin. Pathogenicity assessment established an LD₅₀ of 1.28 × 10⁶ CFU/mL in infected hosts, with histopathological analysis showing significant hemorrhage and necrosis in target organs (liver, spleen, kidney). Host transcriptome profiling generated 41.21 Gb of high-quality clean data, identifying 2201 differentially expressed genes post-infection (1568 up-regulated; 633 down-regulated). These were significantly enriched in phagocytosis, cytokine-mediated signaling, and inflammatory regulation pathways. These molecular insights establish C. braakii’s mechanistic framework for pathogenesis and host adaptation, providing critical targets for diagnostics and therapeutics against emerging Citrobacter infections. Full article

This study examines the genetic diversity and population structure of silver carp (Hypophthalmichthys molitrix), an ecologically and economically important freshwater species. Samples were collected from 17 sites along the Yangtze River, including LCH, LCS, LJHK, and LXZX, as well as one population from the United States (SV). Restriction-site associated DNA sequencing (RAD-seq) generated 759,453 high-quality single-nucleotide polymorphisms (SNPs) for population genomic analyses, including genetic differentiation (F_ST), population structure, and linkage disequilibrium (LD) decay. Genetic variation was primarily found within populations (78.05%), with 21.94% among populations. Most sites exhibited low genetic differentiation (F_ST < 0.05), suggesting high admixture along the river, although a few sites displayed elevated values (F_ST > 0.15). Rapid LD decay in LCH, LCS, and LJZ indicated frequent recombination and moderate to large effective population sizes. These patterns reflect the influence of geographic and ecological factors on population structure. Conservation strategies should maintain genetic connectivity while protecting distinct genetic resources. Populations with high differentiation, such as LXZX and LWZ, warrant targeted management to preserve unique genetic diversity. Full article

Nanodiamonds (NDs) are an innovative material in biomedical applications based on their excellent biocompatibility, nanoscale dimensions, and high surface area. In this study, we evaluated the potential of ND-in-oil emulsion to induce potent antibody responses in animals immunized with cobra venom. NDs demonstrated the capacity to bind complex venom proteins as stable conjugates, well dispersed in aqueous solution. Immunization of mice with cobra venom incorporated with ND-in-oil emulsion adjuvant (ND/venom) elicited strong venom-specific antibody responses with titers comparable to those induced by venom formulation with conventional Freund’s adjuvants (FA/venom). IgG subclass analysis revealed that ND- and FA-based formulations induced a Th2-biased immune response in mice. Moreover, antibodies elicited by ND/venom or FA/venom immunization specifically recognized the epitopes of the lethal component of short-chain neurotoxin and conferred full protection against lethal cobra venom challenge (3LD₅₀). Further, ND/venom hyperimmunization was capable of inducing high levels of neutralizing antibodies in larger animals, rabbits, highlighting the potential for antivenom manufacturing. Notably, there were no obvious lesions at the injection sites of animals that received ND/venom, in contrast to those that received FA/venom. These findings indicated NDs as an effective and safe additive in venom formulation for antivenom production. Full article

Increased hepatic triacylglycerol (TG) storage in lipid droplets (LDs) is a hallmark of metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH). Human carboxylesterase 1 (CES1) regulates TG storage and secretion in hepatocytes, but the mechanism remains to be elucidated. We performed studies in rat hepatoma McArdle RH7777 cells stably transfected with CES1 cDNA and in Ces1d-deficient mice using a variety of biochemical, pharmacological and cell biology approaches including the assessment of gene expression, confocal immunofluorescence microscopy, lipid synthesis measurements and quantitative mass spectrometry. CES1-expressing cells accrued more TG compared to cells lacking CES1 when incubated with oleic acid. CES1 increased the expression of Srebf1c, Nr1h3 and Nr1h2 encoding transcription factors (SREBP1c and LXRα and LXRβ, respectively) that regulate the expression of lipogenic genes. Additionally, CES1 increased the expression of Acsl1 encoding an enzyme catalyzing fatty acid activation and the expression of Dgat1 and Dgat2 encoding enzymes catalyzing TG synthesis. Treatment of CES1-expressing cells with PPARγ antagonist (GW9662), LXR antagonist (GSK2033) or CYP27A1 inhibitor Felodipine prevented CES1-mediated fatty acid esterification into TG. Ces1d-deficient mice fed high-fat diet (HFD) presented with decreased expression of Nr1h3, Nr1h2, Srebf1c and reduced hepatic TG content. Felodipine and GSK2033 treatment eliminated the differential effects on TG concentration between wild-type and Ces1d-deficient hepatocytes. The results suggest that CES1/Ces1d activates PPARγ, LXR and SREBP1c pathways, thereby increasing TG synthesis and LD storage by augmenting fatty acid esterification. Full article

Dynamic compression (DC) bioreactors are widely used to mimic joint loading and study how human mesenchymal stem cells (hMSCs) respond to mechanical cues. However, it remains unclear whether DC alone is sufficient to induce chondrogenesis or how such cues interact during construct maturation. In this study, hMSCs were encapsulated in fibrin hydrogels at different cell densities and subjected to DC without, during, or after TGF-β3-mediated chondrogenic induction. DC alone modestly increased SOX9 expression but failed to upregulate key cartilage matrix genes such as ACAN and COL2A1, indicating that mechanical stimulation alone is insufficient to initiate chondrogenesis. When mechanical stimulation was coupled with TGF-β3, a more mature chondrogenic phenotype was observed for high cell seeding densities (HD). To simulate a post-implantation scenario, we applied DC following growth factor withdrawal and observed marked downregulation of SOX9, ACAN, and COL2A1 in low-density (LD) constructs. This reduction was not observed in HD constructs, which maintained a more stable chondrogenic phenotype under loading. These findings show that construct maturation critically influences mechanoresponsiveness and suggest that immature grafts may not tolerate mechanical stimulation. DC bioreactors may therefore serve not only to support cartilage engineering but also to predict in vivo graft performance. Full article

Transgenic insect-resistant maize can effectively control insect pests, which is of great significance to improve maize yield and quality. Transgenic maize LD05 is an insect-resistant and herbicide-tolerant maize independently developed by Shandong Academy of Agricultural Sciences and highly resistant to major lepidopteran pests. In order to study the pest resistance of transgenic maize LD05 in different ecological areas of China, this study conducted a laboratory bioassay, and artificial inoculation test and natural pest investigation in field were carried out in one pilot of each of five maize ecological zones in China. The results of laboratory bioassay showed that transgenic maize LD05 had high resistance to Ostrinia furnacalis (Guenée), Mythimna separata (Walker), Helicoverpa armigera (Hübner) and Spodoptera frugiperda (J. E. Smith), the main lepidopteran pests threatening maize production in China. The results of artificial inoculation test and natural pest investigation in field showed that transgenic maize LD05 had high resistance to major lepidopteran pests in different ecological areas of China, which was consistent with the pest resistance management strategy, and can provide important theoretical basis and technical support for the industrialization of transgenic maize LD05 in the future. Full article

As modern Advanced Driver Assistance Systems become increasingly prevalent in the automotive industry, Lane Detection (LD) solutions play a key role in enabling vehicles to drive autonomously or provide assistance to the driver. Many modern LD algorithms are based on neural networks, which estimate the locations of lane markings as segmentation masks in the input image. In this paper, we propose a novel algorithm, named SMPPALD (Segmentation Mask Post-Processing Algorithm for improved Lane Detection), designed to perform a set of post-processing operations on these segmentation masks to produce a list of points that define the lane markings. These operations follow geometric and contextual rules, taking into account the LD problem and improving detection accuracy. The algorithm was tested using the well-known and widely used Spatial Convolutional Neural Network (SCNN) on three different datasets (CULane, TuSimple, and LLAMAS). SMPPALD achieved a significant improvement in terms of F1 measure compared to SCNN on the TuSimple and LLAMAS datasets, while for the CULane dataset, it outperformed SCNN in most categories. Full article

This study aimed to characterize and analyse the kinematic parameters and muscle activity of swimmers in open water swimming (OWS). Nine male swimmers (age: 25.4 ± 11.9 years; body mass: 75.9 ± 9.0 kg; height: 180.7 ± 6.7 cm; and arm span: 185.6 ± 10.3 cm) were evaluated in an open environment (lake), performing 5 m × 1000 m at maximum intensity, with a rest of 30 s every 1000 m. For kinematical analyses, the stroke rate (SR), swimming velocity (v), stroke length (SL), and stroke index (SI) were calculated. Surface EMG data were recorded in seven muscles—upper trapezius (UP); latissimus dorsi (LD); pectoralis major (PM); posterior deltoid (PD); anterior deltoid (AD); triceps brachii (TB); and biceps brachii (BB)—for the underwater and recovery phases of the stroke. SL (F = 3.41, p = 0.06, η² = 0.30) and SI (F = 3.29, p = 0.08, η² = 0.29) changed along the covered distances, and SR (F = 1.54, p = 0.24, η² = 0.16) increased, especially in the last 1000 m (32.5 ± 3.0 cycles-min⁻¹). AD was highly activated in recovery, showing statistical differences from the beginning (p ≤ 0.01) to the end of the race (p = 0.03). The TB muscle was mostly recruited in the underwater phase, from the start (p ≤ 0.01) to the finish (p = 0.03), showing a significant difference in each lap, with a large effect. LD showed significant differences in muscle activation, from 1000 m (p ≤ 0.01) with a huge effect, to 5000 m (p ≤ 0.01), with a large effect. These results suggested that the UT and AD muscles had higher activity in recovery than the underwater phase, and TB and LD were higher in the underwater phase. Full article

Analysis of selection signatures in the genomes of farm animals enables the detection of genomic regions affected by selection and contributes to the identification of genes underlying adaptive and productive traits. This research aimed to identify loci under selection pressure and to detect candidate genes in Dzhalgin Merino sheep by performing a comparative genomic analysis with the related Australian Merino and Rambouillet breeds. A total of 293 animals were included in the analysis, comprising Dzhalgin Merino (n = 53), Australian Merino (n = 50), Australian Industry Merino (n = 88), and Rambouillet (n = 102). Whole-genome SNP genotyping data for Dzhalgin Merino were generated within this study, while data for Australian Merino, Australian Industry Merino, and Rambouillet were obtained from the SheepHapMap project. For the purposes of analysis, Australian Merino and Australian Industry Merino were combined into a single group (n = 138). To enhance the reliability of the results, three independent methods were employed to detect selection signatures: the fixation index (FST), analysis of linkage disequilibrium variation (varLD), and the cross-population number of segregating sites by length (xp-nSL). The study showed that Dzhalgin Merino have unique genetic signatures potentially associated with adaptation and productivity, which opens up new opportunities for their selection. The identified genes can become the basis for developing new breeding programs aimed at improving both the productive qualities and the adaptive abilities of the breed. Further research should be aimed at a detailed investigation of gene structure within loci under selection pressure and at clarifying the mechanisms by which these genes influence animal phenotypes. A total of 185 genes were identified within genomic regions exhibiting selection signatures. Among these, particular attention was given to EPHA6, MLLT3, ROBO1, KIAA0753, MED31, SLC13A5, and ELAVL4, which are involved in biological processes such as growth, development, and reproduction. The identified genes represent potential targets for breeding programs aimed at increasing productivity and adaptive capacity of the breed. Full article