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Keywords = blackcurrant leaf chlorosis associated virus

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14 pages, 6996 KB  
Article
Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections
by Delano James, James Phelan and Daniel Sanderson
Viruses 2018, 10(5), 260; https://doi.org/10.3390/v10050260 - 15 May 2018
Cited by 6 | Viewed by 4721
Abstract
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-terminus of RNA-2 [...] Read more.
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion. Evidence was obtained indicating that there are circular forms of BCLCaV RNA-2 and RNA-3. Full article
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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