Search Results (77,609)

A novel partial discharge (PD) source localization method was proposed based on the traditional time difference in arrival (TDOA) method. Specifically, the non-line-of-sight (NLOS) propagation phenomenon of the ultra-high-frequency (UHF) signal was considered, and the NLOS propagation error was approximately replaced by a constant, thereby limiting the effect of NLOS propagation. Moreover, the strategy of utilizing more than four sensors was adopted to reduce the possible effect of overcorrection on NLOS propagation. In this paper, the derivation and implementation process of the proposed method is introduced from the perspectives of mathematical model and geometrical model, and its localization results were compared with the traditional TDOA method through an experimental study. The results showed that the speed of error increase of the traditional method presented faster, and the increment of sensor number helped to improve the localization accuracy, but the reduction in localization error becomes insignificant when the sensors exceed six. Finally, the experimental verifications were conducted based on a 35 kV testing transformer with six sensor installations. The experiments found that the proposed localization method had a better calculated accuracy and stability; the obtained minimum calculated error was 10.88 cm, the calculated accuracy can be improved by 82.04% and 78.94%, respectively, with six sensors than four and five sensors arrangement. Full article

Cellular senescence can occur with similar phenotypes in normal cells, during aging, and in tumor cells, spontaneously or after cytostasis. The fall or increase in proliferative activity are key aspects of the respective conditions, in which the levels of reactive oxygen species can vary, affecting the cellular redox homeostasis. This work aimed to study the relationships between senescence and transformation by comparing cells with different proliferative activities and phenotypes attributable to transformation (NIHs cultures) or senescence (NIHv cultures), before and after incubation with hydrogen peroxide. Both cultures were derived from the NIH/3T3 cell line, which was used here as a reference (NIHb), after the serum starvation. Our experimental model can be representative of the heterogeneity of cell subpopulations, with different degrees of transformation and senescence, found in some tumors. The characterization of the functional properties of NIHb, NIHs, and NIHv cells was performed by a morphocytometric analysis of the cell cycle progression, mitochondrial and lysosomal content/activity, and superoxide anion production. The efficiency of the lysosomal compartment was also assessed by estimating the autophagic activity and measuring lipofuscin autofluorescence. Comparisons of nuclear and cytoplasmic parameters before and after the incubation with hydrogen peroxide revealed differences in the expression and modulation of cellular senescence patterns. The treatment effects were very limited in the NIHb culture; the senescence condition was essentially maintained in the NIHv cells, while the most relevant changes were found in the NIHs cells. In the latter, the acquisition of the senescent phenotype, also demonstrated by the positivity of SA-β-galactosidase, was correlated with a decrease in proliferative activity and a change in the content/activity of the mitochondria and lysosomes, which showed similarities with the basal senescence conditions of NIHv cells. In NIHs cells, increased autophagy events and lipofuscin accumulation also indicate the establishment of cytoplasmic dynamics typical of senescence. The variable responses to hydrogen peroxide, besides depending on the different basal cytokinetic activity of the cultures examined, appeared to be related to the specific cell redox state resulting from the balance between endogenous ROS and those produced after treatment. Especially in NIHs cells, the slowing down of the cell cycle was linked to dynamic interconnections between the mitochondrial and lysosomal compartments. This would indicate that transformed cells, such as NIHs, may express morpho-functional aspects and markers typical of cellular senescence, as a consequence of the modulation of their redox state. Full article

The occurrence of the welding heat-affected zone in Q420C steel may lead to a reduction in the toughness of the welded joint and disruption of high strength-toughness combination of Q420C. This study investigates the microstructure and mechanical properties of Q420C steel welded joints under three heat in-puts of 25 kJ/cm, 100 kJ/cm, 200 kJ/cm, and 300 kJ/cm, with high-strength matching adopted for the welded joints, Charpy impact tests at 0 °C, −20 °C, and −40 °C were conducted on the weld metal, fusion line(FL), and heat-affected zone (HAZ). The weld metal maintains high impact toughness across all tested temperatures. However, increasing the heat input leads to coarsening of the microstructure in the overheated zone of the HAZ, accompanied by the formation of ferrite. At a heat input of 300 kJ/cm, significant amounts of coarse intergranular ferrite and intragranular blocky ferrite develop in the overheated zone. These microstructural changes result in a marked reduction in the impact toughness of both the fusion zone and HAZ, and the fracture mode shifts from ductile to cleavage fracture. To ensure adequate impact toughness of Q420C welded joints, the welding heat input should be kept below 200 kJ/cm. Full article

This paper proposes a new proximal iteratively reweighted nuclear norm method for a class of nonconvex and nonsmooth optimization problems. The primary contribution of this work is the incorporation of line search technique based on dimensionality reduction and extrapolation. This strategy overcomes parameter constraints by enabling adaptive dynamic adjustment of the extrapolation/proximal parameters (α_k, β_k, μ_k). Under the Kurdyka–Łojasiewicz framework for nonconvex and nonsmooth optimization, we prove the global convergence and linear convergence rate of the proposed algorithm. Additionally, through numerical experiments using synthetic and real data in matrix completion problems, we validate the superior performance of the proposed method over well-known methods. Full article

Although opioids are effective in treating pain, they cause serious side effects. The use of regional anesthesia, although effective in the perioperative period, may not be suitable if mobility and lack of numbness is desired. Hence, there is a clear need for novel pain therapies. Low-voltage activated (T-type) calcium channels (Ca_V3.2 isoform) could be a promising therapeutic target for the development of novel pain therapies. Indeed, our published findings suggest that novel neuroactive steroid (NAS) analogs that modulate the activity of Ca_V3.2 channels have unique anti-nociceptive properties. However, the concern with current NASs appears to be their hypnotic/sedative properties, thus potentially hindering the future development of NASs for novel pain therapies. Hence, we developed a new line of NASs that are effective blockers of neuronal Ca_V3.2 channels in pain pathways while having more favorable pharmacodynamic properties, i.e., lack of sedative/hypnotic side effects. We present two promising novel analogs of NASs—B372 ((3β,5α,17β)-3-Hydroxyandrostan-17-carbonitrile) and YX23 ((3β,5α,17β)-3-Methoxyestran-17-ol). Using an in vitro approach, we show that B372 and YX23 are effective in blocking Ca_V3.2 channels. Using an in vivo approach, we show that they are effective anti-nociceptives in wild-type but not Ca_V3.2 knock-out mice. Importantly, we show that they lack sedative/hypnotic effects. Full article

This study delves into the response characteristics of core grounding current signals in power transformers across different operating conditions, aiming to enhance the accuracy of transformer condition assessment. Existing detection technologies often rely on single-parameter methods, which fall short in providing a comprehensive evaluation of transformer conditions. To address this limitation, this research develops a wideband circuit model based on multi-conductor transmission line theory and backed by experimental validation. The model systematically investigates the response mechanisms of core grounding current to various electrical stresses, including impulse voltages, power-frequency harmonics, and partial discharges. The findings reveal distinct response characteristics of core grounding current under different stresses. Under impulse voltage excitation, the core current exhibits high-frequency oscillatory decay with characteristics linked to voltage waveform parameters. In harmonic conditions, the current spectrum shows linear correspondence with excitation voltages, with no resonance below 1 kHz. Partial discharges induce high-frequency oscillations in the grounding current due to multi-resonant networks formed by distributed winding-core parameters. This study establishes a new theoretical framework for transformer condition assessment based on core grounding current analysis, offering critical insights for optimizing detection technologies and overcoming the limitations of traditional methods. Full article

This report presents the case of a seven-year-old West Highland White Terrier diagnosed with relapsed and refractory multiple myeloma (MM), managed using multiple treatment approaches, including conventional chemotherapy (melphalan, vincristine, doxorubicin, and dexamethasone), radiation therapy (RT), and novel agents such as the selective inhibitor of nuclear export (verdinexor), proteasome inhibitors (bortezomib, carfilzomib, and ixazomib), and tyrosine kinase inhibitors (TKIs; toceranib and sorafenib). Treatment response was monitored using serum globulin concentration and imaging studies. Verdinexor achieved the longest period of stable remission with minimal toxicity post-RT. Bortezomib + dexamethasone was effective in controlling hyperglobulinemia at doses ≥ 1.45 mg/m², although cumulative hematologic and gastrointestinal toxicity limited its prolonged use. Second-line proteasome inhibitors and TKIs demonstrated limited efficacy. Despite initial therapeutic response, the patient’s condition deteriorated due to persistent hyperglobulinemia and hyperviscosity syndrome. The absence of advanced supportive options, including plasmapheresis, contributed to a fatal outcome. This case highlights the potential utility of novel therapies such as verdinexor and bortezomib in managing refractory canine MM. Timely intervention, individualized dosing, and supportive care are essential for optimizing treatment outcomes. Further research is required to define effective combinations and integrate advanced care options, including stem cell transplantation and targeted antibody therapies, in veterinary MM. Full article

Anthropogenic disturbances interact with wildfire, altering successional dynamics across North America’s boreal forest. Linear disturbances, including seismic lines used for oil and gas exploration, dissect forests, while wildfire is a fundamental agent of forest succession. However, little is known about early succession dynamics after both seismic line creation and wildfire, especially across transitions from uplands to peatlands. To address this, we characterized and compared regeneration and recruitment after individual and successive disturbances in peatland, transitional, and mesic upland forests across the oil sands region of Alberta, Canada. We used non-metric multidimensional scaling to compare composition and mixed-effects generalized linear models to compare densities of trees and tall shrubs 10 to 24 years after disturbance. Compositionally, regeneration was similar within forest types and between transitional and peatland forests, while patterns in recruitment were more influenced by past disturbances. Overall, we found evidence of dominant, additive, and interactive effects on early successional patterns within linear disturbances in boreal forests. In transitional and peatland forests, disturbances influenced tree and tall shrub regeneration and recruitment in complex ways. Early successional dynamics after disturbance influence forest structure and composition and are vital to understanding recovery in boreal forests, especially across boreal forest transitions. Full article

Downy mildew, caused by the oomycete Pseudoperonospora cubensis, is the most destructive foliar disease of cucumbers. While partially resistant slicer cultivars (with spined fruits) are commercially available, no resistant Beit Alpha cultivars (characterized by smooth, dark green fruit) have been developed to date. Here, we report the successful breeding of downy mildew-resistant Beit Alpha cucumber lines. Resistance was transferred from the wild Sikkim cucumber accessions PI 197088 and PI 330628 (characterized by round fruit, with heavily netted brown rind). The resistance and fruit phenotype were restored through backcrosses to elite commercial susceptible cultivars. Due to the recessive nature of the resistance genes and their distribution across multiple chromosomes, the breeding program required multiple backcrosses and stringent selections for both resistance and fruit type. Full article

Simultaneous Localization and Mapping (SLAM) is a critical technology for robot intelligence. Compared to cameras, Light Detection and Ranging (LiDAR) sensors achieve higher accuracy and stability in indoor environments. However, LiDAR can only capture the geometric structure of the environment, and LiDAR-based SLAM often fails in scenarios with insufficient geometric features or highly similar structures. Furthermore, low-cost mechanical LiDARs, constrained by sparse point cloud density, are particularly prone to odometry drift along the Z-axis, especially in environments such as tunnels or long corridors. To address the localization issues in such scenarios, we propose a forward-enhanced SLAM algorithm. Utilizing a 16-line LiDAR and a monocular camera, we construct a dense colored point cloud input and apply an efficient multi-modal feature extraction algorithm based on centroid distance to extract a set of feature points with significant geometric and color features. These points are then optimized in the back end based on constraints from points, lines, and planes. We compare our method with several classic SLAM algorithms in terms of feature extraction, localization, and elevation constraint. Experimental results demonstrate that our method achieves high-precision real-time operation and exhibits excellent adaptability to indoor environments with similar structures. Full article

Achieving net zero relies on siting large-scale solar and wind where they are cheapest and most socially acceptable. We present a transferable, evidence-based siting framework and apply it to Australia. The landscape is divided into millions of 250 m pixels, each assigned an indicative cost based on resource quality, distance-weighted connection costs, and land use exclusions. Two bounding generation mix scenarios (high solar vs. high wind) stack the cheapest pixels until a fully electrified demand of 20 MWh per capita per year is met. Results are aggregated to all 547 Local Government Areas (LGAs) and 150 federal electorates and expressed as capital inflow, construction job-years, long-term jobs, and land-lease income. We find Class A solar (<50 AUD/MWh) is abundant nationwide except in Tasmania, while high-quality wind is concentrated in Victoria, Tasmania, and coastal Western Australia. Just 15% of LGAs, mainly within 100 km of the existing 275–500 kV transmission backbone, can host over half of least-cost capacity. A single top-ranked LGA such as Toowoomba (Queensland) could attract around AUD 33 billion in investment and sustain over 50,000 construction job-years. Mapping ten candidate high-voltage transmission corridors shows how new lines shift opportunities to under-served councils. The results bridge the gap between state-level renewable energy zones and fine-scale site suitability maps, with policy recommendations proposed. Because the workflow relies mainly on globally available datasets, it can be replicated in other countries to raise public awareness, align policy with community support, and accelerate clean-energy buildouts while maximising regional benefit. Full article

The immune response to SARS-CoV-2 infection involves significant alterations in the phenotype and function of natural killer (NK) cells. This study aimed to investigate the dynamic changes in NK cell subsets during COVID-19 by analyzing their activation and inhibitory markers [CD3, CD14, CD16, CD19, CD25, CD45, CD56, CD57, CD69, CD159a (NKG2A), CD159c (NKG2C), CD314 (NKG2D), CD335 (NKp46)], cytotoxic potential (perforin, interferon-gamma, granzyme B), and direct cytotoxicity against a newly genetically modified K562 cell line. Peripheral blood samples were collected from COVID-19 patients on days 3–5 and day 30 post-symptom onset and were compared to healthy controls. 16-color flow cytometry analysis revealed distinct shifts in NK cell subpopulations, characterized by increased expression of the inhibitory receptor NKG2A and the activating receptors NKG2D and NKG2C, particularly in the CD56⁺CD16⁻ subset. Elevated IFN-γ production on day 30 suggested a recovery-phase immune response, while the persistent upregulation of NKG2A indicated an ongoing regulatory mechanism. The CD16⁺CD56⁻ subpopulation exhibited increased expression of the markers CD69 and CD25 over time; however, its cytotoxic potential, assessed through granzyme B levels and direct cytotoxicity assays, remained lower than that of healthy controls. Significant correlations were observed between CD57 and CD69 expression, as well as NKp46 and IFN-γ production, highlighting a coordinated balance between activation and regulatory mechanisms. These findings suggest that NK cells undergo functional adaptation during COVID-19, displaying signs of partial exhaustion while retaining antiviral potential. Understanding the interplay between NK cell activation and suppression may provide valuable insights into immune dysregulation in COVID-19 and inform potential therapeutic interventions. Full article

Unmanned Aerial Vehicles (UAVs) face collision risks due to Beyond Visual Line of Sight operations. Therefore, UAV Traffic Management (UTM) systems are used to manage and monitor UAV flight paths. However, centralized UTM systems are susceptible to various security attacks and are inefficient in managing flight data from different service providers. It further fails to provide low-latency communication required for UAV real-time operations. Thus, this paper proposes to integrate Drone-to-Drone (D2D) communication protocol into a secure public blockchain-based UTM system to enable direct communication between UAVs for efficient collision avoidance. The D2D protocol is designed using SHA256 hash function and bitwise XOR operations. A proof of concept has been built to verify that the UTM system is secure by enabling authorized service providers to view sensitive flight data only using legitimate secret keys. The security of the protocol has been analyzed and has been proven to be secure from key disclosure, adversary-in-the-middle, replay, and tracking attacks. Its performance has been evaluated and is proven to outperform existing studies by having the lowest computation cost of 0.01 ms and storage costs of 544–800 bits. Full article

This study compares the Stackelberg game model and the entropy weight method for allocating intercity water rights in Heilongjiang Province (2014–2021). The entropy method objectively determines indicator weights, while the Stackelberg framework simulates leader–follower interactions between the water authority and users to balance efficiency and satisfaction. Under the same total water rights cap, the Stackelberg scheme achieves a comprehensive benefit of CNY 14,966 billion, 4% higher than the entropy method (CNY 14,436 billion). The results and comprehensive benefits of the two schemes are close to each other in the cities of Qiqihaer, Daqing, Hegang, etc., but the allocation method of the game theory is more in line with the practical needs and can meet the water demand of each region, and the entropy right method is more useful for the cities of Jiamusi, Jixi, and Heihe, while for other cities the water rights allocation appeared to be unreasonable. While the entropy approach is transparent and data-driven, it lacks dynamic feedback and may under- or over-allocate in rapidly changing contexts. The Stackelberg model adapts to varying demands, better aligning allocations with actual needs. We discuss parameter justification, sensitivity, governance assumptions, and potential extensions, including hybrid modeling, climate change integration, stakeholder participation, and real-time monitoring. The findings provide methodological insights for adaptive and equitable water allocation in regions with strong regulatory capacity. Full article

Background/Objectives: Esophageal and esophagogastric junction adenocarcinoma (EADC-EGJA), which mainly develops from Barrett’s esophagus (BE), low-grade dysplasia (LGD), and high-grade dysplasia (HGD), has a poor prognosis and several unmet clinical needs, among which is the detection of minimal residual disease (MRD) after endoscopic/surgical resection. Long interspersed nuclear element-1 (LINE-1), a surrogate marker of global methylation, is considered an emerging biomarker for MRD monitoring. The aim of this study was to determine, by LINE-1 methylation analysis, at which carcinogenesis step global methylation is affected and whether this biomarker could be followed in longitudinal to monitor the disease behavior post-surgery. Methods: Cell-free DNA of 90 patients with non-dysplastic Barrett’s esophagus (NDBE), HGD/early EADC-EGJA, or locally advanced/advanced EADC-EGJA were analyzed for LINE-1 methylation, by Methylation-Sensitive Restriction Enzyme droplet digital PCR (MSRE-ddPCR). Twenty-six patients were longitudinally studied by repetitive blood sampling. Results: Global hypomethylation increased during carcinogenesis, with significant difference between locally advanced/advanced EADC-EGJA and NDBE patients (p = 0.028). Longitudinal cases confirmed the rareness of hypomethylation in NDBE cases. The majority of HGD/early EADC-EGJA and locally advanced/advanced EADC-EGJA patients showed methylation changes after resection according to clinical status. Conclusions: This study suggests that global hypomethylation occurs just prior to cancer invasiveness and that it is a promising biomarker to monitor MRD. Full article