Search Results (2,682)

The utilization of saline–alkali lands and the competition between medicinal plants and grain crops are urgent issues. This study aimed to evaluate the effects of combined biochar and phosphogypsum application on soil physicochemical properties, microbial communities, and safflower growth, yield, and bioactive component accumulation in moderately saline–alkali soil of western Jilin, and to identify key soil factors driving these responses. To achieve this, outdoor pot experiments were conducted using safflower (Carthamus tinctorius L.), with the application of 1% biochar + 1% phosphogypsum to moderately saline–alkali soil. The results showed that the amendment significantly reduced bulk density (BD), pH, sodium adsorption ratio (SAR), total alkalinity (TA), and exchangeable sodium percentage (ESP), while increasing soil water content (SWC), soil organic matter (SOM), nitrogen, phosphorus, potassium, and beneficial ions. Soil sucrase, urease, alkaline phosphatase, and catalase activities were enhanced. Copiotrophic taxa (Pseudomonadota, Sphingomonas, Vicinamibacter) increased, whereas oligotrophic taxa (Gemmatimonadetes, Longimicrobium, Luteitalea) decreased, with stronger effects on bacteria than fungi. Safflower growth indices improved; leaf Na⁺/K⁺ ratio, superoxide radicals, and malondialdehyde decreased; and soluble protein, proline, and antioxidant enzyme activities increased. Bioactive components (hydroxysafflor yellow A, kaempferol) and yield reached 1.41%, 0.056%, and 343.23 mg/plant, representing 1.74–27.68-fold increases over moderate and mild saline–alkali soils. Correlation analysis identified SOM, total nitrogen (TN), available phosphorus (AP), BD, SWC, pH, SAR, TA, and ESP as key factors. In conclusion, co-application of 1% biochar and 1% phosphogypsum improves soil physicochemical and microbial properties, alleviates saline–alkali stress, and enhances safflower quality and yield. Full article

Climate change is expected to increase the frequency and severity of drought events in Europe, necessitating the identification of more water-efficient cropping systems. This study compared the evapotranspiration dynamics, water-use efficiency, and yield performance of maize (Zea mays L.) and grain sorghum (Sorghum bicolor L. Moench) under controlled field conditions using a Thornthwaite–Mather-type compensation evapotranspirometer. Three water regimes (100%, 50%, and 30% of optimal water supply) were applied during the reproductive stage, combined with weed-free and weed-infested treatments. Under moderate water deficit (50% water supply), grain sorghum maintained stable grain yield, while maize grain yield decreased by 17.98%. Under severe water deficit (30% water supply), grain yield reductions reached 36.04% in maize and 42.80% in sorghum. Grain sorghum consistently required less water and used 2.87–38.17% less water to produce 1 kg of grain compared to maize across treatments. Weed interference was associated with a lower yield and water-use efficiency in both species, while severe water deficit (70%) caused substantial declines in all measured parameters. Evapotranspiration was primarily driven by solar radiation and temperature, with reduced sensitivity under increasing water limitation. Overall, the results suggest that grain sorghum may represent a viable alternative to maize under moderate drought conditions; however, both crops require supplemental irrigation under severe water scarcity. The study highlights the importance of integrated weed management and provides novel insights into crop water-use dynamics under combined abiotic and biotic stress conditions. Full article

Background/Objectives: The high temperatures associated with climate change represent an important constraint for tomato production in tropical regions, affecting plant growth, reproductive development, and fruit metabolic composition. In this context, protected cultivation systems capable of modifying greenhouse microclimates may help reduce thermal stress and maintain crop productivity. Methods: This study evaluated the effects of two protective environments, diffuse agricultural film (AF) and twin-walled polycarbonate panels with laminar water flow (P), on the agronomic performance and fruit metabolic traits of five grape–tomato hybrids grown under tropical conditions. Microclimatic variables, vegetative growth, yield components, postharvest behavior, and fruit quality attributes were evaluated, with emphasis on carotenoid accumulation. Results: Compared with the agricultural film environment, the polycarbonate system reduced global radiation and photosynthetically active radiation (PAR) and was associated with an increase in yield of approximately 25%, an increase in fruit number of approximately 13%, and an 8% increase in fruit diameter. In addition, some hybrids cultivated under the polycarbonate system showed greater lycopene and β-carotene accumulation, indicating that microclimate moderation may favor carotenoid-related fruit quality depending on genotype. Principal component analysis revealed a clear separation between cultivation environments, with the polycarbonate system more closely associated with yield-related and canopy development traits, whereas the agricultural film environment was linked to biomass accumulation and selected physicochemical attributes. Among the evaluated hybrids, BS IGR0104, Jacy, and GI7545 showed the most favorable combination of agronomic performance and fruit quality traits. Conclusions: These results demonstrate the importance of climate-adaptive protected cultivation systems and hybrid selection for improving tomato productivity under tropical heat conditions. Full article

Malaysia plans to produce 80% of its rice requirement by 2030. To achieve the plan, new agronomic approaches have to be put in place to enhance the fertility of rice soils in the country. One of the options is to turn the infertile acid sulfate soils endemic in the low-lying coastal plains of Peninsular Malaysia into a new granary area. Using traditional agro-techs, rice yield in the area is below the national average of 4 t/ha/season. The low yield is due to soil acidity stress (pH < 4) together with Al³⁺ and/or Fe²⁺ toxicity. The critical pH for rice is 6, while the respective critical Al³⁺ and Fe²⁺ concentrations are 5.2 µM and 14.6 µM. The adverse conditions contributing to yield reduction can be resolved by applying appropriate soil amendments known to raise water pH, eliminating the toxic cations. The recommended agronomic practice is to apply ground magnesium limestone (GML) or ground basalt, or better still, apply GML or ground basalt in combination with bio-fertilizer, fortified with phosphate-solubilizing bacteria (PSB). The PSB increases water pH as well as helps rice plants secrete organic acids that reduce the toxic effects of Al³⁺ and Fe²⁺ via chelation. When pH rises >5, the toxic metals are precipitated, forming inert hydroxides. Ultimately, rice yield can be increased from 3 to 5 t/ha/season, which can last more than three consecutive cropping seasons. If this agro-tech is adopted throughout ASEAN, food security in the region will be sustained. Full article

Changes in environmental temperatures and water availability can disrupt plant–pollinator interactions by altering floral attractive and rewarding traits. Here, we investigated the effects of warming and drought on floral scent and rewards in Vaccinum corymbosum (an entomophilous crop), and how these changes affect pollinator behavior. Plants were exposed to two temperatures (24 °C and 28 °C) and two watering treatments (optimal watering, W+, and water stress, W−). We measured floral volatiles, pollen and nectar quantity, as well as the nutritional composition of pollen (C, carbon, and N, nitrogen percentage) and nectar (hexose-to-amino acids ratio). Bioassays with honeybees were conducted to assess responses to the attractive and rewarding traits specific to each treatment. Floral volatiles significantly increased at 28° W+; nevertheless, they declined under the combination of both warming and drought. Pollen and nectar production were only negatively affected by warming. Pollen’s nutritional composition was negatively affected by the interaction of both stresses, with greater reductions in % C and N occurring when both stresses were combined. We observed that the synthetic floral scent representing the blend emitted by flowers under 28° W+ conditions, at low concentrations, attracted the highest percentage of honeybees. Additionally, honeybees tended to visit artificial diets of pollen with a more nutritious composition (50% carbon and 6% nitrogen), as found in 24° W+. We showed that changes in the composition of floral scent and pollen in varieties of V. corymbosum affected pollinator preferences in laboratory bioassays. This study contributes to our understanding of how climate change may impact trophic interactions by showing that changes in floral traits are associated with alterations in pollinator preferences. Full article

Various strategies are being adopted to ensure sustainable fruit and vegetable production under the increasing population pressure and changing climatic conditions. Among these, grafting has emerged as an effective approach for improving crop performance without altering the genetic makeup of commercial cultivars. In this technique, a desirable scion is combined with a compatible rootstock possessing beneficial traits. When selected appropriately, such combinations can perform better than non-grafted plants, particularly under stress conditions. Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops worldwide due to its high economic and nutritional value. Grafting in tomato has been reported to enhance plant vigor, improve tolerance to abiotic and biotic stresses, and increase yield, often in the range of 15–30% under adverse conditions. However, the success of grafting largely depends on physiological compatibility between rootstock and scion. This review focuses on the physiological basis of rootstock–scion interactions in tomato, with an emphasis on water relations, nutrient uptake, and stress tolerance mechanisms. It also discusses current research gaps and highlights the need for a better understanding of the underlying physiological processes to improve the effectiveness of grafting in tomato production. Full article

Salinization of agricultural soils is a global problem causing crop yield declines. This impact is caused by osmotic and oxidative stress, which plants often rely on endophytic bacteria to overcome. A bacterial isolate from the roots of the halophyte Plantago salsa was studied between 2024 and 2026, and its ability to increase barley tolerance to moderate salt stress was determined. Based on 16S rRNA gene sequencing (1410 bp), the isolate PS-50.1 was identified as Providencia sp. It demonstrated key plant growth-promoting properties, including indole-3-acetic acid production (21.4 mg L⁻¹) and phosphate solubilization (69.0 mg L⁻¹). The strain supported barley growth at 7% NaCl. Inoculation of barley seeds with this strain (10⁸ CFU L⁻¹) significantly reduced moderate salt stress in plants both in vitro and in a pot experiment. Inoculated plants under salinity conditions had greater shoot length (+11.6%) compared to non-inoculated; higher pre-flag leaf fresh weight; demonstrated decreased levels of prooxidants (H₂O₂ by 44.8% and malondialdehyde by 31.8%), higher proline accumulation (up to 2.0-fold), and increased antioxidant enzyme activity (catalase by 26.6% and ascorbate peroxidase by 191%). Furthermore, inoculated plants showed 9.4% higher water use efficiency and photosynthetic rate (+5.5%) under salt stress compared to uninoculated plants. These results indicate that the halophytic strain Providencia sp. PS-50.1 is a promising candidate for the development of microbial preparations aimed at increasing crop productivity under saline conditions. Full article

Amid growing water scarcity, assessing agricultural water consumption and crop water footprint has become increasingly critical. This study aims to assess the water footprint of crops within the Shu–Talas River Basin, disaggregated into green and blue components. Using meteorological data from the 2000–2024 period, reference evapotranspiration (ET₀) and actual crop evapotranspiration (ET_c) were calculated according to the FAO methodology. The water footprint (WF_green, WF_blue, and WF_quant) was determined based on crop evapotranspiration, effective precipitation, and crop yields for maize, sugar beet, sunflower, and potato. It was found that total water consumption during the growing season ranges from 650 to 950 mm, with the blue water share exceeding 80%, reflecting the high dependence of agricultural systems on irrigation. The minimum WF_quant values were observed in sugar beet, while the maximum WF_quant values were recorded for sunflower. The study identifies crop yield, rather than absolute water consumption, as the key factor in water footprint formation. These findings and established patterns can be utilized to optimize cropping patterns and support sustainable agricultural water management in arid regions. Full article

Abiotic stresses limit crop productivity by disrupting water relations, carbon assimilation, nutrient acquisition, membrane stability, and redox homeostasis. Partial root-zone irrigation (PRI), commonly implemented as partial root-zone drying (PRD), is often viewed as a deficit-irrigation strategy to improve water-use efficiency; however, this view underestimates the biological consequences of spatial root-zone heterogeneity. This review evaluates PRI as a spatially structured, priming-like framework for crop adaptation to abiotic stress. Available evidence indicates that localized drying and wet-side water uptake can coordinate root sensing, hydraulic–chemical signaling, abscisic acid delivery, hormone crosstalk, xylem-mediated regulation, and stomatal control. Beyond gas exchange, PRI is associated with photosynthetic maintenance, osmotic adjustment, antioxidant and redox regulation, root architectural plasticity, nutrient acquisition, and metabolic reprogramming. Evidence is strongest for drought, whereas responses to low temperature, salinity, heat-associated evaporative demand, and combined stresses remain more context-dependent. Emerging work also links PRI to rhizosphere restructuring and microbiome shifts, but the causal mechanisms and field reproducibility remain unresolved. We argue that future progress requires matched PRI–deficit-irrigation comparisons, standardized switching thresholds, shared physiological and molecular readouts across crops, high-resolution root biology, and commercially realistic field validation. This framing distinguishes conserved physiological outcomes from mechanisms that may differ among crops, genotypes, and irrigation designs. Full article

Drought stress is the most pervasive abiotic constraint on global crop productivity, with projected intensification under climate change threatening the yields of staple crops including wheat, rice, maize, and legumes. Conventional breeding approaches have delivered limited gains against drought tolerance, constrained by the polygenic and multifactorial nature of stress adaptation, the complexity of genotype-by-environment interactions, and the inadequacy of field-based phenotyping under variable stress conditions. Omics technologies, including genomics, transcriptomics, proteomics, metabolomics, epigenomics, and phenomics, have substantially advanced the molecular dissection of drought tolerance by enabling high-resolution characterization of stress-responsive genes, regulatory networks, adaptive proteins, and metabolic reprogramming pathways. Specific traits targeted include root system architecture and depth, osmotic adjustment capacity through proline and glycine betaine accumulation, antioxidant defense mechanisms, ABA-mediated stomatal regulation, LEA protein accumulation, epigenetic stress memory, and yield stability under water deficit. This review systematically examines omics-based strategies for drought stress mitigation across major crops, highlighting individual omics contributions, multi-omics integration frameworks, computational tools including machine learning and AI-driven predictive modelling, and translational breeding applications. Case studies in wheat, rice, maize, and legumes illustrate how omics-driven approaches accelerate precision breeding for drought resilience through marker-assisted selection, genomic selection, and CRISPR-based gene editing. Challenges including data integration complexity, high implementation costs, limited cross-species transferability, and the need for field-scale validation of microbiome-based strategies are critically addressed. Future perspectives encompassing single-cell and spatial omics, AI-driven predictive breeding, digital agriculture integration, and international data governance frameworks are discussed. By aligning with climate-smart agriculture principles, multi-omics approaches provide a robust and transformative foundation for developing drought-resilient crop cultivars suitable for water-limited production systems worldwide. Full article

Tomato, cucumber, and sweet pepper represent the backbone of greenhouse vegetable cultivation. Over recent decades, developments in agronomic practices have been central to improving yield, resource-use efficiency, resilience to abiotic stresses, and product quality. This review synthesizes dispersed evidence on water and nutrient management, cultivar improvement, grafting, canopy management, biological inputs, and postharvest-oriented agronomy, while highlighting that the three crops exhibit markedly different responses to these practices. These responses are primarily driven by crop-specific differences in source–sink balance, root-zone regulation, canopy architecture, reproductive stability, and postharvest metabolic regulation. Tomato typically demonstrates substantial improvements in yield and water use efficiency under optimized fertigation strategies, with canopy management additionally promoting source–sink balance and stress resilience. Cucumber, by contrast, is particularly sensitive to water deficits, salinity, and nutrient imbalances, necessitating stricter control of irrigation and fertilization to maintain stable root-zone water flux and transpiration dynamics. Sweet pepper often exhibits greater physiological complexity, as yield stability is strongly influenced by microclimate-sensitive metabolic and ionic balance, frequently associated with trade-offs in quality, including firmness, color development, and nutritional composition. The success of grafting, microbial inoculants, and biostimulants further varies considerably among crops, reinforcing the need for crop-specific strategies rather than generalized approaches. Postharvest-oriented agronomy, involving the regulation of nutrient supply, harvest timing, and canopy structure, is becoming increasingly important for prolonging shelf life and improving quality in line with market demands. Sustainability-oriented practices, including nutrient recycling and water-saving strategies, additionally contribute to reducing environmental burdens while maintaining profitability. By identifying species-specific physiological constraints and agronomic priorities, this review highlights that crop-customized and physiologically integrated management strategies are essential for improving productivity, resilience, and quality in protected cultivation. Full article

Accurate assessment of soil moisture is essential for enhancing irrigation efficiency and promoting sustainable agriculture. This study was conducted at Punjab Agricultural University, Ludhiana (PAU), to investigate the spatial and depth-wise variability of soil moisture across 30 field sites by using field measurements, geospatial-based (inverse distance weighting: IDW) interpolation, and machine learning techniques. Soil moisture was recorded at four depth intervals, including 0–15 cm, 15–30 cm, 30–45 cm, and 45–60 cm. The surface layer (0–15 cm) exhibited the highest variability due to evaporation and irrigation timing, with values ranging from 4.5% to 16.0%. Deeper layers showed more stable moisture retention, particularly at sites with intensive irrigation and crop cover, such as L11 (wheat), L22 (Gobhi Sarson), and L25 (wheat), where the moisture levels exceeded 14% at 45–60 cm depth, supporting suitability for deep-rooted crops. Supervised machine learning models, namely decision tree (DT), random forest (RF), and logistic regression (LR), were employed to classify soil moisture into low, medium, and high categories. The highest classification accuracy (88.9%) was achieved by the decision tree at 30–45 cm and logistic regression at 15–30 cm. Shallow layers exhibited frequent misclassification between medium and high classes, indicating surface-induced variability. Unsupervised clustering using K-means (k = 4) and hierarchical methods effectively delineated distinct soil moisture zones aligned with land use, irrigation history, and crop cover. The combination of geospatial analysis, depth-specific field data, and machine learning models provides an integrated framework for precision soil moisture assessment. This approach supports site-specific irrigation scheduling and water resource optimization, which are particularly critical for groundwater-stressed regions like Punjab. The novelty of this study lies in integrating depth-specific field-based soil moisture observations with geospatial interpolation and machine learning-based classification and clustering approaches to improve subsurface moisture characterization for precision irrigation management. Full article

Drought stress is one of the most severe constraints affecting wheat production worldwide. Under these conditions, the development of sustainable and economically viable strategies, such as seed priming, is essential to improve wheat performance and drought resilience. The present study carried out a greenhouse experiment on four Mediterranean durum wheat cultivars (Triticum turgidum ssp. durum Desf), i.e., Karim (Kr) and Khiar (Kh) from Tunisia and Espelta (Esp) and Mocho (Mo) from Spain, subjected to drought stress conditions, and using primed abscisic acid (ABA), indole-3-acetic acid (IAA), melatonin (Mlt), and salicylic acid (SA), and non-primed seeds. In order to assess the physio-biochemical responses of durum wheat, such as plant growth, chlorophyll, relative water content (RWC), water potential (Ψw), osmotic potential (Ψs), proline, soluble sugars, starch, glycine betaine, hydrogen peroxide, malondialdehyde, and antioxidant enzyme activities. The results showed that water stress significantly reduced plant growth, SPAD index, RWC, Ψw, and Ψs, while upregulating H₂O₂ and MDA levels, depending on the wheat cultivars. Soluble sugars decreased, whereas starch, glycine betaine, and proline accumulated in all cultivars. Superoxide dismutase activity was reduced (24–37%) under water stress as compared to the control condition, while APX, CAT, and POD activities significantly increased. Among the cultivars, Esp exhibited the greatest plasticity in response to water deficit, whereas Kh appeared to be most sensitive. Furthermore, the present results revealed that the priming durum wheat seeds with ABA, IAA, Mlt, and SA improved leaf hydration, particularly through soluble sugar accumulation. Seed priming also alleviated oxidative stress by reducing H₂O₂ and MDA levels and stimulating APX, CAT, POD, and SOD activities. Plants grown from non-primed seeds of Spanish and Tunisian cultivars exhibited differential responses to drought stress, and those derived from primed seeds showed varying degrees of enhanced drought tolerance. Espelta demonstrated a high potential for stress tolerance and responsiveness to priming, followed by Karim, whereas Khiar was the most sensitive cultivar. Overall, the cultivars can be ranked in decreasing order of stress tolerance as Esp > Kr > Mo > Kh. These findings highlight the potential of phytohormone-based seed priming as an efficient and practical approach to enhance drought resilience in durum wheat, offering promising prospects for improving crop performance and stability under increasingly water-limited conditions in the era of climate change. Full article

Salt stress remains a major global stress factor among abiotic stresses limiting crop production. Salt stress is a major nutritional challenge, with poor agricultural production characterized by high soil sodium (Na⁺) levels in soil and plants. Soil salinity negatively affects plants through both osmotic effects and ionic toxicity. Hence, one of the main aims of agricultural scientists is to develop eco-friendly, sustainable solutions to alleviate soil salinity. Over the past decades, several studies have recommended biochar as a vital sustainable soil amendment to alleviate the negative consequences of soil salinity. Thus, this review builds on the literature on biochar-mediated alleviation of salt stress. Biochar is a carbon-rich material produced from biomass and feedstock via pyrolysis under little or no oxygen conditions. Due to its unique characteristics, such as high carbon, high surface area with porous and aromatic structure, high pH, high stability, cation exchange capacity, and water and nutrient retention capacity, it is considered an alternative for salt stress alleviation. Moreover, biochar facilitates sodium ion (Na⁺) adsorption, reduces Na⁺ uptake, and increases potassium ion (K⁺) uptake, enhancing nutrient cycling, helping plants maintain ionic balance and osmotic regulation. This, in turn, significantly increased the activity and diversity of soil microorganisms, enhanced their adhesion, and promoted their growth, thereby strengthening the plant’s salt resistance. Moreover, biochar-mediated improvements in microbial community dynamics and changes in the physical and biological properties of soil contribute to overall plant and soil health under salt stress. Hence, the present review aims to decipher the holistic patterns of biochar on soil and plant health, changes in physiological and defense mechanisms, plant hormones and signaling mechanisms, and the status of modified biochar under salt stress. Thus, the present review will pave the way for the production of salt-resilient crops with enhanced salinity tolerance. In conclusion, the use of biochar-based fertilizers and modified biochar enhanced microbial community dynamics in soil health homeostasis and soil fertility for agricultural production and food security. Full article

Freshwater lakes in Mediterranean regions are highly sensitive to climatic variability, particularly to droughts intensified by rising temperatures and increasing atmospheric evaporative demand. This study investigates drought variability and ecosystem responses in the Trichonida basin, the largest natural freshwater system in Greece, using an integrated approach that combines the Standardized Precipitation Evapotranspiration Index (SPEI) at multiple time scales with satellite-derived Normalized Difference Vegetation Index (NDVI), Crop Water Stress Index (CWSI), and lake surface water temperature. SPEI analysis revealed increasingly recurrent and persistent drought conditions in recent years, especially at medium- and long-term scales. NDVI exhibited pronounced seasonal variability and a moderate long-term increase at the basin scale, largely associated with agricultural activity and irrigation practices, while sharp declines were observed during severe drought episodes. CWSI showed strong seasonal patterns characterized by recurrent summer water stress events, but no significant long-term trend. Correlation analysis indicated positive relationships between NDVI and SPEI at medium- to long-term time scales, and significant negative correlations between CWSI and SPEI at short and medium time scales. A strong relationship between NDVI and CWSI further suggests the sensitivity of vegetation greenness to water stress, particularly during summer and autumn. Lake surface water temperature exhibited seasonal warming trends that coincided with periods of increased vegetation water stress. Drought-related water risks arise for calcareous fens dominated by Cladium mariscus in the Lake Trichonida system, a habitat of high conservation value, whose productivity is strongly seasonally controlled and closely linked to thermal dynamics. Overall, the combined multi-indicator analysis provides valuable insights into drought impacts and seasonal ecosystem vulnerability in Mediterranean lake basin environments, highlighting the importance of integrated monitoring frameworks for sustainable freshwater ecosystem management under increasing climatic variability. Full article