Search Results (2,166)

Background: The peach allergen Pru p 7, a member of the Gibberellin-Regulated Protein (GRP) family, has emerged as a key marker of severe fruit-induced allergies. It is hypothesized to mediate cross-reactivity between fruit allergens and cypress pollen. Given the increasing prevalence of food allergies and the complex patterns of cross-sensitization, the role of Pru p 7 in clinical allergy diagnostics warrants further investigation. Objective: This study aims to characterize the sensitization profile to Pru p 7 in a cohort of patients with suspected fruit allergy and to assess its relationship with cypress pollen allergy, particularly to Cup s 7, a homologous GRP from Cupressus sempervirens. Methods: A retrospective analysis was conducted on 20 patients evaluated at the Allergy Unit of the Fondazione IRCCS Policlinico San Matteo. Specific IgE (sIgE) levels to peach extract, Pru p 7, and Cup a 1 (cypress extract) were assessed using the ImmunoCAP^® system (Thermo Fisher Scientific Inc., Waltham, MA, USA). Statistical associations between sensitizations were evaluated using chi-square tests and Spearman’s correlation. Results: Sensitization to peach extract, Pru p 7, and cypress pollen was detected in 38%, 30%, and 45% of patients, respectively. Significant associations were observed between peach and cypress (χ² = 8.80, p = 0.003), peach and Pru p 7 (χ² = 8.23, p = 0.004), and cypress and Pru p 7 (χ² = 6.55, p = 0.01). Notably, all patients sensitized to Pru p 7 also tested positive for both peach and cypress allergens, supporting the hypothesis of pollen–food cross-reactivity. Conclusions: Pru p 7 is a clinically relevant allergen that may account for severe allergic responses in patients not sensitized to classical peach allergens. Its cross-reactivity with Cupressaceae-derived GRPs, such as Cup s 7, highlights the importance of molecular diagnostics in evaluating food allergies, particularly in regions with significant exposure to cypress pollen. Full article

Currently, D-fructose (DF) is produced through enzymatic isomerization of beta-D-glucose (DG) under disadvantageous conditions (equilibrium conversion of 50%, costly separation, etc.). Alternatively, the two-step Cetus enzymatic process became a promising approach for producing high-purity DF. First, DG is oxidized to keto-glucose (kDG) using commercial pyranose 2-oxidase (P2Ox). To avoid the fast P2Ox inactivation by the in situ produced hydrogen peroxide, catalase is added to decompose this byproduct. The DG oxidation occurs with high conversion and selectivity, leading to kDG free of allergenic aldose compounds. Then, kDG is reduced to DF by using the NADPH cofactor and aldose reductase (ALR). This study aims to evaluate the continuous in situ regeneration of NADPH at the expense of formate decomposition in the presence of formate dehydrogenase (FDH). By adopting a kinetic model from literature, this in silico analysis determines the optimal operation of a batch reactor (BR) used in the Cetus second step to maximize the DF production and minimize the consumption of costly NADPH. Compared to its simple operation, the optimized BR with cofactor regeneration reported a 25% lower NADPH consumption, though the amount of the processed substrate is ca. 3× higher. Also, the costly enzymes (ALR, FDH) consumption is 2× smaller. Full article

Background: Food allergies are chronic diseases that compromise quality of life and can be potentially fatal due to anaphylaxis. The WHO estimates a 1–11% global prevalence, which has been increasing in recent years. They are considered, along with obesity, to be the two noninfectious pandemics. The WHO databases (WHO/IUIS) contain 403 food allergens, most of which have been reported from North America (Canada and the USA), Europe, and Asia, while reports of allergens from Latin America are scarce. Allergies have population and geographical specificities; therefore, identifying the main clinically relevant food allergens and potential new, undescribed components affecting Latin America is essential. This work aims to contribute to this field. Methods: we gathered data from 16 allergic Mexican pediatric patients to fruits from the Rosaceae (pear and peach) and Musaceae (banana) families, as well as an allergic adult to Lauraceae (avocado). These fruits are prevalent allergens in Latin America. Results: the data suggest that patients reacted to 20 different allergenic proteins reported in different allergen databases. Furthermore, we identified 16 previously unreported immunoreactive proteins, suggesting their potential role as new allergens. Conclusion: this preliminary work is particularly relevant, as it can influence the specific diagnosis of allergens most frequently affecting the pediatric population. Full article

Glycosylation of many proteins has been revealed to be closely related to food allergy, and screening and structural analysis of related glycoproteins are essential for studies in this important area. Gly m Bd 30K is one of the major allergens that exist in soybeans. N-Glycans of the Gly m Bd 30K influenced the immunoreactivity and antigen-presenting efficiency. In this paper, soybean allergen glycoprotein Gly m Bd 30K was used as the research object. It was separated and purified by the combination of isoelectric point and Sepharose CL-6B gel. The glycoprotein was analyzed and identified by SDS-PAGE and MALDI-TOF MS. The N-glycans of Gly m Bd 30K glycoprotein were released and labeled by a newly developed one-pot method, and qualitatively and quantitatively analyzed by ESI-MSⁿ and HILIC-UV-MS/MS. The results showed that the purity of Gly m Bd 30K glycoprotein was 95%, and the relative molecular mass was 33,923 Da. The Gly m Bd 30K glycoprotein contained a total of six kinds of glycans, including two types: oligo-mannose type (4.3%) and paucimannose type (95.7%). The paucimannose modified with core α-1,3-fucose and β-1,2-xylose accounted for 92.87%. This study provides quality-reliable materials for the follow-up study of glycan sensitization and also provides a theoretical basis for the in-depth study of the specificity and biological function of the antigenic determinant of Gly m Bd 30K glycoprotein. Full article

The number of food allergies (FAs) is growing, particularly in industrialized countries, resulting in discomfort and a reduction in the quality of life of those who suffer from these allergies. Despite numerous epidemiological studies on FAs worldwide, the different approaches used constitute a limitation for an accurate prevalence of these disorders. The onset of FAs is multifactorial, since it depends on lifestyle, habitat, diet, and particularly on the diversity of gut microbiota. In fact, they occur in conditions of gut dysbiosis, an increase of the inflammatory state, an alteration of gut permeability, and an impairment of the resident immune cells, thus leading to a disruption and/or lack of tolerance towards food allergens. It is important to emphasize that the reduction of some bacteria leads to a decrease in their metabolite production, which, in turn, is crucial for the correct performance of immune responses and to ensure tolerance against allergens. Therefore, various strategies such as pre- and probiotic administration must be employed to avoid or prevent the onset of FAs. Full article

The growing global demand for alternative, sustainable protein sources has increased interest in edible insects, of which the domestic cricket (Acheta domesticus Linnaeus) is one of the most studied and exploited species. Crickets offer a rich source of protein, essential amino acids, and micronutrients, and provide significant benefits for environmental sustainability. This in-depth review, based on recent literature, examines the nutritional composition, developmental stages, and optimal housing conditions of crickets, with a focus on their use in the food industry. It also examines the technological challenges and legal frameworks of cricket farming, including feeding strategies and climate control, as well as the regulations governing insect-based foods. It also addresses potential risks, such as allergenic reactions and concerns related to chitin, as well as the role of crickets in the circular economy. The study outlines key challenges and prospects in insect production for food and feed and identifies priorities for future research. Our research discusses the legal background and highlights current findings related to entomophagy. This article presents an in-depth review of the nutritional value, farming conditions, food applications, and regulatory landscape for crickets as food. It also explores the technological challenges and the role of crickets in sustainability. Full article

Pistachio (Pistacia vera) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters must be carefully monitored by the operator, and sample preparation must be carried out without any modification in parameter values, under strictly controlled conditions. Good reproducibility was achieved for pistachio detection, while ongoing investigations should be carried out to overcome existing constraints for cashew. The LC-MS/MS method described in this work is a discriminatory method suitable for official food allergen control to selectively differentiate pistachio from cashew allergens, overcoming the limitations of PCR and ELISA when cross-reactivity occurs. It represents a validated tool for pistachio detection and a promising approach toward improving cashew allergen analysis. Full article

The prevalence of food allergies has been steadily increasing in recent years. β-lactoglobulin (β-LG), the main allergenic protein of milk and dairy allergies, is more commonly observed in infants and children. In this study, a β-LG-specific aptamer was selected using the combinatorial chemistry process known as systematic evolution of ligands by exponential enrichment (SELEX), and a quartz crystal microbalance with dissipation monitoring (QCM-D)-based aptasensor was developed using a novel surface functionalization technique, which mimics an artificial cell membrane on the QCM-D sensor surface, creating a physiologically relevant environment for the binding of the target to the sensor. Through SELEX combined with next-generation sequencing (NGS), the aptamer Apt 356 was identified. Its binding to β-LG was confirmed via dot blot analysis. The selected Apt 356 was then used for the development of a QCM-D-based sensor. To fabricate the sensor, the quartz surface was functionalized with a supported lipid bilayer (SLB). The β-LG-specific aptamer was immobilized onto this SLB. The results demonstrated that the QCM-D system allows real-time observation and evaluation of the binding of β-LG. While there have been some studies on aptasensors for the β-LG protein, to the best of our knowledge, this is the first QCM-D-based aptasensor developed specifically for β-LG protein detection. Full article

Based on field studies, this study contributes the new physical data of winter indoor thermal conditions of the indoor spaces with four different dust mite allergen levels in New Zealand houses. This study provides a new method to identify the relationships between indoor thermal conditions and indoor dust mite allergen levels. This study found that the indoor mean relative humidity (RH) close to the floor must be controlled below 70%, and there must be less than 30% of time in winter when indoor RH close to the floor is higher than or equal to 75% to maintain indoor dust mite allergens at an undetectable level; and the indoor mean RH close to the floor must be controlled below 75%, and there must be less than 50% of time in winter when indoor RH close to the floor is higher than or equal to 75% to maintain indoor dust mite allergens at a low (acceptable) level. This study also identified the relationship between indoor thermal conditions for dust mites to thrive and for mould spores to germinate. This study provides a strategy or guideline for preventing indoor allergies related to dust mites and mould under the temperate climate zone; the winter is mild and humid. Full article

Extracts used for allergen sensitization testing in humans are heterogeneous in composition and may contain low levels of important allergens. In contrast, there is scant information on the variability of veterinary allergen extracts. We selected two batches of extracts of Dermatophagoides farinae house dust mites from each of three different manufacturers. We observed a 3.4-fold difference in total protein content between the two extracts with the lowest and highest amounts of allergens. After electrophoresis, the band migration patterns were found to be similar between the two batches of extracts from two manufacturers and dissimilar in the third; the migration profiles were heterogeneous between manufacturers. The six extracts did not contain the same concentrations of Der f 1 and Der f 2, with fold changes between the highest and lowest concentrations measured to be 14.3× for Der f 1 and 8.0× for Der f 2. This allergen composition heterogeneity resulted in differences in ELISA seropositivity when allergic dogs had low serum concentrations of IgE against Der f 1 and Der f 2. Our findings demonstrate that, like in human allergology, allergen extracts for veterinary use are also heterogeneous in their protein content and allergen composition. This heterogeneity can impact results when determining allergen sensitizations using crude extracts. Full article

Background: Diagnosing food allergy (FA) typically involves a detailed clinical history and confirmation of allergen-specific IgE. Oral food challenges (OFCs) remain the gold standard in FA diagnosis. This study aimed to present our experience in performing OFCs in pediatric patients with particular focus on challenges performed with cow’s milk and hen’s egg. Methods: We conducted a retrospective analysis of 205 OFCs. Clinical data were evaluated and multiple logistic regression was used to identify associations between challenge outcomes, reaction severity, and comorbidities. Results: The mean age of patients was 5.7 ± 3.1 years, with 135 (65.9%) being male. The tested foods included cow’s milk protein (CMP, 103 challenges; 50.2%), hen’s egg white protein (HEWP, 84; 41.0%), peanuts (3; 1.5%), tree nuts (4; 2.0%), gluten (3; 1.5%), hen’s egg yolk (4; 2.0%), and other foods (4; 2.0%). The overall OFC failure rate was 32.2%, and five challenges (2.4%) yielded inconclusive results. The median cumulative reactive dose was 0.27 g for baked CMP and 0.58 g for baked HEWP. Most failed OFCs involved mucocutaneous symptoms (44 cases; 66.7%). Severe multisystemic reactions occurred in four patients (2.0%), all of whom required epinephrine (6.1% of positive challenges). An increased risk of OFC failure was associated with asthma (p = 0.028; 95% CI: 0.07–1.27) and multi-food allergy (p = 0.021; 95% CI: 0.14–1.67). Additionally, the coexistence of asthma and a prior history of anaphylaxis to any food was related to OFC failure (p = 0.049; 95% CI: 0.01–2.19), as was the combination of multi-food allergy and previous anaphylaxis (p = 0.043; 95% CI: 0.03–1.70). Receiver operating characteristic (ROC) curve analysis was utilized to predict outcomes of OFCs to baked milk and baked egg and determined a specific IgE (sIgE) cutoff level of 58.1 kU/L for baked milk challenges (AUC: 0.77; sensitivity: 0.588; specificity: 0.882), and 11.3 kU/L for baked egg challenges (AUC: 0.66; sensitivity: 0.692; specificity: 0.607). Conclusions: Our findings confirm that OFCs are a safe and effective tool for diagnosing FA in children. With appropriate patient selection, the risk of severe reactions remains low. Nonetheless, comorbidities such as asthma and multi-food allergy are associated with an increased likelihood of OFC failure. Full article

The growing demand for sustainable protein sources has boosted interest in Andean pseudocereals, particularly quinoa (Chenopodium quinoa), cañihua (Chenopodium pallidicaule), and kiwicha (Amaranthus caudatus), due to their complete nutritional profile, high digestibility, and low allergenic potential. Their inclusion in vegetarian and vegan diets represents a viable alternative that can replace animal proteins without compromising on nutritional quality. This study presents a critical review of indexed scientific literature analyzing essential amino acid composition, protein quality values—such as PDCAAS (Protein Digestibility-Corrected Amino Acid Score) and DIAAS (Digestible Indispensable Amino Acid Score)—and the impact of various processing technologies on the functionality of Andean proteins. Results show that these grains contain between 13 and 18 g of protein per 100 g of dry product and provide adequate levels of lysine, methionine, and threonine, meeting FAO (Food and Agriculture Organization) requirements for adult nutrition. Processes such as germination, fermentation, enzymatic hydrolysis, and extrusion have demonstrated improvements in both amino acid bioavailability and functional properties of proteins, enabling their application in gluten-free breads, meat analogs, and functional beverages. Furthermore, emerging strategies such as nanotechnology, bioactive peptide generation, and gene editing via CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)—a precise genome editing tool—open new possibilities for enhancing the nutritional and functional value of pseudocereals in the food industry. Taken together, the findings consolidate the strategic role of Andean grains as key ingredients in the development of sustainable, functional, and plant-based foods. Full article

Background: Due to the new COVID-19 vaccine types used in the COVID-19 vaccination program, significant levels of uncertainty and vaccine hesitancy arose due to fears about anaphylactic reactions, especially in patients with allergies. This study aimed to analyze how patients with significant allergies receiving specific immunotherapy (SIT) reacted to COVID-19 vaccines in real life. Methods: We assessed 57 patient charts of individuals with allergies receiving SIT for documented allergies, for their comorbidities, total IgE and tryptase levels, and Hospital Anxiety and Depression Scale (HADS) scores. Questionnaires regarding COVID-19 vaccination status and reported adverse reactions were analyzed. Results: Patients were more frequently male (58%) and had a mean age of 43 years, and the majority (60%) had already experienced an anaphylactic reaction in the past, most commonly to the allergen of the current SIT. In total, 93% received COVID-19 vaccinations. More than half of the patients (57%) reported at least one adverse reaction after COVID-19 vaccination. Out of these patients, 97% reacted in an unspecific way, with symptoms of general illness. One potential allergic reaction, classified as a grade 1 anaphylactic reaction, was documented. The patient with the potential allergic reaction was significantly more concerned before receiving the vaccination and had experienced possible allergic reactions to other vaccinations in the past. The remaining patients with unspecific reactions after vaccination had also experienced such adverse reactions in the past to other vaccinations. Antihistamine premedication was associated with fewer unspecific reactions after COVID-19 vaccination. Conclusions: Vaccinations in patients with significant allergies and prior anaphylactic reactions are, overall, safe. Prior allergic reactions after other vaccinations could be a potential risk for reaction after COVID-19 vaccinations, whereas antihistamine intake could be beneficial in reducing side effects after COVID-19 vaccinations in patients with allergies. Prospective studies on this topic are needed. Full article

Matrix metalloproteinases (MMPs) are a major group of proteases known to regulate the turnover of the extracellular matrix (ECM). We observed that induced MMP-12 promotes eosinophilic inflammation-related epithelial cell mesenchymal transition (EMT), bronchial fibrosis, and airway obstruction in an allergen-exposed mouse model of chronic airway diseases in allergen-exposed mice and in airway-specific CC10-IL-13-overexpressed mice. Our histological analysis showed that the parabronchial and perivascular accumulation of eosinophils, fibroblasts, and collagen is significantly decreased in MMP-12^−/− allergen-exposed mice and airway-specific rtTA-MMP-12^−/−CC-10-IL-13-overexpressed mice compared to allergen-exposed wild-type mice and rtTA-CC10-IL-13-overexpressed mice. ELISA and Western blot analyses validated these histological findings, demonstrating that EMT and profibrotic protein levels were significantly decreased in allergen-challenged MMP-12^−/− mice and rtTA-MMP-12^−/−CC10-IL-13-overexpressed mice in comparison to the allergen-exposed wild-type mice and rtTA-CC10-IL-13-overexpressed mice. In addition, we also observed that allergen-challenged MMP-12^−/− mice have improved resistance and compliance compared to allergen-challenged wild-type mice. Most importantly, we show that treatment with MMP-12 inhibitors (PF-00356231 and MMP408) restricts the induction and progression of bronchial fibrosis and airway restrictions in allergen-exposed mice and airway-specific rtTA-CC10-IL-13 mice compared to the respective control mice. Taken together, the novelty of these findings lie in the fact that induced MMP-12 regulates eosinophilic inflammation-induced bronchial fibrosis and associated airway restriction, which may be reduced by treatment with MMP-12 inhibitors. Full article

Allergic contact dermatitis (ACD) is a frequent inflammatory skin disease that evolves upon exposure to contact allergens in sensitized individuals. Both the adaptive and innate immune system play pivotal roles in the pathogenesis of ACD. While the importance of T cells is undisputed, the relevance of B lymphocytes is less clear. The published data support a critical role for NFATc1 in B cell activation. Therefore, we investigated the impact of NFATc1 on B cell function during murine contact hypersensitivity (CHS), the mouse model for human ACD. Compared with wild-type mice, B cell-specific ablation of NFATc1 (Nfatc1^f/f x mb1-cre) resulted in significantly diminished CHS responses measured by ear thickness (0.81 ± 0.02 mm vs. 0.48 ± 0.02 mm (p = 0.0007)) to the obligate contact allergen 2,4,6-trinitrochlorobenzene, accompanied by a marked increase in the frequency of IL-10-producing regulatory B cells. Flow cytometric analysis showed that IL-4- and IL-17-producing CD4⁺ T cells were reduced, while IFN-γ-producing CD4⁺ T cells were marginally increased in Nfatc1^f/f x mb1-cre mice. In conclusion, NFATc1 mediates CHS responses by modulating the development of IL-10-producing B cells. These findings support the compelling notion that targeting NFATc1 may represent a potential therapeutic strategy for allergic responses. Full article