Search Results (24)

Microalgae, with their unparalleled capabilities for sunlight-driven growth, CO₂ fixation, and synthesis of diverse high-value compounds, represent sustainable cell factories for a circular bioeconomy. However, industrial deployment has been hindered by biological constraints and the inadequacy of conventional genetic tools. The advent of CRISPR-Cas systems initially provided precise gene editing via targeted DNA cleavage. This review argues that the true transformative potential lies in moving decisively beyond cutting to harness CRISPR as a versatile synthetic biology “Swiss Army Knife”. We synthesize the rapid evolution of CRISPR-derived tools—including transcriptional modulators (CRISPRa/i), epigenome editors, base/prime editors, multiplexed systems, and biosensor-integrated logic gates—and their revolutionary applications in microalgal engineering. These tools enable tunable gene expression, stable epigenetic reprogramming, DSB-free nucleotide-level precision editing, coordinated rewiring of complex metabolic networks, and dynamic, autonomous control in response to environmental cues. We critically evaluate their deployment to enhance photosynthesis, boost lipid/biofuel production, engineer high-value compound pathways (carotenoids, PUFAs, proteins), improve stress resilience, and optimize carbon utilization. Persistent challenges—species-specific tool optimization, delivery efficiency, genetic stability, scalability, and biosafety—are analyzed, alongside emerging solutions and future directions integrating AI, automation, and multi-omics. The strategic integration of this CRISPR toolkit unlocks the potential to engineer robust, high-productivity microalgal cell factories, finally realizing their promise as sustainable platforms for next-generation biomanufacturing. Full article

This article analyzes the significance of the ruins and remnants of the Soviet Army in Borne Sulinowo, a former secret Soviet military base in Western Pomerania (Poland), as a source of inspiration for performance artists. This study draws from a variety of theoretical frameworks, including performance art theory, new materialism, and the thing theory. Additionally, it draws from the ideas of Carl Lavery, Richard Gough, Ann Laura Stoler, and Georg Simmel. This text delves into the notion that the transient character of performance art mirrors the fleeting nature of power, particularly in the context of the dissolution of the Soviet regime. Following the Polish reacquisition of the site in the early 1990s, artists such as Władysław Kaźmierczak and Brian Connolly transformed found objects and the decaying environment into performance art. This article analyzes performances such as Kaźmierczak’s кoнец фильма (The End of the Movie) and Connolly’s Frieze Frame. It discusses how these works captured the emotional and intellectual responses to the remnants of military occupation. The performances demonstrate the interplay between decay, memory, and historical consciousness, employing the ruins as a medium for reflecting on the collapse of Soviet influence in Poland and the shifting geopolitical landscape. Full article

The present research attempted to investigate the relationship between leadership styles and job satisfaction for civilian personnel of the Ministry of Defense. The research was carried out during the months of May and June in the year 2022. The Multifactor Leadership Questionnaire (M.L.Q.-5X) and the Employee Satisfaction Inventory (ESI) were given via the Internet in digital form to 127 participating technical employees and civilian personnel who served in this specific period in various services of the Ministry of National Defense of Greece. The survey data were statistically analyzed using SPSS program v.22. The results of the dimensional correlations between leadership styles and job satisfaction showed that idealized influence (both attributes and behavior), inspirational motivation, intellectual stimulation, individual consideration, and contingent rewards were positively related to overall employee satisfaction. In contrast, management by exception (active) was not correlated with overall satisfaction. Furthermore, management by exception (passive) and laissez-faire leadership were negatively correlated with overall job satisfaction. Accordingly, the statistical findings of the dimensional correlations concerning the relationship between the three dimensions of leadership outcomes and job satisfaction showed that extra effort, effectiveness, and satisfaction with leadership were positively related to overall satisfaction. Higher effect sizes were reported for the correlation between the contingent reward dimension of process leadership and employee satisfaction, indicating that processing leadership has a stronger effect on the satisfaction of the technical employees of the Ministry of National Defense compared to transformational leadership dimensions. We assume that this result is due to the special characteristics of the Ministry of National Defense workplace. Full article

In recent decades, research on Haredi society has expanded significantly, emphasizing its distinctive characteristics in contrast with the broader Israeli social fabric. Commonly described as “separatist”, “secessionist”, “isolated”, and “an enclave”, Haredi society is often perceived as segregated from the rest of Israel’s population. However, recent studies suggest that this reclusiveness is a relatively recent development. Until the 1980s, most Haredim coexisted in mixed residential areas with secular, traditional, and national-religious populations. The employment rate among Haredi men was comparable to that of other Israelis, and secular subjects were part of the curriculum in Haredi schools. Moreover, many Haredi men served in the Israeli army and held a positive view of the state, celebrating its accomplishments. The contemporary concept of a “society of learners”, where many married men engage in yeshiva study while their wives serve as the main breadwinners, was once largely unacceptable within the community. Unlike earlier qualitative studies that documented the transformation of Haredi society, this article employs a quantitative approach to detail these changes. Through an analysis of residential patterns, education, military service, political influence, and the rise of the “society of learners”, this study provides a deeper understanding of the forces shaping contemporary Haredi society and explores potential future trajectories. Full article

Type 2 diabetes mellitus (T2DM) is a global health concern, with diabetic neuropathy (DN) being a prevalent complication. Current DN treatments focus on blood glucose control and pain management, which show limited efficacy. This study explored the effects of autologous dendritic cell (DC) administration on improving DN symptoms. A quasi-experimental clinical trial was conducted on 28 DN patients at Gatot Soebroto Army Hospital. Patients received autologous DC administration, with their Toronto Clinical Neuropathy Score (TCNS), Transforming Growth Factor-β (TGF-β), and Vascular Cell Adhesion Molecule-1 (VCAM-1) levels measured before and at four weeks after treatment. The results show an average TCNS reduction from 8.93 to 7.5 (p < 0.001). TGF-β levels increased slightly from 41.16 ng/mL to 44.18 ng/mL (p > 0.05). VCAM-1 levels increased from 1389.75 ng/mL to 1403.85 ng/mL. Correlation analysis showed that TGF-β levels had a significant negative correlation with the TCNS (r = −0.353; p = 0.033) and VCAM-1 levels (r = −0.521; p = 0.002). Autologous DC administration significantly improves DN. While the changes in TGF-β and VCAM-1 levels were not statistically significant, their trends suggest that there was an anti-inflammatory effect. These findings highlight the potential of autologous DC therapy as a complementary approach to manage DN through inflammation reduction and nerve repair. Full article

Diabetic kidney disease (DKD) significantly increases mortality, with patients facing a fourfold risk of death within ten years. Chronic inflammation, marked by transforming growth factor-β (TGF-β) and intracellular adhesion molecule-1 (ICAM-1) activity, contributes to kidney damage and fibrosis. This study investigates the effect of autologous dendritic cells on inflammation and kidney function, focusing on apparent diffusion coefficient (ADC), TGF-β, and ICAM-1 levels. This quasi-experimental clinical trial involved 22 DKD patients at Gatot Soebroto Army Hospital. Patients received autologous dendritic cell injections. Baseline and post-intervention magnetic resonance imaging (MRI) scans measured ADC values, and ICAM-1 and TGF-β levels were analyzed. Post intervention, the median ADC decreased from 1.75 mm²/s to 1.64 mm²/s (p = 0.223). ICAM-1 levels increased significantly in females (p = 0.04) but not in males (p = 0.35). No significant changes were found in TGF-β levels (p = 0.506). ADC changes were statistically insignificant and did not correlate with CKD severity. ICAM-1 increases in females correlated with improved creatinine levels, suggesting kidney function improvement. Autologous dendritic cell therapy revealed potential gender-specific immune responses but showed limited overall biomarker improvements. Further studies are required to validate its therapeutic value. Full article

The Topographic Chart of the Kingdom of Poland (pol. Topograficzna Karta Królestwa Polskiego, commonly referred to as ‘the Quartermaster’s Map’, hereinafter: TKKP) is the first Polish modern topographic map of Poland (1:126,000, 1843). Cartographic historians acclaim its conception by the General Quartermaster of the Polish Army, noting its editorial principles and technical execution as exemplars of the early 19th-century cartographic standards. Today, it stands as a national heritage relic, furnishing invaluable insights into the former Polish Kingdom’s topography. Although extensively utilised in geographical and historical inquiries, the TKKP has yet to undergo a comprehensive geomatic investigation and publication as spatial data services. Primarily, this delay stems from the challenges of mosaicking and georeferencing its 60 constituent sheets, owing to the uncertain mathematical framework and irregular sheet cuts. In 2023, the authors embarked on rectifying this by creating a unified TKKP mosaic and georeferencing the map to contemporary reference data benchmarks. This endeavour involved scrutinising the map’s mathematical accuracy and verifying prior findings. The resultant product is accessible via the ‘Maps with the Past’ platform, developed by the Institute of History of the Polish Academy of Sciences The dissemination of raster data services adhering to OGC standards such as WMTS (Web Map Tile Service), ECW (Enhanced Compression Wavelet), and COG (Cloud Optimized GeoTIFF) facilitates the swift and seamless integration of the generated data into web and GIS tools. The digital edition of the TKKP emerges as a pivotal resource for investigations spanning natural and anthropogenic environmental transformations, sustainable development, and cultural heritage studies. Full article

The purpose of this study was to examine competing hypotheses (positive vs. negative) on how organizational members’ familiarity with multiple stakeholders differentially relates to members’ social identity and perception of leadership styles grounded in relational and emotional factors. Specifically, we developed and tested a conceptual model wherein employees’ familiarity with leaders, colleagues, and externals plays a differential role in predicting the extent to which they identify with their workgroup (i.e., group member prototypicality—GMP) and perceive transformational, authentic, leader–member exchange and servant leadership styles. Moreover, we examined the moderating effect of combat experience. We tested this nomological network using structural equation modeling and invariance analyses on a sample of 435 military personnel from the Italian Army (228 combat, 207 non-combat). Results indicated an invariant pattern of relationships among variables for combat and non-combat sub-samples. Specifically, familiarity with leaders positively predicted all leadership styles and GMP. Familiarity with colleagues positively predicted only GMP, whereas familiarity with externals did not predict GMP or leadership factors. Moreover, post hoc quadratic regressions showed a curvilinear inverted-U-shaped relationship between familiarity with colleagues and GMP. Militaries with low or high levels of familiarity with colleagues reported lower levels of GMP compared to militaries with moderate levels of familiarity with colleagues. Hence, at very high levels of familiarity with colleagues, GMP begins to decrease. Theoretical and practical implications of results are discussed in light of the increasing relevance of relational and emotional factors for military leadership, and the current pandemic and geopolitical turmoil. Full article

One of the most important areas of tension for diaconia in the Western European, German-speaking context is the demands of an interreligious and plural society. The social challenges to churches with their congregations and parishes, diaconal organizations, and the state with its social institutions are complex. This article deals with religious layers of social transformations that shape helping actions. It focuses on innovative projects in the field of spiritual care and diaconia that created new spaces of diaconal practice in the last ten years in the Canton of Zurich and Switzerland. Specifically, the process of accrediting the first imam at the University Hospital in Zurich, the employment of chaplains of Muslim and Jewish faith in the Swiss Army, and the training of Muslim chaplains in the Canton of Zurich are presented. On the one hand, the aim is to adequately define the relationship between diaconia and spiritual care in a pluralistic society. On the other hand, interreligious cooperation is analyzed as a process of intercultural communication and transcultural practice. From this, theses for prospective, innovative, and well-founded interreligious cooperation in diaconia can be gained, which can be further developed and discussed in other European and global contexts. Full article

Background: Military logistics has been present from the moment a soldier was created, and its evolution has been influenced by the technology used. Indeed, it has been observed over time that every military revolution was intrinsically caused by a military logistics revolution, directly promoted by the technology applied to the manner of making war. Few papers have been written about military logistics in relation to the development of war itself. Its application was conceptualized in two dimensions: as an art and as a science, interdependent on decision-making. With the evolution of human beings, the way of making war also evolved and therefore a transformation was generated. This makes it necessary to deepen the application of mathematical modeling, statistics, and new technologies in military logistics, promoting the application of concepts based on technology to improve the effectiveness of armies. The aim of this paper is to review the evolution of military logistics. Methods: A systematic approach to the literature review is followed. The backgrounds of the military logistics thought, key authors, trends and a new definition of military logistics is presented, among others. Results: The findings allow us to identify a set of 21 definitions of military logistics since 1792. Full article

This study aims to explore the connection between religious and military spheres in Constantinian propaganda. The extensive use of propaganda and the notorious public discourse which involves the dynamics of power during Late Antiquity show how religion and the military played a key role. This principle reaches a singular meaning in the case of emperor Constantine I. To this extent, this paper considers several kinds of sources, which include legal, literary, and numismatic, among others. An analysis of the political uses of imperial constitutions by the emperor (especially CTh 7.20.2) can be of particular interest in order to address the ideas of self-representation and the politics of legitimation. Ultimately, the paper highlights the importance of imperial propaganda in Later Roman society, as well as the transformations in Constantine’s public discourse, where the connection between army and religion shows an evolution from the previous ways of understanding imperial power and where the bond of the ruler with a supreme divinity is a central issue. Full article

The transformations that occurred in the Ottoman Empire in the eighteenth century, summarized by one author as more army, more taxes, more bureaucracy, and more state intrusion in the Ottoman provinces, radically changed provincial life in the Ottoman domains. Growing tax and manpower demands not only increased socio-economic pressure on the provinces but also redefined the sultan’s relationship with local authorities. Accompanied by the increasingly frequent stationing of the Janissary corps in the Ottoman provinces, especially in the seventeenth century, the Ottoman cities and towns saw new elite configurations and new types of power struggles and came under greater economic pressure. The rising number of registered Janissaries changed the internal dynamics of the towns, shaped local politics, and created new struggles for power in the cities where corps regiments were stationed, pushing the Janissaries into local politics, whether as rivals or allies of the local elite. As elsewhere, the southern Anatolian town of Adana witnessed such changes in its social structure, local politics, and relations with the imperial authority. Although similarities are to be seen with the eighteenth century provincial power struggles in the Anatolian and Arabian cities of Gaziantep and Aleppo in terms of intense factional strife and the active involvement of the Janissaries and their pretenders in local politics, the power struggle in Adana was between several Janissary officers, one of whom subsequently managed to become the urban notable (ayan) of the town. Full article

A new phase-based filter (called improved analytical signal (IAS)) is introduced to detect buried unexploded ordnance (UXO) precisely from magnetic fields using the arcsine function of the ratio of the first-order vertical derivative of the analytical signal to the first-order derivatives of the x-, y-, and z-components of the analytical signal. The calculations are computed in the frequency domain and then transformed back into the space domain using the inverse Fourier transform. The filter has been tested on magnetic data collected at a test site with UXO bodies of variable orientation. It was also validated on magnetic data measured at a former army artillery range in Slovakia. The results show that the IAS filter not only revealed better imaging of the UXO bodies compared to the other commonly used filters but also produced a high-resolution image with much less influence of noise. Full article

In this study, the required capabilities and key element technologies of smart underground ammunition storage were analyzed using the Delphi technique. Twenty-four external experts on industry 4.0 smart technology were selected. A total of 18 required capabilities and 32 key element technologies were derived for the construction of smart underground ammunition storage. Smart ammunition storage can be built through the convergence of a range of ICT technologies such as sensors, clouds, big data, precision control, and mobile technologies, along with existing ammunition storages. The combination of these technologies will support human decision making through the use of numerous sensors applied to ammunition storage for collecting data and converting them into big data. In addition, the intelligent information technology introduced in a smart ammunition store will allow soldiers to detect changes in the surrounding environment, which will bring about innovation to an ammunition service. As a result, high-level automation and an intelligent infrastructure can be provided, enabling an improvement in ammunition management capabilities, energy saving, and the establishment of a safe operating environment and a flexible management system. This form of future ammunition storage will be an example solution for major issues in army ammunition services, as well as overcoming challenges such as a reduction in military forces. Full article

Previous studies of human response to whole-body vibration demonstrated nonlinear softening behaviors with increasing vibration magnitudes. Most of these studies were conducted at relatively low vibration magnitudes of less than 3 m/s² root mean square (RMS), and not much knowledge is available to show if this softening behavior exists when humans are exposed to higher vibration magnitudes. In this work, 26 participants were transported in a supine position inside an army medical vehicle on a road that simulated field scenarios and were exposed to input acceleration magnitudes at 0.60, 0.98, 1.32, 3.25, 5.58, and 5.90 m/s² RMS. Motion response data were collected at the head, torso, and pelvis of the participants using inertial sensors. Transmissibility and coherence graphs were used to investigate the type of nonlinearity induced under these transport conditions. Participant responses showed softening behavior when the vibration magnitude increased from 0.60 to 0.98 to 1.32 m/s² RMS. However, this response behavior changed to stiffening when the vibration magnitude increased to 3.25, 5.58, and 5.90 m/s² RMS. In the stiffening range, the transmissibility of the torso transformed from two dominant peaks to a single peak, which may indicate a tonic muscle behavior. The resulting stiffening behaviors may be considered in the design of transport systems subject to rough terrains. Full article