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72 pages, 4452 KB  
Review
A New Frontier in Food Safety: Cold Plasma Strategies for Effective Control of Fungi and Mycotoxins
by Eva María Mateo, Fernando Mateo, Andrea Tarazona, María Ángeles García-Esparza, José Miguel Soria and Misericordia Jiménez
Toxins 2026, 18(6), 241; https://doi.org/10.3390/toxins18060241 (registering DOI) - 23 May 2026
Abstract
Mycotoxins are compounds produced by the secondary metabolism of certain fungi. These compounds contaminate foods worldwide and pose a severe threat to the health of humans and animals. They also cause huge economic losses. A plethora of methodologies, encompassing agricultural, biological, chemical, and [...] Read more.
Mycotoxins are compounds produced by the secondary metabolism of certain fungi. These compounds contaminate foods worldwide and pose a severe threat to the health of humans and animals. They also cause huge economic losses. A plethora of methodologies, encompassing agricultural, biological, chemical, and physical approaches, have been devised to curtail the presence of mycotoxins in food commodities. Among the physical processes, cold plasma (CP) has emerged as a useful technique for controlling the presence of toxigenic fungi in foods and for degrading the mycotoxins occurring in them without significantly affecting the quality and organoleptic properties of the treated commodities. The present review endeavors to demonstrate the efficacy of CP as a method of eradicating or reducing both the toxigenic mycobiota and the mycotoxins present in the most contaminated foods, including nuts, dried fruits, and cereal grains. The mechanisms of toxin degradation proposed by the different researchers are also examined and compared. Furthermore, the impact of the CP effect on the quality, sensorial characteristics, and toxicological properties of the treated food is thoroughly examined. Full article
(This article belongs to the Special Issue Mitigation and Detoxification Strategies of Mycotoxins: 2nd Edition)
31 pages, 31068 KB  
Article
Estimating the Impact of Agricultural Land-Use–Land-Cover Change on Riverbank Stability and Critical Inland Navigation Areas of the Danube River
by Maxim Arseni, Valentina-Andreea Calmuc, Madalina Calmuc, Laureana Odajiu, Silvius Stanciu and Puiu Lucian Georgescu
Earth 2026, 7(3), 85; https://doi.org/10.3390/earth7030085 (registering DOI) - 22 May 2026
Abstract
Intensive agriculture, deforestation, and frequent land-use changes contribute to increased soil erosion and sediment transport from both arable and non-arable lands into minor river channels. These factors directly and indirectly influence riverbank erosion and, in turn, sediment transport in rivers. Evidence on anthropogenic [...] Read more.
Intensive agriculture, deforestation, and frequent land-use changes contribute to increased soil erosion and sediment transport from both arable and non-arable lands into minor river channels. These factors directly and indirectly influence riverbank erosion and, in turn, sediment transport in rivers. Evidence on anthropogenic land-use/land-cover (LU-LC) change impact remains limited in both quantitative and spatial terms within the Danube River Basin. The study area includes research results from 17 locations concerning satellite-derived LU-LC changes along the Romanian sector of the Danube River, as well as validation results with particular highlighting on the Corabia area, Romania. According to results derived from combining LU-LC products based on Copernicus satellite data (comparing the years 2000 and 2018) and validated in the field through UAV flights conducted in 2025, the conversion of riparian vegetation into cultivated or uncultivated land accelerates bank failure. This is particularly evident where agricultural areas are located in the immediate vicinity of riverbanks. Such bank failures can be attributed to a reduction in root cohesion and a decrease in soil–bank structural stability. As a consequence, sediment delivery to the river channel increases via overland flow. The workflow proposed in this study offers a transferable and adaptable solution for areas with similar characteristics for a multitemporal approach regarding the influence of agricultural lands especially on sediment transport and riverbank erosion. Full article
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4 pages, 292 KB  
Editorial
Intelligent Equipment and Automation Technology in Farmland Production
by Mingzhuo Guo and Jiale Zhao
Agriculture 2026, 16(11), 1143; https://doi.org/10.3390/agriculture16111143 - 22 May 2026
Abstract
Agricultural production is undergoing a rapid transition from mechanized field operations to data-informed, perception-supported, and adaptive equipment systems [...] Full article
33 pages, 1802 KB  
Article
How Rural E-Commerce Shapes Agricultural Carbon Emissions: Evidence from a Quasi-Natural Experiment in China
by Jingbang Hu and Guojun Yin
Sustainability 2026, 18(11), 5251; https://doi.org/10.3390/su18115251 - 22 May 2026
Abstract
Rural e-commerce is reshaping agricultural markets, yet its environmental consequences remain insufficiently understood. This study examines how the Rural E-commerce Comprehensive Demonstration (RECD) program affects agricultural carbon outcomes in China. Using a balanced panel of 2152 counties from 2010 to 2022, we employ [...] Read more.
Rural e-commerce is reshaping agricultural markets, yet its environmental consequences remain insufficiently understood. This study examines how the Rural E-commerce Comprehensive Demonstration (RECD) program affects agricultural carbon outcomes in China. Using a balanced panel of 2152 counties from 2010 to 2022, we employ a multi-period difference-in-differences (DID) model to identify the effect of the RECD policy. The results show that the RECD policy significantly increases total agricultural carbon emissions. Evidence for production expansion and production restructuring suggests that improved market access and stronger price incentives encourage output expansion and a shift toward more market-oriented production, thereby raising aggregate emissions. At the same time, the RECD policy significantly reduces the carbon emission intensity and improves the carbon emission efficiency, indicating better carbon performance per unit of agricultural output. Further analysis shows that this dual result reflects the coexistence of efficiency gains and scale expansion, with the scale effect dominating the technical effect at the current stage. The emission-increasing effect is more pronounced in balanced agricultural areas, poverty-designated counties, counties with weaker initial e-commerce foundations, and counties with higher initial emission levels, while stronger environmental regulation and green technological innovation significantly mitigate this effect. In addition, the RECD policy generates spillover effects on neighboring counties within 50 km. These findings provide empirical evidence on the effects of the RECD policy on agricultural carbon emissions and offer policy guidance for integrating rural e-commerce policies with low-carbon agricultural transformation. Full article
(This article belongs to the Special Issue Integration of Digitalization and Green Economy)
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17 pages, 7244 KB  
Article
Remnants of Semi-Natural Habitats Sustain Nocturnal Lepidoptera Diversity Within a Mediterranean Vineyard System
by Giada Zucco, Sara La Cava, Marco Infusino and Stefano Scalercio
Land 2026, 15(6), 898; https://doi.org/10.3390/land15060898 (registering DOI) - 22 May 2026
Abstract
Agricultural intensification has led to landscape homogenization and the widespread loss of semi-natural habitats, contributing to biodiversity decline in agroecosystems. Semi-natural areas embedded within croplands may mitigate these effects by acting as reservoirs and steppingstones for species providing ecosystem services such as pollination. [...] Read more.
Agricultural intensification has led to landscape homogenization and the widespread loss of semi-natural habitats, contributing to biodiversity decline in agroecosystems. Semi-natural areas embedded within croplands may mitigate these effects by acting as reservoirs and steppingstones for species providing ecosystem services such as pollination. We assessed the role of remnants of semi-natural habitats in sustaining the diversity and abundance of nocturnal Lepidoptera within a Mediterranean vineyard landscape (southern Italy) using monthly light trap sampling over almost one year. Assemblages were compared between vineyards and adjacent semi-natural patches. Multivariate analyses revealed marked differences in community composition between semi-natural habitat types. Species richness was consistently higher in semi-natural habitats, even when represented by small residual patches. Vineyard assemblages were characterized by reduced richness and a predominance of generalist species with high dispersal ability, indicating a simplified community structure. Nevertheless, some taxa of biogeographical interest were recorded. These findings demonstrate the importance of semi-natural habitats in maintaining nocturnal Lepidoptera diversity in vineyard-dominated landscapes and support their integration into sustainable agricultural management to enhance biodiversity conservation and the provision of ecosystem services. Full article
(This article belongs to the Section Landscape Ecology)
38 pages, 2798 KB  
Review
Biochar Modulates the Dynamics of Legacy Nutrients in Enhancing Soil Health and Crop Productivity
by Manish Kumar, Shiv Bolan, Rakesh Kumar, Juhi Gupta, Dingjiang Chen, Hao Wu, Sarah Stackpoole, Nitika Chandel, Santanu Mukherjee, Manoj Chandra Garg, Srinithi Mayilswami, Kadambot H. M. Siddique and Nanthi Bolan
Land 2026, 15(6), 896; https://doi.org/10.3390/land15060896 (registering DOI) - 22 May 2026
Abstract
Most major crops in agricultural soils exhibit relatively low nutrient use efficiency for nitrogen (N), phosphorus (P), and potassium (K), often necessitating supplemental nutrient inputs to achieve sustainable yields. Furthermore, the increasing use of biowastes such as compost, manure, and biosolids, which frequently [...] Read more.
Most major crops in agricultural soils exhibit relatively low nutrient use efficiency for nitrogen (N), phosphorus (P), and potassium (K), often necessitating supplemental nutrient inputs to achieve sustainable yields. Furthermore, the increasing use of biowastes such as compost, manure, and biosolids, which frequently have nutrient ratios that do not match crop requirements, has contributed to excessive nutrient inputs and subsequent accumulation in soils. This situation has been further exacerbated by intensive farming practices involving multiple cropping cycles per season. Overuse of nutrients causes them to accumulate in the soil, creating a legacy nutrient pool. The application of biochar as soil amendment is considered a potential strategy to control legacy nutrients dynamics. The current review inspects the possible value of biochar in modulating legacy nutrient reserves in the soil, thereby increasing the bioavailability of nutrients and improving crop yield. This review discusses the search scope and synthesis approaches for the bibliometric methodological component through rigorous screening process (Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA)), focusing on journal articles published in last 20 years that specifically address legacy nutrient management. The significance of the economic and environmental effects of legacy nutrients and the insufficient knowledge of how biochar application influences nutrient dynamics in soil highlight the necessity for additional research to address current gaps. Full article
(This article belongs to the Section Land, Soil and Water)
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18 pages, 1807 KB  
Article
Biostimulation of Tomato Plants (Solanum lycopersicum L.) Using Fragmented Extracellular DNA from Clavibacter michiganensis
by Ireri Alejandra Carbajal-Valenzuela, Luz María Serrano-Jamaica, Lucía Vazquez, Gabriela Medina-Ramos and Ramón Gerardo Guevara-González
Plants 2026, 15(11), 1599; https://doi.org/10.3390/plants15111599 - 22 May 2026
Abstract
Extracellular DNA (eDNA) has gained attention as a danger signal between organisms because of the ecological implications of this mechanism and its great potential as a biological modulator in agriculture. Self-DNA and non-self DNA have been evaluated earlier, both as plant immune system [...] Read more.
Extracellular DNA (eDNA) has gained attention as a danger signal between organisms because of the ecological implications of this mechanism and its great potential as a biological modulator in agriculture. Self-DNA and non-self DNA have been evaluated earlier, both as plant immune system elicitors. Here we show the effect of eDNA extracted from the bacterial phytopathogen Clavibacter michiganensis applied to tomato plants in different concentrations (50, 100 and 150 µg mL−1). Monitoring morphology of the plants, spectrophotometric determinations and RT-qPCR assays showed a dose-dependent effect on plant growth and root development, activation of antioxidant enzymes such as catalase and superoxide dismutase, biosynthesis of secondary metabolites, including phenolic compounds and flavonoids, and differential expression of genes related to plant stress response, such as chalcone synthase and phenylalanine ammonia-lyase. Lower concentration treatments showed an increment in the variables as beneficial responses for agricultural practices, and the higher concentration (150 µg mL−1) showed reduced or no effects on the evaluated variables. This work represents a step forward in the development of effective and more sustainable agricultural technology in crop production. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
20 pages, 7337 KB  
Article
Vernacular Architecture and Spatial Memory: An Architectural Analysis of Kalif Structures in Rize/Pazar and Their Evaluation in Terms of Intangible Cultural Heritage
by Emre Pınar and Tunç Aslan Tülücü
Buildings 2026, 16(11), 2064; https://doi.org/10.3390/buildings16112064 - 22 May 2026
Abstract
This study examines the kalif structure, a unique and increasingly invisible component of the rural architecture in the Eastern Black Sea region that is currently under threat of extinction, along with the tradition of kalif-guarding integrated with this structure. Historically constructed to protect [...] Read more.
This study examines the kalif structure, a unique and increasingly invisible component of the rural architecture in the Eastern Black Sea region that is currently under threat of extinction, along with the tradition of kalif-guarding integrated with this structure. Historically constructed to protect agricultural production from wildlife, kalifs are not merely functional shelters but also multi-layered memory objects where collective solidarity and social interaction are reproduced. A qualitative research method was adopted for the study, utilizing literature review, on-site physical documentation, and technical analysis centered on Yücehisar village in the Pazar district of Rize. Within the scope of the research, the material use and construction techniques of kalifs are detailed from an architectural perspective, and these practices are evaluated through the lens of Intangible Cultural Heritage. The findings indicate that the loss of the physical presence of kalifs due to the transition from corn to tea cultivation and rural migration signifies the dissolution of a production-based culture of living. Consequently, the study reveals the critical importance of incorporating the kalif and the act of kalif-guarding into academic literature and cultural memory within the framework of Intangible Cultural Heritage standards to preserve local identity and rural memory. Full article
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15 pages, 742 KB  
Article
AIS-Based Seasonal Transformer Scheduling Using Real SCADA Load Data for Irrigation-Intensive Rural Grids
by Leyla Akbulut, Hasan Sh. Majdi, Fatma Özdemir, Atılgan Atılgan, Joanna Kocięcka and Daniel Liberacki
Energies 2026, 19(11), 2509; https://doi.org/10.3390/en19112509 - 22 May 2026
Abstract
Efficient electricity distribution in rural areas is strongly affected by seasonal agricultural energy demand, particularly in irrigation-intensive regions where electricity consumption increases substantially during summer periods. Conventional transformer operation strategies in such rural grids often fail to adapt to seasonal load variability, leading [...] Read more.
Efficient electricity distribution in rural areas is strongly affected by seasonal agricultural energy demand, particularly in irrigation-intensive regions where electricity consumption increases substantially during summer periods. Conventional transformer operation strategies in such rural grids often fail to adapt to seasonal load variability, leading to unnecessary idle operation, increased technical losses, and reduced infrastructure efficiency. Existing approaches generally rely on static assumptions or simulated data, limiting their ability to represent real irrigation-driven seasonal load asymmetry. To address this issue, this study proposes a data-driven multi-objective seasonal transformer scheduling framework using a bio-inspired Artificial Immune System (AIS) algorithm. The model was developed using two years of empirical hourly SCADA load data and transformer operation records obtained from a real 380/154 kV TEİAŞ transmission substation in Central Anatolia, Türkiye. Hourly SCADA measurements were used for seasonal load characterization and objective-function evaluation, while transformer scheduling decisions were defined at the seasonal operational level. The proposed AIS-based scheduling strategy reduced annual technical energy losses by approximately 5.4 GWh, decreased operational costs by 10.81 million TL (≈360,000 USD), and lowered carbon emissions by about 2270 metric tons of CO2 compared with conventional static transformer operation. The study presents a proof-of-concept framework integrating empirical SCADA measurements with AIS-assisted seasonal transformer scheduling for practical utility-scale operational planning in irrigation-dominated rural electricity networks. Full article
24 pages, 3017 KB  
Article
Preliminary Findings of a Novel Thermal Drone-Based and AI Approach to Sampling Mesopredator Behaviour and Habitat Use
by Katrine Møller-Lassesen, Esther Magdalene Ellersgaard Enevoldsen, Cino Pertoldi and Sussie Pagh
Drones 2026, 10(6), 401; https://doi.org/10.3390/drones10060401 - 22 May 2026
Abstract
Habitat selection is often activity-specific, as animals may use different environments depending on whether they are foraging, breeding, or moving through the habitat. Behavioural studies of nocturnal species are challenging, and conventional methods are limited in their applicability. In this study, we tested [...] Read more.
Habitat selection is often activity-specific, as animals may use different environments depending on whether they are foraging, breeding, or moving through the habitat. Behavioural studies of nocturnal species are challenging, and conventional methods are limited in their applicability. In this study, we tested a thermal drone in combination with Artificial Intelligence (AI) for focal animal sampling and habitat use of mesopredators. A drone mounted with a thermal video camera recorded the movements and behaviours of red foxes (Vulpes vulpes), European badgers (Meles meles), and Eurasian otters (Lutra lutra), while simultaneously geocoding their position. Additionally, we tested an AI-based analysis, LabGym for species and behaviour detection of video recordings. In Danish agricultural areas, both habitat separation and spatial overlap among the three mesopredators, were observed. Foxes showed a higher degree of versatility in both behaviour and habitat choice compared to badgers and otters. Otters were primarily found near water bodies, while badgers preferred foraging under tree cover and in meadows. The optimised LabGym achieved 80.4% mAP for species identification and successfully classified four behaviours with more than 80% accuracy. Using the thermal drone in combination with geolocation data and AI enables spatial mapping of mesopredator activities, adding valuable insights into predator ecology. Full article
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22 pages, 19396 KB  
Article
The Impact of Drought Events on Cropland Phenology and Vegetation Productivity in Northeast China (2001–2020)
by Zeyu Zhang, Xiaodong Na, Xubin Li, Sunai Ma and Yizhe Wang
Agronomy 2026, 16(11), 1031; https://doi.org/10.3390/agronomy16111031 - 22 May 2026
Abstract
Ongoing global climate change and intensified human activities have increased the frequency and intensity of droughts, posing a serious threat to global ecosystems and agricultural sustainability. However, the seasonally differentiated effects of droughts on cropland phenology and productivity, especially in Northeast China, remain [...] Read more.
Ongoing global climate change and intensified human activities have increased the frequency and intensity of droughts, posing a serious threat to global ecosystems and agricultural sustainability. However, the seasonally differentiated effects of droughts on cropland phenology and productivity, especially in Northeast China, remain insufficiently understood, limiting the assessment of agro-ecosystem vulnerability and the development of effective adaptation strategies. In this study, the standardized precipitation evapotranspiration index (SPEI) was used to assess the frequency and severity of extreme drought in Northeast China based on run theory. Cropland phenology parameters and productivity were derived from time-series MODIS normalized difference vegetation index (NDVI), and gross primary productivity (GPP) products, which were smoothed using a Savitzky–Golay (S–G) filter. Correlation analyses were conducted to examine regional associations between SPEI-defined drought conditions and cropland phenology and productivity. Results show that: (1) Drought events occurred frequently in the central and southern parts of Northeast China, particularly in the Songnen Plain (5.22 events per decade) and the Liaohe Plain (4.89 events per decade); (2) the Songnen Plain showed significant increases (Sen’s slope > 0, p < 0.05) across all drought metrics over 2001–2020, which coincided with LOS shortening (−0.18 d a−1) and GPP decline (−9.12 g C m−2 a−1); in contrast, the Sanjiang Plain exhibited slight declines (Sen’s slope, p > 0.05) in drought metrics, resulting in LOS lengthening (0.06 d a−1) and GPP increases (7.84 g C m−2 a−1); and (3) drought impacts were strongly season-dependent, with autumn droughts showing a stronger association with reductions in crop productivity in local areas of Northeast China. These findings highlight the need to account for crop responses to drought events, which is essential for developing measures to cope with drought and protecting regional food security. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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17 pages, 1076 KB  
Review
Occurrence of Trifluoroacetic Acid in Wine and Its Relevance for Dietary Exposure and Human Health: A Narrative Review
by Andrea Moscato, Paola Rapisarda, Margherita Ferrante and Maria Fiore
Toxics 2026, 14(6), 454; https://doi.org/10.3390/toxics14060454 - 22 May 2026
Abstract
Trifluoroacetic acid (TFA) is an ultrashort-chain perfluoroalkyl substance (PFAS) characterized by environmental persistence, water solubility, and a growing global presence, resulting primarily from the degradation of fluorinated compounds. Evidence suggests that plant-based foods may represent an underestimated exposure route, with wine emerging as [...] Read more.
Trifluoroacetic acid (TFA) is an ultrashort-chain perfluoroalkyl substance (PFAS) characterized by environmental persistence, water solubility, and a growing global presence, resulting primarily from the degradation of fluorinated compounds. Evidence suggests that plant-based foods may represent an underestimated exposure route, with wine emerging as a significant dietary source due to accumulation in soils, irrigation water, and plant uptake. This review provides an updated summary of the evidence on the environmental sources and temporal evolution of TFA in wine, its analytical detection, its contribution to dietary exposure, potential implications for human health, and current regulatory attention. A structured but non-systematic literature search was conducted using PubMed and Scopus, supplemented by European reports and monitoring data, and in accordance with SANRA guidelines. Evidence shows that TFA concentrations in wine derive from widespread environmental sources and have increased over time, from negligible levels before the 1970s to a marked increase in recent decades. Reported concentrations range from tens to several hundred µg/L, despite analytical challenges. Exposure estimates indicate that wine may contribute significantly to total dietary TFA intake in regular consumers. Although toxicological data suggest low acute toxicity, uncertainties remain regarding long-term exposure, and regulatory limits for TFA in foods and beverages are lacking. Full article
17 pages, 512 KB  
Review
Regenerative Agriculture Promotes Soil Health by Improving Soil Structure Through Organic Carbon Storage
by Ryusuke Hatano and Shinya Iwasaki
Agriculture 2026, 16(11), 1140; https://doi.org/10.3390/agriculture16111140 - 22 May 2026
Abstract
Soil degradation driven by inappropriate soil management is a serious global challenge, while climate change-induced yield declines are increasing the conversion of natural ecosystems to agricultural land. This review examines how soil structure influences soil health, focusing on organo-mineral complexes derived from microbial [...] Read more.
Soil degradation driven by inappropriate soil management is a serious global challenge, while climate change-induced yield declines are increasing the conversion of natural ecosystems to agricultural land. This review examines how soil structure influences soil health, focusing on organo-mineral complexes derived from microbial biomass and soil organic carbon-to-clay (SOC/Clay) ratio as an indicator of structural quality. Regenerative agriculture based on conservation farming practices helps mitigate SOC depletion and aligns with the nature-based solutions framework. In Hokkaido, Japan, 10 years of clean agricultural applications (cover crops and organic matter application) increased SOC storage in farmland affected by volcanic eruption. This was associated with improved bulk density, porosity, cation exchange capacity, and phosphate absorption capacity, indicating improved soil health. The increased SOC rose SOC/Clay ratio to levels comparable with unaffected farmland (≥1/13). When the SOC/Clay ratio exceeded 1/13 (soil carbon storage level of 30 t C/ha/15 cm), carbon sequestration rate became negative. This suggests that improved soil health and structural quality may promote carbon saturation and stimulate microbial decomposition of existing SOC. While the threshold for SOC/Clay ratio varies depending on soil type, vegetation type, climatic conditions, and land use, changes in the SOC/Clay ratio can provide insights into changes in soil health and structural quality. Full article
32 pages, 940 KB  
Review
Humic Substances and Plant Growth-Promoting Rhizobacteria (PGPR) as Biostimulants Against Plant-Parasitic Nematodes: Mechanisms, Synergistic Effects, and Applications
by Mehdi Beheshti, Lenka Demková and Lenka Bobuľská
Agronomy 2026, 16(11), 1029; https://doi.org/10.3390/agronomy16111029 - 22 May 2026
Abstract
Plant-parasitic nematodes (PPNs) rank among the most economically destructive soilborne pathogens worldwide, causing annual crop losses estimated at USD 125–175 billion. Traditional management of plant parasitic nematodes has depended significantly on synthetic nematicides; however, increasing regulatory constraints, environmental pollution, and the rise of [...] Read more.
Plant-parasitic nematodes (PPNs) rank among the most economically destructive soilborne pathogens worldwide, causing annual crop losses estimated at USD 125–175 billion. Traditional management of plant parasitic nematodes has depended significantly on synthetic nematicides; however, increasing regulatory constraints, environmental pollution, and the rise of resistant nematode populations have generated an urgent need for sustainable alternatives. Humic substances (HS), comprising humic acids, fulvic acids, and humins derived primarily from leonardite and lignite, represent biologically active components of soil organic matter. Their different functional groups, like carboxylic, phenolic, and carbonyl groups, have direct nematicidal and nematostatic effects by stopping eggs from hatching, slowing down juvenile development, and lowering infectivity. They also indirectly improve soil structure, nutrient bioavailability, and the composition of the rhizosphere microbiome. Plant growth-promoting rhizobacteria (PGPR), particularly Bacillus spp. and Pseudomonas spp., suppress PPN populations through antibiotic biosynthesis, cuticle-degrading hydrolytic enzymes, nematostatic volatile organic compounds, and elicitation of induced systemic resistance (ISR). This review methodically analyzes the individual and synergistic processes by which HS and PGPR inhibit PPNs and enhance plant growth. Humic compounds strongly promote PGPR rhizosphere colonization, augmenting microbial metabolic activity and bioinoculant stability, hence producing combinatorial suppressive effects unattainable by either input independently. The combined HS-PGPR approach is reliable and environmentally sustainable for comprehensive nematode control, requiring multidisciplinary research to achieve global sustainable agriculture. Full article
14 pages, 975 KB  
Review
Epigenetic Regulation of Salt Stress Responses in Tomato: From DNA Methylation to Stress Memory
by Chunrui Chen, Chao Li, Huihui Zhu and Jianli Yang
Horticulturae 2026, 12(6), 649; https://doi.org/10.3390/horticulturae12060649 - 22 May 2026
Abstract
Soil salinization is increasingly threatening global agricultural productivity and food security, currently affecting over 6% of the world’s land and one-third of irrigated areas. Tomato (Solanum lycopersicum L.), a major vegetable crop worldwide, exhibits moderate sensitivity to salinity, which limits both its [...] Read more.
Soil salinization is increasingly threatening global agricultural productivity and food security, currently affecting over 6% of the world’s land and one-third of irrigated areas. Tomato (Solanum lycopersicum L.), a major vegetable crop worldwide, exhibits moderate sensitivity to salinity, which limits both its yield and fruit quality. In recent years, epigenetic regulation has gained attention as a key mechanism enabling flexible and reversible control of gene expression without altering DNA sequences. This review synthesizes current knowledge on the epigenetic control of salt stress responses in tomato, focusing on three interconnected levels: DNA methylation dynamics, RNA-directed DNA methylation (RdDM), and histone modifications. We explore how DNA methyltransferases reshape the methylome under salinity, using examples such as PKE1 and SlGI to illustrate functional gene-body methylation. The RdDM pathway is discussed with emphasis on the unexpected role of SlAGO4A as a negative modulator of stress tolerance and the growing evidence for RdDM-mediated regulation of transcription factors. We also examine the balanced regulation of histone acetylation and deacetylation, highlighting the conserved role of GCN5 in maintaining cell wall integrity and the diverse functions of histone deacetylases, such as SlHDA1, SlHDA3, and SlHDA5, in stress adaptation. Additionally, insights from wild tomato species and grafting-induced epigenetic changes are presented, revealing new dimensions of stress memory. Collectively, these epigenetic mechanisms constitute a complex regulatory framework that integrates stress responses with growth and development, providing potential targets for epigenetic breeding of salt-tolerant tomatoes. Full article
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