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Keywords = bacterial grouting strategies

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24 pages, 3088 KB  
Article
Comprehensive Evaluation of Biogas Slurry Fertility: A Study Based on the Effects of Biogas Slurry Irrigation on Soil Microorganisms and Enzyme Activities in Winter Wheat Fields
by Dongxue Yin, Jiajun Qin, Baozhong Wang, Dongdong Chen, Zhiguang Dai, Xiaoli Niu, Jie Zhu and Fengshun Zhang
Microorganisms 2025, 13(9), 2054; https://doi.org/10.3390/microorganisms13092054 - 4 Sep 2025
Viewed by 387
Abstract
This study evaluates the impact of using biogas slurry (BS) instead of nitrogen fertilizer (NF) on wheat soil, and aims to provide an optimized fertilization strategy for green wheat production. Five fertilization modes were tested: basal fertilizer only (CK), NF at the full-bearing [...] Read more.
This study evaluates the impact of using biogas slurry (BS) instead of nitrogen fertilizer (NF) on wheat soil, and aims to provide an optimized fertilization strategy for green wheat production. Five fertilization modes were tested: basal fertilizer only (CK), NF at the full-bearing stage (CF), BS at the jointing stage + NF at the grouting period (S1), NF at the jointing stage + BS at the grouting period (S2), and BS at the full-bearing stage (S3). Wheat yield in S3 treatment was not significantly different from CF (9632.57 kg·ha−1), but significantly increased starch content by 23.39% (p < 0.05). Analysis of soil nutrient content showed that S3 treatment elevated ammonium nitrogen (AN) content by 98.30% during the harvest period and maintained the highest urease activity (686.45 μg·g−1·d−1). Microbial community analysis showed that the bacterial Shannon index under S3 treatment reached 7.09, and the abundance of Actinomycetes reached 39.40%. The fungal Simpson index was 0.02, lower than that of other treatments (p < 0.01). A comprehensive evaluation led to the conclusion that a complete replacement of BS with NF synergistically improves soil quick-acting nutrient levels, enhances soil enzyme activities, and sustains high microbial diversity, whilst maintaining wheat yield. Full article
(This article belongs to the Special Issue Soil Environment and Microorganisms)
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21 pages, 6227 KB  
Article
A One-Phase Injection Method with Dual Inhibition for Improving the Strength and Uniformity of MICP
by Yanni Huang, Fengyin Liu and Xiangtong Zhang
Materials 2025, 18(11), 2514; https://doi.org/10.3390/ma18112514 - 27 May 2025
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Abstract
The formation and spatial uniformity of calcium carbonate (CaCO3) are critical for evaluating the effectiveness of microbial-induced calcium carbonate precipitation (MICP) in geotechnical applications. In recent years, the single-phase injection method has emerged as a promising alternative to traditional two-phase processes [...] Read more.
The formation and spatial uniformity of calcium carbonate (CaCO3) are critical for evaluating the effectiveness of microbial-induced calcium carbonate precipitation (MICP) in geotechnical applications. In recent years, the single-phase injection method has emerged as a promising alternative to traditional two-phase processes by addressing the issue of uneven CaCO3 distribution. This study proposes a dual inhibition strategy that delays the mineralization reaction by synergistically lowering pH and temperature, thereby promoting uniform precipitation and enhanced compressive strength in cemented sand columns. A series of experiments, including bacterial growth, aqueous reaction, sand column reinforcement, and microstructural characterization, were conducted. Results show that the minimum pH required for flocculation increases from ~4.5 at 40 °C to ~6.0 at 10 °C. Under dual inhibition, the lag period effectively improved the spatial uniformity of CaCO3 and enabled complete calcium utilization within 24 h. After four treatment cycles, the CaCO3 content at 10 °C increased by 53%, and the unconfined compressive strength reached 2.5 MPa, a 50% improvement over the 40 °C condition. XRD analysis confirmed that calcite was the dominant phase (85–90%), accompanied by minor vaterite. These findings demonstrate the adaptability and efficiency of the dual inhibition method across temperature ranges, providing a cost-effective solution for broader engineering applications. Full article
(This article belongs to the Section Biomaterials)
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