Search Results (715)

In this study, polysaccharides from Ficus carica L. fruits (FCPs) were extracted using a deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) method. The physicochemical properties of the FCPs were then characterized, and the anti-aging effects of FCPs were evaluated in Caenorhabditis elegans (C. elegans). It was demonstrated that FCPs significantly extended the lifespan of the nematodes, while improving locomotor activity without affecting the body size or reproductive capacity. Meanwhile, FCPs reduced lipofuscin accumulation, decreased intracellular reactive oxygen species (ROS) levels, and increased the survival of C. elegans under oxidative stress. Moreover, FCPs upregulated the expression of antioxidant genes sod-1, sod-3, ctl-2, ctl-3 and gst-4. The expression of skinhead-1 (skn-1), a homologue gene of mammalian nuclear factor erythroid 2-related factor (Nrf) in C. elegans, was also elevated upon FCPs treatment. Knockdown of skn-1 expression by RNA interference abolished the lifespan extension and ROS reduction in FCPs-treated C. elegans, indicating that the SKN-1-mediated signaling was essential for the anti-aging effects of FCPs. Additionally, FCPs caused downregulation of the key components of the insulin/IGF-1 signaling (IIS) pathway, age-1, akt-1, and akt-2. Overall, these results suggested that FCPs promoted longevity in C. elegans via modulation of SKN-1 and IIS pathway. Full article

The environmental performance of chitosan production is evaluated through a rigorous computational comparison between traditional thermochemical deacetylation and innovative green synthesis pathways utilizing Deep Eutectic Solvents (DES). Implementation of the Waste Reduction (WAR) algorithm facilitates the quantification of the Potential Environmental Impact (PEI) across eight toxicological and ecotoxicological categories, providing a systematic benchmark for process sustainability. While the conventional route, characterized by the intensive consumption of HCl and NaOH, generates a substantial environmental burden of 1.37 × 10⁷ PEI/ton, the optimized green architecture leveraging a choline chloride:ethylene glycol (ChCl:EG) system achieves a radical reduction to 2.25 × 10⁴ PEI/ton. This 99.8% decrease in PEI is primarily driven by the mitigation of Human Toxicity Potential (HTP) and Acidification Potential (AP) through the substitution of corrosive mineral acids and volatile organics with biodegradable, low-vapor-pressure alternatives. These findings substantiate the integration of DES-mediated biorefineries as a high-efficiency strategy for the valorization of marine waste into high-purity biopolymers, aligning with the requirements for industrial process intensification and circular bioeconomy standards. Full article

In this study, the methane yield, substance conversion rate and microbial community structure of individual components of lignocellulose, synthetic mixtures, and corn straw subjected to different pretreatments (thermal hydrolysis, chemical, biological, and combined pretreatment) during anaerobic digestion were comparatively investigated. The synthetic mixture of cellulose and hemicellulose (MCXY) exerted a positive promoting effect on biomethane production, with a synergistic effect index of 101.51%. The methane yield per volatile solids (VS) of microcrystalline cellulose (MC), xylan (XY), and MCXY reached 320.81 ± 1.85 mL/g VS, 352.70 ± 6.58 mL/g VS, and 340.60 ± 10.94 mL/g VS, respectively. Lignin did not produce biogas in anaerobic digestion (AD) system, and its presence had an inhibitory effect on the methanogenesis of cellulose and hemicellulose, especially that of hemicellulose. Notably, pretreatment significantly improved the methane production potential of corn stover. Deep eutectic solvent-pretreated corn stover (DES_CS) achieved the highest methane yield of 356.57 ± 8.50 mL/g VS, which was 55.46% higher than that of the untreated group. DES pretreatment deconstructed lignocellulosic matrix and distinctly increased DOM molecular diversity, thus providing superior substrate conditions for improving anaerobic digestion performance. Microbial community analysis revealed that DES pretreatment significantly reshaped the bacterial structure, enriching syntrophic taxa over the carbohydrate-degrading Bacteroides found in raw corn stover, thereby fostering a more robust metabolic network for methane production. While acetoclastic Methanothrix dominated the pretreated groups, its synergistic coexistence with hydrogenotrophic Methanobacterium across all digesters facilitated stable dual-pathway methanogenesis. This work can provide a theoretical basis and technical reference for the optimization and application of pretreatment strategies for efficient anaerobic digestion of corn stover. Full article

Black garlic peels (BGPs) are a largely underutilized by-product despite representing an untapped source of bioactive compounds. This study presents a sustainable upcycling protocol for black garlic peels, evaluating natural deep eutectic solvents (NaDES) to develop multifunctional extracts for green cosmetics. Following the screening of four eutectic mixtures, the choline chloride–lactic acid (ChCl:LA) system demonstrated the highest extraction efficiency. The optimized extract yielded a remarkable total phenolic content (5216.61 µg GAE/mL) and strong antioxidant capacity, confirmed by DPPH and FRAP assays, associated with recovering both free and bound phenolic fractions. Subsequent HPLC profiling characterized the extract, and the comparative analysis explicitly demonstrated that antimicrobial activity is entirely driven by and identical to the pure eutectic solvent vehicle rather than the extracted garlic biomass, with broad-spectrum efficacy against C. albicans, P. aeruginosa, and S. aureus. To evaluate its cosmeceutical potential, the extract was incorporated into emulsions (5%, 10%, 15% w/w) with inorganic or organic UV filters. Although the direct Sun Protection Factor (SPF) and the UVA Protection Factor (UVA-PF) did not show enhancing results, a photochemiluminescence (PCL) analysis revealed a synergistic behaviour with organic filters, successfully boosting the formulation’s biological antioxidant shield. This pioneering work highlights BGP’s upcycling potential, proposing NaDES extracts as highly promising multifunctional, antioxidant, and antimicrobial ingredients for next-generation cosmeceuticals. Full article

The present study investigated the use of natural deep eutectic solvents (NaDESs) combined with ultrasound-assisted extraction (UAE) for the efficient recovery of anthocyanins and antioxidant phenolics from Damask rose (DR). A wide range of environmentally friendly solvents was screened, and choline chloride–propylene glycol (ChCl-PG) was identified as the most effective extraction medium. The extraction conditions were optimized using response surface methodology (RSM) and artificial neural network (ANN) models to maximize anthocyanin and phenolic contents, as well as antioxidant activity. Under the optimal parameters, the DR extracts exhibited relatively high levels of bioactive compounds, including total anthocyanin content of 5.2–5.3 mg cyanidin-3-glucoside equivalents g⁻¹ sample, total phenolic content of 63.4–64.2 mg gallic acid equivalents g⁻¹ sample, along with substantial antioxidant potential (DPPH: 68.2–68.8% inhibition, FRAP: 581.6–591.9 μmol Trolox equivalents g⁻¹ sample). Chromatographic analysis of the optimum extract revealed cyanidin as the predominant anthocyanidin in DR, and its stability was further evaluated, revealing improved preservation under dark conditions at lower temperatures over a 15-day storage period. Moreover, the IC₅₀ values confirmed antimicrobial effects against the tested foodborne pathogens. Furthermore, the inhibitory effect of the DR extract remained stable against S. aureus and S. cerevisiae throughout the storage period. Overall, the findings demonstrate that NaDES-UAE is a promising and sustainable approach for obtaining anthocyanin-rich DR extracts with antioxidant and antimicrobial potential. Full article

This study investigated the influence of solvent composition on the extraction efficiency and selectivity of phenolic compounds from the Amazonian fruits açaí (Euterpe oleracea) and bacaba (Oenocarpus bacaba). Six choline chloride-based natural deep eutectic solvents (NaDES), combined with different hydrogen bond donors (glycerol, 1,2-propanediol, citric acid, lactic acid, oxalic acid, and urea), were compared with acidified methanol. Extracts were evaluated for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity using FRAP, DPPH, and ORAC assays. In açaí, methanol exhibited the highest TPC and reducing capacity, whereas acid-based NaDESs enhanced phenolic recovery in a matrix-dependent manner. In bacaba, choline chloride–citric acid enhanced total phenolic recovery compared to methanol, highlighting matrix-dependent solvent performance. Differences among FRAP, DPPH, and ORAC responses reflected variations in phenolic composition rather than total concentration alone. HPLC-DAD analysis revealed solvent-selective enrichment of anthocyanins, chlorogenic acid, flavan-3-ols, and rutin, particularly with acid-based NaDES formulations. Molecular docking provided complementary mechanistic insight by indicating favorable interactions between major phenolics and polyphenol oxidase. Overall, the results indicate that choline chloride-based NaDESs can function as tunable extraction systems capable of modulating phenolic profiles in a matrix-dependent manner, representing promising alternatives to conventional organic solvents for phenolic recovery from Amazonian fruits. Full article

The fresh-cut avocado processing generates significant amounts of by-products, mainly peel and seed, with the peel representing a valuable source of phenolic compounds. In this context, the growing demand for sustainable technologies encourages the use of green solvents for bioactive compound recovery. In this study, natural deep eutectic solvents (NaDES) were evaluated as environmentally friendly solvents for the extraction of phenolic compounds from Hass avocado peels through ultrasound-assisted extraction and for their potential application in fresh-cut avocado. Phenolics were extracted using acidic water, ethanol, and NaDES based on choline chloride as a fixed hydrogen bond acceptor (HBA) and hydrogen bond donors (HBDs; lactic acid, glycerol, and citric acid) with the ultrasound-assisted system. The stability of the extracts was monitored for eight weeks (four weeks in darkness followed by four weeks under light exposure). Among the tested formulations, the lactic-acid-based NaDES showed the highest extraction efficiency and the best stability of phenolic compounds during storage (≥20 mg GAE g⁻¹ dw during the storage period). The lactic-acid-based extract was then applied to fresh-cut avocado to evaluate its potential for antioxidant enrichment and browning prevention during refrigerated storage. The treatment increased phenolic content and contributed to improved color stability (during seven days of storage). Overall, lactic-acid-based NaDES represent a promising green solvent system for recovering phenolics from avocado peel and for their functional application in fresh-cut avocado within a circular valorization approach. Full article

Spent coffee grounds (SCGs), a major by-product of coffee consumption, remain an underused source of chlorogenic acid (CGA) and other phenolic constituents. This study investigated an ultrasound-assisted extraction strategy using deep eutectic solvents (DESs) to improve the recovery and phenolic-acid enrichment of SCGs. Among the tested DES formulations, the betaine–acetic acid system gave the best CGA extraction performance and was therefore used for further optimization by response surface methodology. The optimized process, conducted at a liquid-to-solid ratio of 28 mL/g, 75 °C, and 50 min, produced a CGA yield of 15.18 mg CGA/g dried SCG powder, markedly exceeding that achieved with 70% ethanol under comparable conditions. Structural and chemical characterizations helped explain this improvement. Scanning electron microscopy revealed that the DES-based process caused more evident disruption of the SCG matrix, which favored solvent penetration and mass transfer. Fourier-transform infrared spectroscopy confirmed the formation of a hydrogen-bonding network between betaine and acetic acid. Ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry further revealed that the betaine–acetic acid extract was mainly composed of CGA and hydroxycinnamic acid derivatives. The purified extract also displayed strong in vitro antioxidant capacity. Overall, the betaine–acetic acid DES combined with ultrasound provides an effective green approach for recovering CGA-rich phenolic extracts from SCGs. Full article

Hydrothermal carbonization (HTC) has emerged as a promising technique for food waste treatment. However, food waste is composed of complex components, including refractory proteins and polysaccharides, which lead to low efficiency and high costs during the HTC process. Enhancing the decomposition of food waste while enabling efficient nutrient recovery remains a significant challenge for the widespread application of HTC in food waste management. This study introduces deep eutectic solvents (DESs) to enhance treatment efficiency during the HTC of food waste. A comprehensive characterization of the resulting hydrochar and aqueous phase was conducted, and the effect of DES addition on the migration and speciation of phosphorus and nitrogen species during HTC was investigated. The results indicated that the addition of DESs promoted the decomposition of food waste, reducing the hydrochar yield from 22.6% to 20.2% and decreasing the volatile matter content in the hydrochar from 86.63% to 71.60% at 200 °C. Additionally, DESs significantly lowered the nitrogen content in the hydrochar from 5.99% to 3.77%. By disrupting the hydrogen-bonding networks in proteins and polysaccharides, DESs facilitated the dissolution of organic matter into the aqueous phase. Furthermore, with DES addition, 5.06 mg of phosphorus species was enriched in the hydrochar, compared to only 1.78 mg in the control group without DESs. This study provides a sustainable strategy for the efficient treatment of food waste while simultaneously enabling the effective recovery of valuable nutrients. Full article

Hydrogen represents a promising clean and renewable energy source. Therefore, improving the efficiency of electrocatalysts is essential for effective hydrogen production. In this work, Ni electrocatalysts were synthesized via the electrodeposition method from ethaline deep eutectic solvent (DES) at 45 °C, 55 °C, and 65 °C under perpendicular (B_⊥) and parallel (B_‖) magnetic field directions relative to the electrode surface. Scanning electron microscopy (SEM) was employed to investigate the morphological study, which shows that Ni deposits under B_⊥ promote columnar grain growth, while B_‖ favors lateral, compact structures. Furthermore, moderate temperature (55 °C) in the case of using B_⊥ produced finer grains and smoother surfaces compared to other temperatures in the same direction, enhancing the catalytic performance for HER. Electrochemical techniques, including linear sweep voltammetry (LSV) and chronoamperometry, were employed to evaluate the catalytic performance for HER in 1 M NaOH and the adsorption–desorption process, respectively. The results suggest that efficient HER performance is associated with balanced hydrogen adsorption and desorption behavior. The Ni deposit at 55 °C under (B_⊥) exhibited the lowest overpotential (−215 mV) compared to the deposits at 45 °C and 65 °C under the same magnetic field direction, indicating superior overall HER performance. This performance is attributed to balanced hydrogen adsorption–desorption behavior despite the relatively high Tafel slope value (298 mV·dec⁻¹). However, the lowest Tafel slope among the whole samples prepared under both (B_⊥) and (B_‖) was found to be (219 mV·dec⁻¹), reflecting faster kinetics, which was obtained for the sample deposited at 45 °C under (B_‖). Full article

Biomass represents a vital and sustainable resource for developing renewable materials with the potential to replace petroleum-based chemicals. Paulownia wood has high cellulose content and a loose wood structure, giving it natural advantages as a biomass material. Therefore, in this study, Paulownia wood was selected as a lignocellulosic feedstock. An integrated pretreatment process combining a deep eutectic solvent (DES) with an organic solvent was employed to efficiently remove lignin and hemicellulose, yielding cellulose-enriched residues. Subsequently, high-intensity ultrasonication was applied to convert the residues into cellulose nanofibers and nanocrystals. Using the extracted cellulose and nanocellulose, a dual-crosslinked network composite hydrogel was fabricated. The structural, mechanical, thermal, swelling, and conductive properties of the hydrogel were systematically investigated. The results show that, compared with the blank group hydrogel, the addition of nanocellulose increased the maximum tensile strength and tensile strain of the composite hydrogel by approximately 113% and 81%, respectively; meanwhile, the compressive strengths of the nanocellulose-based hydrogels (0.04575–0.09060 MPa) are higher than that of the blank group hydrogel (0.04235 MPa), confirming that the incorporation of nanocellulose significantly enhances the mechanical strength and elasticity of the hydrogel. The introduction of an AlCl₃/ZnCl₂ solvent system imparts appreciable electrical conductivity. Furthermore, the composite hydrogel maintains structural integrity after full swelling, indicating good dimensional stability and reusability. This work not only presents a green and efficient strategy for valorizing Paulownia biomass but also offers a novel design route for high-performance conductive hydrogel materials, highlighting their potential application in areas such as flexible electronics and energy storage. Full article

Polylactic acid (PLA), a biodegradable polymer derived from renewable resources, represents a promising candidate for sustainable textiles. Nevertheless, its practical application remains limited by the requirement for high-temperature dyeing, which can induce polymer hydrolysis and lead to the loss of fiber strength. To address this limitation, PLA fabric was treated with an eco-friendly natural deep eutectic solvent (NaDES) composed of glycerol and citric acid. The treatment was found to enhance fiber surface roughness and internal looseness, which facilitated dye diffusion and allowed for a significant reduction in dyeing temperature. When dyed with microbial prodigiosin, the treated PLA fabric achieved a color depth at 70 °C that was equivalent to untreated fabric at 90 °C, while also exhibiting a 93.56% bacteriostatic rate against Staphylococcus aureus due to the inherent antibacterial property of microbial prodigiosin. This work provides a novel and sustainable strategy for the eco-friendly dyeing of PLA textiles. Full article

Over the last 20 years, Deep-Eutectic Solvents (DES) have been making a significant impact in the field of chemistry, with applications in nanotechnology, biomass transformation, electrochemistry pharmaceuticals and a host of other applications that includes catalysis. Considering the importance of chiral organocatalysis for the selective synthesis of drugs, pharmaceuticals and fragrances, etc. DESs were quickly harnessed as the media for carrying out organocatalytic transformations. In this review, we discuss some of the most important examples from the literature that have made an impact in the field over the last 5 years. A more recent development has been the incorporation of DESs in structured and self-organized gel-like assemblies that are known as EutectoGels. These soft structures offer a more defined and compact environment that can influence stereoselectivity by pre-organizing the reactants in three-dimensional space, and potential control the types of transition states that can be formed. Full article

(1) Background: Carvedilol is a poorly water-soluble drug, which limits its therapeutic performance. Deep eutectic solvents (DES) and cyclodextrins (CD) are emerging solubilizing agents that can improve drug bioavailability. (2) Methods: Twenty-one DES were prepared using choline chloride and polyols or sugars (xylitol, sorbitol, glucose, and fructose) at different molar ratios with water. α and β cyclodextrins (CD) were added (0.5–2 mM) using two incorporation strategies: (Method 1) addition to the aqueous phase before DES formation; (Method 2) direct addition to the preformed DES. (3) Results: Carvedilol solubility markedly increased with DES–CD combinations. In Method 1, xylitol-based DES provided up to a 16-fold enhancement, especially with β-CD at low concentrations, while glucose and sorbitol systems showed modest effects. Fructose-based mixtures improved mainly at a 2:1:35 ratio without CDs. In Method 2, α-CD with xylitol or sorbitol yielded the highest increases (up to 38.9-fold). (4) Conclusions: The solubilization efficiency depends on DES composition, CD type, and concentration. α-CD combined with xylitol-based DES showed the best results, highlighting this approach as a promising strategy to enhance carvedilol solubility for pharmaceutical applications. Full article

Deep eutectic solvents (DESs) have emerged as powerful media for enhancing the solubility of poorly water-soluble active pharmaceutical ingredients (APIs). However, their rational design remains challenging due to the complex interplay of intermolecular interactions and non-ideal thermodynamic behavior. This study develops a comprehensive, data-driven taxonomy of solute–solvent systems by integrating COSMO-RS-derived descriptors with principal component analysis (PCA) and unsupervised clustering. This approach establishes a constrained, evidence-based decision framework, which is more appropriate for complex physicochemical systems like DESs than traditional empirical rules. The analysis successfully reduces the multidimensional descriptor space to five physically interpretable axes: solvation driving force, API thermodynamic stability, solvent interaction profile, hydrogen-bond network strength, and hydration effects. Two primary solubilization mechanisms are identified: interaction-driven solvation, characterized by high API–DES affinity, and destabilization-driven solvation. Furthermore, comparison of dry and water-containing systems reveals that water acts as a thermodynamic structuring agent, fundamentally reducing system dimensionality and promoting the emergence of more distinct solvation regimes. Validated through the projection of benzocaine and lidocaine, this framework enables a transition from trial-and-error screening to mechanism-guided formulation design, providing a robust roadmap for navigating the complex solubility landscape of pharmaceutical DESs. Full article