Search Results (9,551)

Background/Objectives: Ulcerative colitis (UC) is a type of inflammatory bowel disease characterized by abdominal pain, diarrhea, and bleeding. Polydeoxyribonucleotide (PDRN), an adenosine A_2A receptor (A_2AR) agonist, exhibits anti-inflammatory properties. In the present study, we evaluated the therapeutic effects of PDRN in a dextran sodium sulfate (DSS)-induced murine model of UC. Methods: UC was induced by administering 2% DSS in drinking water for 7 days. One day after DSS administration, mice received intraperitoneal injections of PDRN (8 mg/kg) for 7 days. To investigate the involvement of A_2AR, the selective antagonist 3,7-dimethyl-1-propargylxanthine (DMPX, 8 mg/kg) was co-administered with PDRN. Results: DSS administration induced colonic tissue damage and increased disease activity index (DAI) and histological scores. DSS also elevated pro-inflammatory cytokines while reducing anti-inflammatory cytokine levels. PDRN treatment reduced histological damage, restored body weight, colon weight, and colon length, and decreased DAI scores. Furthermore, PDRN treatment inhibited nuclear factor kappa B (NF-κB) activation through suppression of NF-κB inhibitor-α phosphorylation and was associated with activation of the cAMP/PKA/CREB signaling pathway. PDRN treatment attenuated inflammation and was associated with increased expression of vascular endothelial growth factor (VEGF) in colonic tissues. Given the context-dependent role of VEGF in inflammatory bowel disease, this increase is interpreted as contributing to mucosal repair rather than exacerbating inflammation. Co-administration of DMPX abolished these effects, suggesting the involvement of A_2AR-dependent signaling pathways. Conclusions: PDRN attenuated colonic inflammation and improved disease outcomes in DSS-induced UC, potentially through modulation of the PKA/CREB/NF-κB signaling pathway and VEGF-mediated tissue repair mechanisms. Full article

Aim: This study assessed the effects of a six-week educational intervention using an adapted “Snakes and Ladders” board game on hydration knowledge, behavior, and status among Tunisian elementary school children during physical education (PE) lessons. Method: A randomized controlled trial involving 207 children was conducted, with participants assigned to either the educational group (EG, n = 99) or the control group (CG, n = 108). The EG participated in six weekly 30-min board game sessions, while the CG continued regular activities. Knowledge of hydration was assessed using a validated questionnaire. Hydration status was monitored indirectly by the percentage change in body mass from pre- to post-PE session. Perceived thirst was evaluated using a 9-point scale, and hydration behavior was evaluated based on water consumption during PE lessons. Results: Following the intervention, the EG demonstrated a significant improvement in overall hydration knowledge (ΔMean = +0.30 ± 0.11 vs. −0.05 ± 0.08 in CG; p < 0.001). Water intake during PE sessions increased progressively from week 2 (83.6 ± 127.2 mL) to week 6 (311.2 ± 204 mL) in the EG, whereas it remained unchanged in the CG (p < 0.001). Body mass loss after PE sessions decreased significantly in the EG (−0.03 kg) compared with the CG (−0.16 kg; p < 0.001), and perceived thirst before and after PE sessions was markedly lower (Cohen’s d = 0.75–1.32). Conclusions. The six-week board-game intervention appeared effective in increasing hydration knowledge, promoting healthier drinking behaviors during PE, and improving indirect indicators of hydration status. These findings provide preliminary evidence for the feasibility and educational value of a gamified, low-cost approach to hydration promotion in schools. Further research should examine long-term retention, include objective hydration biomarkers, and evaluate applicability across diverse school settings and environmental conditions. Trial Registration: This study is registered with Pan African Clinical Trial Registry (PACTR) under the unique identifier (PACTR202510771129829). Full article

Contamination of water bodies by emulsified gasoline hydrocarbons, particularly BTEX compounds (benzene, toluene, ethylbenzene, and xylenes), represents a critical environmental challenge due to their toxicity and resistance to conventional treatment methods. In this study, carbonized biosorbents derived from rice husk (CRH) and walnut shell (CWS) were developed for efficient removal of emulsified gasoline from water. The materials were prepared via carbonization under CO₂ atmosphere (300–800 °C), enabling simultaneous carbonization and activation. Structural and surface properties were characterized using Brunauer–Emmett–Teller (BET) analysis, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and X-ray fluorescence spectroscopy (XRF). The results demonstrated a strong dependence of adsorption performance on carbonization temperature, with maximum removal efficiencies of 90.2% (CRH-600) and 96.5% (CWS-700). The superior performance of CWS-700 was associated with its highly developed hierarchical pore structure (up to 670 m² g⁻¹), increased carbon content, and enhanced hydrophobicity. Kinetic studies revealed pseudo-second-order behavior, with equilibrium achieved within 25–30 min at near-neutral pH. Gas chromatographic analysis confirmed the complete removal of BTEX and light hydrocarbons (C1–C9) using CWS-700, highlighting its high selectivity toward aromatic compounds. The adsorption mechanism was attributed to the synergistic effect of micropore filling, hydrophobic interactions, and π-π interactions with aromatic hydrocarbons. The obtained results demonstrate that biomass-derived carbon materials, particularly walnut shell-based sorbents, are promising low-cost candidates for the treatment of complex water systems contaminated with emulsified petroleum hydrocarbons. Full article

The purpose of this article is to evaluate the quality of a reservoir’s surface water through the application of fuzzy-logic methodologies. Specifically, fuzzy-clustering (Fuzzy C-Means) and fuzzy-regression techniques are employed to classify and model the physicochemical and microbiological parameters of the water. The analyzed dataset indicates a particularly close and interesting relationship between the fuzzy-regression results and the fuzzy c-means-clustering results. Hence, the centers of the clusters lie very close to the central value of the produced fuzzy-regression band. This behavior appears when four clusters are used, while the evaluation (performed using the Partition Coefficient (VPC) and the Partition Entropy (VPE) indices) also indicates that four clusters is the most appropriate choice. Full article

Lake Taihu is a vital source of surface water for the Yangtze River Delta region, so effective monitoring of its water quality is essential for protecting the water source. However, most existing studies on unmanned aerial vehicle (UAV)-based water quality remote sensing have focused on single large rivers or lakes, primarily employing validation methods involving randomly selected samples. This makes it difficult to assess the generalisability of the models to unfamiliar watercourses. This study focuses on 13 inflow rivers on Xishan Island, a central island in Lake Taihu, which are characterized by short flow paths, independent catchment areas, and varying land use influences. Using a UAV multispectral remote sensing platform, we have designed a water quality monitoring and assessment framework tailored to multi-river systems with small sample sizes. First, various water body indices were developed and analysed for correlation with measured water quality parameters. Then, machine learning algorithms such as Backpropagation (BP) neural networks, Random Forest, XGBoost, Convolutional Neural Networks (CNN) and Support Vector Machines (SVM) were selected to construct retrieval models. For accuracy evaluation, a spatial independent validation strategy was employed whereby one sample was forcibly set aside from each river to constitute the validation set. Using this method, we generated spatial distribution maps of water quality parameters for the inflow rivers and evaluated the influencing factors of spatial variation in water quality by area, taking into account water body functional types and ecological characteristics. The experimental results indicate that under the conditions of spatial independent validation strategy, the SVM model achieved the highest retrieval accuracy for dissolved oxygen (R² = 0.892, RMSE = 0.414 mg/L and MRE = 0.057), whereas the XGBoost model achieved the highest retrieval accuracy for turbidity (R² = 0.853, RMSE = 0.632NTU and MRE = 0.065). The spatial pattern of water quality exhibited a pronounced gradient: dissolved oxygen (DO) concentrations followed the order of aquaculture area rivers > agricultural area rivers > urban area rivers, while turbidity displayed the opposite trend, reflecting that surrounding land use types, phytoplankton density, and human activity intensity are the dominant factors driving the spatial differentiation of river water quality on Xishan Island in spring. The full-chain technical framework of “multi-river synchronous retrieval—spatially independent validation strategy—area mechanistic assessment” proposed in this study provides a replicable evaluation paradigm for rapid water quality monitoring of Lake Taihu islands and similar watersheds, and holds significant implications for the construction of the Lake Taihu Eco-Island and the protection of the water environment. Full article

Submarine landslides pose severe marine geological hazards. Their movement and deposition behaviors can seriously threaten marine engineering stability and coastal safety. The propagation characteristics of landslide-generated tsunamis are therefore critical for hazard assessment. Physical model experiments provide an effective approach for investigating the underlying mechanisms of tsunami generation and propagation. To investigate the complete process from landslide motion to wave generation and propagation, this study developed an underwater soil-movement physical model test system. The system integrates controllable landslide initiation, real-time monitoring of landslide motion, wave height measurements, and full-field image acquisition, enabling synchronous observation of landslide movement and water body response. By controlling the main variables influencing submarine landslide dynamics, a series of physical model experiments were conducted to investigate water surface waves generated under different test conditions. The study examines the complete process from the initial water disturbance caused by submerged landslide motion to tsunami generation and propagation. The effects of landslide volume, particle size, initial submergence depth, and slope angle on tsunami parameters, including wave height, wave velocity, and wave period, were evaluated. Using 21 experimental datasets for each landslide type, namely, cohesionless sandy slides and muddy debris flows, empirical formulas for maximum surge height were established through dimensional analysis, SPSS (v25)-based multiple nonlinear regression, and validation against experimental results. The validation results show strong agreement between the empirical predictions and the physical model test data. Full article

Background/Objectives: Ulcerative colitis (UC) is a chronic inflammatory bowel disease in which alterations in the gut microbiota and dietary lipid composition play a central role; this study aimed to evaluate the effects of synbiotics, omega-3 polyunsaturated fatty acids, and their combination on clinical, macroscopic, microbiological, and histopathological outcomes in dextran sodium sulfate (DSS)-induced colitis in Wistar rats. Methods: Seventy-two male Wistar rats were randomly allocated to four groups (n = 18/group) and received 5% DSS in drinking water for eight days to induce colitis. Following DSS withdrawal and histological confirmation of colitis in sentinel animals, groups were treated for 8 days as follows: DSS (control), DSS-S (synbiotics, Ecologic^® 825), DSS-Ω3 (omega-3 fatty acid-enriched diet, ProSure^®), or DSS-S&Ω3 (combined therapy). Eight rats per group were sacrificed on days 4 and 8 post-DSS. Body weight, Disease Activity Index (DAI), distal colon length, hematologic parameters, bacterial translocation to the liver and mesenteric lymph nodes, histological colitis score, and myeloperoxidase (MPO)-positive cell counts were assessed. Results: DSS induced severe colitis characterized by diarrhea, rectal bleeding, and extensive mucosal erosions. After 8 days of treatment, the DSS-S&Ω3 group showed the greatest body-weight recovery (206.1→222.9 g, p < 0.05 vs. other groups), significantly preserved distal colon length, and the largest reduction in DAI (p < 0.05). Both the DSS-S and DSS-S&Ω3 groups demonstrated reduced bacterial translocation compared with DSS. The DSS-Ω3 group demonstrated persistent MPO-positive neutrophil infiltration compared with the DSS-S and DSS-S&Ω3 groups, whereas combined therapy was associated with lower MPO-positive cell counts. Histological colitis scores were significantly improved only in the DSS-S&Ω3 group (p < 0.05). Conclusions: In this DSS colitis model, the DSS-S&Ω3 group demonstrated superior clinical and histological outcomes compared with DSS-S or DSS-Ω3 alone, supporting further evaluation of combined synbiotic and omega-3 therapy as an adjunctive approach in ulcerative colitis. Full article

Background/Objectives: The purpose of this proof-of-concept study was to examine the effects oligosaccharide 6′-sialyllactose (6′-SL) supplementation (900 mg/d) during training on exercise performance and training adaptations in recreationally active males. Methods: In a randomized, double-blind design, 19 healthy males (24.4 ± 6.0 yrs, 174.9 ± 5.9 cm, 82.0 ± 15.2 kg, 27.1 ± 4.7 kg/m², 26.4 ± 6.9% body fat) ingested 3 × 300 mg/d of a placebo or 6′-SL for 12 weeks while partaking in a supervised resistance-training program while following their normal diet. Body composition (DXA), body water, submaximal lactate and substrate oxidation, 5RM dynamic muscular strength, ventilatory anaerobic threshold (VANT), peak aerobic capacity (VO₂), blood lactate, cycling anaerobic sprint capacity, and fasting blood samples were obtained at week 0, 6, and 12 of training and supplementation. Data were analyzed using multivariate and univariate general linear models (GLM) with repeated measures, along with assessments of mean changes from baseline and corresponding 95% confidence intervals. Results: Both groups observed positive training adaptations with no significant differences observed between groups in body composition, 5RM dynamic strength, or anaerobic sprint capacity. Significant interaction effects were observed VANT (p = 0.032), VO₂ at VANT (p = 0.028), and submaximal glucose and fat oxidation (p = 0.034) while time to reach peak VO₂ (p = 0.083), absolute (p = 0.075) and relative (p = 0.057) peak VO₂ approached significance. At Week 6, changes in time to peak effort (196 s [−16, 409], p = 0.068), absolute (0.76 L/min [−0.005, 1.53], p = 0.051) and relative (10.9 mL/kg/min [0.52, 21.5], p = 0.045), and fat oxidation (20.5% [3.1, 37.9], p = 0.023) were significantly greater in the 6′-SL group while VANT (−9.2% [−18.3, −0.04], p = 0.049), VO₂ at VANT (−4.8% [−9.8, 0.2], p = 0.06) and submaximal glucose oxidation values (−20.5% [−37.9, −3.1], p = 0.024) were lower with 6′-SL. After 12 weeks of training, VANT (−9.7% [−17.7, −1.5], p = 0.023) and VO₂ at VANT (−6.4% [−11.8, −1.0], p = 0.024) values were significantly lower in the 6′-SL group. No significant differences were observed in resting, submaximal, or maximal exercise blood lactate while the ratios of LDL to HDL (−0.27 [−0.53, −0.01], p = 0.042) and total cholesterol to HDL (−0.32 [−0.60, −0.04], p = 0.028) decreased significantly from baseline after 6 weeks of training with 6′-SL. Conclusions: 6′-SL supplementation did not promote greater gains dynamic strength, fat free mass or changes in body composition. However, while there was some evidence that 6′-SL supplementation influenced training-induced changes in aerobic capacity during the first six weeks, fewer effects were observed after 12 weeks. Moreover, several differences only approached significance in this small proof-of-concept study, so results should be viewed as exploratory and hypothesis generating for additional research. Full article

Multisource remote sensing data is utilised for the purpose of monitoring annual and interannual changes associated with climate change in the water bodies of the Chotts of Merouane and Melrhir, which are located in the Zone of Chotts in North Africa. These endorheic depressions are distinguished by recurrent flooding events of varying magnitude and frequency, which are contingent on fluctuations in climate parameters. It has been determined that certain cities located within the surrounding watersheds, such as Biskra, are subject to the intermittent threat of severe flooding. This has been shown to result in land degradation and soil salinisation during the drying-up process. A detailed examination of chronological data from the 1960s onwards reveals a decline in the frequency of flooding in Chott Melrhir in recent years. It is noteworthy that the region has not experienced any substantial flooding since 2020. This phenomenon is concomitant with the marked decline in precipitation levels observed in the region. Since 1980, there have been at least ten significant floods, resulting in varying degrees of damage and disruption. In contrast, Chott Merouane exhibits a more consistent hydrological pattern, with water flowing almost year-round due to wastewater and the drainage of the palm groves by the Oued Righ. Until the 1970s, the occurrence of flooding in the region was exclusively attributable to the direct overflow of the Biskra River and its tributaries. However, from the 1980s onwards, a new type of flooding emerged, linked to insufficient infiltration and drainage capacity in the soil and sewage systems during rainfall that was sometimes considered normal. The hydrological regime in the area has severe ramifications for the water supply and the state of the oases, which are vulnerable to salinisation. Full article

Precision microclimate management is critical for stabilizing oyster mushroom (P. ostreatus) production under variable farm conditions. This study evaluates Mushroom Kothi, an IoT-enabled cultivation system integrating low-cost sensors, automated control strategies, and cloud-based monitoring, across multiple agro-climatic zones and seasons in India (Bharat). Comparative trials with conventional farmer-managed systems show that Mushroom Kothi significantly reduces microclimate variability, improves yield consistency, enhances fruit body uniformity, and increases water-use efficiency without relying on energy-intensive cooling. The results demonstrate that biologically informed, automated environmental stabilization—rather than growth forcing—can support sustainable, smallholder-oriented precision mushroom cultivation. Full article

This study develops a GIS-based multicriteria decision analysis framework to assess the territorial suitability of onshore wind energy in Colombia. The proposed approach combines technical and socio-environmental suitability modelling with territorial interpretation based on conflict and governance, moving beyond conventional siting models focused mainly on wind resource availability and infrastructure proximity. The technical assessment included wind speed, wind power density, terrain slope, land cover, land use, and proximity to electrical grids, main roads, settlements, and water bodies. In addition, a National Conflict Index and a National Governance Index were constructed to represent broader territorial conditions that may affect project implementation. Quantitative variables, including wind speed, wind power density, terrain slope, and distance-based criteria, were transformed onto a common suitability scale using linear fuzzy membership functions, whereas qualitative variables, including land cover and land use, were incorporated through categorical reclassification. The National Conflict Index and National Governance Index were first constructed using CRITIC to obtain objective weights for their internal variables. Subsequently, the final onshore wind suitability criteria were weighted through the linear Best–Worst Method based on expert judgment. The standardized suitability layers and corresponding BWM-derived weights were integrated through weighted spatial overlay to generate a national suitability map, while the conflict and governance indices were used to interpret the territorial conditions associated with the resulting suitable areas. The results show a highly selective territorial pattern, with the most favorable areas concentrated mainly in La Guajira (1286.09 km²) and Cesar (574.45 km²), and more fragmented secondary opportunities in Nariño, Boyacá, Norte de Santander, Cundinamarca, Atlántico, and Magdalena. Three territorial intervention scenarios were identified: priority intervention, complementary or selective development, and low relative priority. The main contribution of the study is the articulation of a BWM-weighted technical and socio-environmental suitability model with CRITIC-based conflict and governance indices, offering a replicable framework to support strategic planning and public policy decisions for onshore wind deployment in Colombia. Full article

Basalt has been studied in recent years as a novel geological medium for CO₂ sequestration, thus enriching and expanding the carbon sink potential of geological bodies. In this study, four sets of CO₂–water–basalt interaction experiments were carried out for different ranges of temperature, pressure, particle size, and basalt type, while taking account of actual in situ temperature and pressure conditions in basalt strata. The aim was to provide a theoretical basis for the construction of future basalt CO₂ mineralization and storage projects. It was demonstrated that basalt begins to form calcitic minerals above 36 °C, with the highest carbonate mineral formation occurring at 66 °C accompanied by minor hydration of magnesium carbonate minerals. Below 26 °C, the dissolution of calcitic minerals showed a gradually increasing trend at higher pressures, with small amounts of hydrated magnesium carbonate minerals and calcite being formed at 9 MPa and 13 MPa. Maximum mineral dissolution occurred at 30–35 mesh particle size. The dissolution reaction in porous basalt was more intense for different basalt types (e.g., porous basalt and massive olivine basalt). Full article

Built-up area extraction is important for monitoring urban development and land-use change. Index-based methods are widely used for extracting built-up areas from Landsat imagery because of their simplicity and efficiency. However, conventional built-up indices often enhance bare land together with built-up areas due to their similar spectral characteristics, which reduces extraction accuracy and limits automatic threshold selection. To address this problem, this study proposes a built-up area extraction method based on multi-index synthesis and principal component analysis (PCA). First, NDBI (Normalization Differential Building Index), SAVI (Soil-Adjusted Vegetation Index), MNDWI (Modified Normalized Difference Water Index), and the brightness, greenness, and wetness components of the Tasseled Cap transformation were stacked to construct a six-band synthetic index image, enhancing the contrast among built-up areas, bare land, vegetation, and water bodies. PCA was then applied to the synthetic image using both correlation and covariance matrices, and the second principal component was used to enhance built-up area information. The resulting CorPC2 and CovPC2 methods were evaluated and compared with conventional built-up indices. The results showed that both PC2-based methods improved the separability between built-up areas and background features, while CovPC2 achieved the best performance by more effectively suppressing bare-land interference without requiring an additional bare-land mask. In the main experimental area, CovPC2 achieved higher accuracy than the comparison methods, and its Otsu-based result remained close to the optimal-threshold result. Validation in three typical cities further demonstrated the applicability of the proposed method across different Landsat sensors and urban environments. The proposed PC2-based method, particularly CovPC2, provides an effective and more automated approach for Landsat-based built-up area extraction under bare-land interference. Additionally, by using a threshold optimizing algorithm, built-up areas can be automatically extracted with high accuracy. Full article

Due to multiple anthropogenic drivers, coastal wetlands have lost roughly 50% of their historical coverage, and deterioration is accelerating with rising sea levels. Thin-layer placement (TLP), the spreading of sediment dredged from nearby water bodies across existing wetlands or shallow mudflats to raise surface elevation, has emerged as a viable approach to sustain and restore these habitats. Strategies for the prioritization of site selection and design elements for TLP interventions remain unclear; a gap that must be closed to coordinate dredging with wetland restoration efficiently, given time, financial, and sediment constraints. Here, we present a transferable workflow to plan TLP projects, including systematic assessment of restoration needs, development of sediment application options, and prioritization of project sites that leverage publicly available remote-sensing data products and stakeholder input. We demonstrate its applicability in a rapidly deteriorating salt marsh–mangrove co-dominated system on the Atlantic coast of Florida. Guided by stakeholder priorities for storm-surge mitigation and habitat improvement, we tracked long-term (1952–2023) changes in vegetated wetland coverage to quantify loss trends and establish historic habitat borders as restoration targets. We then summarized short-term (2010–2023) habitat-mosaic shifts to resolve plant-species composition changes. In our focal system, long-term analyses revealed hotspots (zones 1 and 7) of >35% vegetation loss, while short-term analyses showed a 180% mangrove expansion and cordgrass degradation across all zones, suggesting a nuanced, tailored approach to sediment application. Taken together, this workflow provides a data-driven, stakeholder-informed process for TLP site prioritization to restore threatened wetlands, bolster coastal resilience, and maximize stakeholder benefits in our demonstration system in northeast Florida and, more broadly, to other dynamic coastlines. Full article

Although Tenebrio molitor is increasingly explored as a feed ingredient, uncertainty remains regarding the effects of high dietary inclusion levels in model animals. Therefore, this study evaluated physiological and behavioral responses to graded inclusion levels of T. molitor meal (25–45%) in the diets of adult female Wistar rats. The assessed outcomes comprised (i) clinical parameters, (ii) food and water intake, (iii) hematology and serum biochemistry, and (iv) behavior. Across the insect-fed groups, no unintended weight loss or excessive gain was observed; instead, animals modulated their feed intake in a manner consistent with maintaining a relatively stable growth trajectory, without signs of hyperphagia suggestive of nutrient deficiency or anorexia indicative of adverse effects under study conditions. Diet acceptance remained high throughout the experiment, with no feed refusal or behavioral evidence of aversion that might be expected in response to poor palatability or discomfort. Heart rates and respiratory rates remained age-appropriate, showing typical temporal variability. Significant between-group differences were observed in RDW, Retic (%), NEU (%, K/μL), EOS, PLT, and PCT (p < 0.05), while serum analyses revealed significant differences in AST, total bilirubin, creatinine, phosphorus, and BUN (p < 0.05). A moderate negative correlation between individual feed intake and body weight was additionally identified in selected groups. The results of behavioral testing reveal that the dietary inclusion of T. molitor meal at levels up to 45% did not reduce spontaneous activity or elicit anxiety-like responses, and exploratory behavior appeared to be preserved. Overall, the present findings indicate that the gradual incorporation of T. molitor meal at high inclusion levels supported normal growth, physiological function, and behavior in rats under the conditions studied. Full article