Search Results (6,360)

Previously, we reported that mammalian cells, specifically human dermal fibroblasts (HDFs), could be transdifferentiated by lactic acid bacteria (LAB). Later, we observed that HDFs incorporated LAB-derived ribosomes, forming the ribosome-induced cell clusters (RICs) and transdifferentiating into cells derived from all three germ layers. Based on this insight, we hypothesized that incorporating ribosomes into non-mammalian cells could reveal the universality of this mechanism and open the door to commercial applications. Our current study demonstrates that ribosome incorporation can transdifferentiate chick primary muscle-derived cells (CMCs) into adipocytes, osteoblasts, and chondrocytes. Furthermore, the culture medium supernatant from ribosome-incorporated CMCs was found to significantly enhance CMC’s proliferation. RNA-seq analysis revealed that RICs-CMC exhibit increased expression of genes related to multi-lineage cell growth. In addition, we developed a novel technological shift in meat production—the “CulNet System”—which replicates organ interactions within mechanical systems for cell-cultured meat production. While significant efforts are still required to implement this technology in a cost-effective manner, we believe that combining the “CulNet System” with ribosome-incorporated multipotent cells that have prolonged culture capability could substantially improve the scalability and cost-effectiveness of cultured chicken meat production. This report highlights a promising approach for cell-culture-based meat production, offering a sustainable alternative to traditional methods. Full article

Background/Objective: The priority task of each country is to ensure the protection of and improvement in its people’s health. One of the key aspects of health is related to food culture, consuming foods that ensure growth and normal development in a person and also prevent diseases. The consumption of foods that contain essential nutrients and functional foods, which include those containing probiotics, is the basis of a healthy diet. Methods: A structured anonymous interview was conducted with 90 parents from the Roma minority group to assess the nutritional culture of children from these families. The knowledge of Roma parents about probiotics, as well as their attitudes to apply them to their children, was examined. The survey was based on a questionnaire that was distributed among the respondents, Roma students, under the guidance of a mentor. Roma students study health specialties and are also participants in a Scholarship Programme. Results: We have established that the daily diet of children from this minority group consists of foods containing hydrogenated fats and sugar, which was indicated by 53.3% of respondents, and only 28.9% of respondents noted that their children consume fresh fruit every day. We have established that less than half of the children (35.6%) consume yoghurt daily as a source of valuable probiotics. We applied a nonparametric Kruskal–Wallis test and found statistically significant differences in the respondents’ knowledge of probiotics (χ² = 16.186, p = 0.001): those receiving secondary specialized education were better informed, but education has not affected their knowledge of the health benefits of probiotics (χ² = 5.462, p = 0.141). Children from minority groups tend to eat unhealthy foods. Conclusions: The role of Roma students studying health specialties as participants in the Scholarship Programme is to assist parents in forming a nutritional culture in their children. Roma students, due to their ethnic and cultural proximity and potential to be health professionals, contribute to sustainable health improvements among the Roma community as a whole. Full article

Background: Gender-based violence has serious health impacts on survivors and is perpetuated by cultural norms, patriarchy, and traditional values. This study explored women’s (survivors) views, attitudes and the impact of gender-based violence on their health in a selected rural village within the Collins Chabane Municipality, Vhembe District, Limpopo Province. Methods: A qualitative descriptive design was used, involving 20 women aged 18 to 59. Semi-structured in-depth interviews were conducted, and data were analysed thematically using ATLAS.ti 8, based on Braun and Clarke’s six-step framework. Ethical considerations were prioritised due to the sensitive nature of the research. Results: The results revealed that women in the Collins Chabane Municipality experience multiple forms of GBV, including physical, sexual, emotional, and financial abuse, which severely impacts their mental and physical health. Additionally, socioeconomic factors like unemployment and financial dependence exacerbate their vulnerability, making it difficult to escape abusive relationships. Conclusions: Cultural norms, patriarchal beliefs, and limited community support intersect to shape women’s experiences and responses to abuse. Full article

One of the best restorative treatment options for large carious lesions is the placement of stainless-steel crowns (SSC), but there are few studies evaluating if there is any change in the microbiota in teeth restored with SSCs. In order to asses if any difference exists, 33 children between 4 and 10 years were studied. One primary molar restored with an SSC as well as one healthy primary molar were selected from each child. Subgingival plaque was collected with a curette and cultured on horse blood agar (Columbia) and selective and nonselective media. A quantitative analysis was performed by means of the counting of the colony-forming units per milliliter (cfu/mL) grown in the nonselective media and compared with the bacterial load measured by means of a 16S qPCR with bacterial universal primers. A descriptive statistical analysis was performed to evaluate the results. No significant differences were observed in the total 16S qPCR according to sample type. Streptococci were observed in all the studied children. Porphyromonas gingivalis was observed in 18% of patients and Prevotella intermedia in 42%. Campylobacter was observed in 81% and Neisseria in 88%. C. albicans was observed in only one patient. No significant differences were found between both groups. Part of the child population studied had anaerobic bacteria. There is no clear association between the presence of periodontopathogens and SSC. Full article

This study developed electrosprayed deferoxamine (DFO)-loaded poly(lactic-co-glycolic acid) microspheres (DFO-MS) combined with a sucrose acetate isobutyrate (SAIB) depot (DFO-MS@SAIB) for bone-defect repair, targeting the coordinated regulation of angiogenesis and osteogenesis in vascularized bone regeneration—where new blood vessels support functional bone integration. In vitro/in vivo evaluations confirmed its dual pro-angiogenic and pro-osteogenic effects via HIF-1α pathway activation. Background/Objectives: Emerging evidence underscores the indispensability of vascularization in bone-defect repair, a clinical challenge exacerbated by limited intrinsic healing capacity. While autologous grafts and growth-factor-based strategies remain mainstream, their utility is constrained by donor-site morbidity, transient bioactivity, and poor spatiotemporal control over angiogenic–osteogenic coupling. Here, we leveraged DFO, a hypoxia-mimetic HIF-1α stabilizer with angiogenic potential, to engineer an injectable DFO-MS@SAIB depot. This system was designed to achieve sustained DFO release, thereby synchronizing vascular network formation with mineralized tissue regeneration in critical-sized defects. Methods: DFO-MS were fabricated via electrospraying and combined with SAIB (DFO-MS@S) to form an injectable sustained-release depot. Their physicochemical properties, including morphology, encapsulation efficiency, degradation, release kinetics, and rheology, were systematically characterized. In vitro, the angiogenic capacity of HUVECs co-cultured with DFO-MS was evaluated; conditioned HUVECs were then co-cultured with BMSCs to assess the BMSCs’ cytocompatibility and osteogenic differentiation. In vivo bone regeneration in a rat calvarial defect model was evaluated using micro-CT, histology, and immunohistochemistry. Results: The DFO-MS@SAIB system achieved sustained DFO release, stimulating HUVEC proliferation, migration, and tubulogenesis. In a Transwell co-culture model, pretreated HUVECs promoted BMSC migration and osteogenic differentiation via paracrine signaling involving endothelial-secreted factors (e.g., VEGF). HIF-1α pathway activation upregulated osteogenic markers (ALP, Col1a1, OCN), while in vivo experiments demonstrated enhanced vascularized bone regeneration, with significantly increased bone volume/total volume (BV/TV) and new bone area compared with controls. Conclusion: The DFO-MS@SAIB system promotes bone regeneration via sustained deferoxamine release and HIF-1α-mediated signaling. Its angiogenesis–osteogenesis coupling effect facilitates vascularized bone regeneration, thereby offering a translatable strategy for critical-sized bone-defect repair. Full article

Migration is a multidimensional process that reshapes identities and communities. This article adopts a polyhedral framework inspired by Pope Francis’s Laudato si’ and Michel Foucault’s concepts of “subjectivation” and the “polyhedron of intelligibility”. Both emphasize spirituality as a transformative force in individuals’ lives and a concept that connects philosophy and theology to support resilience among migrant populations. Using Portugal as a case study, the research examines migration’s historical and contextual landscape and its discursive framework. Through a Lisbon-based research project of interviews with migrants, the study explores the concept of spiritual integration by presenting how spirituality functions to preserve cultural identity while facilitating integration without full assimilation into the host community. Spirituality includes many rules and choices regarding ways of life; therefore, the interview projects’ migrants interpret the concept of spiritual integration in a subjective and polyhedron manner. Creating strong ties to their homes, traditions, cultures, spirituality, sports, and culinary practices, as well as practicing, sharing, and teaching these practices, protects them from total subjection, while learning the host society’s customs and rituals helps them to fit in. The findings show that spirituality serves as an integrational tool, a coping mechanism, and a form of resistance, providing a space for migrants to address and overcome challenges. The article emphasizes the importance of integration policies to create a “safe place” of inclusivity within host communities. Full article

Background:Intervertebral disc degeneration (IVDD) is a major cause of chronic back pain. Recent studies suggest that ferroptosis, a form of cell death, contributes to the degeneration of nucleus pulposus cells (NPCs). This study explores a novel therapeutic strategy using cell-free fat extract (CEFFE), rich in cytokines, to mitigate IVDD by inhibiting oxidative stress-induced ferroptosis. Methods: In vitro, NPC degeneration was induced by TNF-α/TBHP. The effects of CEFFE on matrix metabolism were evaluated using Western blotting, RT-qPCR, and high-density culture, with regenerative effects measured via CCK-8 assays. Ferroptosis was assessed by Western blotting, immunofluorescence, and electron microscopy. In vivo, rats with caudal IVDD were treated with CEFFE for 4 weeks, and therapeutic efficacy was evaluated through imaging and histological analysis. Results: In vitro, CEFFE reduced TNF-α-induced inflammation and promoted matrix synthesis by inhibiting MAPK and NF-κB pathways. It also activated NRF2 to prevent TBHP-induced ferroptosis. In rats, CEFFE facilitated nucleus pulposus repair and significantly slowed disc degeneration. Conclusions: CEFFE is a promising strategy to delay IVDD progression by inhibiting ferroptosis, offering potential therapeutic benefits for disc degeneration. Full article

V. S. Naipaul’s 1975 novel Guerrillas is the earliest example of Caribbean fiction that purports to provide a realistic depiction of Trinidad’s brief but historically significant Black Power movement. Naipaul was an Indo-Trinidadian expatriate who immigrated to the U.K. in 1950 and remained there until his death in 2018. He was famously Anglophilic; and given his notorious insistence that culturally the West Indies are derivative, not creative, it is unsurprising that Naipaul depicts Black Power as an empty form that Trinidad and Great Britain import to their detriment from the U.S. In its fictionalization of the story of a real-life figure on the periphery of Black Power, Guerrillas presents Black Power’s presence in Trinidad and the UK as a failure and a sham. My article traces Naipaul’s transformation of what was originally a journalistic account into his novel Guerrillas in order to highlight the tendentiousness of his representation of Trinidadian Black Power. The plot of the novel repurposes the crux of Naipaul’s essay “The Killings in Trinidad” in which he reports how a Trinidadian Black Power poseur known as “Michael X” conspired in the January 1972 murder of a white woman named Gale Ann Benson. Crucial to Naipaul’s dismissal of Black Power as a derivative fiction, this article argues, is the fraudulent Michael X, himself a mimic man par excellence in his embodiment of Black Power as an empty and parodic form devoid of original content. I demonstrate how Naipaul’s marginalization of Caribbean Black Power depends on formal mimicry and on his selection of this marginal player/mimic man as representative of the movement in Trinidad. Full article

Historic monument buildings represent, in a three-dimensional form, the expression of civilization, culture, and progress across various historical periods. Restauration works on such structure must achieve a performance level that preserves the value expression of parameters associated with criteria derived from both the historical substance and the requirements for strength and stability. National and international regulations (such as UNESCO conventions) play an essential role in heritage consolidation. These frameworks set standards for conservation practices and promote the importance of protecting historic heritage, which is an expression of cultural identity and community history. The research findings presented in the paper refer to a case study, Costache Conachi Manor, a building classified as a historic monument, located in Galați County, Romania. The investigations concern the processes and methods used to identify the current condition of the building, which includes the structural aspect and the materials used, in order to ensure compatible restoration works while respecting and enhancing originality. Overall, these advanced research techniques not only help to accurately characterize the materials used in the historic Costache Conachi Manor masonry structure but also play a vital role in developing knowledge of restoration and conservation practices. Full article

Kimchi is a fermented Korean food typically made with napa cabbage, garlic, radish, ginger, and chili pepper. It is becoming increasingly popular due to its flavor, high fiber content, and purported probiotic benefits. The microbial ecology of the fermentation community has been extensively studied, though what’s less understood is how its microbial community changes when nutrients or pathogens are introduced. To study this, we used gnotobiotic cabbage media inoculated with a kimchi starter culture as a model system. These inoculated samples were exposed to E. coli or Bacillus cereus, with or without added nutrients in the form of tryptic soy broth (TSB). We tracked pH, colony-forming units (CFUs), and community composition over time. We also used Oxford Nanopore sequencing to analyze the 16S rRNA gene (V4–V9), followed by use of the Emu algorithm for taxonomic assignments. As expected, LABs suppressed pathogens, but this effect was weaker early on in the nutrient-rich condition. Pathogen exposure changed the overall community—Lactobacillus species became more common, and Leuconostoc mesenteroides less so. Interestingly, adding nutrients alone caused similar microbial shifts to those seen with pathogen exposure. This could suggest that nutrient levels have a larger impact on the final microbiome structure than direct microbial competition. Together, these findings suggest that monitoring total microbial composition, and not just the presence of pathogens, may be important for ensuring kimchi fermentation reproducibility. Full article

Background: During the COVID-19 pandemic, the emergence of multidrug-resistant (MDR) pathogens, driven by heightened antibiotic usage and device-associated infections, has posed significant challenges to healthcare. This study reports an outbreak of Proteus mirabilis producing NDM-5 and CTX-M-15 β-lactamases in a hospital in Buenos Aires, Argentina, from October 2020 to April 2021. To our knowledge, this represents the first documented outbreak of NDM-5-producing P. mirabilis in the country. Methods: A total of 82 isolates were recovered from 40 patients, with 41.5% from blood cultures and 18.3% from respiratory and urinary samples, among others. Antimicrobial susceptibility testing, PCR-based methods, and MALDI-TOF MS cluster analysis were conducted. Whole genome sequencing (WGS) was performed to characterize the MLST, resistome and plasmid content. Biofilm formation assays and in vitro rifampicin susceptibility tests were also conducted. Result: Most isolates exhibited resistance to carbapenems, cephalosporins, aminoglycosides, and fluoroquinolones, while retaining susceptibility to aztreonam. Genetic analysis confirmed the co-presence of the bla_NDM-5 and bla_CTX-M-15 genes. Clonal relationships was supported by PCR-based typing and MALDI-TOF MS cluster analysis. WGS revealed a resistome comprising 25 resistance genes, including rmtB and both β-lactamases, as well as the presence of an incomplete IncQ1 replicon associated with multiple resistance determinants. MLST classified this clone as belonging to ST135. Despite the biofilm-forming capacity observed across strains, rifampicin demonstrated potential for disrupting established biofilms at concentrations ≥32 µg/mL in vitro. The MDR profile of the outbreak strain significantly limited therapeutic options. Conclusions: This study highlights the growing threat of NDM-producing P. mirabilis in Argentina. The absence of surveillance cultures from the index case limits insights into the outbreak’s origin. These findings underscore the importance of integrating genomic surveillance into infection control protocols to mitigate the spread of MDR pathogens. Full article

This article examines Marcel Janco’s Holocaust drawings, positioning them within the broader discourse of Holocaust representation, trauma, and avant-garde aesthetics. Created in response to the Bucharest Pogrom of January 1941, these works resist both forensic realism and pure abstraction, instead embodying rupture, instability, and fragmentation. Janco’s grotesque distortions neither document events with the precision of testimony nor dissolve into conceptual erasure; rather, they enact the instability of Holocaust memory itself. This essay argues that Janco’s Holocaust works, long overshadowed by his modernist and Dadaist contributions, challenge dominant frameworks of remembrance. Through comparative analysis with artists, such as David Olère, Anselm Kiefer, and George Grosz, it situates Janco’s approach at the limits of witnessing, exploring how his figures embody violence rather than merely depict it. While Olère reconstructs genocide through forensic detail and Kiefer engages with the material traces of memory, Janco’s grotesque forms share an affinity with Grosz’s politically charged distortions—though here, fragmentation serves not as critique but as testimony. Furthermore, the study interrogates the institutional and critical neglect of these works, particularly within Israeli art history, where they clashed with the forward-looking ethos of abstraction. By foregrounding Janco’s Holocaust drawings as both aesthetic interventions and acts of historical witnessing, this article repositions them as crucial yet overlooked contributions to Holocaust visual culture—demanding recognition for their capacity to unsettle, resist closure, and insist on the incompleteness of memory. Full article

Theaters in ancient Pompeii played a vital role in Roman entertainment, shaping the auditory experiences of spectators. This study examines the acoustic properties of the Amphitheater, the Grand Theater, and the Odeon using impulse response (IR) analysis to reconstruct their soundscapes. Next, the study considers the impact of typical musical instruments, vocal performances, and ambient sounds—such as gladiatorial combat—on these spaces’ acoustics. Findings reveal significant differences in reverberation times, sound clarity, and spatial characteristics, shaped by each theater’s design. These sites, preserved after the eruption of Mount Vesuvius, provide a unique opportunity to analyze how architecture influenced sound propagation. The Amphitheater, with its vast open structure, enhanced large-scale events, while the Grand Theater’s semi-enclosed design balanced musical and theatrical performances. The Odeon, the most intimate of the three, prioritized speech clarity. These insights highlight how Roman architects engineered performance spaces to accommodate diverse entertainment forms. By combining acoustic measurements with historical context, this research offers a deeper understanding of ancient Pompeii’s soundscapes and the auditory experiences of its inhabitants and offers insights for composition and soundscape creations that take inspiration from these prominent historical architectural and cultural icons whilst exploring its potential within contemporary immersive listening practices. Full article

Public cultural facilities are the cornerstone of the construction of the public cultural service system. Exploring the spatial pattern of public cultural service facilities is significant for clarifying regional differences in public cultural services, optimizing the allocation of urban cultural facilities, and promoting the equalization of public cultural services. This study constructs a dual-dimensional equalization evaluation system of geographical density and per capita quantity to reveal the spatial mismatch phenomenon of public cultural facilities in Xinjiang. Using methods such as the nearest neighbor index and kernel density analysis, combined with the geodetector, the distribution patterns of public cultural facilities in 14 prefectures and cities in Xinjiang are systematically analyzed. The results show that public cultural facilities in Xinjiang exhibit significant agglomeration characteristics, with museums having the most prominent spatial agglomeration degree (NNI = 0.523) and imbalance degree (S = 0.284). A spatial pattern centered on Urumqi characterized by “dense in the northwest and sparse in the southeast” has formed. There exists a spatial mismatch phenomenon between high-density and low-per capita population and low-density and high-per capita population in terms of geographical density and population distribution. Population size is the key factor in facility distribution, while cultural demand and economic level are the main factors, and fiscal capacity and education level are secondary factors, with transportation conditions being general factors. In this paper, we analyze the spatial differentiation characteristics of public cultural facilities in Xinjiang and the influencing factors in order to provide typical cases and practical references for optimizing the allocation of urban cultural facilities and promoting their equalization. Full article

Background: Chronic rhinosinusitis (CRS) affects 5.5–28% of the population and is primarily an inflammatory disease, with microbiota potentially playing a key role. Understanding microbial pathogens and resistance patterns is crucial for effective management. This study aimed to evaluate the incidence of Gram-negative rods in CRS in adults as a part of a prospective microbiological study. Methods: Over one year, paranasal sinus mucosa samples from CRS patients and nasal concha samples from controls were analyzed. Cultivable bacterial flora was assessed using culture-based methods. Biofilm formation was evaluated via a microtiter-plate assay, and antibiotic susceptibility was tested using the disk diffusion method. Results: Tissue samples from 74 CRS patients and 47 controls yielded 198 bacterial strains. Gram-positive cocci dominated, while Gram-negative rods accounted for 17.6%, with Escherichia coli, Klebsiella oxytoca, and Citrobacter spp. being most common. All Gram-negative rods formed biofilms in vitro. They were susceptible to cefotaxime, aztreonam, ciprofloxacin, and meropenem but showed varying sensitivity to ampicillin (20–67%), tigecycline (40–57%), and amoxicillin/clavulanic acid (73–83%). Conclusions: The result of this study underlines that treatment of CRS should be based on the result of drug susceptibility testing of the isolated microorganism. Full article