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Search Results (502)

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12 pages, 781 KB  
Article
The Role of Meta-Emotional Intelligence in Behavioral Rule Knowledge
by Antonella Chifari, Antonella D’Amico, Alessandro Geraci, Luciano Seta and Giuseppe Chiazzese
J. Intell. 2025, 13(11), 136; https://doi.org/10.3390/jintelligence13110136 - 27 Oct 2025
Viewed by 258
Abstract
Emotional intelligence (EI) and its meta-cognitive counterpart, meta-emotional intelligence (MEI), have increasingly been recognized as key factors in helping students understand, regulate, and reflect on their emotional experiences. MEI expands upon EI by incorporating meta-cognitive beliefs and awareness about one’s own emotional functioning, [...] Read more.
Emotional intelligence (EI) and its meta-cognitive counterpart, meta-emotional intelligence (MEI), have increasingly been recognized as key factors in helping students understand, regulate, and reflect on their emotional experiences. MEI expands upon EI by incorporating meta-cognitive beliefs and awareness about one’s own emotional functioning, thereby influencing both emotional regulation and positive behavioral choices. This study examined the relationship between MEI and the knowledge of positive behavioral rules among 198 students aged 9 to 12. Participants completed the IE-ACCME-B, which assesses meta-emotional beliefs, emotional self-conceptualization, and emotional abilities, along with the PBIS-KGVE, a tool developed ad hoc to measure knowledge, generalization, and value-based understanding of school rules. Findings highlight that almost all considered variables are intercorrelated, with meta-emotional beliefs being the best predictor of the students’ knowledge, generalization, and value-based interpretation of behavioral rules. These results suggest the opportunity to establish interventions focused on meta-emotional beliefs to enhance behavioral rule knowledge and foster prosocial development within educational contexts. Full article
18 pages, 620 KB  
Article
Prejudice Formation in Childhood: How Parental Bonding Can Affect Social Dominance Orientation
by Serenella Tolomeo, Shannen Koh and Gianluca Esposito
Brain Sci. 2025, 15(11), 1147; https://doi.org/10.3390/brainsci15111147 - 25 Oct 2025
Viewed by 309
Abstract
Background: How individuals develop and form perspectives of those around them differs from person to person. Factors such as childhood parental bonding styles can affect how prejudice forms. Social dominance in adulthood may also be affected by childhood experiences through the bonding received. [...] Read more.
Background: How individuals develop and form perspectives of those around them differs from person to person. Factors such as childhood parental bonding styles can affect how prejudice forms. Social dominance in adulthood may also be affected by childhood experiences through the bonding received. Not many studies examine how an individual’s Social Dominance Orientationcan be influenced by parental bonding styles in childhood. Furthermore, few studies that investigated neural correlates are associated with these two variables. As such, this study aims to establish how parental bonding in childhood affects brain regions that are also implicated in adult SDO. Methods: Ninety-one participants were recruited and underwent magnetic resonance imaging (MRI). Social Dominance Orientation (SDO) and Parental Bonding Index (PBI) were collected. We used DARTEL package in SPM12 to conduct a whole-brain analysis. The ROI analyses were focused on amygdala grey matter volume (GMV). Results: This study identified a strong correlation between PBI and SDO. Interestingly, PBICare and PBIProtection scores significantly predicted SDO scores. SDO was positively associated with amygdala GMV, PBICare was negatively associated with amygdala GMV, and PBIProtection was positively associated with amygdala GMV. Conclusions: Our results show that PBI and SDO are highly correlated as well as their association with the amygdala and other key regions of the brain. Full article
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14 pages, 821 KB  
Article
Correlation Between Antimicrobial Consumption and Resistance in Klebsiella pneumoniae During the COVID-19 Pandemic Using Dynamic Regression Models: A Quasi-Experimental Epidemiological Time-Series Study
by Paul Laffont-Lozes, Florian Salipante, Paul Loubet, Catherine Dunyach-Remy, Jean-Philippe Lavigne, Albert Sotto and Romaric Larcher
Antibiotics 2025, 14(10), 1020; https://doi.org/10.3390/antibiotics14101020 - 14 Oct 2025
Viewed by 382
Abstract
Background/Objectives: The COVID-19 pandemic has been reported to impact antimicrobial consumption (AMC) and antimicrobial resistance (AMR) worldwide. We aimed to assess this correlation in Klebsiella pneumoniae before and during the COVID-19 pandemic and to estimate the burden of each antibiotic. Methods: [...] Read more.
Background/Objectives: The COVID-19 pandemic has been reported to impact antimicrobial consumption (AMC) and antimicrobial resistance (AMR) worldwide. We aimed to assess this correlation in Klebsiella pneumoniae before and during the COVID-19 pandemic and to estimate the burden of each antibiotic. Methods: We collected data on AMC of penicillins and beta-lactamase inhibitors (PBIs), anti-pseudomonal activity penicillins and beta-lactamase inhibitors (AAPBIs), cephalosporins, carbapenems, fluoroquinolones, aminoglycosides, and AMR in K. pneumoniae strains. The correlation between AMC and AMR was studied using dynamic regression models. Results: Overall, AMC of AAPBIs and fourth-generation cephalosporin increased, while fluoroquinolone consumption and AMR in the 2862 K. pneumoniae strains analyzed decreased. However, during the first year of the pandemic, we reported an increase in AMC and AMR. We found that 46% to 48% of the increase in cephalosporin, AAPBI, and fluoroquinolone resistance was explained by increased cephalosporin and fluoroquinolone consumption, 55% of the increase in PBI resistance was explained by increased PBI, cephalosporin, and fluoroquinolone consumption, and 58% of the increase in aminoglycoside resistance was explained by increased aminoglycoside consumption. Conclusions: During the COVID-19 pandemic, the increase in AMR in K. pneumoniae was correlated with the increase in AMC of several antibiotics, mainly cephalosporins and especially fluoroquinolones. Full article
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1 pages, 118 KB  
Correction
Correction: Pohlman et al. (2025). Interconnecting District and Community Partners to Improve School-Level Social, Emotional, and Behavioral Health. Behavioral Sciences, 15(9), 1225
by Kathryn B. Pohlman, Kayla Jones, Juan R. Lira, Jennifer Norton and Kelly Perales
Behav. Sci. 2025, 15(10), 1335; https://doi.org/10.3390/bs15101335 - 29 Sep 2025
Viewed by 166
Abstract
In the original publication (Pohlman et al [...] Full article
17 pages, 3974 KB  
Article
Electronic and Nuclear Subsystem Response in Hybrid Halide Perovskites Under γ-Irradiation
by Ivan E. Novoselov and Ivan S. Zhidkov
Nanomaterials 2025, 15(19), 1474; https://doi.org/10.3390/nano15191474 - 25 Sep 2025
Viewed by 346
Abstract
Lead halide perovskites, including single-cation (MAPbI3, FAPbI3, CsPbI3) and mixed-cation (Cs0.12FA0.88PbI3, Cs0.1MA0.15FA0.75PbI3) compositions, are promising for both space photovoltaics and γ-ray detection due [...] Read more.
Lead halide perovskites, including single-cation (MAPbI3, FAPbI3, CsPbI3) and mixed-cation (Cs0.12FA0.88PbI3, Cs0.1MA0.15FA0.75PbI3) compositions, are promising for both space photovoltaics and γ-ray detection due to their tunable optoelectronic properties. However, their response to high-energy radiation remains critical for reliable operation. We performed Monte-Carlo simulations using GEANT4 to investigate photon interactions (0.1–90 MeV) with perovskites of varying composition and thickness (1 cm to 1 μm). Results indicate that heavy atoms (Pb, I) dominate photoelectric absorption and scattering, broadly similar absorbed energies and event rates across compositions. Cs-containing perovskites exhibit slightly higher absorption and ionization, whereas FA- and MA-rich compositions show reduced photoelectric and Rayleigh scattering. Layer thickness strongly influences the radiation response: ultrathin films display fewer interactions with higher per-event energy, while millimeter-scale layers achieve efficient absorption and enable pair-production events at MeV energies. The sequence of dominant processes follows the expected energy dependence: photoelectric effect at low energies, Compton and Rayleigh scattering at intermediate energies, and pair production at high energies. These findings demonstrate that perovskite γ-interaction is primarily governed by heavy-atom content, with A-site cations fine-tuning the process balance, and that device performance for detection or photovoltaics depends critically on layer thickness. Full article
(This article belongs to the Section Nanoelectronics, Nanosensors and Devices)
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23 pages, 4793 KB  
Article
Undoped Polybenzimidazole Membranes Composited with CeP5O14 for Use in Hydrogen Fuel Cells at 200 °C
by Oksana Zholobko, Abdul Salam, Muhammad Muzamal. Ashfaq, Xiaoning Qi and Xiang-Fa Wu
Hydrogen 2025, 6(3), 70; https://doi.org/10.3390/hydrogen6030070 - 16 Sep 2025
Viewed by 1124
Abstract
Intermediate-temperature (IT) proton-exchange membranes (PEMs) play vital roles in hydrogen and direct liquid fuel cells, electrolyzers, and other electrochemical membrane reactors at elevated temperatures of higher than 150 °C. This article reports the fabrication and performance assessment of a type of new IT [...] Read more.
Intermediate-temperature (IT) proton-exchange membranes (PEMs) play vital roles in hydrogen and direct liquid fuel cells, electrolyzers, and other electrochemical membrane reactors at elevated temperatures of higher than 150 °C. This article reports the fabrication and performance assessment of a type of new IT polymer–inorganic composite (PIC) PEMs that were made of cerium ultraphosphate (CeP5O14-CUP) as the durable solid-state proton conductor and undoped polybenzimidazole (PBI) as the high-temperature (HT) polymeric binder. The proton conductivity and electrochemical performance of the PIC PEMs were characterized at 200 °C with varying membrane thickness, processing parameters, and operating conditions using a single-stack hydrogen fuel cell connected to a fuel cell test station. Experimental results show that the PIC membranes (with CUP of 75 wt.%) carried high mechanical flexibility and strength as well as noticeably reduced water uptake of 4.4 wt.% compared to pristine PBI membranes of 14.0 wt.%. Single-stack hydrogen fuel cell tests at 200 °C in a humidified hydrogen and air environment showed that the proton conductivity of the PIC PEMs was measured up to 0.105 S/cm, and the electrochemical performance exhibited its dependence upon the membrane thickness with the power density of up to 191.7 mW/cm2. Discussions are made to explore performance dependence and improvement strategies. The present study expects the promising future of the IT-PIC-PEMs for broad applications in high-efficiency electrochemical energy conversion and value-added chemical production at elevated temperatures of 200 °C or higher. Full article
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13 pages, 1060 KB  
Article
Interconnecting District and Community Partners to Improve School-Level Social, Emotional, and Behavioral Health
by Kathryn B. Pohlman, Kayla Jones, Juan R. Lira, Jennifer Norton and Kelly Perales
Behav. Sci. 2025, 15(9), 1225; https://doi.org/10.3390/bs15091225 - 9 Sep 2025
Cited by 2 | Viewed by 603 | Correction
Abstract
School districts face growing demands to address the academic, social, emotional, and behavioral health needs of all students, including meeting state mandates such as bullying prevention, suicide prevention, trauma response, and behavioral threat assessment. These needs have intensified since the COVID-19 pandemic, often [...] Read more.
School districts face growing demands to address the academic, social, emotional, and behavioral health needs of all students, including meeting state mandates such as bullying prevention, suicide prevention, trauma response, and behavioral threat assessment. These needs have intensified since the COVID-19 pandemic, often resulting in fragmented and inefficient planning. The Interconnected Systems Framework (ISF) offers a structure for uniting district and community efforts into a single, integrated system of support. While research has expanded on the effectiveness of the ISF and resources have defined installation steps, the process is often arduous and challenging to notice progress and maintain momentum in action planning. This study examines the use of the ISF District–Community Leadership Team (DCLT) Installation Progress Monitoring Tool as a means to provide district and community leaders with concrete data to monitor progress and inform evaluation and action plans. Findings highlight the tool’s potential to strengthen installation processes, promote data-informed decision-making, and improve alignment of resources to impact student and school outcomes. Full article
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12 pages, 1741 KB  
Article
Reactive Anti-Solvent Engineering via Kornblum Reaction for Controlled Crystallization in (FA0.83MA0.17Cs0.05)Pb(I0.85Br0.15)3 Perovskite Solar Cells
by Shengcong Wu, Qiu Xiong, Abd. Rashid bin Mohd Yusoff and Peng Gao
Inorganics 2025, 13(9), 295; https://doi.org/10.3390/inorganics13090295 - 1 Sep 2025
Viewed by 660
Abstract
Regulating the crystallization dynamics of perovskite films is key to improving the efficiency and operational stability of (FA0.83MA0.17Cs0.05)Pb(I0.85Br0.15)3 perovskite solar cells (PSCs). However, precise regulation of the crystallization process remains challenging. Here, [...] Read more.
Regulating the crystallization dynamics of perovskite films is key to improving the efficiency and operational stability of (FA0.83MA0.17Cs0.05)Pb(I0.85Br0.15)3 perovskite solar cells (PSCs). However, precise regulation of the crystallization process remains challenging. Here, we introduce a reactive anti-solvent strategy based on the Kornblum reaction to modulate crystallization via in-situ chemical transformation. Specifically, trans-cinnamoyl chloride (TCC) is employed as a single-component anti-solvent additive that reacts with dimethyl sulfoxide (DMSO) in the perovskite precursor solution. The resulting acylation reaction generates carbonyl-containing products and sulfur ions. The carbonyl oxygen coordinates with Pb2+ ions to form Pb–O bonds, which retard rapid crystallization, suppress heterogeneous nucleation, and facilitate the growth of larger perovskite grains with improved film uniformity. Additionally, the exothermic nature of the reaction accelerates local supersaturation and nucleation. This synergistic crystallization control significantly enhances the film morphology and device performance, yielding a champion power conversion efficiency (PCE) of 23.02% and a markedly improved fill factor (FF). This work provides a new pathway for anti-solvent engineering through in-situ chemical regulation, enabling efficient and scalable fabrication of high-performance PSCs. Full article
(This article belongs to the Special Issue New Semiconductor Materials for Energy Conversion, 2nd Edition)
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17 pages, 300 KB  
Article
Low Maternal Care and Protection and Body Image Dissatisfaction as Psychopathological Predictors of Binge Eating Disorder in Transitional-Age Youth
by Emanuela Bianciardi, Rossella Mattea Quinto, Ester Longo, Valentina Santelli, Lorenzo Contini, Alberto Siracusano, Cinzia Niolu and Giorgio Di Lorenzo
Nutrients 2025, 17(17), 2737; https://doi.org/10.3390/nu17172737 - 23 Aug 2025
Viewed by 996
Abstract
Background: Binge eating disorder (BED) frequently arises during the transitional age (18–25 years), a critical developmental period characterized by challenges in autonomy, identity formation, and interpersonal functioning. This study investigated psychopathological predictors of BED risk in this age group, with particular focus [...] Read more.
Background: Binge eating disorder (BED) frequently arises during the transitional age (18–25 years), a critical developmental period characterized by challenges in autonomy, identity formation, and interpersonal functioning. This study investigated psychopathological predictors of BED risk in this age group, with particular focus on parental bonding, attachment style, body dissatisfaction, alexithymia, and depressive symptoms. Methods: A total of 287 participants aged 18–25 years completed the Binge Eating Scale (BES), Beck Depression Inventory-II (BDI-II), Body Shape Questionnaire (BSQ), Toronto Alexithymia Scale (TAS-20), Attachment Style Questionnaire (ASQ), and Parental Bonding Instrument (PBI). Sociodemographic information and body mass index (BMI) were also collected. Results: Compared with non-BED risk groups, individuals at risk of BED exhibited significantly higher BMI, greater alexithymia, higher body dissatisfaction, more insecure attachment patterns, and lower recalled paternal and maternal care. Hierarchical binary logistic regression revealed that the final model explained 56.1% of the variance (Nagelkerke R2) and correctly classified 92.1% of cases. Significant predictors of BED included body dissatisfaction, elevated BMI, low maternal care, and low maternal protection. Conclusions: This study is the first to examine BED risk factors specifically during the transitional age. Findings indicate that body image dissatisfaction, higher BMI, and inadequate maternal emotional care and protection are salient predictors at this life stage. Preventive interventions should integrate parental psychoeducation, nutritional guidance, and therapeutic strategies addressing both eating disorder symptoms and attachment-related difficulties to reduce BED onset and improve psychosocial outcomes in emerging adults. Full article
17 pages, 2725 KB  
Systematic Review
Management and Outcomes of Non-Missile Penetrating Brain Injury Involving the Anterior Skull Base: A Case Report and Systematic Review
by Wojciech Czyżewski, Michał Szymoniuk, Jakub Litak, Krzysztof Kura, Klaudia Kuś-Budzyńska, Aleksandra Dryla, Jacek Baj, Kamil Torres and Grzegorz Staśkiewicz
J. Clin. Med. 2025, 14(16), 5731; https://doi.org/10.3390/jcm14165731 - 13 Aug 2025
Viewed by 919
Abstract
Introduction: Non-missile penetrating brain injury (PBI) involving the anterior skull base constitutes a rare subclass of traumatic brain injury in civilians. Management of this type of trauma is poorly described in the literature, with only case series and reports available. Materials and Methods: [...] Read more.
Introduction: Non-missile penetrating brain injury (PBI) involving the anterior skull base constitutes a rare subclass of traumatic brain injury in civilians. Management of this type of trauma is poorly described in the literature, with only case series and reports available. Materials and Methods: A systematic search was conducted across PubMed, Scopus, and Web of Science databases. The study included reports of adult patients with non-missile PBI with foreign bodies crossing the anterior skull base, published between the years 2000 and 2024. The patients were divided into three groups based on the entry point of foreign bodies: transorbital, transmental, and transnasal injuries. The obtained data were analyzed through descriptive statistics. A case report of a 20-year-old male following PBI involving the anterior skull base caused by suicidal self-shooting with a crossbow is presented. Results: A total of 17 articles reporting 40 patients and the current case were included. The mean age of the patients was 37.4 ± 13.1 years, and 92.7% of them were male. Transorbital injury was the dominant type of PBI (29 cases), followed by transmental injury (7 cases) and transnasal injury (5 cases). A total of 37 patients (90.2%) were managed operatively due to retained foreign bodies after PBI. Antibiotic prophylaxis was implemented in 33 cases (80.5%), mostly in transorbital (93.1%) and transnasal (100%) PBI. In seven reported cases, antiepileptic drugs were preventively administered. At the last follow-up, 18 patients (47.4%) did not fully recover neurological functions, with vision loss as the most common deficit. Conclusions: Management of non-missile PBIs involving the anterior part of the skull base is complex, challenging, and often requires a multidisciplinary team including neurosurgeons, ENT surgeons, ophthalmologists, and maxillofacial surgeons. In this type of traumatic brain injury, following proper management may lead to favorable outcomes with minimal neurological deficits. Full article
(This article belongs to the Section Brain Injury)
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18 pages, 2472 KB  
Article
Serum Metabolomic Signatures in Nonhuman Primates Treated with a Countermeasure and Exposed to Partial- or Total-Body Radiation
by Alana D. Carpenter, Yaoxiang Li, Benjamin E. Packer, Oluseyi O. Fatanmi, Stephen Y. Wise, Sarah A. Petrus, Martin Hauer-Jensen, Amrita K. Cheema and Vijay K. Singh
Metabolites 2025, 15(8), 546; https://doi.org/10.3390/metabo15080546 - 12 Aug 2025
Viewed by 683
Abstract
Background: Irradiation-induced injury is a common fallout of radiological/nuclear accidents or therapeutic exposures to high doses of radiation at high dose rates. Currently, there are no prophylactic drugs available to mitigate radiation injury as a result of exposure to lethal doses of [...] Read more.
Background: Irradiation-induced injury is a common fallout of radiological/nuclear accidents or therapeutic exposures to high doses of radiation at high dose rates. Currently, there are no prophylactic drugs available to mitigate radiation injury as a result of exposure to lethal doses of ionizing radiation. Gamma-tocotrienol (GT3) of vitamin E is a promising radioprotector under advanced development which has been tested for efficacy in both murine and nonhuman primate (NHP) models. Previously, we have demonstrated that GT3 has radioprotective efficacy in intestinal epithelial and crypt cells, and restores transcriptomic changes in NHPs with a supralethal dose of 12 Gy total-body irradiation (TBI). Methods: In this study, we evaluated the effect of 12 Gy partial-body irradiation (PBI) or TBI on metabolomic changes in serum samples and the extent to which GT3 was able to modulate these irradiation-induced changes. A total of 32 nonhuman primates were used for this study, and blood sample were collected 3 days (d) prior to irradiation, and 4 h, 8 h, 12 h, 1 d, 2 d, and 6 d post-irradiation. Results: Our results demonstrate that exposure to a supralethal dose of radiation induces a complex range of metabolomic shifts with similar degrees of dysregulation in both partial- and total-body irradiated animals. The C21-steroid hormone biosynthesis and metabolism pathway was significantly dysregulated in both PBI and TBI groups, with minimal protection afforded by GT3 administration. Conclusions: GT3 offered a differential response in terms of protected metabolites and pathways in either group that was most effective at the early post-irradiation time points. Full article
(This article belongs to the Section Advances in Metabolomics)
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13 pages, 1891 KB  
Article
Defect-Targeted Repair for Efficient and Stable Perovskite Solar Cells Using 2-Chlorocinnamic Acid
by Zhichun Yang, Mengyu Li, Jinyan Chen, Waqar Ahmad, Guofeng Zhang, Chengbing Qin, Liantuan Xiao and Suotang Jia
Nanomaterials 2025, 15(16), 1229; https://doi.org/10.3390/nano15161229 - 12 Aug 2025
Viewed by 892
Abstract
Metal halide perovskites have appeared as a promising semiconductor for high-efficiency and low-cost photovoltaic technologies. However, their performance and long-term stability are dramatically constrained by defects at the surface and grain boundaries of polycrystalline perovskite films formed during the processing. Herein, we propose [...] Read more.
Metal halide perovskites have appeared as a promising semiconductor for high-efficiency and low-cost photovoltaic technologies. However, their performance and long-term stability are dramatically constrained by defects at the surface and grain boundaries of polycrystalline perovskite films formed during the processing. Herein, we propose a defect-targeted passivation strategy using 2-chlorocinnamic acid (2-Cl) to simultaneously enhance the efficiency and stability of perovskite solar cells (PSCs). The crystallization kinetics, film morphology, and optical and electronic properties of the used formamidinium–cesium lead halide (FA0.85Cs0.15Pb(I0.95Br0.05)3, FACs) absorber were modulated and systematically investigated by various characterizations. Mechanistically, the carbonyl group in 2-Cl coordinates with undercoordinated Pb2+ ions, while the chlorine atom forms Pb–Cl bonds, effectively passivating the surface and interfacial defects. The optimized FACs perovskite film was incorporated into inverted (p-i-n) PSCs with a typical architecture of ITO/NiOx/PTAA/Al2O3/FACs/PEAI/PCBM/BCP/Ag. The optimal device delivers a champion power conversion efficiency (PCE) of 22.58% with an open-circuit voltage of 1.14 V and a fill factor of 82.8%. Furthermore, the unencapsulated devices retain 90% of their initial efficiency after storage in ambient air for 30 days and 83% of their original PCE after stress under 1 sun illumination with maximum power point tracking at 50 °C in a N2 environment, demonstrating the practical potential of dual-site molecular passivation for durable perovskite photovoltaics. Full article
(This article belongs to the Section Solar Energy and Solar Cells)
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18 pages, 5221 KB  
Article
New Isolates of Betachloroviruses Shed Light on the Diversity and Biological Complexity of an Unexplored Group of Giant Algal Viruses
by Júlia W. Souza, Lethícia R. Henriques, Roger M. Carlson, Bruna B. F. Botelho, João Victor R. P. Carvalho, João Pedro N. Santos, Eric R. G. R. Aguiar, Irina V. Agarkova, James L. Van Etten, David D. Dunigan and Rodrigo A. L. Rodrigues
Viruses 2025, 17(8), 1096; https://doi.org/10.3390/v17081096 - 8 Aug 2025
Viewed by 722
Abstract
The majority of giant algal viruses belong to the family Phycodnaviridae, class Algavirales, phylum Nucleocytoviricota. Among them, the genus Chlorovirus is the most studied, with three recognized groups based on genomics and host range, although many fundamental questions remain to [...] Read more.
The majority of giant algal viruses belong to the family Phycodnaviridae, class Algavirales, phylum Nucleocytoviricota. Among them, the genus Chlorovirus is the most studied, with three recognized groups based on genomics and host range, although many fundamental questions remain to be elucidated, particularly regarding their diversity. In this study, we focus on betachloroviruses, a poorly explored subgroup that infects the alga Micractinium conductrix Pbi. Here, we describe the isolation and genomic analysis of 11 new betachloroviruses from water samples collected in Nebraska, USA. With 25 fully sequenced genomes now available, we assessed the genomic diversity of these viruses. They have double-stranded DNA genomes ranging from 295 to 374 kbp, encoding hundreds of ORFs, of which a large number (~40%) lack known function. Comparative genomics and phylogenetic analyses revealed three species of betachlorovirus, each with high intra-species genomic identity. Notably, some isolates with over 99.5% genomic identity display markedly different plaque phenotypes, which led us to propose the use of the term genomovar among giant algal viruses, a concept potentially applicable to other giant viral groups yet to be explored. Altogether, this work advances our understanding of betachloroviruses and highlights the importance of linking viral genotype to phenotype, opening new avenues for exploring the diversity of giant algal viruses. Full article
(This article belongs to the Special Issue Cyanophage and Algal Virus)
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16 pages, 20542 KB  
Article
Establishment of Agrobacterium-Mediated Transient Transformation System in Sunflower
by Fangyuan Chen, Lai Wang, Qixiu Huang, Run Jiang, Wenhui Li, Xianfei Hou, Zihan Tan, Zhonghua Lei, Qiang Li and Youling Zeng
Plants 2025, 14(15), 2412; https://doi.org/10.3390/plants14152412 - 4 Aug 2025
Viewed by 817
Abstract
Sunflower (Helianthus annuus L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding. However, the current platforms for identifying gene function in sunflower is inadequate. The transient [...] Read more.
Sunflower (Helianthus annuus L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding. However, the current platforms for identifying gene function in sunflower is inadequate. The transient transformation system, which can rapidly validate gene function, shows promising prospects in research. In this study, we established an efficient transient expression transformation system for sunflower using three methods: Agrobacterium-mediated infiltration, injection, and ultrasonic-vacuum. The detailed procedures were as follows: Agrobacterium GV3101 carrying a GUS reporter gene on the pBI121 vector with an OD600 of 0.8 as the bacterial suspension and 0.02% Silwet L-77 as the surfactant were utilized in all three approaches. For the infiltration method, seedlings grown hydroponically for 3 days were immersed in a bacterial suspension containing 0.02% Silwet L-77 for 2 h; for the injection method, the same solution was injected into the cotyledons of seedlings grown in soil for 4 to 6 days. Subsequently, the seedlings were cultured in the dark at room temperature for three days; for the ultrasonic-vacuum method, seedlings cultured in Petri dishes for 3 days were first subjected to ultrasonication at 40 kHz for 1 min, followed by vacuum infiltration at 0.05 kPa for 5–10 min. Agrobacterium-mediated transient transformation efficiency achieved by the three methods exceeded 90%, with gene expression being sustained for at least 6 days. Next, we employed the infiltration-based sunflower transient transformation technology with the Arabidopsis stable transformation platform to confirm salt and drought stress tolerance of candidate gene HaNAC76 from sunflower responding to various abiotic stresses. Altogether, this study successfully established an Agrobacterium-mediated transient transformation system for sunflower using these three methods, which can rapidly identify gene function and explore the molecular mechanisms underlying sunflower’s resistance traits. Full article
(This article belongs to the Section Plant Cell Biology)
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13 pages, 4134 KB  
Communication
An Improved Agrobacterium-Mediated Transformation Method for an Important Fresh Fruit: Kiwifruit (Actinidia deliciosa)
by Chun-Lan Piao, Mengdou Ding, Yongbin Gao, Tao Song, Ying Zhu and Min-Long Cui
Plants 2025, 14(15), 2353; https://doi.org/10.3390/plants14152353 - 31 Jul 2025
Viewed by 2546
Abstract
Genetic transformation is an essential tool for investigating gene function and editing genomes. Kiwifruit, recognized as a significant global fresh fruit crop, holds considerable economic and nutritional importance. However, current genetic transformation techniques for kiwifruit are impeded by low efficiency, lengthy culture durations [...] Read more.
Genetic transformation is an essential tool for investigating gene function and editing genomes. Kiwifruit, recognized as a significant global fresh fruit crop, holds considerable economic and nutritional importance. However, current genetic transformation techniques for kiwifruit are impeded by low efficiency, lengthy culture durations (a minimum of six months), and substantial labor requirements. In this research, we established an efficient system for shoot regeneration and the stable genetic transformation of the ‘Hayward’ cultivar, utilizing leaf explants in conjunction with two strains of Agrobacterium that harbor the expression vector pBI121-35S::GFP, which contains the green fluorescent protein (GFP) gene as a visible marker within the T-DNA region. Our results show that 93.3% of leaf explants responded positively to the regeneration medium, producing multiple independent adventitious shoots around the explants within a six-week period. Furthermore, over 71% of kanamycin-resistant plantlets exhibited robust GFP expression, and the entire transformation process was completed within four months of culture. Southern blot analysis confirmed the stable integration of GFP into the genome, while RT-PCR and fluorescence microscopy validated the sustained expression of GFP in mature plants. This efficient protocol for regeneration and transformation provides a solid foundation for micropropagation and the enhancement of desirable traits in kiwifruit through overexpression and gene silencing techniques. Full article
(This article belongs to the Special Issue Plant Transformation and Genome Editing)
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