Search Results (351)

Reproductive performance is a key determinant of overall livestock productivity. In both beef and dairy systems, reproductive failure represents a leading cause of cow culling. Reproductive traits are complex in nature and present a low heritability in general. Additionally, the collection of such phenotypes usually relies on indirect measures of fertility, such as conception success. Therefore, further investigation into genetic and non-genetic factors of reproductive traits in cattle is necessary. The hosts’ microbiome plays a crucial role in vertebrate biology, including reproduction. We, therefore, hypothesize that microbiome indicators may serve as a biomarker of fertility. This study explored the relationship between vaginal microbiome profiles and pregnancy among three beef cattle genetic groups using field data. Vaginal swabs were collected from 74 cows at Fort Keogh, MT, including 23 Angus, 23 Hereford Line 1, and 28 crossbreds, and DNA was extracted and analyzed via 16S rRNA gene amplification. Significant differences in alpha diversity (p < 0.05) were found among Line 1 cows compared to Angus and crossbreds in many indicators of alpha diversity. Pregnancy status did not influence alpha diversity of samples significantly, but trends toward significance were observed. PERMANOVA analysis indicated that genetic groups and pregnancy status affected microbial composition (p < 0.05), but their interaction was not significant. Each genetic group showed unique compositions of operational taxonomic units (OTUs), with higher proportions of Ureaplasma and Mycoplasma families in Line 1. Additionally, variations in microbial communities were observed between pregnant and non-pregnant cows, with certain uncultured bacteria more prevalent in non-pregnant cows. While field data are useful for such studies and represent a real production system, better-designed experiments are necessary to validate findings and test hypotheses. These results suggest variation in vaginal microbiomes across breeds and pregnancy status, emphasizing the need for further research to identify factors affecting these changes. Full article

Water scarcity, poor soil, and low water and fertilizer utilization are major challenges on agricultural production in the tableland region of China’s Loess Plateau. Optimizing tillage patterns and improving soil nutrient status can improve crop yield and water and fertilizer utilization efficiency. A field trial was initiated in 2005 to assess the impacts of various tillage and fertilization practices on dryland agricultural production. A split-plot design was used, with tillage practices (traditional tillage and no tillage) as the main plot treatment and fertilization management (no fertilization (CK), mineral nitrogen (N), mineral phosphorus (P), composted cow manure (M), a combination of mineral nitrogen and phosphorus (NP), and a combination of mineral nitrogen, phosphorus, and composted cow manure (NMP)) as the split-plot treatment. An experiment was conducted from 2022 to 2024. The NMP treatment resulted in lower bulk density, a lower three-soil-phase index, and higher mean weight diameter, geometric mean diameter, soil water storage, total nitrogen, and soil organic matter than the CK. In the no-tillage treatment, the crop roots were less effective at extracting water from the deep subsoil, leading to greater residual moisture at depth (especially in the 120–200 cm soil layer) and lower yield and water use efficiency than in traditional tillage. The grain yield and water use efficiency were 9.2% and 8.4% lower, respectively, under no tillage than under traditional tillage. The NMP under traditional tillage exhibited lower surface soil bulk density and a higher three-soil-phase index, mean weight diameter, geometric mean diameter, soil organic matter, total nitrogen, and water use efficiency than the unfertilized control, resulting in higher grain yields. The NMP under traditional tillage is recommended to increase grain yield and water use efficiency in wheat–maize rotation systems in the tableland region of China’s Loess Plateau. Future studies should analyze the deep root architecture and the effect of weed competition on soil water depletion. Full article

The objective of this study was to evaluate the impact of summer calving on reproductive performance, milk yield, and culling risk in U.S. organic dairy herds. Data were obtained from the Dairy Herd Improvement records of 434 organic dairy herds across 31 states, comprising 287,927 calving records from 2018 to 2022. Calving months were categorized as winter, spring, summer, or fall. Mixed models were used to analyze the 305 d milk yield, 305 d energy-corrected milk yield, peak milk DIM, peak test-day milk yield and logistic regression, and survival analyseswere applied to analyze calving difficulty, abortion, pregnancy diagnosis, and culling outcomes. Cows calving during summer had fewer days to first service and fewer days open compared with cows calving in other seasons; however, their odds of being diagnosed as pregnant were significantly lower. Summer-calving cows produced lower 305 d milk and energy-corrected milk yields, reached peak milk production later, and had a lower peak test-day milk yield. Calving during summer was also associated with increased odds of calving difficulty and a higher risk of culling. These findings indicate that summer calving poses distinct challenges for organic dairy cows, with cumulative effects on fertility, milk production, and survivability, underscoring the need for season-specific management strategies in organic dairy systems. Full article

The CSN2 gene encoding β-casein has gained increasing attention in dairy cattle breeding due to the global adoption of A2-oriented selection strategies. However, robust large-scale evidence assessing potential unintended effects on functional traits, particularly fertility, under intensive commercial conditions remains limited. This study evaluated whether selection for the CSN2 A2 β-casein variant is associated with biologically relevant differences in fertility traits in Holstein cows. Reproductive and genomic data from 7826 lactation records of 2773 Holstein cows collected between 2022 and 2025 in a large commercial dairy herd were analyzed. Fertility indicators included days open, number of services per conception, calving interval, first-service conception rate, and pregnancy by 100 days in milk. Mixed-effects models accounting for repeated lactations and cow- and sire-level clustering were applied, and predefined equivalence margins were used to distinguish statistical non-significance from biological irrelevance. Across all evaluated fertility traits, differences among CSN2 genotypes (A1A1, A1A2, and A2A2) were consistently small, biologically negligible, and well within predefined equivalence margins. Differences in days open were within ±2 days, and effect sizes for count and binary traits were close to unity. Parity and calving year significantly influenced reproductive performance, whereas no CSN2 genotype × parity interactions were detected. These findings indicate that selection for the CSN2 A2 β-casein variant does not compromise reproductive performance under intensive commercial management conditions. From a breeding and industry perspective, the results support the implementation of A2-oriented selection strategies without biologically meaningful adverse effects on fertility. Full article

The overreliance on non-renewable phosphate fertilizers necessitates sustainable alternatives for phosphorus recycling in agriculture. This study aimed to characterize and enhance the metabolic activity of phosphate-solubilizing microorganisms isolated from compost by eliciting them with two distinct mesoporous silica nanoparticles: standard SBA-15-S and short-pore SBA-15-C. Bacterial strains with broad-spectrum P solubilization and mineralization capacities were isolated from the mesophilic phases of tomato greenhouse and cow manure composts. These isolates received treatment with nanoparticle concentrations of 0.1, 10, and 100 ppm. The results demonstrated that nanoparticle elicitation significantly altered microbial growth, solubilization halos on tricalcium phosphate, and the specific activity of acid, neutral, and alkaline phosphatases in a strain- and nanoparticle-dependent manner. Notably, SBA-15-C at 100 ppm consistently enhanced multiple P-recycling properties across several strains, including Proteus and Myroides species. Principal component analysis revealed distinct behavioral clusters between composting phases and isolation methods. The findings indicate that tailored silicon oxide nanostructures can serve as eustressors to modulate and enhance the P-solubilizing and mineralizing functions of compost-derived microbiota, offering a promising nanobiostimulation strategy for developing enhanced biofertilizers. Full article

Under binding resource constraints and rising environmental pressures, enabling poor smallholders to raise incomes while reducing chemical input use remains a key policy challenge in many developing economies. Drawing on two waves of household survey data from Guangxi, China, this study evaluates the impacts of small-scale beef cattle rearing promoted through an incentive-based subsidy scheme (Yijiang Daibu). Exploiting quasi-experimental variation in household participation, we combine Coarsened Exact Matching (CEM) with household fixed-effects models to address selection bias and time-invariant unobserved heterogeneity. The results show that participation in dispersed cattle rearing significantly increases household welfare (measured by per capita consumption expenditure) while simultaneously reducing chemical fertilizer application, indicating clear economic and environmental co-benefits. Mechanism evidence suggests that substituting cattle manure for chemical fertilizer plays a central role in driving fertilizer reduction. Moreover, access to credit, participation in farmer cooperatives, and internet access further reinforce both welfare gains and manure–fertilizer substitution effects. Overall, incentive-based support for small-scale livestock production, when aligned with local resource endowments, can deliver both welfare gains and greener production outcomes. This study provides micro-level evidence on how well-designed industrial support policies can jointly promote inclusive growth and agricultural sustainability in smallholder-dominated regions. Full article

This study analyzed how climate variability affects lactation yield and reproduction in Holstein cows in a continental climate. It specifically compared Wet-Bulb Temperature (T_wb) with the standard Temperature–Humidity Index (THI). We conducted a retrospective study on a dairy farm in Konya, Türkiye, analyzing a total of 144 complete lactation records from a herd of 90 cows calving between 2022 and 2023. To rule out nutritional factors, a consistent TMR diet without pasture access was maintained in both years. Average Daily Milk Yield (ADMY) was calculated to adjust for lactation length. Climatic data showed a distinct contrast. Ambient temperatures and THI were similar between years (p > 0.05). However, 2022 was characterized by “humid heat” (high T_wb), while 2023 presented a “dry heat” profile with significantly lower T_wb (p < 0.001). This difference significantly impacted performance. Cows in the 2023 group produced much higher milk yields (50.55 ± 3.01 kg) than the 2022 group (30.74 ± 0.81 kg) (p < 0.001). Unexpectedly, milk yield peaked during the Autumn and Summer seasons of the low-humidity year. In contrast, fertility declined with thermal load. Poor winter fertility suggested a “carry-over” effect of previous heat stress. These findings show that T_wb is a better indicator of thermal comfort than THI in continental climates. Furthermore, low humidity can significantly reduce the negative impact of heat on milk production. Full article

Reduced winter hair shedding in beef cows through the spring and summer months may contribute to heat stress and reduced performance in spring-calving herds. This study evaluated the relationship of hair shedding with the fertility and maternal performance of 72 Dexter cows. Hair shedding data for 20 May, 3 June, 17 June, and 1 July in 2019 were used to classify cows as high or low hair shedders. Hair shedding levels were lower (p < 0.05) for 2-year-old cows than for cows 7+ years of age for the first three dates and lower (p ≤ 0.05) for lactating cows than for dry cows on the first two dates. Concurrent and four years of historical performance records were used to assess the associations between hair shedding and cow–calf performance. Data from 230 natural matings in July and August from 2015 to 2019 were analyzed. Birth to weaning weight data were recorded from 2016 to 2019 on 124 spring-born calves. Cow fertility was higher (p < 0.05) for high-shed cows than for low-shed cows for the 1 July classification. When the records from cows that were dry in 2019 were excluded from testing, fertility was higher (p < 0.05) for high-shed cows than for low-shed cows at all four scoring dates. The associations of cow hair shedding levels with preweaning calf performance were minimal. Dexter cows exhibiting higher hair shedding levels in the spring and summer expressed higher summer fertility. Full article

Cryopreservation of bovine ovarian cortical tissue offers a promising strategy for preserving female fertility and genetic resources, yet outcomes remain variable and influenced by both protocol and tissue size. This study investigated how slow freezing-thawing (SFT) and two vitrification-warming procedures (VW1 and VW2) affect preantral follicle morphology and granulosa cell proliferation in bovine ovarian cortex fragments of two dimensions (1 × 10 × 5 mm and 1 × 10 × 10 mm). Tissue from six cows was processed for histological evaluation and Ki67 immunostaining. Small fragments subjected to SFT showed no significant reduction in the proportion of morphologically normal follicles compared with fresh controls, representing the best overall preservation. In contrast, vitrification decreased morphological integrity, with VW2 performing better than VW1 in both fragment sizes. Small SFT pieces contained more morphologically normal follicles than large ones. Granulosa cell proliferation capacity was largely maintained across cryopreservation protocols, increasing with follicular stage; a size-related difference only appeared on VW2, where small fragments displayed higher Ki67 positivity. These findings underscore the relevance of jointly evaluating cryopreservation protocol and fragment size to optimize bovine ovarian tissue preservation, strengthening the evidence supporting SFT of small fragments as a robust option for safeguarding cortical integrity and improving tissue-based fertility preservation strategies. Full article

Overuse of synthetic pesticides and fertilizers has increased concerns regarding environmental and human health. Indian natural farming practices, which are mainly based on different bioformulations, provide sustainable alternatives to conventional farming. Among other bioformulations, Beejamrit is a cow-based biostimulant that is used for seed treatment to promote seed germination, seed vigor, and tolerance to pathogens. In this study, 16S rRNA amplicon metagenomics and untargeted metabolomics (GC-MS and LC-MS) approaches were employed to evaluate microbial and metabolic profiles of Beejamrit samples, respectively. Metagenomic analysis indicated that Beejamrit consisted of different plant-growth-promoting bacteria, such as Advenella, Comamonas, Lysinibacillus, Acinetobacter, and Arcobacter. GC-MS analysis discovered organoheterocyclics (23%) to be the most prevalent metabolite group in Beejamrit, followed by organic acids (18%) and benzenoids (15%). In LC-MS analysis, lipids (26%) were most abundant, followed by organoheterocyclics (18%) and organic acids (18%). Furthermore, GC-MS and LC-MS analyses identified a wide range of metabolites, including amino acids, organic acids, phenolics, and fatty acids. These findings confirm that Beejamrit contains a wide array of beneficial bacteria and bioactive compounds, thereby elucidating the potential mechanisms behind its efficacy as an effective seed treatment agent. The study offers an initial framework for further standardization and wider application in sustainable agriculture. Full article

The success rate of embryo transfer (ET) is influenced by various factors, including embryo quality, environmental conditions, and recipient cows. This study examines the impact of ET on embryo success rates using embryos produced using the ovum pickup method, with fresh and frozen embryos generated on day 7 after in vitro fertilization and subsequently implanted into recipient cows. The factors that contributed to the success rate of ET were investigated, with a focus on embryo type, parity, and farm management. Furthermore, metabolic profile tests were conducted to determine the factors underlying the observed differences in pregnancy rates across the farms. The analysis revealed that pregnancy rates did not significantly differ according to embryo type or parity. However, significant differences (p < 0.05) were observed in the glucose, cholesterol, non-esterified fatty acid, total protein, globulin, albumin/globulin, and aspartate aminotransferase levels of the recipients. Furthermore, a comparison of farms with high and low pregnancy rates revealed significant differences in the glucose, cholesterol, total protein, albumin, globulin, and albumin/globulin levels (p < 0.05). Differences between farms were associated with the feed management strategy, which highlights the significance of optimal recipient cow management. Full article

Hyperketonemia (HYK), defined by blood beta-hydroxybutyrate (BHB) ≥ 1.2 mmol/L, is described as a significant risk factor for cows developing postpartum (pp) diseases and impaired reproductive performance. The goal of the present study was to observe metabolic challenges in transition cows and to identify systemic markers reflecting HYK associated with lessened reproductivity. Fifty-four Simmental cows were monitored, revealing approximately 30% prevalence of HYK at the early pp period on 7, 14, or 28 days in milk (DIM). We assessed the dry matter intake, rumination time (RT), serum liver activity index, non-esterified fatty acids (NEFAs), acute phase proteins, and uterine and oviductal health. Elevated NEFA and reduced RT 14 days antepartum were a good predictor for HYK at 7 DIM. Hyperketonemia at 14 DIM resulted in higher milk yield compared with controls. We could neither detect differences in uterine health nor in reproductive key performance parameters between hyperketonemic and control cows, whereby the proportion of polymorphonuclear neutrophils in oviductal epithelia was significantly lower in hyperketonemic cows 14 DIM. We conclude that elevated concentrations of BHB in HYK 7, 14, or 28 DIM indicated energy supply to support physiological metabolic adaptations and lactation and that, in the absence of excessive inflammation during the transition period, HYK was not a risk factor for impaired fertility. Full article

Combined application of organic (M) and chemical fertilizer (C) is a significant measure to enhance soil fertility and ensure food security. In 2023 and 2024, we established six treatments: T1 (no fertilization), T2 (100% C), T3 (75% C + 25% M), T4 (50% C + 50% M), T5 (25% C +75% M), and T6 (100% M), with three replicates for each treatment. The total amount of nitrogen applied to the soil for T2–T6 was the same, and the organic fertilizer was compost sourced from cow dung. The aims of this study were to explore the effects of organic fertilizer combined with chemical fertilizer on soil fertility, and apparent nutrient balance, to investigate its possible economic benefits. We also analyzed the influence of the combined application of organic and chemical fertilizers on the degree of coupling and coordination (D) between soil fertility and economic benefits. The total phosphorus, total potassium, available phosphorus, available potassium, and organic matter in the soil all showed an increasing trend with an increase in the proportion of organic fertilizer applied. T2 reduced the soil pH by 7.41–8.94% compared with T1, while applying organic fertilizers (T3–T6) increased the soil pH by 0.72–8.62% compared with T2. T4 is conducive to the balance of income and expenditure of nitrogen, phosphorus, and potassium elements. The corn yield, net income, and input–output ratio all showed an initial increase followed by a decrease with an increase in the proportion of organic fertilizer applied, and their values all reached the maximum under T4. Based on the CRITIC-TOPSIS method and the coupling coordination degree model, it was determined that the fertilization strategy with the highest comprehensive score and D under the conditions of this experiment was 50% C +50% M (T4), which not only improved soil fertility but also achieved the highest economic benefit. The research results were of great significance for promoting sustainable agricultural development. Full article

The application of organic fertilizer is an effective way to improve soil fertility and promote seedling growth. Toona fargesii (T. fargesii) is a fast-growing tree with high commercial value due to its excellent timber quality. However, the mechanism underlying its rapid growth at the seedling stage in red soil remains unclear. Here, we investigated the effects of cow manure application (OF group) on soil nutrients and rhizosphere microbial communities in red soil, as well as how it promotes the seedling growth of T. fargesii. Seedlings in the OF group showed a significantly higher specific growth rate than those in the unfertilized control (CK) group (73.51 ± 11.82% vs. 34.90 ± 5.49%, p = 0.022). This growth promotion was accompanied by an increase in soil pH (6.36 ± 0.01 vs. 6.22 ± 0.02, p = 0.001) and a concurrent decrease in NO₃⁻-N (10.60 ± 3.20 vs. 22.58 ± 3.49, p = 0.044). High-throughput sequencing demonstrated that cow manure tended to enhance bacterial diversity while decreasing fungal diversity. The OF treatment significantly enriched the relative abundances of bacterial phyla Myxococcota, Bacteroidota, Firmicutes, and Proteobacteria, while reducing Chloroflexi. For fungi, the relative abundances of Calcarisporiellomycota and Chytridiomycota were reduced under OF treatment. Redundancy analysis indicated that soil pH and organic matter (OM) content were the main environmental drivers shaping rhizosphere microbial communities. Our results demonstrated that short-term cow manure application raised soil pH and shifted the microbiome, coinciding with promoted seedling growth. This study provides insights into the microbiome-mediated rapid growth of tree seedlings in red soil. It implies that applying cow manure is an effective way to promote seedling performance in the early stages. Full article

Manure management costs are a substantial component of overall costs on a modern dairy farm. Due to the slim margins of contemporary milk production operations, dairies are under constant pressure to increase milking herd sizes to take advantage of size economies that enable them to compete in the global marketplace. This study provides a current assessment of manure management and overall costs and returns on four standard sizes of dairies typical of the southern Great Plains, particularly north central Texas. The study is necessitated by the fact that the changing economic landscape has resulted in substantial changes in manure management practices. This study also forms the basis for additional analyses that will explore alternative value-added options for dairy manure management. We utilize the Farm-level Economic Model to holistically simulate the costs and returns of four representative dairy herd sizes—small (300 cows), medium (720 cows), large (1500 cows), and very large (5000 cows). Based on prevailing assumptions about land areas farmed and farm management practices, we find that dairy farms require between 0.18 and 0.4 ha/cow to manage manure based on crop nitrogen uptake rates, versus 0.67 to 0.95 ha/cow for crop phosphorus uptake rates. Manure application costs alone range from USD 55/cow (USD 225/ha) to USD 115/cow (USD 300/ha) depending on dairy size, but some of these costs are offset by fertilizer cost savings. Proportion of manure hauled offsite ranges from 9% to 67% for phosphorus-based applications, depending on herd size, and net incomes per cow are reduced by USD 60 to USD 100 (USD 4.33 to USD 8.27 per Mg of milk) if manure is applied based on phosphorus uptake rates as compared to nitrogen uptake rates of receiving crops. Generating a broad array of economically viable value-added product options from dairy manure would enable farmers to be more competitive in a market characterized by thin margins. Full article