Search Results (5,052)

Leptospirosis is a globally distributed zoonosis of major public health and veterinary relevance, caused by pathogenic species of the genus Leptospira. Brazil is a hotspot for transmission due to its ecological diversity and complex host–environment interfaces. This study explored the genetic diversity and structure of circulating pathogenic Leptospira spp. in Brazil through a single-locus sequence typing (SLST) analysis based on the secY gene. A total of 531 sequences were retrieved from GenBank and subjected to phylogenetic and haplotype diversity analyses. Maximum likelihood reconstruction revealed strongly supported clades for seven species, with L. interrogans being the most prevalent and broadly distributed across hosts and regions. This species showed evidence of clonal expansion, with a dominant haplotype (n = 242) shared by humans, domestic animals, and wildlife. In contrast, L. santarosai and L. noguchii exhibited high haplotypic diversity and reticulated network structures, reflecting greater evolutionary variability. The species L. kirschneri and L. borgpetersenii displayed reduced haplotypic variation, the latter mainly associated with cattle, consistent with its host-adapted profile. Host- and biome-based haplotype networks revealed both the broad ecological adaptability of certain lineages and the exclusive presence of haplotypes restricted to specific environments, such as those found in marine mammals from the Atlantic Ocean. Genetic distance analyses confirmed the strong taxonomic resolution of the gene secY, which effectively distinguished closely related species while capturing intraspecific diversity. These findings provide a comprehensive molecular overview of pathogenic Leptospira in Brazil, highlighting ecological connectivity across hosts and biomes, as well as the contrasting evolutionary dynamics among species. Beyond describing genetic patterns, our analyses emphasize evolutionary processes, host–environment connectivity, and the implications for One Health. This integrative framework strengthens the basis for surveillance and control strategies in other endemic regions in the world. Full article

The application of Artificial Intelligence (AI) in river basin pollution control shows great potential to improve governance efficiency through real-time monitoring, pollution prediction, and intelligent decision-making. However, its rapid development also brings regulatory challenges, including data privacy, algorithmic bias, responsibility definition, and cross-regional coordination. Based on the SETO loop framework (Scoping, Existing Regulation Assessment, Tool Selection, and Organizational Design), this paper systematically analyzes the regulatory needs and pathways for AI in watershed water pollution control through typical case studies from countries such as China and the United States. The study first defines the regulatory scope, focusing on protecting the ecological environment, public health, and data security. It then assesses the shortcomings of existing environmental regulations in governing AI, such as their inability to adapt to dynamic pollution sources. Subsequently, it explores suitable regulatory tools, including information disclosure requirements, algorithmic transparency standards, and hybrid regulatory models. Finally, it proposes a multi-tiered organizational scheme that integrates international norms, national legislation, and local practices to achieve flexible and effective regulation. This study demonstrates that the SETO loop provides a viable framework for balancing technological innovation with risk prevention and control. It offers a scientific basis for policymakers and calls for establishing a dynamic, layered regulatory system to address the complex challenges of AI in environmental governance. Full article

This study investigates spatiotemporal b value variations and seismic interaction networks preceding the M_wg 7.1 Dingri earthquake that struck southern Tibet on 7 January 2025. Using relocated earthquake catalogs (2021–2025) and dual-method analysis combining b value mapping with Granger causality network modeling, we reveal systematic precursory patterns. Spatial analysis shows that the most significant b value reduction (Δb > 0.5) occurred north of the mainshock epicenter at seismogenic depths (5–15 km), closely aligning with subsequent aftershock concentration zones. Granger causality analysis reveals a progressive network simplification: from 73 causal links among 28 nodes during the background period (2021–2023) to 49 links among 34 nodes pre-mainshock (2023–2025) and finally to 6 localized links post-rupture. This transition from distributed system-wide interactions to localized “locked-in” dynamics reflects the stress concentration onto the primary asperity approaching critical failure. The convergence of b value anomalies and network evolution provides a comprehensive framework linking quasi-static stress states with dynamic system behavior. These findings offer valuable insights for understanding earthquake nucleation processes and improving seismic hazard assessment in the Tibetan Plateau and similar complex tectonic environments. Full article

Continual learning (CL) is a key technology for enabling data-driven autonomous guidance systems to operate stably and persistently in complex and dynamic environments. Its core goal is to enable the model to continuously learn new scenarios and tasks after deployment, without forgetting existing knowledge, and finally achieving stable decision-making in the different scenarios over a long period. This paper proposes a continual learning method that combines feature-generation-replay with Mixture-of-Experts and Low-Rank Adaptation (MoE-LoRA). This method retains the key features of historical tasks by feature repla and realizes the adaptive selection of old and new knowledge by the Mixture-of-Experts (MoE), which alleviates the conflict between knowledge while ensuring learning efficiency. In the comparison experiments, we compared the proposed method with the representative continual learning methods, and the experimental results show that our method outperforms the representative continual learning methods, and the ablation experiments further demonstrate the role of each component. This work provides technical support for the long-term maintenance and new task expansion of data-driven autonomous guidance systems, laying a foundation for their stable operation in complex, variable real-world scenarios. Full article

Over the past decade, the rapid development of geospatial tools has significantly expanded the scope of archaeobotanical research, enabling unprecedented insights into ancient plant domestication, agricultural practices, and human-environment interactions. Within the Chinese context, where rich archaeobotanical records intersect with complex socio-ecological histories, GIS-driven approaches have revealed nuanced patterns of crop dispersal, settlement dynamics, and landscape modification. However, despite these advances, current applications remain largely exploratory, constrained by fragmented datasets and underutilized spatial-statistical methods. This paper argues that a more robust integration of large-scale archaeobotanical datasets with advanced ArcGIS functionalities—such as kernel density estimation, least-cost path analysis, and predictive modelling—is essential to address persistent gaps in the field. By synthesizing case studies from key Chinese Neolithic and Bronze Age sites, we demonstrate how spatial analytics can elucidate (1) spatiotemporal trends in plant use, (2) anthropogenic impacts on vegetation, and (3) the feedback loops between subsistence strategies and landscape evolution. Furthermore, we highlight the challenges of data standardization, scale dependency, and interdisciplinary collaboration in archaeobotanical ArcGIS. Ultimately, this study underscores the imperative for methodological harmonization and computational innovation to unravel the intricate relationships between ancient societies, agroecological systems, and long-term environmental change. Full article

Monitoring electrocardiogram (ECG) and respiration continuously and non-invasively is essential for managing cardiopulmonary health. An effective wearable device can be used to regularly monitor key vitals, reducing the need for clinical visits. In this work, we propose a custom device for real-time continuous ECG by inkjet printed (IJP) dry electrodes and respiration monitoring by using a novel single 6-axis inertial measurement unit (IMU). The proposed system can extract the heart rate (HR) and respiration rate (RR) during static and dynamic postures. The respiration process implements a quaternion-based update and multiple filtering stages to estimate the signal. The custom device uses Bluetooth protocol to send the raw and processed data to a mobile application. The RR is investigated in stationary, i.e., sitting and standing, and dynamic, i.e., walking, running, and cycling, postures. The proposed device is evaluated with commercial Go Direct^® respiration belt from Vernier^® for RR and offers an overall accuracy of 99.3% and 98.6% for static and dynamic conditions, respectively. The wearable also offers 98.9% and 97.9% accuracy for HR measurements, respectively, in static and active postures when compared with the Kardia^® device. Furthermore, the device is assessed in an ambulatory monitoring setup in both indoor and outdoor environments. The low-power wearable consumes an average of only 7.4 mA of current during data processing. The device performs effectively and efficiently in both stationary and active states, offering a low complexity, portable solution for real-time monitoring. The proposed system can benefit from the continuous monitoring and early detection of pulmonary and cardio-respiratory health issues. Full article

Besides genomic and proteomic analyses of bulk and individual cancer cells, cancer research focuses on the mechanical analysis of cancers, such as cancer cells. Throughout the oncogenic evolution of cancer, mechanical inputs are stored as epigenetic memory, which ensures versatile coding of malignant characteristics and a quicker response to external environmental influences in comparison to solely mutation-based clonal evolutionary mechanisms. Cancer’s mechanical memory is a proposed mechanism for how complex details such as metastatic phenotypes, treatment resistance, and the interaction of cancers with their environment could be stored at multiple levels. The mechanism appears to be similar to the formation of memories in the brain and immune system like epigenetic alterations in individual cells and scattered state changes in groups of cells. Carcinogenesis could therefore be the outcome of physiological multistage feedback mechanisms triggered by specific heritable oncogenic alterations, resulting in a tumor-specific disruption of the integration of the target site/tissue into the overall organism. This review highlights and discusses the impact of the ECM on cancer cells’ mechanical memory during their metastatic spread. Additionally, it demonstrates how the emergence of a mechanical memory of cancer can give rise to new degrees of individuality within the host organism, and a connection to the cancer entity is established by discussing a connection to the metastasis cascade. The aim is to identify common mechanical memory mechanisms of different types of cancer. Finally, it is emphasized that efforts to identify the malignant potency of tumors should go way beyond sequencing approaches and include a functional diagnosis of cancer physiology and a dynamic mechanical assessment of cancer cells. Full article

Background/Objectives: Lipid nanoparticles (LNPs) have demonstrated notable clinical success as advanced drug delivery systems. However, the development of novel covalently bonded ionizable lipids faces substantial technical challenges, as their modification is difficult and they have a high molecular weight. To address this issue, we report the use of host–guest complexes in supramolecular chemistry as functional lipid motifs for constructing LNPs. Methods: Ionizable amine β-cyclodextrin (amine β-CD)-derived host–guest amphiphilic lipid molecules (HGLs) were designed for the construction of multi-stage assembly supramolecular LNPs (MSLNPs). The structure–function relationships and stability of MSLNPs were explored by screening eight types of amine β-CDs and varying the ratio of HGL to yolk phosphatidylcholine. Stability screening and molecular dynamics simulations were performed to clarify the self-assembly mechanisms and optimal formulations, followed by a systematic evaluation of delivery performance. Results: MSLNPs showed a high drug-loading efficiency (> 30%), a rapid-response release in acidic environments, and multi-pathway cellular uptake. In vivo delivery experiments using ethylenediamine β-CD-based MSLNPs in mice revealed no significant immunogenicity, no significant abnormalities in organs/tissues or their functions, a unique biodistribution pattern, and pronounced renal targeting. The successful development of MSLNPs with acidic pH-responsive control, a high delivery efficiency, and renal-targeting properties simplifies LNP preparation. Conclusions: This study offers novel insights into the design of simplified LNPs and the optimization of targeted delivery, with potential applications in renal disease therapy. Full article

The human skin microbiome plays a crucial role in maintaining skin health by acting as a barrier against pathogens and modulating immune regulation. This case study investigates the skin microbiome of two healthy Korean male individuals in their 20s during Antarctic expeditions, focusing on microbial changes, reversion to pre-expedition states, and the influence of environmental and lifestyle factors. Notable microbial alterations were observed, including increases in Pseudomonadota and decreases in Actinomycetota, indicating pronounced microbial shifts in response to harsh environmental factors such as low temperature and humidity. Post-expedition revealed incomplete recovery to pre-expedition states, with Host A showing a higher resilience index, suggesting faster microbial recovery. Correlation analyses revealed associations between microbial changes and environmental factors (e.g., temperature, humidity, atmospheric pressure) as well as lifestyle factors (e.g., sunblock usage, outdoor activities), highlighting complex interactions between host behaviors and microbiome dynamics. Despite the study’s limited sample size, these findings offer insights into the adaptability and resilience of the skin microbiome under extreme environments, with potential implications for health management and skincare strategies during isolated and prolonged expeditions. Full article

This study proposes a discrete multi-agent Q-learning framework for the online tuning of PID controllers in continuous dynamic systems with limited observability. The approach treats the adjustment of each PID gain ($k_p$, $k_i$, $k_d$) as an independent learning process, in which each agent operates within a discrete state space corresponding to its own gain and selects actions from a tripartite space (decrease, maintain, or increase its gain). The agents act simultaneously under fixed decision intervals, favoring their convergence by preserving quasi-stationary conditions of the perceived environment, while a shared cumulative global reward, composed of system parameters, time and control action penalties, and stability incentives, guides coordinated exploration toward control objectives. Implemented in Python, the framework was validated in two nonlinear control problems: a water-tank and inverted pendulum (cart-pole) systems. The agents achieved their initial convergence after approximately 300 and 500 episodes, respectively, with overall success rates of $49.6\%$ and $46.2\%$ in 5000 training episodes. The learning process exhibited sustained convergence toward effective PID configurations capable of stabilizing both systems without explicit dynamic models. These findings confirm the feasibility of the proposed low-complexity discrete reinforcement learning approach for online adaptive PID tuning, achieving interpretable and reproducible control policies and providing a new basis for future hybrid schemes that unite classical control theory and reinforcement learning agents. Full article

In the context of cybersecurity digital twin environments, the ability to simulate realistic network traffic is critical for validating and training intrusion detection systems. However, generating synthetic data that accurately reflects the complex, time-dependent nature of network flows remains a significant challenge. This paper presents an AI-based data generation approach designed to generate multivariate temporal network flow data that accurately reflects adversarial scenarios. The proposed method integrates a Long Short-Term Memory (LSTM) architecture trained to capture the temporal dynamics of both normal and attack traffic, ensuring the synthetic data preserves realistic, sequence-aware behavioral patterns. To further enhance data fidelity, a combination of deep learning-based generative models and statistical techniques is employed to synthesize both numerical and categorical features while maintaining the correct proportions and temporal relationships between attack and normal traffic. A key contribution of the framework is its ability to generate high-fidelity synthetic data that supports the simulation of realistic, production-like cybersecurity scenarios. Experimental results demonstrate the effectiveness of the approach in generating data that supports robust machine learning-based detection systems, making it a valuable tool for cybersecurity validation and training in digital twin environments. Full article

Conventional power load forecasting frameworks face limitations in dynamic spatial topology capture and long-term dependency modeling. To address these issues, this study proposes a hybrid GAT-CNN-LSTM architecture for enhanced short-term power load forecasting. The model integrates three core components synergistically: Graph Attention Network (GAT) dynamically captures spatial correlations via adaptive node weighting, resolving static topology constraints; a CNN-LSTM module extracts multi-scale temporal features—convolutional kernels decompose load fluctuations, while bidirectional LSTM layers model long-term trends; and a gated fusion mechanism adaptively weights and fuses spatio-temporal features, suppressing noise and enhancing sensitivity to critical load periods. Experimental validations on multi-city datasets show significant improvements: the model outperforms baseline models by a notable margin in error reduction, exhibits stronger robustness under extreme weather, and maintains superior stability in multi-step forecasting. This study concludes that the hybrid model balances spatial topological analysis and temporal trend modeling, providing higher accuracy and adaptability for STLF in complex power grid environments. Full article

This research addresses the complex challenge of integrating modern public transport into historic medieval city centers. These unique urban environments are characterized by narrow streets, protected heritage status, and topographical constraints, which are incompatible with conventional transit vehicles. The introduction of standard bus routes often aggravates traffic congestion and fails to meet the specific mobility needs of residents and visitors. This paper suggests that autonomous electric buses represent a viable and sustainable solution, capable of navigating these constrained environments while aligning with modern energy efficiency goals. The central challenge lies in the optimal selection of an autonomous electric bus that can operate safely and efficiently within the tight streets of historic city centers while satisfying the travel demands of passengers. To address this, a comprehensive study was conducted, analyzing resident mobility patterns—including key routes and hourly passenger loads—and the specific geometric constraints of the road network. Based on this empirical data, a vehicle dynamics model was developed in Matlab^®. This model simulates various operational scenarios by calculating the instantaneous forces (rolling resistance, aerodynamic drag, inertial forces) and the corresponding power required for different electric bus configurations to follow pre-established speed profiles. The core of this research is an optimization analysis, designed to identify the balance between minimizing total energy consumption and maximizing the quality of passenger service. The findings provide a quantitative framework and clear procedures for urban planners to select the most suitable autonomous transit system, ensuring that the chosen solution enhances mobility and accessibility without compromising the unique character of historic cities. Full article

To address the serious ecological problems caused by long-term mining in the Muli Coalfield, a three-year ecological restoration project was initiated in 2020. The Jvhugeng mining area was the largest and most ecologically damaged area in the Muli Coalfield. Vegetation restoration is the core of mine ecological restoration. Scientific evaluation of the vegetation restoration status in the Jvhugeng mining area is significant for comprehensively revealing ecological restoration effectiveness in the Muli Coalfield. Based on Sentinel-2’s spectral and temporal advantages and GF-1/GF-6’s high spatial resolution in detailed portrayal, fractional vegetation cover (FVC) and landscape pattern index were determined separately. Thus, the vegetation restoration effectiveness and spatiotemporal dynamics of the Jvhugeng mining area from 2020 to 2023 were evaluated in terms of structural and functional dimensions. The results show that, from 2020 to 2023, vegetation cover extent (varying from 8.77 km² in 2020 to a peak of 17.93 km² in 2022 and then decreasing to 13.48 km² in 2023) and FVC (from 0.33 in 2020 to about 0.50 during 2021–2023) first increased sharply and then fluctuated. Vegetation regions with both high FVC and dominant landscape features also presented the characteristics of rapid expansion and then fluctuation. Vegetation restoration demonstrated significant effectiveness, with the natural ecological environment restored to some extent and remaining stable. Newly vegetated regions had high FVC and significant landscape pattern characteristics. However, vegetation cover expansion also led to further fragmentation and morphological complexity of vegetation landscape patterns in the study area. The results can provide a basis for quantitatively assessing ecological restoration effectiveness in the Jvhugeng mining area and even the Muli Coalfield. This can also provide a dual-source data synergy technical reference for dynamic monitoring and effective evaluation of vegetation restoration in other mining areas. Full article

Three-dimensional path planning in emergency logistics is a complex optimization problem, particularly challenging because it requires considering conflicting objectives such as flight time, energy consumption, and obstacle avoidance. Unlike most urban logistics research, this study examines emergency delivery path planning in mountainous environments during natural disasters. One of the most effective approaches to this problem is to employ multi-objective evolutionary algorithms. However, while multi-objective genetic algorithms can handle multiple conflicting objectives, they struggle when dealing with complex constraints. This paper proposes a multi-objective genetic optimization method, Adaptive Crossover-Mutation Multi-Objective Genetic Optimization (ACM-NSGA-II), based on the classic NSGA-II framework. Inspired by the principle of symmetry, this method dynamically adjusts the mutation and crossover rates based on population diversity to maintain a balanced exploration–exploitation trade-off. When population diversity is low, the mutation rate is increased to promote exploration of the solution space; when population diversity is high, the crossover rate is increased to promote better information exchange. The algorithm maintains symmetry by gradually adjusting the step size, balancing adaptability and stability. To address the obstacle avoidance problem, we introduced a dynamic path repair strategy that respects the symmetry of no-fly zone boundaries and terrain features, ensuring the safety and efficiency of Unmanned Aerial Vehicles. This algorithm jointly optimizes three objectives: safety cost, flight time, and energy consumption. The algorithm was tested in a mountainous environment model simulating a remote area. In experiments, ACM-NSGA-II was compared with several mainstream evolutionary algorithms. The Pareto set and hypervolume metrics of each method were recorded and statistically analyzed at a 5% significance level. The results show that ACM-NSGA-II outperforms the baseline algorithms in terms of diversity, convergence, and feasibility. Specifically, compared with the traditional NSGA-II, ACM-NSGA-II improved the average hypervolume metric by 53.39% and reduced the average flight time by 24.26%. ACM-NSGA-II also demonstrated significant advantages over other popular standard algorithms. Experimental results show that it can effectively solve the path planning challenge of emergency logistics Unmanned Aerial Vehicles in mountainous environments. Full article