Search Results (844)

Pistachio (Pistacia vera L.) holds significant importance due to its diverse applications and nutritional benefits. The nuts are rich in essential amino acids, antioxidants, fiber, healthy fats, and minerals, making them highly valuable for human nutrition. However, pistachios are significantly challenged by salinity stress, which negatively affects their growth and metabolism. Understanding the impact of salinity stress on pistachios is crucial for developing effective strategies to enhance their tolerance, improve growth, and ensure sustainable production in saline environments. To investigate the effects of salinity on energy metabolism and amino acid composition, we monitored key metabolites and free amino acid levels in UCB-1 pistachio leaves at 7- and 21-day salt stress treatments using Liquid Chromatography–Mass Spectrometry (LC-MS) and Ultra Performance Liquid Chromatography (UPLC). Our findings revealed that salinity affected nearly all analyzed metabolites, with varied patterns observed at different time points. Notably, all free amino acids except threonine accumulated significantly in response to salt stress. Meanwhile, reductions in 3PGA, Fru1,6bP, and Glu6P+Fru6P (glycolysis and Calvin cycle intermediates) suggest a decrease in photosynthetic activity, which may ultimately impact respiration rates. These results demonstrate that salinity stress affects both amino acid metabolism and central carbon metabolism, with the magnitude and pattern of these changes depending on the duration of exposure. The observed metabolic adjustments likely represent an adaptive response, enabling the plant to partially mitigate the detrimental effects of salt stress. Full article

Electricity price spikes are the most important characteristic of the electricity price time series. Operationally, they result from various stresses in the power system or the strategic bidding behavior of market participants. These high prices are important as they represent economic opportunities in the form of profits and savings. Theoretically, price spikes are defined as prices that exceed a threshold over a typically short duration. This definition serves as the basis for several established modeling approaches in the literature. In general, the threshold component determines the design of a price spike model, often overlooking the duration aspect. Therefore, this paper presents a simple yet informative model to quantify the duration of electricity price spikes using historical price data from different market jurisdictions. We approach the problem through the lens of survival analysis, a widely used technique for evaluating time-to-event data. Specifically, we use the Kaplan–Meier (KM) estimator, which enables a nonparametric evaluation of the survival (duration) of price spikes over time. We refer to this as the price spike duration model. Full article

Posttraumatic Stress Disorder (PTSD) is characterized by disruptions in central nervous system functioning and existential crises, yet the mechanistic links between neurobiological processes and dimensions of life meaning and identity remain underexplored. The aim of this study was to examine the relationships between stress biomarkers (serotonin, cortisol, noradrenaline, and interleukin-12 [IL-12]) and existential attitudes (measured using the Life Attitude Profile (Revised) [LAP-R]) in mining rescuers, considering PTSD duration and participant age. This cross-sectional study included 92 men aged 18–50 years, divided into three groups: no PTSD (n = 28), PTSD ≤ 5 years (n = 33), and PTSD > 5 years (n = 31). Serum levels of four biomarkers and LAP-R scores across eight domains were evaluated. Statistical analyses employed nonparametric tests, including the Kruskal–Wallis test for overall group differences (with Wilcoxon r effect sizes for pairwise comparisons, Mann–Whitney U tests for post hoc pairwise comparisons, and Spearman’s rank correlations for biomarker–LAP-R associations. Age effects were assessed in two strata: 18–35 years and 36–50 years. Kruskal–Wallis tests revealed significant group differences (p < 0.001) for all biomarkers and most LAP-R domains, with very large effect sizes (r > 0.7) in pairwise comparisons for serotonin (control median: 225.2 ng/mL vs. PTSD ≤ 5y: 109.9 ng/mL, r = 0.86; vs. PTSD > 5y: 148.0 ng/mL, r = 0.86), IL-12 (control: ~8.0 pg/mL vs. PTSD ≤ 5y: 62.4 pg/mL, r = 0.86; vs. PTSD > 5y: ~21.0 pg/mL, r = 0.69), and LAP-R scales such as Life Purpose (control: 54.0 vs. PTSD ≤ 5y: 39.0, r = 0.78; vs. PTSD > 5y: 20.0, r = 0.86) and Coherence (control: 53.0 vs. PTSD ≤ 5y: 34.0, r = 0.85; vs. PTSD > 5y: 23.0, r = 0.86). The PTSD ≤ 5y group exhibited decreased serotonin, cortisol (median: 9.8 µg/dL), and noradrenaline (271.7 pg/mL) with elevated IL-12 (all p < 0.001 vs. control), alongside reduced LAP-R scores. The PTSD > 5y group showed elevated cortisol (median: ~50.0 µg/dL, p < 0.001 vs. control, r = 0.86) and normalized IL-12 but persistent LAP-R deficits. Older participants (36–50 years) in the PTSD ≤ 5y group displayed improved existential attitudes (e.g., Life Purpose: 47.0 vs. 27.5 in 18–35 years, p < 0.001), whereas in PTSD > 5y, age exacerbated biological stress (cortisol: 57.6 µg/dL vs. 36.1 µg/dL, p = 0.003). Spearman correlations revealed stage-specific patterns, such as negative associations between cortisol and Death Acceptance in PTSD > 5y (ρ = −0.49, p = 0.005). PTSD alters biomarker levels and their associations with existential dimensions, with duration and age modulating patient profiles. These findings underscore the necessity for integrated therapies addressing both biological and existential facets of PTSD. Full article

Background/Objectives: Allergic contact dermatitis (ACD) is a chronic inflammatory disease with a high prevalence, affecting various aspects of patients’ lives. Psychosocial factors may influence disease management and outcomes, including perceived stress, locus of control, and self-efficacy. This study examines the presence of these factors in ACD and their association with disease severity and patient characteristics. Methods: A cross-sectional study included 225 adults with ACD and 225 healthy controls. Exclusion criteria were other skin diseases, psychiatric disorders, or intellectual disabilities. Sociodemographic and clinical variables, such as disease duration and severity, were recorded. Perceived stress, locus of control, and self-efficacy were assessed using validated questionnaires. Statistical analyses, including t-tests and multiple linear regression, were performed to explore group differences and predictors of clinical and psychosocial outcomes. Results: ACD patients exhibited higher perceived stress than controls (M = 39.36 vs. 24.74, p < 0.001), with stress levels correlating with disease severity (B = 0.062, 95% CI [0.050, 0.074], p < 0.001). Female sex (B = −5.896, p < 0.001) and lower education (B = −2.606, p = 0.035) predicted higher stress. Locus of control and self-efficacy showed statistically significant but modest differences between groups. Conclusions: Perceived stress was significantly associated with the severity of ACD, highlighting the necessity of incorporating psychological interventions into disease management. Programs focused on stress reduction and patient education should be integrated into clinical care to enhance outcomes. Longitudinal research is essential to establish causal relationships and evaluate the long-term benefits of tailored psychological support on disease progression and patient well-being. Full article

High-energy (710 MeV) Bi ion track morphology in polycrystalline silicon nitride was investigated during post-irradiation annealing. Using both in-situ and ex-situ transmission electron microscopy, we monitored the recovery of crystallinity within initially amorphous ion track regions. In-situ annealing involved heating samples from room temperature to 1000 °C in 50 °C increments, each held for 10 s. We observed a steady decrease in both the size and number of tracks, with only a small number of residual crystalline defects remaining at 1000 °C. Ex-situ annealing experiments were conducted at 400 °C, 700 °C, and 1000 °C for durations of 10, 20, and 30 min. Complete restoration of the crystalline lattice occurred after 30 min at 700 °C and 20 min at 1000 °C. Due to inherent differences in geometry, heat flow, and stress conditions between thin lamella and bulk specimens, in-situ and ex-situ results cannot be compared. Molecular dynamics simulations further revealed that track shrinkage begins in cells within picoseconds, supporting the notion that recrystallization can start on very short timescales. Overall, these findings demonstrate that thermal recrystallization of damage induced by swift heavy ion irradiation in polycrystalline Si₃N₄ is possible. This study provides a foundation for future research aimed at better understanding radiation damage recovery in this material. Full article

Microgravity exposure during spaceflight has been linked to cognitive impairments, including deficits in attention, executive function, and spatial memory. Both space missions and ground-based analogs—such as head-down bed rest, dry immersion, and hindlimb unloading—consistently demonstrate that altered gravity disrupts brain structure and neural plasticity. Neuroimaging data reveal significant changes in brain morphology, functional connectivity, and cerebrospinal fluid dynamics. At the cellular level, simulated microgravity impairs synaptic plasticity, alters dendritic spine architecture, and compromises neurotransmitter release. These changes are accompanied by dysregulation of neuroendocrine signaling, decreased expression of neurotrophic factors, and activation of oxidative stress and neuroinflammatory pathways. Molecular and omics-level analyses further point to mitochondrial dysfunction and disruptions in key signaling cascades governing synaptic integrity, energy metabolism, and neuronal survival. Despite these advances, discrepancies across studies—due to differences in models, durations, and endpoints—limit mechanistic clarity and translational relevance. Human data remain scarce, emphasizing the need for standardized, longitudinal, and multimodal investigations. This review provides an integrated synthesis of current evidence on the cognitive and neurobiological effects of microgravity, spanning behavioral, structural, cellular, and molecular domains. By identifying consistent patterns and unresolved questions, we highlight critical targets for future research and the development of effective neuroprotective strategies for long-duration space missions. Full article

Surgical trauma triggers oxidative and inflammatory responses that contribute to postoperative complications. Although the antioxidant and anti-inflammatory effects of ketamine have been reported, the impact of anesthesia duration on these mechanisms remains unclear. Forty-two male Wistar rats were randomized into healthy control (HG), ketamine only (KET; 60 mg/kg, i.p.), or laparotomy plus ketamine with 0–4 additional ketamine doses at 20 min intervals (KET + L, KET + L1–L4). At 24 h, levels of MDA, tGSH, SOD, CAT, IL-1β, IL-6, TNF-α, adrenaline and noradrenaline were measured in tail-vein blood. One-way ANOVA with Tukey’s post hoc test was used. Laparotomy under single-dose ketamine increased MDA and pro-inflammatory cytokines and decreased tGSH, SOD, CAT, ADR, and NDR versus HG and KET (all p < 0.001). After laparotomy, repeated ketamine dosing produced graded decreases in MDA and cytokines and increases in tGSH, SOD, CAT, ADR, and NDR toward control levels; effects were most pronounced in KET + L4 (all p < 0.001). Ketamine alone did not differ significantly from HG. In rats, ketamine modulates postoperative biological stress in a duration-dependent manner; prolonging anesthesia reduces oxidative–inflammatory load and restores catecholaminergic tone. These findings strongly support revisiting dose–duration protocols and underscore the need for mechanistic and clinical studies. Full article

Endothelial dysfunction (characterized by reduced vasodilation or vasoconstriction, oxidative stress, inflammation, and pro-thrombotic condition) is a critical factor in the pathophysiology of various cardiovascular conditions, and the application of anesthetics can affect this dysfunction. Patients undergoing major surgery, especially cardiovascular surgery, are at increased risk of endothelial dysfunction. The impact of anesthetics on endothelial function can vary depending on the specific agent, dosage, duration of exposure, comorbidities, etc. Certain anesthetics, especially at higher doses, may increase the production of reactive oxygen species (ROS), leading to oxidative stress and endothelial dysfunction through reduced nitric oxid (NO) availability. Some anesthetics can modulate inflammatory responses, either by suppressing or exacerbating inflammation, or may affect the permeability of the endothelium, potentially leading to pulmonary edema and disruption of the blood-brain barrier. Anesthetics can influence endothelial glycocalyx. Understanding anesthetics effects is crucial for optimizing anesthetic management, particularly in patients with pre-existing cardiovascular issues. Therefore, the aim of this review is to critically evaluate the effects of different classes of anesthetics on endothelial function and oxidative stress. Specifically, we address how anesthetics influence NO bioavailability, endothelial glycocalyx integrity, inflammatory and oxidative pathways, and clinical outcomes in surgical patients. By summarizing current evidence, we aim to highlight mechanistic insights and identify potential perioperative strategies to minimize endothelial dysfunction. Full article

Photosystem II (PSII) is very sensitive to both biotic and abiotic stress conditions, mirroring global climate changes. Crop production worldwide faces rising hazards from the increased duration, frequency, and intensity of drought stress episodes as a result of climate change, and its effects, when combined with biotic stress, are becoming more noticeable. In the present work, we examined PSII responses of well-watered (WW) tomato plants or mildly drought-stressed (MDS) plants to 20 min of Tuta absoluta larvae feeding. The effective quantum yield of PSII photochemistry (Φ_PSII) of the whole leaf in WW plants, after 20 min of larvae feeding, compensated for the reduction in Φ_PSII observed at the feeding area. In contrast, the reduced Φ_PSII at the feeding areas of MDS plants, after 20 min of larvae feeding, was not compensated at the whole-leaf level because of the drought stress. The increased Φ_PSII and electron transport rate (ETR) at the whole-leaf level in WW plants was attributed to the increased fraction of open PSII reaction centers (qp), since there was no difference in the efficiency of the open PSII reaction centers (Fv′/Fm′) before and after feeding. Therefore, the response of PSII photochemistry in WW plants to short-term biotic stress resulted in an overcompensation reaction, which developed a whole-leaf photosynthetic enhancement. However, short-term biotic stress in combination with mild abiotic stress resulted in decreased PSII photochemistry. It is concluded that increased crop damage is likely to occur due to the global climate-change-induced drought episodes, influencing insect herbivory. Full article

Pelvis and trunk counter-rotation are key factors known to effect throwing arm kinematics in baseball pitching, where energy or momentum is transferred from the lower extremities through to the trunk during the pitching cycle. The purpose of this study was to retrospectively analyze previously recorded motion capture data of 19 skilled competitive pitchers to test the a priori hypothesis whether different stride lengths affect transverse pelvis and trunk biomechanics. A blinded randomized crossover design was used where pitchers threw two simulated games at ±25% from desired stride length (DSL), respective of overstride (OS) and under-stride (US). Variables of interest included pelvic–trunk separation (PTS) angle or degree of uncoupling and proximal plyometric effect (PPE) or ratio between trunk–pelvis angular velocities, as surrogate measures of rotational and elastic energy transfer. Paired t-tests were used to compare across stride conditions. A one-way ANOVA with a Bonferroni post hoc analysis demonstrated stride lengths differed statistically, (DSL vs. OS p = 0.006), (DSL vs. US, p < 0.001), and (US vs. OS, p < 0.001). Despite the statistically different stride lengths, fastball velocities tracked with radar were consistent. No significant differences within and across innings pitched between OS and OS conditions were found. The ±25% stride length changes influenced temporal parameters within the pitching cycle. Shorter stride elicited by early SFC reduced time during the Generation phase and extended the Brace-Transfer duration (p < 0.001). Statistically different transverse pelvis and trunk kinematics at hallmark events and phases consequently influenced pelvic–trunk separation and proximal plyometrics. During the Generation (PKH-SFC) and Brace-Transfer (SFC-MER) phases, the pelvis and trunk were significantly more externally rotated (p < 0.001) with shorter strides, concomitant with less separation at the instant of SFC and the Generation phase with greater peak proximal plyometrics effect ratios peak during throwing arm acceleration, indicative of greater contribution of trunk angular velocity (p < 0.05). Greater transverse trunk angular velocities relative to the pelvis late in double support necessitates the throwing arm to “catch up” from a position of greater arm lag, which compromises the dynamic and passive stabilizers. In conclusion, stride length alters pitching biomechanics and timing of peak pelvic–trunk separation and trunk angular velocity relative to the pelvis. Increased shoulder and elbow tensile stress is to be expected, consequently increasing risk for injury. Full article

Background/Objectives: Wheat bran is known for its anti-aging effects, primarily due to its antioxidant properties. Our previous study identified novel antioxidants in wheat bran (xylo-oligosaccharides and protein hydrolysates) using an innovative extraction method. However, the anti-aging potential of these wheat bran extracts (WBEs) remains unclear. Methods: This study evaluated the anti-aging effects of WBE in a D-galactose-induced aging model using Wistar rats. Animals were divided into four groups: (1) saline-injected control, (2) D-galactose-injected control, (3) D-galactose + 5% WBE, and (4) D-galactose + 10% WBE. After six weeks, body weight, food intake, body fat percentage, erythrocyte superoxide dismutase (SOD) activity, and liver senescence-associated β-galactosidase (SA-β-gal) levels were assessed. Results: D-galactose significantly reduced food intake in positive control 87 ± 21%/weekly (negative control; p < 0.05, 107 ± 20%/weekly for 10%WBE; p < 0.01. Body fat percentage (positive control: 84 ± 19% vs. 5% WBE: 110 ± 20%, p < 0.05 in 100% convert). It also lowered erythrocyte SOD activity; 68.6 ± 9%, p < 0.01 in 100% conversion). WBE supplementation restored SOD activity in a dose-dependent manner (5% WBE: 32,479 ± 12,773 U/mL; 10% WBE: 42,368 ± 20,281 U/mL. Although D-galactose did not elevate significantly SA-β-gal activity in the liver, WBE supplementation still led to a dose-dependent reduction in baseline SA-β-gal levels (294 ± 84 nmol/min/mg protein vs. 5% WBE: 181 ± 65 nmol/min/mg protein, and 10% WBE: 146 ± 40 nmol/min/mg protein. p < 0.001). No significant group differences were found in hepatic SOD2, catalase (liver and skin), or telomerase reverse transcriptase expression. Conclusions: These findings suggest that wheat bran extracts mitigate D-galactose-induced oxidative stress in circulation, indicating potential anti-aging benefits. However, their effects at the tissue level remain inconclusive. Further studies are needed to explore molecular mechanisms and refine intervention duration. Full article

While numerous bioactive polysaccharides have been identified from mushrooms, their mechanisms of action, particularly through the induction of oxidative stress in tumor cells, remain underexplored. This study isolates a novel polysaccharide, LSPS2, derived from Laetiporus sulphureus, followed by the elucidation of its distinctive structural features and specific antitumor activity in A549 lung carcinoma cells. LSPS2 was composed primarily of glucose (88.1%) and minor amounts of mannose (8.0%) and galactose (3.9%). Methylation and one-dimensional/two-dimensional nuclear magnetic resonance (1D/2D NMR) analysis results indicated that LSPS2 was composed of (1→3)-linked-D-β-glucopyran residues and (1→4)-linked-D-α-glucopyran residues as the main chain. The side chains were connected to O-6 and O-3 of glucopyranose (Glcp) residues with terminal Glcp. It differs from previous reports on L. sulphureus polysaccharides. Functionally, LSPS2 markedly suppressed A549 cell viability in a manner that depended on both exposure duration and concentration. LSPS2 upregulated malondialdehyde (MDA) and downregulated reduced glutathione (GSH), demonstrating that LSPS2 induces oxidative stress in A549 cells. The results of superoxide dismutase (SOD) activity assays further indicated that LSPS2 downregulates SOD activity, which may be the mechanism by which LSPS2 induces oxidative stress and, consequently, apoptosis in A549 cells. This targeted downregulation of a key antioxidant enzyme highlights a potential pathway for polysaccharide-induced tumor cell death. Our findings not only identify LSPS2 as a structurally distinct biopolymer but also elucidate its mode of action, underscoring its prospective application in tumor therapy and functional foods, warranting further investigation. Full article

Sclerodermus guani is the most extensively studied and widely used parasitoid wasp for controlling forest trunk-boring pests in China, with temperature being a key environmental factor affecting its development and reproduction. Against the backdrop of global climate change, the frequency of heat stress on parasitoid wasps is increasing. Therefore, studying their adaptability to heat stress is of great significance for breeding heat-tolerant strains. The findings of such research may help improve efficiency in rearing this wasp and provide guidance for its use in pest control. This study investigated the thermal adaptability of the wasp at temperatures ranging from 21 to 33 °C. The results showed that temperature significantly affected its parasitism capacity and behavior. As temperature increased, female wasps searched for and stung hosts more actively, and their pre-oviposition period decreased, as well as their developmental duration. The reproductive fitness of the wasp also varied significantly with temperature, with the most favorable temperatures being 27 °C and 30 °C. Both low and high temperatures were detrimental to its reproduction, and the difference in the number of female offspring was significant. The experimental results indicate that S. guani has higher reproductive fitness at 27–30 °C. Therefore, it is recommended that this parasitoid wasp be reared indoors at 27–30 °C and released for pest control in forests when the temperature is above 24 °C. Full article

Spring droughts, increasingly coinciding with canopy shade, interactively stress the growth of urban tree species and are poorly understood in Beijing. Three-year-old saplings of Pinus tabuliformis and Robinia pseudoacacia were subjected to comparative analysis under four drought–shade sequences, with a full-light, well-watered treatment serving as the control. During two periods encompassing the drought to wilting point and subsequent rewatering, we assessed leaf morphology, water status, photosynthetic gas exchange, and chlorophyll fluorescence. Both species exhibited losses in leaf water and carbon assimilation under drought, yet their adaptive strategies substantially differed. P. tabuliformis conserved water through the stable leaf anatomy and conservative stomatal control. In particular, P. tabuliformis under full-light and drought conditions decreased their specific leaf area (SLA) by 23%, as well as showing reductions in stomatal conductance (G_s) and transpiration rate (T_r) along with the drought duration (p < 0.01). As the duration of post-drought rewatering increased, the reductions in the net photosynthetic rates (P_n) of P. tabulaeformis showed that the shade condition intensified its photosynthetic limitation and slowed recovery after drought. Under low-light drought, R. pseudoacacia exhibited a 52% increase in SLA and a 77% decline in G_s; the latter was markedly smaller than the reduction observed under full-light drought. After rewatering, G_s displayed an overcompensation response. The rise in specific leaf area and the greater flexibility of stomatal regulation partly offset the adverse effects of drought. Nevertheless, post-drought P_n recovered to only 40%, significantly lower than the 61% recovery under full-light drought. Moreover, the negative correlation between SLA and P_n became significantly stronger, indicating that the “after-effects” of shade–drought hindered photosynthetic recovery once the stress was relieved. Drought duration eroded the phenotypic performance in both species, while the light environment during drought and subsequent rehydration determined the time trajectory and completeness of recovery. These results validate a trade-off between shade mitigation and drought legacy, and guide species selection: plant shade-tolerant R. pseudoacacia in light-limited urban pockets and reserve sun-dependent P. tabuliformis for open, high-light sites to enhance drought resilience of Beijing’s urban forests. Full article

To investigate the failure mechanisms of surrounding rock in deep mine tunnels and its spatio-temporal evolution patterns, a true triaxial disturbance unloading rock testing system, the acoustic emission (AE) system, and the miniature camera monitoring system were employed to conduct true triaxial graded loading tests on sandstone containing circular holes at burial depths of 800 m, 1000 m, 1200 m, 1400 m, and 1600 m. The study investigated the patterns of mechanical properties and failure characteristics of porous sandstone at different burial depths. The results showed that the peak strength of the specimens increased quadratically with increasing burial depth; the failure process of porous sandstone could be divided into four stages: the calm period, the particle ejection period, the stable failure period, and the complete collapse period; as burial depth increases, the failure mode transitions from a composite tensile–shear crack type to a shear crack-dominated type, with the ratio of shear cracks to tensile cracks exhibiting quadratic growth and reduction, respectively; the particle ejection stage is characterised by low-frequency, low-amplitude signals, corresponding to the microcrack initiation stage, while the stable failure stage exhibits a sharp increase in low-frequency, high-amplitude signals, reflecting macrocrack propagation characteristics, with the spatial evolution of their locations ultimately forming a penetrating oblique shear failure zone; and peak stress analysis indicates that as burial depth increases, peak stress during the particle ejection phase first increases and then decreases, while peak stress during the stable failure phase first decreases and then stabilises. The duration of the pre-instability calm phase shows a significant negative correlation with burial depth. The research findings provide a theoretical basis for controlling tunnel rock mass stability and disaster warning. Full article