Search Results (7,509)

In historic districts, the audiovisual environment plays an important role in shaping both cultural expression and spatial experience. However, the influence of acoustic and visual environmental factors on perceived street vitality remains insufficiently understood. Taking the Shangxiahang Historic District in Fuzhou as a case study, this paper employs on-site sound pressure level measurements, panoramic visual data collection, questionnaire surveys, principal component analysis, correlation analysis, and multiple regression analysis to systematically examine the effects of acoustic and visual environmental factors on perceived street vitality. The results indicate that traditional cultural sounds and natural sounds have a significant positive impact on perceived street vitality, while construction noise and tour guide’s horn sound exhibit negative effects. Regarding the visual environment, street and alley spaces, traditional architecture, greenery, and the sky are all important factors in promoting perceived street vitality. Further regression analysis reveals that the perception rate of street and alley spaces has the strongest influence, followed by the perception rate of traditional architecture, the perceived frequency of folk activity sounds, preference for greenery, and the perception rate of the sky. These findings demonstrate that perceived street vitality in historic districts does not depend on a single environmental factor but rather arises from synergistic interaction between culturally meaningful acoustic cues and legible spatial forms. These results offer practical implications for multisensory design and vitality-oriented regeneration in historic districts. Full article

Background/Objectives: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive and lethal forms of malignant neoplasm of the endocrine system, and osteopontin (OPN) has been shown to be aberrantly expressed in this tumor type. Among the five OPN splicing isoforms (OPN-SI), OPN-4 has been recently reported in several tumor types, including ATC, but its functional role(s) have not yet been elucidated. Methods: To characterize OPN-4 roles in ATC cells, OPN-4 was ectopically overexpressed in the c643 ATC cell line, generating the c643/OPN-4 cells. OPN-roles were evaluated by cell functional assays, including cell proliferation and viability, using Carboxyfluorescein Succinimidyl Ester (CFSE), crystal violet, and trypan blue assays. For migration, clonogenicity, cell cycle and apoptosis assays were used. For assessment, c643/OPN-4 cells were cultured in two-dimensional (2D) monolayers or three-dimensional (3D) spheroids with the latter being maintained in a bespoke microfluidic system. Results: OPN-4 overexpression led to a significant reduction in cell proliferation, viability, migration and clonogenicity. c643/OPN-4 cells displayed a significant accumulation in the G0/G1 phase and a decrease in the S phase of the cell cycle; however this did not affect cell death or the expression levels of other OPN-SI. In a spheroid model of c643/OPN-4 cells, no significant differences were found in spheroid size or viability when compared to those formed by control cells. Notably, OPN-4 overexpression enhanced the effects of sorafenib on cell viability under dynamic treatment conditions involving continuous perfusion. Conclusions: These early findings point to the fact that OPN-4 may reduce some aspects of tumor progression features in ATC cells and open new avenues for investigating OPN-4 as a biomarker of therapeutic response in personalized treatment strategies. Full article

Petroleum contamination of soils remains a significant environmental problem in boreal regions, where low temperatures constrain natural attenuation processes and complicate bioremediation. Nitrogen biostimulation is widely used to enhance petroleum hydrocarbon degradation; however, the combined effects of temperature regime, nitrogen form, contamination level, and nitrogen dosage remain insufficiently resolved for sandy podzolic soils of northern regions. This study investigated nitrogen-assisted biostimulation of petroleum-contaminated sandy podzolic soil collected in the Khanty–Mansi Autonomous Okrug (Western Siberia, Russia) using a factorial experimental design. Soil samples were artificially contaminated with crude oil at concentrations of 25, 50, and 100 g kg⁻¹ and incubated under warm and cold temperature regimes. Two nitrogen sources, urea and ammonium nitrate, were applied at several dosages. Changes in residual petroleum hydrocarbon content were monitored together with the abundance of culturable microorganisms under the applied cultivation conditions at the intermediate contamination level on day 60. Nitrogen supplementation enhanced petroleum hydrocarbon removal relative to the untreated control, but the magnitude of the effect depended substantially on temperature, nitrogen form, and contamination level. Under the tested conditions, ammonium nitrate was generally associated with stronger hydrocarbon removal than urea, particularly at the intermediate contamination level (50 g kg⁻¹). The results indicate that the response to nitrogen biostimulation in sandy boreal soils is controlled by interacting experimental factors rather than by nitrogen addition alone. These findings improve the positioning of nutrient-assisted remediation in cold-region soils and provide a basis for future mechanistic and field-scale studies. Full article

Drawing an analogy to the 5th century heresy of Nestorianism, this paper argues that the separation of life from faith in the modern era constitutes a kind of functional Nestorianism, that negates the universality of the Christian claim and renders such a claim. The paper argues that, in their form and function, many Catholic educational institutions unwittingly adopt this functional Nestorianism by means of a structural compartmentalisation of the faith into confined spaces within the curriculum and within the institutional imagination and that in doing so, such institutions risk mirroring—at the level of educational practice—and thus perpetuating, what Pope Paul VI referred to as ‘the drama of our time’, that being, ‘the split between the Gospel and culture’. The paper concludes by offering six Christocentric principles to guide curriculum development and implementation in Catholic educational institutions. Full article

Background: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system and is the leading cause of neurological disability. Currently, the main strategy for MS therapy is the use of disease-modifying therapies (DMTs). If low-efficacy DMTs are ineffective, patients are transferred to high-efficacy DMTs, which possess more severe side effects associated with immunosuppression. Therefore, the search for new add-on therapies for MS that can enhance the effect of low-efficacy DMTs is relevant. Curcumin, being a natural polyphenol, has immunoregulatory properties and a favorable safety profile. In addition, micellar forms of curcumin can increase its bioavailability. We studied the effect of micellar curcumin on clinical and laboratory parameters in patients with MS receiving low-efficacy DMTs (IFN-β). Methods: Sixty patients with MS and a suboptimal response to IFN-β were randomized (1:1) into two groups: the IFN-CUR group, which received add-on therapy with micellar curcumin (containing curcumin and Tween 80 as a solubilizer) for 6 months, and a control group (IFN group), which received IFN-β alone. The 6-month treatment period was followed by a subsequent 6-month follow-up off curcumin treatment (DMTs only). Results: The proportion of patients without relapses in the curcumin add-on group increased significantly after 6 months (from 57% to 90%, p = 0.007), and the risk of exacerbation was significantly lower compared to the control group (HR = 0.2; p = 0.03). The treatment was associated with EDSS score stabilization, a positive effect on depression (p = 0.05), and a reduction in plasma IFN-γ levels (p = 0.02). A decreasing trend in MRI lesion activity and reductions in specific microbiota-related markers, including the Eggerthella lenta-associated marker (i16a), were also observed. In ex vivo cultures, curcumin significantly inhibited IL-6 production in macrophages derived from patients with multiple sclerosis (MS) and healthy donors. Conclusions: Add-on therapy with micellar curcumin may enhance the efficacy of IFN-β, improving clinical outcomes and modulating inflammatory and microbial parameters in MS patients with a suboptimal response to IFN-β treatment. Full article

Human parainfluenza virus type 4 (hPIV4) remains poorly characterized compared with other hPIV serotypes and information on its genomic diversity is particularly limited for Russia and Eastern Europe. In this study, we report the first complete genome sequences of hPIV4 isolates from Russia and place them in the context of global hPIV4 genetic diversity. Eight hPIV4 viruses were isolated in cell culture from respiratory samples collected from hospitalized children in Saint Petersburg between 2017/2018 and 2023/2024. Complete viral genomes were recovered using a metagenomic whole-genome amplification approach based on SMART-9N technology. Phylogenetic analysis of 178 complete hPIV4 genomes showed clear separation into hPIV4a (n = 132) and hPIV4b (n = 46) subtypes. Based on genetic distance approach, hPIV4a formed two major clusters, with the dominant cluster B subdivided into four subclusters (B1–B4); and subcluster B4 further resolved into four genetic lineages. All Russian isolates belonged to the subcluster B4 and were distributed among multiple co-circulating lineages. In contrast, hPIV4b genomes segregated into three distinct clusters, reflecting structured genetic diversity within the subtype. Collectively, this study provides, to the best of our knowledge, the first p-distance-based framework for hPIV4 whole-genome classification and contributes new complete genome sequences for an underrepresented region. Full article

This paper presents a dataset collected through a visitor questionnaire survey conducted at the Archaeological Site of Ancient Dodona, Greece, a large-scale, spatially complex open-air archaeological site. The dataset documents visitors’ experiences, perceptions, and information needs, as well as their attitudes toward the use of digital technologies for heritage interpretation and engagement. The questionnaire was administered in printed form to adult visitors at the entrance and exit of the archaeological site. A total of 99 valid responses were collected. The dataset includes information on visitor demographics, visit characteristics, perceptions of existing interpretive material, spatial behavior within the site, and attitudes toward digital applications such as augmented reality, digital storytelling, and interactive tools. All data are fully anonymized and contain no personally identifiable or sensitive information. The dataset supports research in the fields of visitor studies, cultural heritage interpretation, digital heritage, and cultural tourism, and may be reused for comparative studies or for the design and evaluation of digital mediation applications in archaeological contexts. The dataset enables cross-tabulation analyses exploring associations between visitor characteristics and attitudes toward digital mediation, thereby supporting visitor segmentation and the evidence-based development of digital interpretation strategies in archaeological contexts. Full article

Generational amnesia is a common phenomenon in the religious realm. While we inherit established forms of belief, symbols, and ritual traditions, we often lack an understanding of their origins or how they came to be. In the ethnically diverse environment of Southeast Asia, certain cross-cultural connections may also disappear over time. This study seeks to highlight one goddess under the rubric of Chao Mae Thapthim—Waterfront Lady, within a Thai context. Chinese studies have regarded Waterfront Lady as a native Hainanese deity. However, this view fails to resolve the long-standing question concerning historical distribution of the belief across Hainan Island and Chinese communities in Southeast Asia. It also overlooks the internal connections between her and other goddesses referred to as Chao Mae Thapthim, such as the Heavenly Empress Mazu and some local female deities. I will begin with the discussion of the aforementioned issues and proceed to outline a probable cultural ‘worship sphere’ of Waterfront Lady in Thailand. Through this, I aim to present a case of intra-Asian religious interaction and syncretism. Full article

This study evaluated whether three-dimensional alginate–gelatin hydrogels (AGHs) crosslinked with calcium chloride (CaCl₂) enhance the osteo-odontogenic differentiation of odontoblast-like cells in vitro. Two seeding configurations were compared: inter-hydrogel (INT) surface seeding and intra-hydrogel (INTR) encapsulation. Here, the MDPC-23 cells were cultured in AGHs crosslinked with 70 or 100 mM CaCl₂ and assessed for proliferation, cytoskeletal morphology, alkaline phosphatase (ALPase) activity, osteo-odontogenic gene expression, and mineralized nodule formation. After 7 days, cell proliferation was significantly greater in the alginate–gelatin hydrogel (AGH) groups than in the control group. Cells in the intra alginate–gelatin hydrogel 100 (INTR-AGH100) remained predominantly rounded, whereas those in the inter alginate–gelatin hydrogel 100 (INT-AGH100) formed irregular clusters on the hydrogel surface. ALPase activity was highest in INTR-AGH100 at the early stage of culture. Both INT-AGH100 and INTR-AGH100 showed significantly increased expression of DSPP, DMP-1, BSP, OCN, OPN, and Runx-2, together with enhanced mineralized nodule formation. Although no significant differences were detected between the two seeding strategies in all assays, distinct morphological patterns were observed, and the INTR configuration showed relatively greater early differentiation-related activity. These findings suggest that 100 mM CaCl₂-crosslinked AGHs provide a favorable three-dimensional microenvironment under the present experimental conditions and represent a promising in vitro scaffold platform to support future studies of scaffold-guided dentin regeneration. Full article

This study addresses the critical challenge of translating the profound social, spatial, and cultural dynamics of the traditional introverted Persian house into more tangible design metrics for contemporary Iranian housing. Relying on qualitative data from twenty-four diverse expert interviews across architecture, urban planning, and policy, the research demonstrates a broad consensus that the notion of replicating historical form is unsustainable. Instead, it indicates that the introverted configuration is likely a context-specific ontological imperative—viewed here as a fundamental socio-spatial requirement—rooted in measurable performance, serving simultaneous social, cultural, psychological, and environmental paradigms. The main findings show that preserving cultural continuity requires a shift from aesthetic conservation to prescriptive configuration. This logic is synthesised into a consolidated socio-spatial framework, whose originality lies in introducing three regulatory design instruments: (1) the sequenced depth and filtration protocol for spatial arrangement; (2) the controlled visual and environmental parameters for façade performance; and (3) the cultural adaptability and resilience requirement for functional programming. The framework’s prescriptive metrics, such as minimum space syntax values and the visual filtering coefficient, provide regulatory bodies with the precise technical tools necessary to enforce cultural protocols like privacy and dignity in high-density urban developments. While these metrics serve as an operationally promising model, they represent a theoretical framework that requires further empirical validation in diverse contemporary residential settings before mandatory regulatory adoption. This framework offers a pragmatic pathway for safeguarding Iranian housing’s cultural identity, ensuring future developments are certified not only for safety and structure, but for their adherence to the fundamental socio-spatial contract of the Persian dwelling. Full article

Claude de Visdelou, a French Jesuit missionary who arrived in China in the 17th century, was renowned for his remarkable linguistic talent and profound knowledge of Sinology. He left behind numerous Latin translations of Chinese classics, many of which were preserved in manuscript form and are currently held in Biblioteca Apostolica Vaticana. Based on an examination of Visdelou’s life and his Latin translations of Confucian documents, such as Daxue, this paper aims to analyze the complex reasons why Visdelou openly opposed the Jesuit policy of tolerance toward Chinese rituals and was promoted by the Roman Curia for his opposition to the Jesuit’s approach. The paper also reflects on his translation activities as a personal intellectual struggle and as a means of cross-cultural knowledge construction from the perspective of Sino-Western cultural exchange history. Full article

This study examines how first-year Initial Teacher Education (ITE) students navigate early career development through critical turning points, the articulation of personal values, and the development of resilience. While teacher identity and professional formation have been explored conceptually, there remains limited empirical insight into students’ lived experiences at this formative stage. Adopting a qualitative design, in-depth interviews were conducted with 21 first-year ITE students from three UK universities, representing diverse cultural backgrounds and entry pathways, at the end of their first academic year. Data were analysed using a general inductive approach. Four interconnected themes emerged: clarifying purpose through critical turning points, negotiating tensions between personal values and institutional expectations, building resilience through community and reflective practice, and articulating meaning through spiritual perspectives in early teacher development. The findings demonstrate that formative experiences are not isolated events but are embedded within broader developmental trajectories shaped by relational support and opportunities for meaning-making. The study contributes to wider debates on early professional identity formation by offering an integrated, empirically grounded account of how purpose, values, and resilience interact to shape sustainable career pathways. Implications are discussed for ITE programme design and for supporting early-career development in the teaching profession and beyond. Full article

This article advances a critical rethinking of digital transformation in craft-based and socially embedded production systems by examining ateliers of social integration as community-led solidarity spaces where sewing and embroidery practices intersect with relational, care-oriented, and collective dimensions. Existing debates on digitalisation remain largely centred on automation, scale, and efficiency, overlooking how technology operates within care-based and territorially embedded economies. To address this gap, the article develops an alternative analytical framework grounded in relational economies and the ethics of care. While the phenomenon is transnational, the empirical analysis focuses on the Italian context and draws on data from CreAbility, an ongoing action-research project aimed at building a digital community of micro and small fashion enterprises, associations, and designers characterized by social and cultural impact. Against dominant, scale-oriented models of innovation, the article conceptualises ateliers of social integration as relational ecosystems in which value is co-produced through social ties, inclusion practices, and localized knowledge. From this perspective, digital technologies serve as situated mediators that extend and amplify proximity-based relations. This reframing challenges linear and growth-centred accounts of digital innovation, instead proposing a non-linear, care-centred, and place-based model of digital transformation. Methodologically, the study adopts a mixed-methods design combining quantitative and qualitative techniques. Data were collected between June and July 2025 through an online questionnaire distributed to a broader population of Italian ateliers of social integration and were complemented by participatory focus groups involving organisational representatives. The findings show that these ateliers operate as infrastructures of proximity in which production, care, and community are co-constitutive, and where digital practices support forms of extended embeddedness rather than substitution. In doing so, the article contributes to debates on digitalisation, social innovation, and the care economy by showing how alternative, relational, and non-scalable models of production can reshape the meaning and the trajectories of innovation. Full article

Chondroitin sulfate (CS) chains are major components of the extra- and pericellular matrix in the central nervous system (CNS), and their sulfation patterns influence CNS development and function. Highly sulfated CS preparations, including CS-D- and CS-E-enriched forms, have been shown to facilitate neurite outgrowth in cultured mouse hippocampal neurons. Notably, neurons cultured on CS-D- or CS-E-enriched substrates exhibited the following distinct morphological characteristics: CS-D promoted the extension of multiple short neurites, whereas CS-E induced the formation of a single elongated neurite with a polarization-like morphology. These features are consistent with early stages of neuronal polarization. However, the specific roles of these highly sulfated CS forms in polarization-like morphology remain unclear. In this study, we demonstrate that polarization-like morphological transitions in hippocampal neurons can be modulated on mixed CS-D/CS-E substrates by varying their ratios. Compared with CS-D-enriched substrates, CS-E-enriched substrates more effectively promoted polarization-like neuronal morphology, accompanied by enhanced activation of Src-family kinases. Furthermore, forced activation of Fyn kinase induced morphological changes resembling polarization-like features in a neuroblastoma cell line, even in the absence of CS-D/CS-E mixed substrates. In conclusion, highly sulfated CS subtypes may function as extracellular cues that regulate neuronal morphology via Src-family signaling, with likely involvement of Fyn. Full article

Growth media for human cell culture were developed in the twentieth century, when the first immortal human cell lines were established. The nutrient compositions of these media arose not from a desire to reproduce the microenvironment of the cells in vivo, but rather to encourage continuous replicative growth. Armed with comprehensive datasets detailing the metabolomes of the various fluid compartments within which cells reside, cell culturists are now exploring the effects of media designed to reproduce the in vivo environment on cell biology. The early results of this research indicate the media composition has profound impacts on cell form and function. In parallel, taking care to maintain oxygen at the relatively low levels found in vivo also affects many cellular activities. The lessons learned from ‘physiological cell culture’ should be applied to the culture of human embryos in the in vitro fertilization (IVF) clinic, where a critical stage of growth and development might be best supported by recreating, to the greatest extent possible, the environment of the oviduct and uterus. In this review, we translate recent advancements in physiological cell culture to emerging approaches in human embryo culture. Full article