Search Results (344,733)

Prostate cancer (PCa) is the second most frequent tumor and the fifth leading cause of cancer-related death in men worldwide. PCa shows the largest clinical disparities across Asian, Caucasian, and African descendants among all cancer types, proving that the ethnic genetic background plays a significant role in PCa. Androgen Receptor (AR) gene malfunctioning represents the most prevalent cause of PCa. AR also displays a broad spectrum of genetic variability across ethnic backgrounds differently associated with cancer risk. We conducted a massive sequencing analysis of 15 genes highly relevant for PCa or the AR activation pathway in biopsies from 64 tumors and 36 benign prostate samples from Mexican patients. We identified 3414 genomic mutations and observed that AR, SPOP, TP53, FOXA1, and MTOR had the highest rate of pathogenic mutations in tumors, evidencing their relevance in PCa. AR showed 13 unique mutations, followed by SPOP (6), TP53 (5), FOXA1 (4), and MTOR (3). We discovered 19 novel mutations specific of Hispanic patients, a population only scarcely studied, thus adding critical information on the genetic diversity of the mutational landscape in genes key for PCa. We discuss the clinical relevance of these mutations and predict the structural consequences on the proteins. Mutations in FOXA1 showed significant negative association with patient survival and might be used as novel PCa markers, at least for Hispanic men. Full article

Chronic liver disease is closely associated with impaired cardiovascular function. Cardiac dysfunction is caused in part by oxidative stress and increased levels of proinflammatory and profibrogenic mediators in myocardial tissue. The present study aims to investigate the role of betaine in the modulation of MIF-mediated oxidative stress, inflammation, and fibrogenesis in heart during TAA-induced liver fibrosis in mice. The experiment is performed on wild-type and knockout MIF^−/− C57BL/6 mice (MIF^−/− group). They are randomly divided into groups: Control; Bet-group, received betaine (2% wt/v dissolved in drinking water); MIF^−/− mice group; MIF^−/−+Bet; TAA-group, treated with TAA (200 mg/kg b.w.), intraperitoneally, 3×/week/8 weeks); TAA+Bet; MIF^−/−+TAA, and MIF^−/−+TAA+Bet group. After eight weeks of treatment, animals are sacrificed and heart samples are taken to determine oxidative stress parameters, proinflammatory cytokines, profibrogenic factors, and histopathology of myocardial tissue. Our results suggest that MIF contributes significantly to lipid peroxidation of cardiomyocytes, as well as oxidative and nitrosative stress in myocardial tissue in mice with TAA-induced liver fibrosis compared to the control group. In addition, MIF was important for myocardial expression of the proinflammatory cytokines IL-6 and TNF as well as the profibrogenic mediators TGF-β1 and PDGF-BB in TAA-treated mice. Notably, betaine attenuated MIF effects in myocardial tissue reducing levels of MDA, AOPP, TNF, TGF-β1, PDGF-BB and increasing SOD and catalase activity in the coexistence of liver fibrosis. These results emphasize the potential of betaine as a therapeutic agent in mitigating MIF effects and demonstrate the need for further research into its optimal dosage and efficacy in preventing or slowing down cardiac dysfunction in patients with liver cirrhosis. Full article

With recent advancements in digital environments, user authentication is becoming increasingly important. Traditional authentication methods such as passwords and PINs suffer from inherent limitations, including vulnerability to theft, guessing, and replay attacks. Consequently, there has been a growing body of research on more accurate and efficient user authentication methods. One such approach involves the use of biometric signals to enhance security. However, biometric methods face significant challenges in ensuring stable authentication accuracy, primarily due to variations in the user’s environment, physical activity, and health conditions. To address these issues, this paper proposes a biometric-signal-based user authentication system using graph neural networks (GNNs). The feasibility of the proposed system was evaluated using an electromyogram (EMG) dataset specifically constructed by Chosun University for user authentication research. GNNs have demonstrated exceptional performance in modeling the relationships among complex data and attracted attention in various fields. Specifically, GNNs are well-suited for modeling user behavioral patterns while considering temporal and spatial relationships, making them an ideal method for adapting to dynamic and evolving user patterns. Unlike traditional neural networks, GNNs can dynamically learn and adapt to changes or evolutions in user behavioral patterns over time. This paper describes the design and implementation of a user authentication system using GNNs with an EMG dataset and discusses how the system can adapt to dynamic and changing user patterns. Full article

Martensitic stainless steel is a commonly used stainless steel, and is widely used in daily production and life, but its high content of alloy elements increases its cost. This study aimed to develop low-cost martensitic stainless steel with excellent seawater corrosion resistance by using Zr-Ti combined deoxidation with molten iron from low-priced laterite nickel ore as raw material, taking advantage of the corrosion-resistant elements Cr and Ni, abundant in laterite nickel ore. The characteristics of corrosion-active inclusions in steel, such as their density and electrostatic potential saturation-current density, were observed and studied using scanning electron microscopy and electrochemical testing methods. The intentionally added composite deoxidizing elements (Zr, Ti) form highly stable oxide particles at high temperatures. During the solidification of the molten steel and subsequent solid-state phase transformation, the highly corrosion-active MnS nucleates and disperses on the oxide particles already formed in the liquid phase, while TiN continues to precipitate and coat the MnS particles. This significantly reduces the saturation-current density of locally corrosion-active inclusions, resulting in a marked improvement in seawater corrosion resistance. Full article

Background: This study investigated the biomechanical and physiological demands of the hiking position in Laser sailing, a posture requiring sailors to extend their upper bodies outside the boat to counter wind-induced heeling. This study utilized a mixed-methods approach. Methods: Twenty-two experienced Laser sailors participated in both on-land and offshore assessments. The study combined subjective discomfort ratings, biomechanical measurements, digital human modeling, and muscle activation analysis to evaluate the effects of hiking during and after exertion. Results: A two-way ANOVA showed significant effects by body region and time. The quadriceps, abdominals, and lower back reported the highest discomfort. Key postural angles were identified, including knee and hip flexion, trunk inclination, and ankle dorsiflexion. Muscle activation analysis revealed the highest engagement in the rectus abdominis (46.1% MVC), brachialis (~45%), and psoas major (~41%), with notable bilateral asymmetries. The trunk region had the highest overall activation (28.7% MVC), followed by the upper limbs (~18.7%), while the lower limbs were minimally engaged during static hiking. Conclusions: On-water conditions resulted in greater variability in joint angles, likely reflecting wind fluctuations and wave-induced boat motion. Findings highlight the quadriceps, abdominals, and lower back as primary contributors to sustained hiking, while also emphasizing the importance of targeted endurance training and ergonomic equipment design. These insights can guide training, recovery, and ergonomic strategies to optimize performance and reduce injury risk in Laser sailors. Full article

Alpine meadow ecosystems are highly sensitive to global change, yet the response mechanisms of soil respiration (Rs) to nitrogen deposition remain unclear. This research employed a gradient nitrogen addition experiment (0, 5, 10, 15, 20 g·m⁻²·a⁻¹) in an alpine meadow ecosystem in Northwest China to determine the major factors regulating soil respiration responses. High nitrogen inputs (N15 and N20) significantly elevated Rs by 31.96% and 29.21% relative to the control (p < 0.05). Nitrogen addition significantly increased soil ammonium nitrogen (NH₄⁺-N) content, as well as the activities of cellobiohydrolase (CBH) and peroxidase (POD). Microbial community structure shifted with nitrogen addition, showing increased relative abundance of Actinobacteriota (14–25%) and Basidiomycota (13–26%). Functional prediction analysis revealed that high nitrogen treatments enhanced bacterial carbon metabolism functions such as fermentation and ureolysis, while enriching fungal functional guilds like Wood Saprotroph and Arbuscular Mycorrhizal fungi. Partial Least Squares Path Modeling (PLS-PM) indicated that nitrogen addition indirectly drives changes in Rs by regulating physicochemical factors (e.g., NH₄⁺-N), which subsequently influence microbial community composition, functional potential, and key enzyme activities. These findings elucidate the factors influencing soil respiration under varying nitrogen addition levels, providing a theoretical basis for assessing soil carbon cycling in alpine meadows under global change scenarios. Full article

This article examines the current statutory care and protection landscape in Aotearoa New Zealand (Aotearoa), focusing on the operations of Waikato-Tainui, a post-treaty settlement entity operating on behalf of the Waikato tribe (iwi), within this complex colonial context to safeguard and nurture mokopuna (descendants) and whaanau (families). Waikato-Tainui supports indigenous mokopuna within a fundamentally flawed settler-colonial care and protection system while concurrently reimagining an indigenous-led model rooted in ancestral wisdom and knowledge systems. Mokopuna Ora (Thriving descendants) is an indigenous whaanau-led and mokopuna-centred care and protection initiative that has been piloted, tested, researched, evaluated, and expanded over the past eleven years within the current settler colonial system. Drawing from deep empirical ancestral wisdom, the authors reimagine a new approach, building a roadmap for mokopuna and whaanau success. Ki Tua o Ngaku Mokopuna is presented as a cultural practice framework encapsulating Waikato ancestral wisdom and knowledge. While still in its early implementation stages, its development has been generations in the making, belonging to Waikato paa (communal meeting places) and hapuu (sub-tribes). Beyond a tool for frontline staff, this framework offers a vision, measures of success, and standards of excellence to inform theory and practice. This work addresses continuous indigenous resistance against negative colonial impacts, reflecting a shared indigenous experience and system of care and protection. In contemporary Aotearoa, the neo-colonial challenge is exacerbated by the current right-wing coalition Government and its ideological stance. The swift and extensive legislative reforms driven by harmful racist ideology are unprecedented, facilitating the exploitation of people, Papatuuaanuku (the earth mother), and te taiao (the natural world) for corporate gain and profit. Maaori tribes, organisations, sub-tribes, families, and individuals are actively countering these racist ideologies, legislations, strategies, policies, funding decisions, and operational practices. This ongoing colonial violence is met with the strength of ancestral knowledge and wisdom, envisioning a future where mokopuna thrive. The framework represents indigenous love, growth, prosperity, and abundance amidst enduring colonial harm and ideological warfare. Full article

Heavy metals, nitrogen, and phosphorus play a significant role in the marine ecosystem and human health. In this work, the concentrations of heavy metals, inorganic nitrogen, and phosphorus were determined to assess the distribution characteristics, risk levels, and possible sources in seawater from Taizhou Bay. The concentration ranges of Cu, Pb, Zn, Cd, Hg, As, ammonia, nitrate, nitrite, and phosphate were 1.87–3.65 μg/L, 0.10–0.95 μg/L, 2.98–16.80 μg/L, 0.07–0.38 μg/L, 0.011–0.043 μg/L, 0.93–2.06 μg/L, 0.011–0.608 mg-N/L, 0.012–0.722 mg-N/L, 0.001–0.022 mg-N/L, and 0.004–0.044 mg-P/L, respectively. The ecological risks were evaluated by the single factor index, Nemerow pollution index, and risk quotient. The results indicated that Taizhou Bay is not currently facing ecological risk related to heavy metals, nitrogen, and phosphorus, but the RQ values emphasized the urgency of strengthening continuous monitoring of As, Cu, and Zn. The results of Pearson’s correlation indicated that salinity and chemical oxygen demand had a significant impact on nitrogen and phosphorus but little impact on heavy metals. Principal component analysis was then applied to analyze the probable origins of heavy metals and inorganic pollutants, suggesting that these pollutants were mainly derived from human activities along the bay. Full article

Multidrug-resistant bacterial infections pose a severe global health threat, highlighting the urgent need for innovative therapeutic options beyond traditional antibiotics. Phage therapy, which employs bacteriophages to infect and eradicate pathogenic bacteria, specifically offers a promising solution. However, the lack of well-characterized therapeutic phages has limited their broader clinical use. A critical aspect of activating the lytic potential of dormant prophages involves the strategic manipulation of transcription factor binding sites (TFBS), which function as pivotal regulatory nodes governing the transition between lysogenic dormancy and lytic activation. Our platform utilizes advanced bioinformatics tools to accurately identify and analyze TFBS, facilitating the targeted redesign or replacement of these sites to disrupt host-mediated repression. By systematically simulating modifications of these regulatory ‘switches,’ our platform computationally predicts reduced repressor activity, suggesting the potential for prophage activation and bacterial cell lysis. This novel methodology not only broadens the spectrum of therapeutic bacteriophages but also establishes a basis for individualized phage-based therapies, presenting a robust strategy to address the escalating challenge of antibiotic-resistant infections. By enabling the precise identification and engineering of TFBS, our platform signifies a transformative advancement in phage biology, effectively bridging the divide between computational analysis and therapeutic application. Full article

The paper systematically investigated the flotation behavior and interaction mechanisms of andalusite and quartz under sodium dodecyl sulfonate (SDS) through integrated experimental and computational approaches, including zeta potential measurements, Fourier-transform infrared (FTIR) spectroscopy, Materials Studio (MS)-based quantum chemical calculations, and single-mineral flotation tests. The results of zeta potential and infrared spectroscopy analysis indicated that SDS underwent strong chemical adsorption on the surface of andalusite, while the adsorption effect on the surface of quartz was not obvious. MS calculations showed that the {100} surface energy of andalusite was the lowest, and it was the most important dissociation surface. After SDS was adsorbed on the {100} surface of andalusite, the aluminum atoms on the surface of andalusite lost electrons, resulting in a significant increase in the number of positive charges they carried. The activity of oxygen atoms was enhanced, while the number of charges carried by silicon atoms changed relatively little. It was indicated that SDS adsorbed the active sites of Al atoms on the surface of andalusite. The results of the pure mineral flotation test further verified the accuracy of the previous test results, indicating that andalusite and quartz had a good flotation separation effect under the SDS system. Full article

The objective of this study was to evaluate the effects of single versus split pre-harvest applications of aminoethoxyvinylglycine (AVG) on the concentrations of bioactive compounds and antioxidant activity in ‘Baigent’ apple fruit cultivated under anti-hail nets, assessed at harvest and after cold storage. The pre-harvest application of AVG in a single dose (125 mg L⁻¹) in ‘Baigent’ apple trees reduces the total antioxidant activity and concentration of anthocyanins and the total phenolic compound and chlorogenic acid in the fruit skin, both at harvest and after cold storage and reduces, in the skin, the concentrations of epicatechin at harvest and of quercetin after the cold storage. The parceled application of AVG (62.5 mg L⁻¹ + 62.5 mg L⁻¹) does not influence or present a less-pronounced negative effect on the total antioxidant activity and the contents of the total phenolic compound and anthocyanins in the fruit skin. Split AVG application can help maintain fruit quality during storage, providing a practical strategy for producers to optimize both marketable quality and nutritional value, potentially positively impacting commercial returns. Full article

Despite our preliminary research about the inductive effect of exogenous abscisic acid (ABA) on the weed-suppressive activity of rice in a hydroponic system, there is a lack of knowledge regarding the induction mechanism for ABA application to enhance the ability for weed control underground. Here, two pot experiments using rice–barnyard grass mixed culture were conducted to investigate the effects of exogenous ABA treatment on weed inhibition strategies in both allelopathic rice PI312777 (PI) and non-allelopathic rice Lemont (Le). The largest observed weed inhibition changes in the two rice accessions both occurred with the 9 μmol/L ABA treatment. ABA induction on PI significantly increases the inhibitory effect on the plant height of barnyard grass with root contact and root segregation by 25.7% and 19.1%, respectively, with 23.5% increases observed in Le rice with root contact and no significant increases in plants with root segregation with nylon mesh. ABA induction also significantly increased the root distribution in the soil of Le. Compared with the uninduced group, ABA treatment significantly elevated the total amounts of reversibly adsorbed phenolic acids in the two soil layers of PI and the irreversibly adsorbed phenolic acids in Le soil layers. Furthermore, exogenous ABA could change the bacterial composition in rhizosphere soil of the two rice accessions, with the change in the species composition in the rhizosphere soil of the allelopathic rice PI being greater. Importantly, the bacterial compositions (Anaerolineales, Bacteroidales, and Myxococcale) in the PI rhizosphere soil of rice induced by ABA were more related to the contents of reversibly adsorbed phenolic acids in the soil. However, the core bacterial compositions that promote plant growth (Sphingomonadales, Cyanobacteriales, and Rhizobiales) in the Le rhizosphere soil were more related to the contents of irreversibly adsorbed phenolic acids in the soil. These findings suggested that the ABA induction mainly changed root distribution and core bacterial compositions in Le to enhance resource competition, whereas it stimulated the release of reversibly adsorbed phenolic acids to modulate the specific bacterial compositions in rhizosphere soil of PI and to strengthen allelopathic effects. Full article

Restoration plantations in subtropical regions, often established with fast-growing tree species such as Acacia auriculiformis A. Cunn. ex Benth and Eucalyptus urophylla S. T. Blake, are frequently developed on highly weathered soils characterized by phosphorus deficiency. To investigate strategies for mitigating nutrient imbalances in such ecosystems, a long-term (≥13 years) fertilization experiment was designed. The experiment involved three fertilization regimes: nitrogen fertilizer alone (N), phosphorus fertilizer alone (P), and a combination of nitrogen and phosphorus (NP) fertilizers. The objective of this study was to investigate the effects of long-term fertilization practices on soil quality in subtropical plantations using a soil quality index (SQI). Consequently, all conventional soil physical, chemical, and biological indicators associated with the SQI responses to long-term fertilization treatments were systematically evaluated, and a principal component analysis (PCA) was conducted, along with a literature review, to develop a minimum dataset (MDS) for calculating the SQI. Three physical indicators (silt, clay, and soil water content), three chemical indicators (soil organic carbon, inorganic nitrogen, and total phosphorus), and two biological indicators (microbial biomass carbon and phosphodiesterase enzyme activity) were finally chosen for the MDS from a total dataset (TDS) of eighteen soil indicators. This study shows that the MDS provided a strong representation of the TDS data (R² = 0.81), and the SQI was positively correlated with litter mass (R² = 0.37). An analysis of individual soil indicators in the MDS revealed that phosphorus addition through fertilization (P and NP treatments) significantly enhanced the soil phosphorus pool (64–101%) in the subtropical plantation ecosystem. Long-term fertilization did not significantly change the soil quality, as measured using the SQI, in either the Acacia auriculiformis (p = 0.25) or Eucalyptus urophylla (p = 0.45) plantation, and no significant differences were observed between the two plantation types. These findings suggest that the MDS can serve as a quantitative and effective tool for long-term soil quality monitoring during the process of forest sustainable management. Full article

Plastic pollution is an increasingly pressing environmental concern due to its persistence in ecosystems. To address this issue, this study evaluates polyethylene biodegradation and bioelectricity generation using Aspergillus ochraceopetaliformis in microbial fuel cells (MFCs). Single-chamber MFCs were designed (three) with carbon and zinc electrodes, where the fungus was cultivated in a nutrient-rich medium to enhance its metabolic activity. Parameters such as pH, power density, and FTIR spectra were monitored to assess plastic biodegradation. The results demonstrated a significant reduction in polyethylene mass and structure, along with a maximum generation of 0.921 V and 4.441 mA on day 26, with a power density of 0.148 mW/cm² and a current of 5.847 mA/cm². The optimal pH for fungal activity in the MFC was recorded at 7.059. Furthermore, FTIR analysis revealed a decrease in peak intensity at 1470 cm⁻¹ and 723 cm⁻¹, indicating structural modifications in the treated plastics. Furthermore, microbial fuel cells connected in series successfully powered an LED bulb, generating a maximum voltage of 2.78 V. These findings confirm the feasibility of using Aspergillus ochraceopetaliformis for biodegradation and bioelectricity generation, although practical applications require further optimization of system conditions and improvements in long-term stability. This research contributes to the development of biotechnological strategies for plastic waste management, sustainable integrating approaches with energy potential. Full article

Marine plastic pollution has become a critical transboundary environmental issue, particularly affecting coastal regions with insufficient waste management infrastructure. This study applies a modified Lagrangian hydrodynamic model, TrackMPD v.1, to simulate the movement and accumulation of macroplastics in the West Africa Coastal Area. The research investigates three case studies: (1) the Liberia–Gulf of Guinea region, (2) the Mauritania–Gulf of Guinea coastal stretch, (3) the Cape Verde, Mauritania, and Senegal regions. Using both forward and backward simulations, macroplastics’ trajectories were tracked to identify key sources and accumulation hotspots. The findings highlight the cross-border nature of marine litter, with plastic debris transported far from its source due to ocean currents. The Gulf of Guinea emerges as a major accumulation zone, heavily impacted by plastic pollution originating from West African rivers. Interesting connections were found between velocities and directions of the plastic debris and some of the characteristics of the West African Monson climatic system (WAM) that dominates the area. Backward modelling reveals that macroplastics beached in Cape Verde largely originate from the Arguin Basin (Mauritania), an area influenced by fishing activities and offshore oil and gas operations. Results are visualized through point tracking, density, and beaching maps, providing insights into plastic distribution and accumulation patterns. The study underscores the need for regional cooperation and integrated monitoring approaches, including remote sensing and in situ surveys, to enhance mitigation strategies. Future work will explore 3D simulations, incorporating degradation processes, biofouling, and sinking dynamics to improve the representation of plastic behaviour in marine environments. This research is conducted within the Global Development Assistance (GDA) Agile Information Development (AID) Marine Environment and Blue Economy initiative, funded by the European Space Agency (ESA) in collaboration with the Asian. Development Bank and the World Bank. The outcomes provide actionable insights for policymakers, researchers, and environmental managers aiming to combat marine plastic pollution and safeguard marine biodiversity. Full article