Search Results (14,721)

A surface molecularly imprinted ratiometric fluorescent sensor (Eu/CDs@SiO₂@IIPs) was constructed for the selective and visual detection of Cu²⁺. The sensor integrates blue-emitting carbon dots as an internal reference and a custom-designed Eu(III) complex, Eu(MAA)₂(2,9-phen), as both the functional and fluorescent monomer within a surface-imprinted polymer layer, enabling efficient ratiometric fluorescence response. This structural design ensured that all fluorescent monomers were located at the recognition sites, thereby reducing background fluorescence interference and enhancing the accuracy of signal changes. Under optimized conditions, the sensor exhibited a detection limit of 2.79 nM, a wide linear range of 10–100 nM, and a rapid response time of 3.0 min. Moreover, the uncoordinated nitrogen atoms in the phenanthroline ligand improved resistance to interference from competing ions, significantly enhancing selectivity. Practical applicability was validated by spiked recovery tests in deionized and river water, with results showing good agreement with ICP-MS analysis. These findings highlight the potential of Eu/CDs@SiO₂@IIPs as a sensitive, selective, and portable sensing platform for on-site monitoring of Cu²⁺ in complex water environments. Full article

This study focuses on the preparation of poly(HCQM-co-VP) copolymeric nanoparticles (NPs) to enhance the aqueous solubility and bioavailability of the hydrophobic and antitumor molecules HCQ (hydroxychloroquine) and α-TOS (α-tocopheryl succinate). HCQ is covalently incorporated into the polymer backbone, while α-TOS is encapsulated within the nanoparticles by non-covalent interactions. Poly(HCQM-co-VP) was synthesized from a vinyl derivative of HCQ (HCQM) and N-vinylpyrrolidone (VP), with a molar composition of 17% HCQM and 83% VP, providing the optimal hydrophobic/hydrophilic balance for forming, via nanoprecipitation, empty nanoparticles (NPs) with a diameter of 123.6 nm and a zeta potential of −5.8 mV. These nanoparticles effectively encapsulated α-TOS within their hydrophobic core, achieving an encapsulation efficiency (%EE) of 78%. These α-TOS-loaded NPs resulted in smaller diameters and more negative zeta potentials (71 nm, −19.2 mV) compared to the non-loaded NPs. The cytotoxicity of these NPs was evaluated using the AlamarBlue assay on MCF-7 breast cancer cells. The empty NPs showed no toxic effects within the tested concentration range, after 72 h of treatment. In contrast, the α-TOS-loaded NPs, exhibited a pronounced cytotoxic effect on MCF-7 cells with an IC₅₀ value of 100.2 μg·mL⁻¹, thereby demonstrating their potential as controlled drug delivery systems for cancer treatment. These findings contribute to the development of a new HCQ-based polymeric nanocarrier for α-TOS or other hydrophobic drugs for the treatment of cancer and other diseases treatable with these drugs. Full article

The green macroalgae Ulva Linnaeus, 1753, also known as sea lettuce, is one of the most ecologically and economically significant algal genera. Its representatives occur in marine, brackish, and freshwater environments worldwide and show high adaptability, rapid growth, and marked biochemical diversity. These traits support their ecological roles in nutrient cycling, primary productivity, and habitat provision, and they also explain their growing relevance to the blue bioeconomy. This review summarizes current knowledge of Ulva biodiversity, taxonomy, and physiology, and evaluates applications in food, feed, bioremediation, biofuel, pharmaceuticals, and biomaterials. Particular attention is given to molecular approaches that resolve taxonomic difficulties and to biochemical profiles that determine nutritional value and industrial potential. This review also considers risks and limitations. Ulva species can act as hyperaccumulators of heavy metals, microplastics, and organic pollutants, which creates safety concerns for food and feed uses and highlights the necessity of strict monitoring and quality control. Technical and economic barriers restrict large-scale use in energy and material production. By presenting both opportunities and constraints, this review stresses the dual role of Ulva as a promising bioresource and a potential ecological risk. Future research must integrate molecular genetics, physiology, and applied studies to support sustainable utilization and ensure safe contributions of Ulva to biodiversity assessment, environmental management, and bioeconomic development. Full article

In this study, the use of spent coffee waste as a green precursor of polyphenolic compounds, which are subsequently employed as reducing agents for the synthesis of zero-valent iron nanoparticles (nZVI) aimed at the efficient removal of dyes from aqueous systems, has been investigated. The nanoparticles, generated in situ in the presence of controlled amounts of hydrogen peroxide, were applied in the removal of organic dyes—including methylene blue, methyl orange, and orange G—through a heterogeneous Fenton-like catalytic process. The synthesized nZVI were thoroughly characterized by nitrogen adsorption at 77 K, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (XRD). A statistical design of experiments and response surface methodology were employed to evaluate the effect of polyphenol, Fe(III), and H₂O₂ concentrations on dye removal efficiency. Results showed that under optimized conditions, a 100% removal efficiency could be achieved. This work highlights the potential of nZVI synthesized from agro-industrial waste through sustainable routes as an effective solution for water remediation, contributing to circular economy strategies and environmental protection. Full article

The Upper Blue Nile Basin (UBNB), which contributes about 60% to the annual Nile flow, plays a critical role in the Nile water management. However, its complex terrain and climate create significant challenges for accurate regional climate simulations, which are essential for climate impact assessments. This study aims to address the challenges of climate simulation over the UBNB by enhancing the Regional Climate Model system (RegCM5) with its new non-hydrostatic dynamical core (MOLOCH) to simulate precipitation and temperature. The model is driven by ERA5 reanalysis for the period (2000–2009), and two scenarios are simulated using two different schemes of the Planetary Boundary Layer (PBL): Holtslag (Hol) and University of Washington (UW). The two scenarios, noted as (MOLOCH-Hol and MOLOCH-UW), are compared to the previously best-performing hydrostatic configuration. The MOLOCH-UW scenario showed the best precipitation performance relative to observations, with an accepted dry Bias% up to 22%, and a high annual cycle correlation >0.85. However, MOLOCH-Hol showed a very good performance only in the wet season with a wet bias of 4% and moderate correlation of ≈0.6. For temperature, MOLOCH-UW also outperformed, achieving the lowest cold/warm bias range of −2% to +3%, and high correlations of ≈0.9 through the year and the wet season. This study concluded that the MOLOCH-UW is the most reliable configuration for reproducing the climate variability over the UBNB. This developed configuration is a promising tool for the basin’s hydroclimate applications, such as dynamical downscaling of the seasonal forecasts and future climate change scenarios produced by global circulation models. Future improvements could be achieved through convective-permitting simulation at ≤4 km resolution, especially in the application of assessing the land use change impact. Full article

The seafood industry is increasingly adopting intelligent packaging to preserve product quality and improve freshness transparency. This study developed and evaluated a pH-sensitive freshness indicator (FI) for skate sashimi (Zearaja chilensis). This product is consumed at varying stages of fermentation. The FI incorporated bromothymol blue (BTB) and bromocresol purple (BCP) in a polymer matrix. It targeted volatile basic nitrogen (VBN) compounds, with trimethylamine (TMA) as the primary marker. As freshness declined, VBN compounds accumulated in the package headspace and caused a gradual FI color change from yellow to blue through pH variation. ΔE increased from 7.72 on day 2 to 23.52 on day 3. This marked the onset of visible color change and the FI reached full blue by day 7. Headspace solid-phase microextraction (HS-SPME) and gas chromatography–flame ionization detection (GC-FID) quantified monomethylamine (MMA), dimethylamine (DMA) and TMA throughout storage. ΔE correlated strongly with total bacterial count (TBC, r = 0.978), pH (r = 0.901) and TMA (r = 0.888). These results indicate that microbial growth, alkalinity increase and amine production were closely associated with color transitions. The FI reliably tracked freshness loss in skate sashimi. It has potential to enhance consumer transparency and strengthen quality control in the seafood supply chain. Full article

Over the past decade, increasing attention has been given to the impacts of anthropogenic microparticle (AM) pollution on marine ecosystems. This study investigates AM ingestion in three commercially important fish species—Sparus aurata Linnaeus, 1758, Dicentrarchus labrax Linnaeus, 1758, and Boops boops Linnaeus, 1758—collected from both wild and farmed populations in Greek marine and lagoon environments. A total of 60 specimens were sampled from the Messolonghi Lagoon, Rhodes Island, and the Cyclades. AM were detected in 61.7% of the individuals analyzed. The mean number of ingested items per individual was 1.1 ± 1.2 in B. boops, 1.0 ± 1.7 in wild and 2.3 ± 2.1 in farmed S. aurata, and 2.5 ± 3.1 in wild and 3.6 ± 2.2 in farmed D. labrax. Ingestion ranged from 0 to 9 items per fish. No significant correlations were found between fish size and either the number or the size of ingested AM in any species. The ingested AM were primarily classified as fibers and fragments, displaying variability in size and color. Black was the dominant color across all species, followed by red and blue, while yellow was rarely observed. A statistically significant difference in the mean size of AM was recorded between wild and farmed D. labrax, whereas no such difference was observed for S. aurata. Overall, these findings provide new evidence on AM contamination in seafood species and highlight their occurrence in both natural and aquaculture environments of the eastern Mediterranean. Full article

As the direct energy source in organisms, accurate and simple detection of adenosine triphosphate (ATP) is of great significance. Herein, a colorimetric aptasensor for ATP determination was designed by integrating the CRISPR/Cas12a system with an aptamer, and with Prussian blue nanocube and gold nanoparticle co-functionalized MoS₂ (MoS₂-PBNCs-AuNPs) nanozymes. As expected, the introduced CRISPR/Cas12a system and aptamer could efficiently amplify the detection signal and improve the specific recognition ability, respectively. Meanwhile, the catalytic activity of the MoS₂-PBNCs-AuNPs nanozymes can be regulated with the concentration of ATP. The high-affinity binding of ATP to the aptamer competitively inhibited aptamer-crRNA hybridization, causing fewer Cas12 proteins to be activated. As a result, the uncleaved single-stranded DNA (ssDNA) adsorbed onto the surface of nanozymes to effectively enhance their catalytic oxidation capability toward 3,3′,5,5′-tetramethylbenzidine (TMB). According to this phenomenon, this CRISPR-enhanced colorimetric aptasensor can detect down to 0.14 μM ATP with high selectivity, reproducibility, and stability. In addition, acceptable recoveries and low relative standard deviations of the aptasensor for ATP determination suggest that it is promising for application in early detection of clinical-related diseases. Full article

The Blue Economy has emerged as a vital framework for achieving sustainable economic diversification, environmental stewardship, and social resilience, particularly in regions facing ecological pressures such as the Gulf Cooperation Council (GCC). Despite its increasing recognition in national strategies, including Saudi Vision 2030 and the UAE’s Blue Economy Strategy 2031, scholarly research in the GCC remains fragmented and uneven. This study provides the first comprehensive bibliometric and thematic review of Blue Economy research in the region, analyzing publications produced between 2000 and 2025. The analysis reveals four dominant thematic clusters: fisheries and food security, governance and coastal policy, climate resilience and ecosystem restoration, and blue finance and economic diversification. At the same time, it identifies persistent gaps in social equity, gender inclusivity, traditional ecological knowledge, and regional coordination. By situating GCC research within broader global debates, the study underscores both the strengths and limitations of the current knowledge base. The findings contribute to academic debate and policy development by offering a conceptual framework that highlights inclusive governance, innovative financing, and nature-based solutions as key pillars for future research and practice. In doing so, the study provides a roadmap for advancing the Blue Economy agenda in the GCC and beyond. Full article

Aronia melanocarpa is rich in anthocyanins, compounds with significant medicinal and industrial value, making it an attractive species for enhanced production. Compared with fruits or intact plants, callus tissue offers a uniform, controllable in vitro system that is particularly suitable for dissecting regulatory mechanisms under defined environmental conditions. Although light quality is known to influence anthocyanin biosynthesis, its specific regulatory mechanisms in A. melanocarpa remain unclear. In this study, callus tissues were cultured under six light regimes: full-spectrum LED, blue:red (5:1), red:blue (5:1), red:blue:white (1:1:1), red:white (5:1), and pure blue light. Anthocyanin content was quantified using the pH differential method, and the results showed that the blue:red (5:1) treatment produced the highest accumulation, reaching 14.06 mg/100 g. Transcriptome sequencing was then performed to compare the gene expression profiles between calli cultured under blue:red (5:1) light and those maintained in darkness. A total of 10,547 differentially expressed genes (DEGs) were identified, including 6134 upregulated and 4413 downregulated genes. Functional enrichment analysis indicated that these DEGs were mainly involved in anthocyanin biosynthesis and transport. Importantly, key structural genes such as PAL, C4H, 4CL, CHS, ANS, UFGT, and GST were significantly upregulated under blue:red (5:1) light, as further validated by qRT-PCR. Overall, our findings demonstrate that a blue:red (5:1) light ratio enhances anthocyanin accumulation by promoting the expression of biosynthetic and transport-related genes. This study not only provides new transcriptomic insights into the light-mediated regulation of secondary metabolism in A. melanocarpa callus, but also establishes a foundation for optimizing in vitro culture systems for sustainable anthocyanin production. Full article

Membranes were assessed on a bench scale for their performance in methylene blue dye separation. The sawdust, along with Brazilian clay and kaolin, were mixed and compacted by uniaxial pressing and sintered at 650 °C. The membranes were characterized by several techniques, including X-ray diffraction, scanning electron microscopy, porosity, mechanical strength, water uptake, and membrane hydrodynamic permeability. The results demonstrated that the incorporation of sawdust not only altered the pore morphology but also significantly improved water permeation and dye removal efficiency. The ceramic membrane had an average pore diameter of 0.346–0.622 µm and porosities ranging from 40.85 to 42.96%. The membranes were applied to the microfiltration of synthetic effluent containing methylene blue (MB) and, additionally, subjected to investigation of their adsorptive capacity. All membrane variants showed high hydrophilicity (contact angles < 60°) and achieved MB rejection efficiencies higher than 96%, demonstrating their efficiency in treating dye-contaminated effluents. Batch adsorption using ceramic membranes (M0–M3) removed 34.0–41.2% of methylene blue. Adsorption behavior fitted both Langmuir and Freundlich models, indicating mixed mono- and multilayer mechanisms. FTIR confirmed electrostatic interactions, hydrogen bonding, and possible π–π interactions in dye retention. Full article

The dried herb of blue fenugreek is used as a spice in the alpine region for the preparation of traditional bread and cheese. After drying, the herb is stored for a period of six to twelve months. During this time, the herb is expected to undergo changes in the compositions of the major flavor- and odor-determining compounds. To identify eventual biochemical processes, we applied different growing (conventional and sterile) and drying (air- and freeze drying) conditions and subsequently conducted periodical analysis of key aroma constituents (α-keto acids and volatile compounds) by LC-MS and GC-MS. The amount of glyoxylic acid was drastically increased in the air-dried sample, while the freeze-dried sample showed significantly higher amounts of α-keto-glutaric acid and pyruvic acid, respectively. During storage, a decrease in sulfuric compounds and an increase in alkane aldehydes were observed when comparing conventional and sterile samples. However, this increase was even greater for monoterpenes (especially camphor and p-cymene), showing thrice as high amounts after storage. Interestingly, both compounds were only formed significantly during the storage under conventional conditions, indicating that their production is induced/caused by microbial organisms. Full article

This study investigated the correlation between the dispersion stability and photocatalytic efficiency of titanium dioxide (TiO₂) nanoparticles for the development of self-cleaning functional concrete. After pretreatment of P25 TiO₂ with aqueous solutions of polyvinyl alcohol (PVA), polyethylene glycol (PEG), and polyethylene glycol methyl ether (PEGME), dynamic light scattering (DLS) and zeta potential measurements were performed, and as a result, a 0.1 wt% PVA solution was optimal for inhibiting aggregation, with an average hydrodynamic diameter of 1.4 µm and a zeta potential of −11 mV. In methylene blue photolysis, the reaction rate constant (k_app) was 1.71 × 10⁻² min⁻¹ (R² = 0.98), which was improved by 11.4 times compared to the control group, and was about twice as high in the concrete specimen experiment. X-ray diffraction (XRD), scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET) analyses confirmed an anatase-to-rutile ratio of 81:19 particle sizes of 10–30 nm, and a specific surface area of 58.985 m²·g⁻¹. As a result, it is suggested that PVA pretreatment is a practical method to effectively improve the photocatalytic performance of TiO₂-based self-cleaning concrete. Full article

Titanium dioxide (TiO₂), owing to its excellent photocatalytic performance and environmental friendliness, holds great potential in the remediation of water pollution. In this study, we introduce a green and facile strategy to fabricate TiO₂-based hybrid aerogels, in which the peptide FQFQFIFK first self-assembles into peptide nanofibers (PNFs), followed by in situ biomineralization of TiO₂ on the PNFs. The TiO₂-loaded PNFs are then combined with graphene oxide (GO) via π–π interactions and integrated with microcrystalline cellulose (MCC) to construct a stable three-dimensional (3D) porous framework. The resulting GO/MCC/PNFs-TiO₂ aerogels exhibit high porosity, low density, and good mechanical stability. Photocatalytic experiments show that the aerogels efficiently degrade various organic dyes (methylene blue, rhodamine B, crystal violet, and Orange II) and antibiotics (e.g., tetracycline) under visible-light irradiation, achieving final degradation efficiencies higher than 90%. The excellent performance is attributed to the synergistic effect of the ordered interface provided by the PNF template, the stabilization and uniform dispersion facilitated by GO, and the mechanically robust 3D scaffold constructed by MCC. This work provides an efficient and sustainable strategy for designing functional hybrid aerogels and lays a foundation for their application in water treatment and environmental remediation. Full article

Anthropogenic ponds have the potential to shape the post-industrial landscape and mitigate the effects of climate change, particularly in urban heat island-threatened areas. However, decisions regarding their inclusion in blue–green infrastructure networks require balancing costs and benefits while considering potential pollution risks. The objectives of this study are: (i) to develop an efficient decision-making framework based on standard aquatic science tools; (ii) to apply this framework to a specific artificial pond in the Upper Silesian Industrial Region, Poland, in order to optimize actions based on resources, advantages, limitations, and informativeness of the data. Eighteen methods, grouped into five categories, including historical document analyses, hydroacoustic and modeling methods, multiparametric water quality measurements, and ecotoxicological tests, were used. Optimization-focused analysis indicated that investigating historical documents should precede further testing, as it enables decision-makers to select the most effective methods to assess the pond’s value for blue–green infrastructure. In this case, the tests based on metal pollution, bathymetry, and biodiversity appeared sufficient. The presented approach offers a straightforward screening method for assessing reservoirs in post-industrial areas. Full article