Search Results (97,947)

Background/Objectives: 16S ribosomal RNA sequencing has, for several years, been the main means of identifying bacterial and archaeal species. Low-throughput Sanger sequencing is often used for the detection and identification of microbial species, but this technique has several limitations. The use of high-throughput sequencers may be a good alternative to improve patient identification, especially for polyclonal infections and management. Kraken 2 and KrakenUniq are free, high-throughput tools providing a very rapid and accurate classification for metagenomic analyses. However, Kraken 2 can present false-positive results relative to KrakenUniq, which can be limiting in hospital settings requiring high levels of accuracy. The aim of this study was to establish an alternative next-generation sequencing technique to replace Sanger sequencing and to confirm that KrakenUniq is an excellent analysis tool that does not present false results relative to Kraken 2. Methods: DNA was extracted from reference bacterial samples for Laboratory Quality Controls (QCMDs) and the V2-V3 and V3-V4 regions of the 16S ribosomal gene were amplified. Amplified products were sequenced with the Illumina 16S Metagenomic Sequencing protocol with minor modifications to adapt and sequence an Illumina 16S library with a small 500-cycle nano-flow cell. The raw files (Fastq) were analyzed on a commercial Smartgene platform for comparison with Kraken 2 and KrakenUniq results. KrakenUniq was used with a standard bacterial database and with the 16S-specific Silva138, RDP11.5, and Greengenes 13.5 databases. Results: Seven of the eight (87.5%) QCMDs were correctly sequenced and identified by Sanger sequencing. The remaining QCMD, QCMD6, could not be identified through Sanger sequencing. All QCMDs were correctly sequenced and identified by MiSeq with the commercial Smartgene analysis platform. QCMD6 contained two bacteria, Acinetobacter and Klebsiella. KrakenUniq identification results were identical to those of Smartgene, whereas Kraken 2 yielded 25% false-positive results. Conclusions: If Sanger identification fails, MiSeq with a small nano-flow cell is a very good alternative for the identification of bacterial species. KrakenUniq is a free, fast, and easy-to-use tool for identifying and classifying bacterial infections. Full article

Background and Objectives: Laryngeal cancer is a common head and neck tumor burden, with no significant improvements in long term patient survival. Despite the progress of molecular genetics and oncology strategies, there is still a lack of biomarker use in routine clinical practice for early laryngeal cancer screening or diagnosis. miRNAs are explored as promising molecules, that could serve as liquid biopsy. Our goal is to explore the screening potential of miR-31-3p and miR-196a-5p in early- and advanced-stage laryngeal HPV-negative plasma samples. Methods: In this study, 50 plasma samples obtained from early and advanced HPV-negative laryngeal cancer patients were included. The expression levels of mir-31-3p and miR-196a-5p were analyzed via TaqMan RT-qPCR. SPSS v27.0 was used for statistical analysis. Results: For the first time, miR-31-3p and miR-196a-5p were analyzed in plasma samples from early HPV-negative primary LSCC patients. Both circulating miRNAs showed significantly elevated expression levels in early and advanced laryngeal cancer samples. miR-31-3p was significantly associated with T stages (p < 0.001) and N stages (p = 0.009). The ROC analysis revealed that miR-31-3p could significantly discriminate early-stage from advanced-stage LSCC with an AUC of 0.850 (95% CI: 0.743–0.956, p < 0.001) at an RQ cutoff of 2.03, achieving a sensitivity of 95.5% and a specificity of 64%. Nevertheless, miR-196a-5p was found to be significantly overexpressed in early-stage LSCC, which could contribute to the development of its screening potential. For the first time, both miRNAs revealed a significant positive correlation, which indicates that miR-31-3p and miR-196a-5p could coregulate cancerogenesis. Conclusions: In conclusion, the data revealed that miR-31-3p has greater potential as an LSCC screening marker in comparison to miR-196a-5p. Still, miR-196a-5p also showed promising results in early-stage laryngeal cancer monitoring. The utilization of circulating miR-31-3p or miR-196a-5p analysis could enable liquid biopsy approaches, with results potentially informing treatment monitoring strategies, personalized oncological protocols, and early diagnosis. These advancements could ultimately benefit patient outcomes by improving laryngeal organ preservation and survival rates. Full article

Background/Objectives: The improvement of sleep quality is unquestionably a critical issue in public health. While numerous factors influence sleep quality, the relative importance of dietary habits remains insufficiently understood. The objective of this study is to evaluate the contribution of dietary habits by quantitatively comparing the effects of various determinants of sleep quality. Methods: Using sleep diary data from healthy males and females residing in a specific region of Japan, we estimated a dynamic multivariate panel model (DMPM) to obtain posterior predictive distributions on a scale that allows for comparisons across factor categories. Three outcome variables were adopted to measure sleep quality: presence or absence of daytime drowsiness, ease of falling asleep, and ease of waking up. The determinants of sleep quality examined in the analysis were categorized into six groups: stress factors, bedtime conditions, weather conditions, physical characteristics, exercise habits, and dietary habits. Results: The analysis revealed that although there were some seasonal and gender differences, dietary habits showed effect sizes that were no smaller than those of other determinants across all outcome variables. Conclusions: These results suggest that improving dietary habits, along with enhancing exercise habits and bedtime conditions, is a valid and equally important strategy for promoting better sleep. Full article

Marking techniques are employed to guarantee the identification and traceability of biomedical materials. This study investigated the impact of laser and mechanical marking processes on the tribological performance of ISO 5832-1 austenitic stainless steel (SS), specifically examining corrosion resistance, the coefficient of friction, and wear volume in ball-cratering wear tests. The laser marking was performed using a nanosecond Q-switched Nd:YAG laser. Cytotoxicity tests assessed the biocompatibility of the biomaterial. Non-marked surfaces were also evaluated for comparison. A phosphate-buffered saline solution (PBS) served as both the lubricant and corrosion medium. The surface finishing was analyzed using optical microscopy and scanning electron microscopy coupled with a field-emission gun (SEM-FEG), combined with an energy-dispersive X-ray spectrometer. The oxide film was examined through X-ray photoelectron spectroscopy (XPS). Wear tests lasted 10 min, with PBS drops applied every 10 s at 75 rpm; solid balls of AISI 316L stainless steel (SS) and polypropylene (PP), each 1 inch in diameter, were used as counter-bodies. Corrosion resistance was assessed using electrochemical methods. Results showed variations in roughness and microstructure due to laser marking. The tribological behaviour was influenced by the type of marking process, and the wear amount depended on the normal force and ball nature. None of the samples was considered cytotoxic, although laser-marked surfaces exhibited the lowest cellular viability among the tested surfaces and the lowest corrosion resistance. Full article

In 2021, the IPCC published new sea level projections. For the first time, the projections gave insight into expected relative sea level rise locally. A prudent designer of coastal infrastructure will want to know how the local projections compare to local observations. That comparison, to date, has not been made. We compared local projections and observations regarding the rate of rise in 2020. We used two datasets with local sea level information all over the globe. In both datasets, we found approximately 15% of the available sets suitable to establish the rate of rise in 2020. Geographic coverage of the suitable locations is poor, with the majority of suitable locations in the Northern Hemisphere. Latin America and Africa are severely under-represented. Statistical tests were run on all selected datasets, taking acceleration of sea level rise as a hypothesis. In both datasets, approximately 95% of the suitable locations show no statistically significant acceleration of the rate of sea level rise. The investigation suggests that local, non-climatic phenomena are a plausible cause of the accelerated sea level rise observed at the remaining 5% of the suitable locations. On average, the rate of rise projected by the IPCC is biased upward with approximately 2 mm per year in comparison with the observed rate. Full article

Although the dosimetric advantages of rectal spacers in prostate cancer radiotherapy have been demonstrated in selected clinical trials, real-world data from routine clinical practice remain limited—particularly within the Portuguese healthcare system. This study offers a detailed dosimetric comparison of Volumetric Modulated Arc Therapy (VMAT), with and without rectal spacer use, in a real-world patient cohort, aiming to assess the clinical relevance of spacer insertion under standard treatment protocols. A retrospective dosimetric evaluation was performed on 80 prostate cancer patients treated at a radiotherapy centre in southern Portugal. Patients were equally divided into two matched groups (n = 40): one receiving VMAT alone, the other receiving VMAT with hydrogel rectal spacer placement. Dose-volume histograms (DVHs) were analysed for the planning target volume (PTV) and key organs at risk (OARs). Standard dosimetric metrics, such as V50–V75 for the rectum and bladder, V50 for femoral heads, and mean dose for the penile bulb, were assessed. PTV coverage was evaluated using conformity and homogeneity indices. Spacer use significantly decreased rectal dose exposure across all evaluated parameters without compromising PTV coverage or increasing dose to other OARs. These findings support routine rectal spacer applications to enhance treatment safety and patient outcomes. Full article

(1) Background: Water, comprising about 70–80% of cellular mass, is the most abundant constituent of living cells. Upon exposure to ionizing radiation, water undergoes radiolysis, generating a variety of reactive species, including free radicals and molecular products. Among these, hydroxyl radicals (^•OH) are particularly damaging due to their very high reactivity and their capacity to induce oxidative injury to vital biomolecules such as DNA, membrane lipids, and proteins. From a radiation-chemical perspective, this study investigates the selective scavenging ability of molecular hydrogen (H₂) toward ^•OH radicals, with the aim of evaluating its potential as an antioxidant and radioprotective agent; (2) Methods: We employed our Monte Carlo track chemistry simulation code, IONLYS-IRT, to model the time-dependent yields of ROS in a neutral, aerated aqueous environment. The simulations included varying concentrations of dissolved H₂ and, for comparison, cystamine—a well-known sulfur-containing radioprotector and antioxidant. Irradiation was simulated using 300 MeV protons, chosen to mimic the radiolytic effects of low linear energy transfer (LET) radiation, such as that of ⁶⁰Co γ-rays or fast (>1 MeV) electrons; (3) Results: Our simulations quantitatively demonstrated that H₂ selectively scavenges ^•OH radicals. Nevertheless, its scavenging efficiency was consistently lower than that of cystamine, which produced a faster and more pronounced suppression of ^•OH due to its higher reactivity and superior radical-quenching capacity; (4) Conclusions: Molecular hydrogen offers several unique advantages, including low toxicity, high diffusivity, selective scavenging of ^•OH radicals, and well-documented anti-inflammatory effects. Although it is less potent than cystamine in terms of radical-scavenging efficiency, its excellent safety profile and biological compatibility position H₂ as a promising radioprotector and antioxidant for therapeutic applications targeting radiation-induced oxidative stress and inflammation. Full article

Background/Objectives: Cardiac amyloidosis (CA) and cardiac sarcoidosis (CS) are two distinct infiltrative cardiomyopathies that can present with overlapping clinical features, including heart failure and arrhythmias. However, they arise from fundamentally different pathophysiological mechanisms: amyloid protein deposition in CA versus granulomatous inflammation in CS. These differing pathophysiologies result in divergent imaging patterns, clinical trajectories, and treatment strategies. This study aims to compare the clinical presentations, imaging characteristics, and outcomes of patients with CA and CS to identify key differentiating factors that can improve diagnostic precision and guide therapy. Methods: This single-center, retrospective, cross-sectional study analyzed electronic medical records of patients diagnosed with CA (limited to transthyretin CA) or CS at Mount Sinai Morningside system from January 2017 until October 2023. Patients were identified using diagnostic codes and confirmed by histology or disease-specific imaging criteria. Clinical data, transthoracic echocardiography (TTE), cardiac magnetic resonance (CMR) imaging, pyrophosphate scintigraphy (PYP), and fluorodeoxyglucose positron emission tomography (FDG-PET) findings were collected. Statistical comparisons between groups were performed using chi-square tests and independent t-tests, with p < 0.05 considered statistically significant. Results: A total of 16,834 patients were screened and 216 patients were included in the analysis (125 CA, 92 CS). CA patients were older (78.2 vs. 62.0 years, p = 0.01), had greater interventricular septal thickness (1.57 vs. 1.10 cm, p = 0.01), and exhibited diffuse late gadolinium enhancement (LGE) and elevated extracellular volume (ECV) on CMR. CS patients had higher rates of ventricular tachycardia (53.3% vs. 10.7%, p = 0.01), increased myocardial fluorodeoxyglucose (FDG) uptake on positron emission tomography (PET) (90%), and more frequent implantable cardioverter-defibrillator (ICD) placement (66.3% vs. 13.0%, p = 0.01). Conclusions: CA and CS demonstrate distinct imaging profiles, arrhythmic risks, and treatment patterns. Early differentiation using advanced imaging is crucial for implementing disease-modifying therapies in CA and for immunosuppression and ICD implantation in CS, thereby improving patient outcomes. Full article

Given its considerable cultural, historical, and economic value, built heritage requires the application of modern techniques for effective documentation and conservation. While multiple sensors are available for 3D modeling, laser scanning remains the most commonly employed due to its efficiency, precision, and ability to comprehensively capture the building’s geometry, surface textures, and structural details. This results in highly detailed 3D representations that are very important for accurate documentation, analysis, and conservation planning. This study investigates the complementary potential of different 3D modeling approaches for the digital representation of the Dosoftei House in Iasi, a monument of historical significance. For this purpose, an integrated point cloud was created based on a mobile hand-held laser scanner (HMLS), i.e., the FJD Trion P1 and a terrestrial laser scanner (TLS), i.e., the Maptek I-Site 8820 long-range laser scanner, the latter specifically used to capture the roof structures. Based on this dataset, a parametric model was created in Revit, supported by panoramic images, allowing for a structured representation useful in technical documentation and heritage management. In parallel, a mesh model was generated in CloudCompare using Poisson surface reconstruction. The comparison of the two methods highlights the high geometric accuracy of the mesh model and the Building Information Modeling (BIM) model’s capability to efficiently manage information linked to architectural elements. While the mesh provides detailed geometry, the BIM model excels in information organization and supports informed decision-making in conservation efforts. This research proposes leveraging the advantages of both methods within an integrated workflow, applicable on a larger scale in architectural heritage conservation projects. Full article

Background: Colovesical fistulas are abnormal communications between the colon and urinary bladder, most commonly caused by diverticular disease. Although colovesical fistulas are rare, they should be suspected in patients presenting with recurrent urinary tract infections, pneumaturia, or fecaluria. We integrated two case reports with a retrospective national cohort analysis to assess the surgical treatment of colovesical fistulas. Methods: We report two cases of colovesical fistulas, both secondary to sigmoid diverticulitis, treated surgically via minimally invasive approaches. A retrospective analysis using the National Inpatient Sample database from 2016 to 2022 was conducted to compare outcomes of open surgery with those of minimally invasive surgery. Propensity score matching and multivariable regression analyses were used to evaluate clinical outcomes. Results: The first patient underwent hand-assisted laparoscopic sigmoidectomy with fistula takedown and has remained asymptomatic at 8 months, while the second patient underwent robotic-assisted sigmoidectomy with staged ileostomy reversal and has remained asymptomatic at 1 month. National data analysis showed no significant difference in mortality (<1% versus <1%, p = 0.931), wound complications (1.4% versus 1.0%; p = 0.554), or postoperative sepsis or shock (7.1% versus 5.6%; p = 0.114) between open and minimally invasive surgical approaches. However, the minimally invasive surgery group had significantly shorter length of stay than the open surgery group (6.9 versus 7.3 days, p < 0.001). Conclusions: Minimally invasive repair of colovesical fistulas was associated with shorter hospital stays than open surgery, with no significant differences in major complications. Early identification and timely surgical management are critical for achieving favorable outcomes. Full article

In the current context where China is continuously emphasizing the construction and supply of indemnificatory housing, and actively promoting the construction of “Better Housing” for such housing, the development experiences of Japan and South Korea in the field of public housing reveal that the construction and supply of public housing cannot be separated from the interaction and coordinated development of the policy system, spatial composition, and cultural factors. Based on this, this study takes the public rental housing in China, Japan, and South Korea as the research objects, through comparative analysis of their policy systems, cultural backgrounds, and spatial composition characteristics of unit plans, to explore the implications for the development of China’s indemnificatory housing, and provides theoretical basis and practical references for optimizing the supply system and space design of China’s indemnificatory housing. The study selects typical cases of public rental housing from the three countries, and conducts comparisons from dimensions such as unit plane shape, L.D.K. layout, bedroom configuration, transitional space, balcony design, and bathroom composition. Findings indicate that Japan’s UR rental housing focuses on refined and diversified design, South Korea’s public housing emphasizes spatial flexibility, while China’s indemnificatory housing, while pursuing standardized construction, faces challenges of area limitations and insufficient functional adaptability. Based on the experiences of the three countries, this study proposes a tripartite guidance suggestion of “Policy–Space–Culture” to advance the realization of “Better Housing” objectives and ensure that China’s indemnificatory housing meets both international advanced experience and local social and cultural specific needs: (1) policy systems—strengthening legalization and long-term sustainability in governance; (2) spatial composition—incorporating flexible layouts and human-centric detailing; (3) cultural adaptability—balancing traditional living habits with contemporary needs. Full article

Traffic violations, the primary cause of road accidents, threaten public safety by disrupting traffic flow and causing substantial casualties and economic losses. Accurate spatiotemporal prediction of violations offers critical insights for proactive traffic management. While Graph Attention Network (GAT) methods excel in spatiotemporal forecasting, their practical deployment is hindered by prohibitive computational costs when handling dynamic large-scale data. To address this issue, we propose a Lightweight Traffic Violation Prediction with Graph Attention Distillation (LTVPGA) model, transferring spatial topology comprehension from a complex GAT to an efficient multilayer perceptron (MLP) via knowledge distillation. Our core contribution lies in topology-invariant knowledge transfer, where spatial relation priors distilled from the teacher’s attention heads enable the MLP student to bypass explicit graph computation. This approach achieves significant efficiency gains for large-scale data—notably accelerated inference time and reduced memory overhead—while preserving modeling capability. We conducted a performance comparison between LTVPGA, Conv-LSTM, and GATR (teacher model). LTVPGA achieved revolutionary efficiency: consuming merely 15% memory and 0.6% training time of GATR while preserving nearly the same accuracy. This capacity enables practical deployment without sacrificing fidelity, providing a scalable solution for intelligent transportation governance. Full article

In this study, data from 17 studies reporting the presence of microplastics in milk and dairy products in the literature were examined with a product-based systematic approach. In addition, geographical comparisons were made between different countries. In milk and dairy products, the concentration of microplastics has been reported to exhibit a broad range, extending from non-detectable levels to as high as 10,040 MPs per kilogram, contingent upon the specific product types. Milk powder (especially baby milk powder) stands out as the riskiest product group in terms of microplastic content. Although the sizes and colors of the detected microplastics vary significantly, the fiber form is generally predominant. While polyethylene, polypropylene, polyamide and polyester are among the polymers frequently detected, high-temperature-resistant industrial polymers such as polytetrafluoroethylene, polysulfone, polyurethane were also encountered. In addition, the presence of some polymers (such polyvinyl chloride, polyurethane) that are toxicologically risky for human health was reported in the studies. In addition, the study evaluated the chemical, enzymatic and physical methods used for the separation and identification of MPs; the advantages and limitations of FT-IR, Raman and other analysis techniques were revealed. This study reveals that MP contamination in milk and dairy products is a multidimensional problem. The findings show that milk and dairy products are highly susceptible to plastic contamination at every stage of production. Full article

This study presents a detailed investigation of single-mode–multimode–single-mode (SMS) fiber sensors with varied cladding thicknesses of multimode fiber (MMF) (4, 3, 2, 1, and 0 μm). The refractive index (RI) sensing performance of these sensors was evaluated using sensitivity, limit of detection (LOD), full width at half maximum (FWHM), quality factor (Q-factor), figure of merit (FOM), and comprehensive evaluation index (CEI). The results demonstrate that the sensor with a multimode fiber (MMF) cladding thickness of 0 μm achieves the highest overall performance, characterized by a superior sensitivity of 106.00 nm/RIU and a maximum figure of merit (FOM) of 5.54 RIU⁻¹. In comparison, the sensor with a 2 μm MMF cladding thickness exhibits minimized energy loss, resulting in the highest quality factor of 169.02 and the narrowest full width at half maximum of 8.02 nm. Additionally, sensor stability is found to be independent of MMF cladding thickness. Full article

The objective of the present study was to compare the diagnostic performance of radiography to computed tomography (CT) for the diagnosis of distal tarsal osteoarthritis. All images were interpreted and scored by 2 evaluators until a consensus was reached; they were blinded to the history, final imaging diagnosis, and results of the corresponding radiographic or CT study. On radiographs and CT images of 54 tarsi, 6 criteria (osteophyte/enthesophyte height, small/large subchondral bone radiolucencies, subchondral bone thickness, joint space narrowing) were scored in the proximal intertarsal (PIJ), distal intertarsal (DIJ), and tarsometatarsal joint (TMTJ). Compared to CT, mean radiographic scores were significantly lower for 1. small and large subchondral bone radiolucencies in all joints (p < 0.001–0.03); 2. subchondral bone thickness in the PIJ and DIJ (p = 0.03 and 0.005); and 3. enthesophyte and joint space narrowing score in the DIJ (p = 0.04 and 0.002). Low-to-high positive predictive values (PPV) were calculated for the individual criteria in all joints (0–100%). For the sum of scores of all criteria in the PIJ, DIJ, or TMTJ, respectively, PPVs were high (94–98%). In conclusion, radiography is a useful screening tool provided multiple criteria are assessed. However, CT offers significant advantages for the diagnosis of distal tarsal OA. Full article