Search Results (9,587)

Cultivated land quality is critical for soil productivity and scientific fertilization. This study analyzed its evolution and impact on soil productivity across three ecological regions (southern, central, and northern Shanxi) in Shanxi Province, China, from 1998 to 2021). Using data from 8 long-term experimental sites (1998–2021) and 50 monitoring stations (2016–2021), we employed random forest analysis to evaluate temporal trends in key soil indicators. The results show the following: (1) Northern Shanxi exhibited the greatest improvement in soil fertility, with organic matter increasing by 98.2%, total nitrogen by 57.2%, available phosphorus by 131.7%, and available potassium by 17.1%. (2) Nitrogen fertilizer application increased across all regions, while phosphorus and potassium inputs generally declined. (3) Crop yields improved substantially—southern Shanxi wheat and maize increased by 15.3% and 20.9%, respectively, while central and northern Shanxi maize yields rose by 30.9% and 75.4%. Random forest models identified regional characteristics (40%), nitrogen fertilization (20%), and available phosphorus (18%) as primary influencing factors. Although cultivated land quality improved overall, soil fertility remained medium to low. Region-specific management strategies are recommended: rational nitrogen use in all regions; nitrogen control with phosphorus supplementation in the south; focused improvement of available phosphorus and potassium in the center; and increased organic fertilizer in the north. These measures support scientific nutrient management and sustainable agricultural production. Full article

Long-term greenhouse operations face a critical challenge in the form of soil quality degradation, yet the key intervention periods and underlying mechanisms of this process remain unclear. This study aims to quantify the effects of greenhouse lifespan on the evolution of soil quality and to identify critical periods for intervention. We conducted a systematic survey of greenhouse operations in a representative area of Yunnan Province, Southwest China, and adopted a space-for-time substitution design. Using open-field cultivation (OF) as the control, we sampled and analyzed soils from vegetable greenhouses with greenhouse lifespans of 2 years (G2), 5 years (G5), and 10 years (G10). The results showed that early-stage greenhouse operation (G2) significantly increased soil temperature (ST) by 8.38–19.93% and soil porosity (SP) by 16.21–56.26%, promoted nutrient accumulation and enhanced aggregate stability compared to OF. However, as the greenhouse lifespan increased, the soil aggregates gradually disintegrated, particle-size distribution shifted toward finer clay fractions, and pH changed from neutral to slightly alkaline, exacerbating nutrient imbalances. Compared with G2, G10 exhibited reductions in mean weight diameter (MWD) and soil organic matter (SOM) of 2.41–5.93% and 24.78–30.93%, respectively. Among greenhouses with different lifespans, G2 had the highest soil quality index (SQI), which declined significantly with extended operation; at depths of 0–20 cm and 20–40 cm, the SQI of G10 was 32.59% and 38.97% lower than that of G2, respectively (p < 0.05). Structural equation modeling (SEM) and random forest analysis indicated that the improvement in SQI during the early stage of greenhouse use was primarily attributed to the optimization of soil hydrothermal characteristics and pore structure. Notably, the 2–5 years was the critical stage of rapid decline in SQI, during which intensive water and fertilizer inputs reduced the explanatory power of soil nutrients for SQI. Under long-term continuous cropping, the reduction in MWD and SOM was the main reason for the decline in SQI. This study contributes to targeted soil management during the critical period for sustainable production of protected vegetables in southern China. Full article

Nitrogen (N) and phosphorus (P) play vital roles in crop growth. However, conventional fertilizers exhibit low utilization efficiency, making them prone to causing resource wastage and water eutrophication. Although metal–organic frameworks (MOFs) have shown great potential for application in controlled-release fertilizers (CRFs), currently reported MOF-based CRFs suffer from low nutrient content, which limits their further application. To address this issue, this study synthesized a series of hierarchically porous MOFs, denoted as MIL-156(X), using sodium acetate as a modulator under hydrothermal conditions. These materials were subsequently loaded with urea and phosphate from aqueous solution to form MOFs-based CRFs (N-P-MIL-156(X)). Results indicate that MIL-156(X) retain microporous integrity while incorporating abundant mesopores. Increasing modulator content reduced particle size and average pore diameter but increased specific surface area and adsorption capacity for urea and phosphate. MIL-156-H (with a high modulator content addition) exhibited the highest adsorption capacity, conforming to Langmuir isotherm and pseudo-second-order kinetics. The adsorption mechanisms of urea and phosphate involved hydrogen bonding and the formation of Ca intra-spherical complexes, respectively. N-P-MIL-156-H contained 10.8% N and 16.3% P₂O₅, with sustained release durations exceeding 42 days (N) and 56 days (P₂O₅) in an aqueous solution. Pot trials demonstrated significantly higher nutrient use efficiency (N-44.8%, P₂O₅-16.56%) and a 12.22% yield increase compared to conventional fertilization (N-35.6%, P₂O₅-13.32%). Thus, N-P-MIL-156-H-based fertilization significantly promotes rice growth and N/P utilization efficiency, offering a promising strategy for developing controlled-release fertilizers and improving nutrient management. Full article

Cover crops and mulches are widely used techniques for limiting weeds and pests’ effects on crops. This study compared six practices over two growing seasons in two organic farms in Cuneo province, North-West Italy: two bio-based biodegradable mulch sheets (BM01 and BM02), dead mulch (hazelnut shells), living mulch (Trifolium repens L.), mechanical control, and an untreated control. Spring crops included Lactuca sativa L. var. capitata, Allium cepa L. cv. ‘Tropea’, and Brassica oleracea L. var. italica, while autumn crops were Lactuca sativa L. var. capitata, Allium fistulosum L., and Brassica oleracea L. var. italica. Weed infestation was evaluated through density (n/m²), biomass (g/m²), and diversity (Shannon Index), alongside crop yield and quality. Biodegradable mulch sheets provided the greatest weed suppression, followed by hazelnut shells, while living mulch and untreated control showed the highest weed pressure. Crop yield varied significantly among practices and species: BM01 and BM02 resulted in the highest yields, while living mulch consistently produced the lowest. Lettuce displayed the best quality across both farms, whereas onion quality varied by site. The highest quality scores were observed under biodegradable mulches and mechanical control, while living mulch and untreated control yielded the poorest results. Overall, biodegradable mulches emerged as the most effective balance between weed suppression, crop yield, and quality in organic systems. Full article

Pre-harvest sprouting (PHS) reduces the quality and quantity of crop seeds. PHS can be imposed through the embryo or husk pathway of cereal crops. Most reported PHS seeds are imposed via the embryo pathway. Here, we generated transgenic rice plants overexpressing rice melatonin 2-hydroxylase (OsM2H), which catalyzes the hydroxylation of melatonin to 2-hydroxymelatonin (2-OHM). OsM2H overexpression (M2H-OE) showed PHS under paddy conditions. Germination assays revealed that intact seeds harvested at 26 and 36 days after heading (DAH) showed PHS, whereas dehusked seeds did not, indicating husk-imposed PHS. Overproduction of 2-OHM was observed in M2H-OE seeds compared to wild-type control. In addition, M2H-OE lines produced more hydrogen peroxide than the wild-type. 2-OHM-induced reactive oxygen species resulted in the induction of OsGA3ox2, a gibberellin (GA) biosynthesis gene, and repression of OsGA2ox3, a GA degradation gene, in caryopses at 2 DAH, but in the induction of the ABA degradation gene OsABA8ox3 in intact seeds at 26 DAH. In addition, M2H-OE seedlings were longer and showed increased levels of hydrogen peroxide and OsGA3ox2 expression versus the wild-type. This is the first report showing that 2-OHM can induce PHS via the husk pathway in rice seeds through the induction of GA biosynthetic and ABA degradation genes. Full article

Atractylodes macrocephala Koidz. (A. macrocephala), a medicinal plant extensively used in traditional Chinese medicine, is greatly susceptible to root rot under continuous monoculture, leading to serious yield and quality losses. To develop a sustainable control strategy, we isolated the endophytic bacterium Bacillus velezensis (B. velezensis) Amzn015 from healthy A. macrocephala plants and assessed its biocontrol efficacy and underlying mechanisms. In vitro assays showed that Amzn015 significantly inhibited Fusarium oxysporum and other phytopathogenic fungi by disrupting hyphal morphology and reducing spore viability. Pot experiments confirmed its effectiveness in reducing disease incidence and promoting plant growth. Mechanistically, Amzn015 induced reactive oxygen species accumulation and upregulated key defense responsive genes involved in salicylic acid, jasmonic acid/ethylene, and phenylpropanoid signaling pathways. The findings imply that Amzn015 synchronously activates systemic acquired resistance and induced systemic resistance in A. macrocephala. This dual activation contributes to enhanced immunity and plant vigor under pathogen challenge. Our findings offer fresh perspectives on the biocontrol potential of endophytic B. velezensis Amzn015 and support its application as an eco-friendly agent for managing root rot in medicinal crops. Full article

The cowpea weevil, Callosobruchus maculatus (F.), stands out as one of the most destructive field-to-storage pests of leguminous crops. This study investigates the potential of essential oils derived from two Satureja species, Satureja hortensis L. and Satureja khuzistanica Jamzad, for managing C. maculatus. Bioassay results revealed that both S. hortensis (72 h LC₅₀ = 0.20 µL/g) and S. khuzistanica (72 h LC₅₀ = 0.19 µL/g) essential oils exhibited significant toxicity against C. maculatus adults. The essential oils extended development time, reduced adult longevity, and decreased fecundity of the pest. Key population parameters, including intrinsic growth rate (r) and net reproductive rate (R₀), were significantly lowered, particularly by S. hortensis essential oil. Age-specific survival (l_x) and fecundity (m_x) rates were also declined in treated groups, with delayed reproductive peaks compared to controls. Chemical analyses of S. hortensis and S. khuzistanica essential oils indicated that carvacrol (30.9% and 62.9%, respectively), γ-terpinene (25.5% and 4.3%), p-cymene (9.7% and 7.9%), and thymol (3.7% and 9.3%) were the major components. Hierarchical cluster analysis (HCA) was carried out to compare and contrast the compositions with previous works. The results demonstrated that S. hortensis and S. khuzistanica essential oils, given their lethal and sublethal effects against C. maculatus, can be introduced as natural alternatives to hazardous chemical insecticides, highlighting the need for further research in this field. Full article

Light-dependent protochlorophyllide oxidoreductase (LPOR, E.C.1.3.1.33) plays a crucial role in the biosynthesis of chlorophyll in plants. Therefore, inactivating LPOR can hinder the production of chlorophyll to achieve the effect of weed control. In this research, utilizing an active substructure splicing method, 20 new 1,2,4-oxadiazole compounds targeting LPOR were synthesized. Among them, compounds 5j, 5k and 5q exhibited superior inhibitory efficacy in greenhouse herbicidal trials. In vitro enzyme activity assays indicated that 5q significantly inhibited Arabidopsis thaliana LPOR (AtLPOR), with an IC₅₀ value of 17.63 μM. Furthermore, compound 5q exhibited superior crop safety and holds potential application prospects for weed management in cotton. Molecular docking and dynamic simulations were employed to elucidate the binding mode and molecular mechanism of 5q with AtLPOR. These experimental and theoretical results indicate that 5q is a promising candidate for the development of novel herbicides targeting LPOR. Full article

Vineyard cover crops deliver well-documented ecosystem services, yet consistent establishment, especially of perennial grasses and legumes, remains a primary barrier to adoption. This review reframes “companion (nurse) cropping” not as a new crop class but as a acilitative establishment strategy within the broader cover-/service-crop literature. We (i) position our contribution relative to recent syntheses, (ii) synthesize evidence on companion crops practices that reduce cover cropping early failure risk, and (iii) propose a testable research agenda. A focused scoping review of peer-reviewed and extension literature indexed in Web of Science and Google Scholar was conducted using search terms encompassing cover/service crops and nurse/companion/facilitation in viticulture systems. Across climates, fast-establishing cereals (Avena sativa, Hordeum vulgare, Secale cereale, × Triticosecale Wittmack) and short-cycle legumes (Vicia sativa, Pisum sativum, Trifolium incarnatum) can reliably “nurse” slower perennials and legumes by providing early groundcover, weeds control, and microclimate buffering when sown at reduced rates (≈25–50% of monoculture) and terminated on time to limit vine competition. Evidence gaps persist for in-row applications, water-use penalties under drought, and long-term effects on yield and grape composition. Companion cropping is argued to be a design principle in vineyard cover-crop programs rather than a separate category. A decision framework and research agenda are presented to quantify establishment reliability, resource trade-offs, and wine-relevant outcomes, and it is recommended that future decision tools make the companion-phase logic explicit to de-risk adoption and align with regional guidelines. Full article

The identification of cotton diseases and pests is crucial for maintaining cotton yield and quality. However, conventional manual methods are inefficient and prone to high error rates, limiting their practicality in real-world agricultural scenarios. Furthermore, Convolutional Neural Network–Long Short-Term Memory (CNN-LSTM) models are insufficient in generating fine-grained and semantically rich image captions, particularly for complex disease and pest features. To overcome these challenges, we introduce CottonCapT6, a novel multi-task image captioning framework based on the Cross Vision Transformer (CrossViT-18-Dagger-408) and Text-to-Text Transfer Transformer (T5). We also construct a new dataset containing annotated images of seven common cotton diseases and pests to support this work. Experimental results demonstrate that CottonCapT6 achieves a Consensus-based Image Captioning Evaluation (CIDEr) score of 197.2% on the captioning task, demonstrating outstanding performance. Notably, the framework excels in providing more descriptive, coherent, and contextually accurate captions. This approach has strong potential to be deployed in cotton farms in the future, helping pest control personnel and farmers make precise judgments on cotton diseases and pests. However, its generalizability to other crops and environmental conditions remains an area for future exploration. Full article

Insects use chemical compounds to communicate with conspecifics and other organisms. The corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae) (DeLong & Wolcott), is an important pest in Brazilian maize crops due to its role as a vector of phytopathogens. Despite its economic importance, the chemical communication between sexes in this species remains to be elucidated. This research aimed to unveil whether D. maidis produces chemical compounds that influence the behavior of the opposite sex and may act as sex pheromones. To evaluate the influence of these volatiles, olfactometer bioassays were conducted as dynamic headspace volatile collections from live insects. Results showed that both male and female leafhoppers emit volatile compounds; however, no sex-specific compounds were detected. Females were attracted to male odors and male aeration extracts, suggesting males produce sex-specific volatiles. Interestingly, males avoided odors from non-acclimated females, which may indicate possible alarm pheromone release. Although the compounds were not identified, this is the first study to demonstrate intraspecific chemical communication in D. maidis mediated by volatiles, and the first such record in Membracoidea. These results contribute to understanding the pest’s biology and support the development of monitoring and control strategies in maize crops. Full article

The growing potassium (K) demand and supply–demand imbalance in intensive agriculture require the development of multi-nutrient K fertilizers. Polyhalite (POLY), a multi-nutrient natural mineral rich in K, calcium, magnesium, and sulfur, can enhance soil nutrient diversity and fertility. However, research on its synergistic application with nitrogen (N) fertilizer remains limited. Therefore, this study was designed to apply three different fertilizer composites at four N concentration gradients through field plot experiments to evaluate crop productivity and nutrient use efficiency. Results revealed that the application of both compound fertilizers with N fertilizer increased maize yield, ranging from 1.03% to 11.53%, compared with the PK control. Moreover, 25-7-8 (MOP)(POLY26%) achieved a maximum yield of 9499.88 kg/ha at the N1 (170 kg/ha) level. This represents a significant increase of 11.53% compared with the PK control. Moreover, the application of compound fertilizer containing POLY could significantly increase the N fertilizer utilization rate; improve the quality of maize; and exert a significant effect on soil pH, EC, and nutrient content. This study paves the way for broader application of POLY by establishing its novel role as a sustainable nutrient source. It provides critical strategic guidance for advancing global resource-efficient agriculture. Full article

Global food production must meet the dietary requirements of a growing population, which is expected to reach 8–11 billion by 2100, while reducing the environmental impact of agricultural practices. The agricultural sector accounts for 21–37% of global greenhouse gas emissions, 70% of freshwater, and contributes considerably to biodiversity loss and challenges that are further intensified by climate change. Controlled Environment Agriculture (CEA) serves as a sustainable strategy to address global food production and promote consistency and resource-efficient crop production. However, nutrient imbalances remain a key challenge in hydroponic CEA systems. To address these nutrient-related challenges, plant sap analysis is being considered as real-time monitoring tool and precise nutrient management in CEA systems. Compared to traditional nutrient tissue analysis, sap analysis shows stronger correlations with crop performance during active growth. For instance, petiole sap nitrate-nitrogen (NO₃⁻-N) and total nitrogen (N) in tomato leaves show correlation coefficients of r = 0.6–0.8 during their rapid vegetative growth stages. Sap analysis shows potential improvements in nutrient efficiency, crop quality, and sustainability within CEA. This review investigates the principles, methodologies, and advancements in plant sap analysis, contrasting it with traditional nutrient testing methods. It also addresses challenges such as variability in sap composition, the lack of standardized protocols, and economic considerations, while emphasizing real-time nutrient management to achieve and sustainability in CEA. Full article

The cereal cyst nematode, Heterodera avena, is responsible for substantial economic losses in the global production of wheat, barley, and other cereal crops. Extracellular enzymes, particularly those from the glycoside hydrolase 18 (GH18) family, such as chitinases secreted by Trichoderma spp., play a crucial role in nematode control. However, the genome-wide analysis of Trichoderma longibrachiatum T6 (T6) GH18 family genes in controlling of H. avenae remains unexplored. Through phylogenetic analysis and bioinformatics tools, we identified and conducted a detailed analysis of 18 GH18 genes distributed across 13 chromosomes. The analysis encompassed gene structure, evolutionary development, protein characteristics, and gene expression profiles following T6 parasitism on H. avenae, as determined by RT-qPCR. Our results indicate that 18 GH18 members in T6 were clustered into three major groups (A, B, and C), which comprise seven subgroups. Each subgroup exhibits highly conserved catalytic domains, motifs, and gene structures, while the cis-acting elements demonstrate extensive responsiveness to hormones, stress-related signals, and light. These members are significantly enriched in the chitin catabolic process, extracellular region, and chitinase activity (GO functional enrichment), and they are involved in amino sugar and nucleotide sugar metabolism (KEGG pathway enrichment). Additionally, 13 members formed an interaction network, enhancing chitin degradation efficiency through synergistic effects. Interestingly, 18 members of the GH18 family genes were expressed after T6 parasitism on H. avenae cysts. Notably, GH18-3 (Group B) and GH18-16 (Group A) were significantly upregulated, with average increases of 3.21-fold and 3.10-fold, respectively, from 12 to 96 h after parasitism while compared to the control group. Meanwhile, we found that the GH18-3 and GH18-16 proteins exhibit the highest homology with key enzymes responsible for antifungal activity in T. harzianum, demonstrating dual biocontrol potential in both antifungal activity and nematode control. Overall, these results indicate that the GH18 family has undergone functional diversification during evolution, with each member assuming specific biological roles in T6 effect on nematodes. This study provides a theoretical foundation for identifying novel nematicidal genes from T6 and cultivating highly efficient biocontrol strains through transgenic engineering, which holds significant practical implications for advancing the biocontrol of plant-parasitic nematodes (PPNs). Full article

Wheat (Triticum aestivum L.) is a vital food crop for majority of the world’s population. However, its yield potential is significantly threatened by insect pests, which adversely affect production, quality, and overall food security. The diverse array of insect pests throughout wheat’s growth stages necessitates a comprehensive understanding of their interactions with wheat cultivation. This review critically assesses the diversity, biology, ecology, and management strategies of major insect pests in North India, including aphids, termites, pink stem borer, gram pod borer, armyworm, and brown wheat mite. These pests infest wheat at various growth stages, posing significant challenges to sustainable production. Moreover, existing pest control strategies are challenged by evolving agronomic practices in the region and climate change globally. As agricultural systems worldwide aim for sustainability and resilience in the face of climate change, this review advocates for the adoption of an integrated pest management (IPM) approach combining innovative and traditional pest control strategies to enhance ecosystem services and fortify the resilience of agricultural systems. By interlinking these pivotal elements, this review presents a valuable perspective on the important pests affecting wheat and the currently used IPM practices, emphasizing the need for adaptive management in the context of evolving climate challenges. Full article