Search Results (593)

Caffeoylquinic acids (CQAs), a class of phenolic acid metabolites widely distributed in plants, encompass 15 positional isomers from mono- to tetra-esters, with 5-O-caffeoylquinic acid (5-CQA) as the predominant form. The biosynthesis of 5-CQA from phenylalanine proceeds through five primary pathways, which are finely regulated by environmental, hormonal, and transcription factors from families such as MYB, WRKY, and bHLH. These regulators control 5-CQA synthesis by binding specifically to the promoter regions of key structural genes, including PAL, 4CL and HCT/HQT. Subsequently, 5-CQA serves as a central precursor for the biosynthesis of other CQAs. In terms of bioactivity, CQAs possess remarkable pharmacological activities, encompassing antioxidant, antimicrobial, anti-diabetic, anti-inflammatory and anti-tumor properties. For instance, anti-inflammatory effects are demonstrated by the ability of 5-CQA to reduce key pro-inflammatory cytokines (e.g., TNF-α and IL-1β) and downregulate the TLR4/NF-κB pathway. The synergistic action of 5-CQA with ultraviolet-A reduced succinate-coenzyme Q reductase activity by approximately 72%, highlighting its potential to disrupt bacterial metabolism and combat antibiotic resistance. Furthermore, 3,4,5-triCQA exhibits potent anti-influenza virus activity, potentially through a mechanism distinct from existing neuraminidase inhibitors. Beyond medicine, CQAs show promise in light industry. They serve as antibiotic alternatives in livestock feed to enhance gut health, extend food shelf life through their antioxidant activity, and function as active ingredients in UV-protective skincare formulations. CQAs also enhance plant stress tolerance to cold, arsenic, and pests by mechanisms such as scavenging reactive oxygen species and inhibiting pest mobility. While this review consolidates progress in the biosynthesis and bioactivity of CQAs specifically with caffeoyl substituents, future efforts should leverage modern biotechnological tools and interdisciplinary approaches to bridge critical knowledge gaps in their biosynthesis, transport, and clinical translation. Full article

Sustainability of animal production requires reducing reliance on soybean meal by identifying viable alternative protein sources. Within the framework of the Italian Agritech National Research Center, seven Italian research groups collaborated to evaluate unconventional feed ingredients and their effects on animal performance and product quality. Alternative legume seeds (peas, chickpeas, faba bean, and lupins) can partially or completely replace soybean meal without impairing productivity, while enhancing product health value and shelf-life through bioactive compounds. Microalgae (Chlorella, Spirulina) improved carotenoid content, antioxidant activity, fatty acid profile, and cholesterol levels in poultry products, with limited effects in pigs. Insects supported optimal growth in fish at 25–30% inclusion, whereas maximum recommended levels are 15% in broilers and 24% in laying hens to sustain growth, egg production, and quality. Camelina by-products are suitable for poultry diets at up to 5–10%, beyond which performance declines. Whole-plant soybean silage, tef (Eragrostis tef), and triticale–lupin intercropping represent promising protein-rich resources for ruminants, provided diets maintain balanced protein-to-energy ratios, adequate fibre characteristics, and appropriate harvest timing under drought-prone conditions. Collectively, these findings highlight the potential of diverse protein sources to improve the sustainability of livestock systems while preserving productivity and enhancing the nutritional quality of animal-derived foods. Full article

Rapid population growth has increased the demand for sustainable systems to produce protein-rich foods. Aquaculture with native species offers a strategic alternative to enhance food security in the Amazon region. This study evaluated the productive performance of Liposarcus pardalis and Hypostomus hemicochliodon in polyculture with Colossoma macropomum at different stocking densities (1, 3, and 5 fish/m²) for 120 days. Both loricariid species exhibited high survival (>96%) and satisfactory growth, with optimal performance at intermediate densities (3 fish/m²). Polynomial regression estimated optimal stocking densities of 2.45 and 2.42 fish/m² for L. pardalis and H. hemicochliodon, respectively. Polycultures with H. hemicochliodon yielded the highest biomass (22.11 ± 0.09 kg) and best feed conversion efficiency (FCE = 1.4), outperforming those with L. pardalis (19.36 ± 0.19 kg; FCE = 1.9). Although monoculture data for C. macropomum were not included, comparisons with published studies indicate that polyculture did not compromise its growth. The integration of native benthic, iliophagous species improves resource use and nutrient recycling, providing a sustainable strategy to enhance productivity and environmental efficiency in tropical aquaculture systems. Full article

Sustainable aquaculture is increasingly vital to meet global protein demands while ensuring fish product safety and environmental stewardship from a One Health perspective. This review addresses fish hygiene as a comprehensive, multi-stage challenge encompassing water quality management, pathogen control, antimicrobial stewardship, feeding practices, humane slaughter, post-harvest handling, and monitoring systems. We examined current practices and technologies that promote hygienic standards and reduce contamination risks across production cycles. The integration of biosecurity measures and alternative health-promoting agents contributes to disease prevention and reduces reliance on antimicrobials. Responsible drug administration aligned with regulatory frameworks minimizes residues and antimicrobial resistance. Feeding strategies incorporating sustainable and safe ingredients further support fish health and product quality. Critical control points during slaughter and post-harvest processing ensure microbial safety and prolong shelf life. Advanced monitoring and traceability systems enable real-time oversight and enhance food safety assurance. Finally, certification programs and robust regulatory policies are essential to standardize practices and facilitate access to international markets. Collectively, these strategies foster sustainable aquaculture that safeguards public health, maintains ecological integrity, and supports economic viability. This holistic approach positions fish hygiene not as a final quality check, but as an integral, continuously managed component of responsible aquaculture production. Full article

The pursuit of sustainable animal protein sources is critical in light of the environmental, social, and economic challenges associated with conventional livestock production. Although meat, including organic production, remains a valuable source of high-quality protein, diversification is essential to sustainably meet future demand. This review summarizes current knowledge on alternative animal protein sources, with a particular focus on insects and cultured meat in Europe. Insects demonstrate high feed conversion efficiency, require minimal land and water resources, and provide essential amino acids, lipids, and micronutrients, while contributing to circular economy models. Cultured meat presents potential advantages for environmental sustainability and animal welfare; however, its large-scale application depends on technological advances, cost reduction, and supportive regulation. Consumer acceptance remains a challenge influenced by cultural heritage, food neophobia, and product presentation. Policy frameworks, including the European Green Deal and the Farm to Fork Strategy, seek to foster innovation and sustainable food systems. Future perspectives emphasize that conventional and organic meat, insect-based protein, and cultured meat should be regarded as complementary solutions for a balanced and resilient protein supply in Europe. Full article

Aflatoxins (AFs) are toxic metabolites produced by Aspergillus flavus (A. flavus) and Aspergillus parasiticus (A. parasiticus) that contaminate food and feed, posing serious health risks to humans and animals. Consumption of aflatoxin-contaminated foods can cause aflatoxicosis, a serious condition characterised by acute or chronic toxicity. Due to their prevalence, especially in humid regions such as sub-Saharan Africa, proper management is essential for food safety and public health. While traditional methods for controlling aflatoxins can be effective, they are often costly and may introduce harmful chemicals into food, posing risks to the environment and human health. This review paper extensively analyses the toxin binders used to mitigate aflatoxin contamination, discussing their mechanisms of action and effectiveness. It also explores the transition from traditional aflatoxin management strategies to greener alternatives, with a focus on the emerging field of green nanotechnology. Additionally, this paper examines the biosynthesis of nanoparticles (NPs) using metal salt solutions and plant extracts, and their efficacy as inhibitors of aflatoxin-producing fungi and their toxins, demonstrating high effectiveness with minimal toxicity to human health and the environment. Furthermore, the article explores the integration of green nanotechnology into sustainable aflatoxin management and discusses future research directions for developing even more potent interventions through nano-encapsulation with β-cyclodextrin (β-CD). Full article

The management of chronic rumination syndrome in professionally cared-for animals requires a comprehensive and individualized approach. In this case study, a multimodal approach incorporating pharmacological treatment, feeding modifications, and behavioral management was applied to a California sea lion (Zalophus californianus) with rumination syndrome, megaesophagus, and hypothyroidism. Behavioral observations were collected through video recordings both prior to and post-intervention. Interventions included oral naltrexone and contingent reinforcement post-feed to provide alternative enrichment activities after feeding sessions to reduce rumination syndrome, as well as levothyroxine for hypothyroidism. Additionally, dietary modifications involved offering smaller food portions, spreading meals across longer time periods, and feeding in a more upright position to facilitate esophageal passage. Results showed a reduction in the frequency of rumination syndrome, with no visible regurgitated material observed five months post-intervention. The sea lion demonstrated improved engagement in training sessions, voluntary husbandry tasks, and open-water activities, as well as improved interactions with conspecifics. The combination of pharmacological, dietary, and behavioral strategies reduced rumination behavior and improved the animal’s overall quality of life, reinforcing the value of individualized care strategies and multimodal treatment plans in addressing complex medical and behavioral comorbidities. These findings show the importance of individualized, multimodal care plans in managing complex behavioral and medical conditions, and they contribute to advancing animal-welfare practices across species. Full article

This review examines the diverse ways in which probiotics, defined as live microorganisms that provide health benefits to the host when administered in adequate amounts, contribute to animal health and welfare across both livestock and companion species. By modulating gut microbiota, enhancing immune responses, and suppressing harmful pathogens, probiotics represent an effective strategy for disease prevention and performance improvement without reliance on antibiotics. In livestock production, these beneficial microbes have been shown to optimize feed utilization, support growth, and reduce methane emissions, thereby contributing to more sustainable farming practices. Their role extends beyond productivity, as probiotics also help mitigate antimicrobial resistance (AMR) by offering natural alternatives to conventional treatments. In aquaculture, they further promote environmental sustainability by improving water quality and reducing pathogen loads. For companion animals such as dogs and cats, probiotics are increasingly recognized for their ability to support gastrointestinal balance, alleviate stress through gut–brain axis interactions, and aid in the management of common conditions including diarrhea, food sensitivities, and allergies. The integration of probiotics into veterinary practice thus reflects a growing emphasis on holistic and preventive approaches to animal health. Despite these advances, several challenges remain, including variability in strain-specific efficacy, regulatory limitations, and cost-effectiveness in large-scale applications. Emerging research into precision probiotics, host–microbiome interactions, and innovative delivery methods offers promising avenues to overcome these barriers. As such, probiotics can be regarded not only as functional supplements but also as transformative tools that intersect animal health, productivity, and sustainability. Full article

Shrimp farming is one of the fastest-growing food-producing sectors worldwide. However, its rapid expansion has raised concerns about sustainability, particularly regarding the heavy reliance on antibiotics and synthetic additives. Mango leaf powder (MLP), a potential natural alternative to synthetic additives and antibiotics, was evaluated as a dietary supplement in the aquaculture of Litopenaeus vannamei. This study aimed to assess the effects of MLP at 4% and 8% inclusion levels on shrimp growth, antioxidant status, digestive enzyme activities, and hepatopancreatic structure. A total of 540 shrimp were fed experimental diets for 42 days. Both MLP treatments significantly promoted weight gain and specific growth rate. They also enhanced antioxidant enzyme activity rates, such as superoxide dismutase, peroxidase, and catalase, and total antioxidant capacity, while decreasing malondialdehyde levels. The 8% MLP group also showed significantly increased digestive enzyme activities (amylase, trypsin, and lipase) compared to that in the control. These findings demonstrate the potential of MLP as a suitable feed additive that facilitates growth, antioxidant capacity, and digestive ability in shrimp. Importantly, this study reveals the potential of mango leaf powder as a novel feed additive for L. vannamei. Full article

Interest in more sustainable diets for the global population of 528 million companion dogs is steadily increasing, encompassing nutritionally sound cultivated meat, vegan, and microbial protein-based dog foods. Factors driving these alternative dog foods include lower impacts on the environment, fewer welfare problems related to intensively farmed animals and wild-caught fish, and potentially superior canine health outcomes, relative to conventional meat-based dog food. Through a questionnaire with 2639 responses, this study aimed to gain insights into dog guardians’ current feeding patterns and dog food purchasing determinants, acceptance of more sustainable dog diets, and sources of information used for decisions about dog diets. Key results included that 84% (2188/2596) of respondents currently fed either conventional or raw meat-based dog food. More than 43% (936/2169) of this group of respondents who answered found at least one of the more sustainable alternative dog foods acceptable, with purchases of these alternatives hinging most commonly upon the nutritional soundness of the products. Cultivated meat-based dog food was the most popular alternative (selected by 24%, 529/2169), followed by vegetarian (17%, 359/2169), insect-based (16%, 336/2169), and vegan (13%, 290/2169) dog food. The top three information sources used to make decisions regarding dog diets were labels/packaging (selected by 42% of all respondents, 1080/2596), scientific articles/books (38%, 989/2596), and business webpages (35%, 900/2596). Numerous human and dog demographic variables had impacts on current diets, acceptance of alternative diets, and information sources used. Notably, human diet and dog diet were the factors most commonly associated with current and potential purchasing decisions, as well as with information sources used. For instance, greater reductions by guardians in the consumption of animals were associated with greater acceptance of more sustainable dog diets. It should be noted that, due to the reliance on convenience sampling and the overrepresentation of respondents from the UK, of female guardians, of respondents with higher education, and of vegan guardians, the reported relative frequencies of subgroups were not fully representative of the global dog guardian population. Association estimates were based on regression analyses to minimize any resultant bias effects. Full article

There is increasing awareness about the adverse environmental and ‘food’ animal welfare impacts associated with the production of meat-based pet food. However, little is known about cat guardians’ acceptance of more sustainable food choices for the global population of approximately 476 million pet cats. By surveying 1380 cat guardians, this study explored feeding patterns used by guardians, determinants of their cat food choices, and their acceptance levels of more sustainable cat food alternatives. The sources of information used by cat guardians to obtain information about the cat diets they chose were also investigated. Key results included: (1) 51% (620/1211) of cat guardians currently feeding meat-based cat food (raw or conventional) considered at least one or more sustainable alternatives to be acceptable, with cultivated meat-based cat food being the most popular alternative, followed by nutritionally sound vegan cat food; (2) the top five characteristics alternative diets needed to offer to be considered viable were good health outcomes, nutritional soundness, palatability, quality, and environmental sustainability; (3) diet types consumed by cat guardians and their cats were strongly associated; and (4) labels/packaging and veterinarians were the information sources most used, although veterinary staff may have been less trusted as reliable sources of dietary advice by guardians feeding unconventional diets. It should be noted that, due to the reliance on convenience sampling and the overrepresentation of respondents from the UK, of female guardians, of respondents with higher education and of vegan guardians, the reported relative frequencies of subgroups were not fully representative of the global cat guardian population. Association estimates were based on regression analyses to minimize any resultant bias effects. Full article

α-Linolenic acid (ALA) is an important essential omega-3 (ω-3) polyunsaturated fatty acid for the maintenance of human health. Although ALA has traditionally been obtained from plant sources, microbial fermentation has emerged as a promising alternative for its sustainable and cost-effective production. However, most of the present approaches rely on genetically modified organisms, which present regulatory and consumer-acceptance concerns. In this study, we aimed to develop a high-ALA-producing strain of Aspergillus oryzae, a Generally Recognized As Safe (GRAS) microorganism widely used in food production in Japan, through self-cloning, a form of genetic engineering that utilizes only the host’s own DNA. To achieve this, an endogenous ω-3 desaturase gene (fad3), which catalyzes the conversion of linoleic acid to ALA, was identified via BLASTP analysis. Subsequently, a multicopy A. oryzae strain (Aofad3-MC) overexpressing fad3 was constructed. This strain increased ALA production, with ALA comprising 30.7% of the total lipids. Furthermore, k-mer analysis confirmed the absence of foreign vector sequences, verifying that Aofad3-MC was constructed through self-cloning. In addition to the identification of the A. oryzae ω-3 desaturase gene, this study provides a microbial platform for the sustainable production of ALA, with potential applications across the food, feed, and related industries. Full article

The agro-industrial residue known as hemp cake, derived from non-psychoactive Cannabis sativa L., represents a sustainable alternative for the development of protein-rich ingredients. In Ecuador, particularly in Bolívar Province, this by-product has been underutilized. However, similar challenges in the valorization of hemp residues have also been reported in other regions, where they are often discarded or used as low-value animal feed. These issues are not exclusive to Bolívar, and since protein stability depends primarily on drying and storage rather than geographic relocation, the valorization strategies proposed in this study can be extrapolated to other production zones. Protein concentrates were extracted from freeze-dried flower cake (TL, freeze-dried hemp cake) and oven-dried flower cake (TS, oven-dried hemp cake) using isoelectric precipitation, yielding protein concentrates from freeze-dried cake (CPL) and oven-dried cake (CPS). Protein content was determined using the Dumas combustion method, the Bradford dye-binding method, and the bicinchoninic acid (BCA) method. Functional properties such as solubility, water absorption, oil absorption, foaming capacity, and foam stability were evaluated, together with total phenolic and flavonoid content and in vitro antioxidant and anti-inflammatory activity. Results demonstrated high protein values (up to 90.42%), remarkable functional properties, and strong bioactive potential, supporting hemp cake concentrates as sustainable alternatives for food, nutraceutical, and pharmaceutical applications Full article

Egg parasitoids, such as Telenomus remus (Hymenoptera: Scelionidae), face significant challenges after release, as their pupae are exposed to various mortality factors that reduce the efficiency of biological control programs. Therefore, this study aimed to evaluate a honey-solid diet that can feed adults still inside the capsules without sticking the wasps on its surface, enabling parasitoid storage and later field release. Three independent bioassays were performed, each with 20 completely randomized replications. The first bioassay evaluated the acceptance of a solid feed—honey soaked in cotton thread—compared to the traditional form—honey droplets. In the second bioassay, the storage periods after emergence of adults in capsules with honey-solid food were analyzed at 2, 4, 6, and 8 days post-emergence, and the third bioassay studied the efficacy of different release densities of fed adults under field conditions. Parasitoids fed on the honey-solid diet exhibited a 13.3% reduction in parasitism compared to honey droplets. However, the sticky, viscous nature of honey can lead to parasitoids becoming glued, potentially leading to their death. T. remus feeding on the honey-solid diet resulted in low mortality inside the capsules, living up to six days with only 22.2% reduction in parasitism capacity, making it a viable alternative to release and transport fed adult parasitoids, with an increase of around 30% in the released density of parasitoids compared with the parasitoids fed on honey droplets. This flexibility of releasing T. remus up to six days after emergence provided valuable knowledge to establish T. remus as a biocontrol agent. Furthermore, the highest tested parasitoid density of 20,000 parasitoids per hectare obtained the highest parasitism of Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. However, future studies are still required with higher releasing densities and less expensive methods of mass rearing the parasitoid for those higher densities to be economically viable. Full article

In recent years, insects have emerged as a nutritious and eco-sustainable alternative food source, with the house cricket (Acheta domesticus, AD) recently authorized by the European Commission as a novel food. However, the presence of harmful substances in insects poses potential health risks. This study investigated the content of potentially toxic elements (PTEs) such as cadmium (Cd), arsenic (As), lead (Pb), mercury (Hg), nickel (Ni), chromium (Cr), and aluminium (Al) in Acheta domesticus fed diets enriched with graded levels of the red seaweed Palmaria palmata or the brown seaweed Ascophyllum nodosum in two feeding trials. Chemical analyses were carried out by graphite furnace atomic absorption spectrophotometry for all elements except Hg, which was analyzed by thermal decomposition amalgamation atomic absorption spectrometry. The results showed that PTE content in the diets was below the legal limits for feed. The PTEs in AD ranged (mg kg⁻¹ dry matter) as follows: Cd (0.069 ± 0.005–0.127 ± 0.002), As (0.08 ± 0.01–0.36 ± 0.03), Pb (0.05 ± 0.01–0.12 ± 0.01), Hg (0.0065 ± 0.0002–0.0141 ± 0.0010), Ni (0.64 ± 0.06–1.20 ± 0.10), Cr (0.16 ± 0.02–0.58 ± 0.01), and Al (17 ± 2–61 ± 1). AD bioaccumulated As and Hg; however, the PTE levels remained below European Union food safety limits. The absence of non-carcinogenic risk for consumers suggests that AD fed seaweed-enriched diets are a safe, healthy, and low-chemical risk food for humans. Full article