Search Results (1,617)

Plant volatiles are critical mediators of insect–plant interactions, guiding natural enemies to specific habitats and prey. The flower bug, Orius maxidentex Ghauri (Hemiptera: Anthocoridae), is a generalist predator that exhibits a specialized ecological association with the weed Celosia argentea L. (Caryophyllales: Amaranthaceae), utilizing the plant as a primary floral niche in Hainan Island. In this study, the attractiveness of C. argentea floral volatiles to O. maxidentex was confirmed using a Y-tube olfactometer. Solid-phase microextraction (SPME) combined with gas chromatography–mass spectrometry (GC-MS) was utilized to identify six compounds in the floral volatiles: 1,3-diethenylbenzene, trans-cinnamaldehyde, β-bisabolene, methyl salicylate, 3-ethylbenzaldehyde, and nonanal. Electroantennogram (EAG) assays revealed that O. maxidentex antennae showed significant physiological responses to these compounds, and the EAG relative values were positively correlated with concentration gradients. Furthermore, O. maxidentex exhibited significant orientation responses to 1,3-diethenylbenzene, trans-cinnamaldehyde, β-bisabolene, and methyl salicylate, whereas no behavioral response was observed for 3-ethylbenzaldehyde or nonanal. Further tests revealed that β-bisabolene elicited the highest attractiveness, comparable to a synthetic blend formulated to mimic the natural release ratio of the active semiochemicals. These findings reveal the hidden chemical cues mediating the interaction between a predator and its preferred habitat. Understanding this mechanism not only helps explain insect adaptation but also offers new strategies for using these plant volatiles to influence the behavior of this specific predator, potentially enhancing its targeted recruitment in agroecosystems. Full article

Colorectal cancer (CRC) shows consistent sex-related differences in incidence, anatomic distribution, molecular subtype, immune context, and clinical outcome. However, these differences are often discussed through broad parallel themes such as hormones, genetics, or the microbiome, rather than through the biological settings in which sex meaningfully modifies tumor behavior. This review argues that sex is most informative in CRC when treated as a contextual modifier whose relevance emerges only after integrating tumor sidedness, mismatch repair status, oncogenic background, immune ecology, and age at onset. The clearest signals arise from interaction-based contexts, particularly when sex is interpreted together with tumor sidedness and dMMR/MSI-H or BRAF-linked disease states. Current evidence indicates that women are enriched for proximal or right-sided, microsatellite instability-high, mismatch repair-deficient, CpG island methylator phenotype-high, and BRAF-associated CRC, whereas men more often present with distal disease and a higher overall burden. Mechanistic studies further show that sex-related differences extend beyond hormone exposure to include KRAS–STAT4–KDM5D signaling, site-specific immune-checkpoint programs, metabolic phenotypes, epigenetic biomarker variation, and microbiota–hormone crosstalk. These effects are most evident in defined clinical niches, particularly right-sided CRC, mismatch repair-deficient disease, BRAF-mutated metastatic CRC, and early-onset CRC. A sex-aware, subtype-aware, and location-aware framework therefore offers a more clinically useful interpretation of CRC heterogeneity than descriptive male-versus-female comparisons alone. Full article

Antibiotic-associated diarrhea (AAD) is primarily driven by disruption of the gut microbiota accompanied by intestinal mucosal injury. Although multiherb formulations are widely used in East Asian medicine, their collective ecological effects and integrated microbiome–host mechanisms have not been systematically synthesized. This systematic review included 17 preclinical studies that investigated multiherbal formulations in AAD models. Given the substantial heterogeneity in the formulation composition, experimental design, and analytical platforms, a descriptive synthesis was performed. The included formulations were categorized into four clusters based on their shared herbal composition: Qiwei Baizhu San (QWBZP), Lizhong Tang (LZT), Gegen Qinlian Tang (GQT), and other supportive multiherbal formulations. The cluster-based synthesis revealed distinct convergent therapeutic strategies. The QWBZP and LZT clusters primarily supported the restoration of host metabolic and digestive functions, whereas the GQT cluster exhibited potent pathogen control effects with the suppression of opportunistic taxa. Across all clusters, a convergent microbiome–host response emerged, characterized by enrichment of commensal bacteria (e.g., Lactobacillus), upregulation of tight junction proteins (e.g., ZO-1, occludin), and attenuation of pro-inflammatory mediators (e.g., TNF-α, myeloperoxidase). Multiherb formulations in AAD models not only act as microbial modulators but also function as host-directed modulators that stabilize the intestinal homeostatic niche. Botanical interventions may facilitate endogenous microbiome recovery by reinforcing mucosal integrity and reducing environmental resistance. This ecological framework provides a rationale for future translational studies evaluating integrated herbal–probiotic strategies and precise microbiome management for patients with AAD, while further clinical validation is warranted. Full article

The neotropical genus Inga (Fabaceae) is a fast-growing tree component of tropical forests which plays crucial ecological and functional roles. However, its diversity patterns and the specific environmental drivers that structure its distribution in Andean landscapes remain insufficiently documented. This study aimed to quantify the diversity and distribution of Inga species in the province of Imbabura (4785 km²), northern Ecuador, while evaluating the influence of key environmental determinants. By integrating 52 field records along 321 km of exploration and 22 herbarium records (QCNE, MO, AAU, F, HUTN), the study analyzes the role of topographic variables (12.5 m resolution) and climate data (1 km² resolution). Seventeen species were recorded, almost tripling previous regional findings. The results demonstrate that species richness and occurrence are strongly structured by altitude, temperature, and soil properties as primary environmental drivers. Ten species showed narrow altitudinal ranges and limited thermal tolerance (<2 °C), indicating high habitat specialization, while I. densiflora and I. insignis exhibited broader niches. Edaphically, most species were associated with sandy loam soils, particularly Mollisols and Inceptisols developed from volcanic material. These findings indicate that climatic gradients and edaphic conditions act as the main environmental filters shaping Inga assemblages in heterogeneous montane landscapes. The observed high level of specialization suggests significant vulnerability to land-use change and highlights the need for habitat-specific conservation strategies in Andean forests. Full article

Ticks harbor complex microbial communities composed of symbionts, commensals, and tick-borne pathogens (TBPs). Together, these microorganisms form the tick holobiont. Within this system, the tick’s physiological architecture structures microbial communities by distributing microorganisms across distinct tissues. This compartmentalization creates spatially distinct ecological niches, which in turn shape how microbial communities assemble and interact. In this review, we integrate ecological theory with current knowledge of tick microbiome research to examine how pathogen–pathogen and pathogen–microbiome interactions emerge within these tissue-structured microbial communities. We first outline how baseline ecological filters, including tick species, developmental stage, tissue identity, vertical transmission, and environmental context, shape the microbiome configuration through community assembly processes. We then examined how TBPs, as high-impact colonizers, can further modify microbial networks by altering host-mediated selective pressures, influencing interaction topology, and reshaping community stability. Based on these observations, we propose a dual selective pressure framework in which (i) baseline ecological structuring processes and (ii) pathogen-associated selective pressures interact to determine the microbial network configuration and functional outcomes within the tick holobiont. These interacting forces may drive shifts in diversity, modularity, keystone taxa emergence, and network resilience, ultimately influencing vector competence. This review frames the microbial communities within the tick holobiont as spatially structured ecological systems shaped by multilevel selective pressures. This conceptual foundation provides a coherent framework for understanding microbial interactions in arthropod vectors and highlights avenues for mechanistic research and microbiome-based strategies to mitigate tick-borne diseases. Full article

Against the backdrop of global coral reef degradation, benthic resource structure is shifting from coral dominance to turf algae and detritus-dominated epilithic algal matrix (EAM). As a typical detritivorous reef fish, Ctenochaetus striatus (Quoy & Gaimard, 1825) plays an important ecological role in regulating the functioning of degraded coral reef ecosystems. Using stable isotope analysis (δ¹³C and δ¹⁵N), this study systematically compared the trophic niche characteristics of different size classes of C. striatus across four reef habitats in the Xisha Islands, South China Sea, representing a gradient of disturbance (Qilianyu Island > Lingyang Reef > North Reef > Langhua Reef), in order to elucidate habitat-specific ontogenetic shifts and their adaptive features. The results showed that C. striatus from Qilianyu Island and Lingyang Reef exhibited overall higher δ¹⁵N values, suggesting an overall pattern consistent with stronger nitrogen enrichment at the more disturbed reefs, whereas individuals from Langhua Reef had significantly lower δ¹³C values, indicating a stronger reliance on offshore-derived carbon pathways. Across size classes, the trophic niche area (SEAc) and intraspecific trophic heterogeneity, measured as mean nearest neighbor distance and standard deviation of nearest neighbor distance, of populations from Qilianyu Island, Lingyang Reef, and North Reef generally decreased with increasing body size, revealing a pattern of trophic convergence toward core resources. In contrast, the Langhua Reef population exhibited a distinct expansion–contraction pattern, suggesting flexible resource use across developmental stages under conditions of low human disturbance and high resource heterogeneity. Although smaller size classes generally showed high probabilities of niche overlap among reefs, overlap declined markedly in the largest size class, with most values falling below 50%, indicating that resource assimilation strategies increasingly reflected reef-specific resource backgrounds. These findings demonstrate that ontogenetic trophic niche shifts in C. striatus are not fixed, but are highly dependent on local resource context and habitat conditions. In degraded reefs with simplified resource structure, individuals tend to converge on core resource spectra to maintain survival, whereas in healthier reefs with greater habitat heterogeneity, they tend to show greater variation in major food sources and resource use. This study provides a theoretical basis for coral reef ecological restoration. Full article

Using high-resolution field data from the Yunnan Provincial Grassland Pest Survey and an optimized MaxEnt model, we compared the climate-driven habitat dynamics of two invasive Asteraceae weeds (Chromolaena odorata, Ageratina adenophora) and a native weed (Cirsium japonicum). We assessed whether invasive and native weeds differ in environmental responses, future range dynamics, and management strategies, and three novel patterns were revealed. First, the invasive Chromolaena odorata exhibits a sustained positive response to mean annual temperature (contribution 67.6%), while the native Cirsium japonicum shows a strictly unimodal response with a narrow optimum (0–10 °C, contribution 46.4%) and high-temperature sensitivity, projecting over 50% habitat loss by the 2050s under high emissions. Second, the invasive Ageratina adenophora displays a southern contraction versus northern expansion pattern under high emissions (current highly suitable area ~9.12 × 10⁴ km²), suggesting that extreme warming may enable it to breach high-altitude barriers. Third, all three species show unimodal responses to human disturbance with species-specific optima. Overall, the invasive species, leveraging broad ecological amplitudes and strong adaptability, are poised for continued expansion of their potential suitable habitat, while the native species, constrained by a narrow niche and limited dispersal capacity, faces systemic habitat loss. These findings provide a mechanistic basis for differentiating management strategies between invasive and native problematic weeds in Yunnan grasslands. Full article

To date, the problem of mastitis in cattle remains relevant for both the industrial sector and scientific research. Despite numerous active investigations, the causes of this disease have not been fully established. It is postulated that several factors may be involved, such as bacterial pathogens, animal husbandry practices, and weather and climatic conditions. In this study, we selected cows from farms in Yakutia to investigate microbial isolates present in the milk of cows affected by mastitis and treated with antibiotics. Five identified Staphylococcus aureus isolates were investigated using whole-genome sequencing (Illumina sequencing and nanopore sequencing), followed by analysis of virulence factors in the genomes and cultural properties of the isolates. The profile of S. aureus virulence genes (exotoxins, cytotoxins, superantigen-like proteins, adhesins) was identified via WGS. Hemolysin gene (hla) was detected in all isolates. An investigation of the cultural properties of the isolates, specifically through hemolysis of rabbit erythrocytes and Western blot analysis of the culture liquid of S. aureus, revealed different expression levels of alpha-hemolysin among the strains. One isolate (17-21) exhibited the highest secretion level of about 320 ± 37 ng, both in the hemolysis test and immunoblotting assay. An investigation of the isolates’ antibiotic resistance showed that all isolates exhibited multidrug resistance, as confirmed by the presence of antibiotic resistance genes in these isolates. One isolate (7-7) exhibited the broadest range of phenotypic resistance and was resistant to all tested antibiotics (except clindamycin). Phylogenetic analysis suggested that the evolution of these isolates occurred independently in their respective ecological niches, although their transfer from cattle to humans, and vice versa, is possible. Isolates 7-7, 18-22, 33-40, and 35-42 are most typical to Yakutian cattle, while isolate 17-21 might have been introduced from a different region. To the best of our knowledge, this is the first in-depth study into a range of S. aureus isolates associated with mastitis infection in Yakutian cattle. Full article

This study, conducted in the Cheile Nerei–Beușnița National Park (southwestern Romania), tested the hypothesis that the life-form structure of woody plants reflects the main ecological gradients of moisture, temperature, and soil reaction in a temperate protected ecosystem characterised by sub-Mediterranean influences and a predominantly calcareous substrate. The analysis focused on the woody flora of the area, comprising 64 species belonging to 22 families, and included the assessment of life-form structure, phytogeographical spectrum, and ecological preferences based on Ellenberg indicator values. Life forms were classified according to Raunkiaer’s system, identifying megaphanerophytes, mesophanerophytes, and nanophanerophytes. The woody flora was dominated by nanophanerophytes, followed by megaphanerophytes and mesophanerophytes, indicating a complex vertical structure. The phytogeographical spectrum showed a predominance of European elements, alongside Eurasian and sub-Mediterranean components. Ecological analysis revealed a dominance of mesophilous and mesothermal species, consistent with mesic and temperate environmental conditions. Soil reaction preferences were mainly basiphilous and neutrophilous, reflecting the calcareous substrate, with vertical differentiation of ecological niches between tree and shrub layers. The high proportion of native species (>90%) and the limited presence of alien taxa indicate a high level of ecological integrity and resistance to biological invasions. Overall, the results demonstrate that the structure of woody plant life forms and their ecological preferences accurately reflect the main ecological gradients of the ecosystem. The combined use of life-form spectra and ecological indicator values provides a useful framework for assessing ecosystem structure, stability, and conservation status in temperate protected areas. Full article

The Escherichia genus includes both commensal and pathogenic species and is characterized by its diversity and adaptability to the mammalian gut and other environments. Among these species, E. coli has facilitated many scientific advances as a model organism. Recently, a new member of the Escherichia genus, Escherichia marmotae, has been described as a phylogenetically distinct clade that shows the greatest genetic divergence from E. coli. This review explores E. marmotae, its cryptic evolution, distinct characteristics, and ecological niches. E. marmotae has recently gained scientific prominence due to its association with animal feces, environmental occurrence, human clinical samples, and emerging as a potential pathogen. While its pathogenicity remains understudied, growing evidence from clinical, environmental, and animal sources suggests the need for heightened surveillance. This review highlights current knowledge gaps, underscores the need for improved diagnostic tools, and proposes future research directions to elucidate the clinical and ecological implications of this emerging pathogen. Full article

Hydrological fluctuations drive community dynamics in floodplain lakes, yet their integration into water resource management remains limited. Here, we integrated environmental DNA (eDNA) metabarcoding with hydroacoustic surveys to investigate vertebrate community assembly in China’s largest freshwater lake (Poyang Lake) during the winter dry season. We detected 65 vertebrate species, with Cypriniformes dominating. Beta-diversity partitioning revealed that turnover dominated taxonomic and functional dissimilarity, while phylogenetic beta diversity was characterized by nestedness, which is consistent with environmental filtering. Functional richness declined with water depth, coinciding with hydroacoustic vertical size stratification, indicating niche partitioning along depth gradients. Null model analysis showed stochastic processes (ecological drift) dominated regional assembly (72.97%), whereas joint species distribution modeling attributed explained variation to environmental factors (28.9%, notably water depth) and species associations (29.7%) at local scales. This hierarchical framework, regional stochasticity shaping the species pool and local deterministic filtering structuring communities, reframes environmental flow conceptualization: effective management must preserve the full spectrum of hydrological variability and maintain bathymetrically diverse habitats that support functional niche differentiation. The integrated eDNA-hydroacoustic approach offers a non-invasive, high-resolution toolkit for biological assessment within regulatory water quality frameworks. Full article

To improve the degradation rate of sulfamethoxazole (SMX) under hypoxic conditions in paddy fields during the flooding period, a laboratory microcosm experiment was established in this study to investigate the regulatory effect of straw return on the hypoxic degradation of SMX and its underlying microbial mechanisms. The results demonstrated that straw addition significantly promoted the hypoxic degradation of SMX. By the 10th day of the experiment, the residual SMX content in the straw-amended group was only 11.3% of that in the non-straw control group, and the average degradation rate throughout the experimental period was increased by 85.7%. Straw provided complex carbon sources, including organic carbon (e.g., starch, sugars, and humus) and cellulose, which not only reshaped the microbial community structure and created new ecological niches but also increased the microbial network density under SMX stress from 0.010 to 0.024. Acinetobacter, Bacillus, and Pseudomonas, which possess both straw decomposition and SMX degradation capabilities, sequentially became the dominant bacterial taxa. These dominant taxa can adapt to hypoxic fermentation in the flooded environment and co-metabolize SMX by activating the metabolic pathways of aromatic compounds and sugars. This study is expected to open up a novel approach for the remediation of SMX-contaminated flooded paddy fields and provide valuable innovative insights for technological breakthroughs and practical applications in related fields. Full article

Earthworms are ecosystem engineers that are sensitive to land-use intensification and edaphic conditions, yet their ecology remains poorly understood in transformed semi-arid landscapes. We hypothesized that, in recently colonized agroecosystems, land-use intensity and physicochemical soil conditions jointly filter the earthworm assembly. In the recently irrigated Lower Valley of the Negro River, Patagonia, Argentina, we sampled earthworms and soils across five land uses—riparian reference sites, fruit orchards, pastures, cereal crops, and horticulture plots—in landscapes dominated by Natrargid Ustolls and Fluventic Haplocambids. We found five species, all of which were exotic Lumbricidae, including the first Argentine record for Murchieona minuscula, indicating a recent colonization following human-mediated niche construction that created an ecological island. The earthworm abundance and biomass were highest in permanent and semi-permanent uses and were driven primarily by soil moisture, pH, and particulate organic matter. Crucially, our results reveal that land-use intensity filters communities by restricting the initial colonization rather than through local extinctions. These findings confirm that soil properties mediate the impact of land use on earthworm assemblages. The inclusion of pastures and fruit orchards in the rotations favors the earthworm populations that, despite low diversity, enhance soil functioning and contribute to agricultural sustainability in semi-arid irrigated agroecosystems. Full article

Background/Objectives: Staphylococcus aureus (S. aureus) intramammary infection remains a major global dairy problem due to its contagious nature, its ability to persist and colonize teat/skin and mucosal niches, and the often-limited bacteriological cure achieved with antimicrobial therapy. Beyond udder health, it is relevant to public health because it can enter raw milk chains and serve as a reservoir for antimicrobial resistance determinants that may circulate between dairy animals and humans. Methods: We assessed S. aureus’ ecology in raw ewe milk from 75 sheep farms in Epirus (Greece) by sampling clinically healthy controls (group A) and clinical mastitis cases pre-treatment (group B), followed by resampling at the first post-withdrawal milking after penicillin/streptomycin treatment (group C1—therapeutic protocol 1), oxytetracycline treatment (group C2—therapeutic protocol 2), or enrofloxacin treatment (group C3—therapeutic protocol 3). Results: S. aureus detection was high and comparable across groups (A 23.0%, B 22.0–30.0%, C 20.0–22.0%), and paired analyses showed no significant pre–post shifts in detection/burden within therapeutic protocols (all p > 0.05). Nevertheless, persistence remained evident. The chromosomal gene mecA was detected in S. aureus strains in all groups, ranging from 13.6% in controls to 54.5% post-withdrawal in group C1, and was also present in the pre-treatment group. In paired sampling animals, mecA was mostly stable, with rare emergence or loss. Across antibiotic classes, within-animal resistance transitions were generally uncommon and non-significant (p > 0.05); β-lactam resistance was fully stable (p = 1.00). Descriptively, resistance to protein synthesis inhibitors tended to decline after therapy in protocol 1 and protocol 3, while protocol 3 showed post-treatment gains in fluoroquinolone resistance. By contrast, virulence-associated phenotype traits shifted after therapy: enterotoxigenicity increased post-withdrawal (especially in the C3 group), Staphylococcal Enterotoxin A (SEA) and Staphylococcal Enterotoxin B (SEB) appeared only post-therapy, Staphylococcal Enterotoxin D (SED) increased significantly in paired isolates (p = 0.002), and strong biofilm adherence increased (in C3, p = 1.5 × 10⁻⁵). Conclusions: The detection of S. aureus after therapy suggests that one possibility is that antimicrobial exposure may select for, or otherwise reshape, the residual intramammary population, rather than reliably eliminating it—an outcome that remains clinically relevant for udder health. Moreover, the persistence of mecA/methicillin-resistant Staphylococcus aureus (MRSA)-compatible profiles indicates that milk released to the food chain after withdrawal compliance may still harbor S. aureus with enhanced preservation capacity and significant food safety relevance. Full article

The daily activity patterns of wild animal species are driven by environmental conditions and plant productivity although the degree of dependence varies according to their ecological niche. Bear ecology is intrinsically linked to seasonal vegetative availability. As omnivores with high metabolic demands, these species rely heavily on botanical resources including fruits, seeds, and roots. Consequently, differences in primary productivity across the landscape influence how individuals distribute their circadian activity patterns. The Enhanced Vegetation Index (EVI) is a tool that quantifies the quality and vigor of vegetation. Relating the EVI to activity patterns allows us to understand how vegetation dynamics and conditions influence the use of time at different times of the day. This study analyzes the daily activity pattern of the American black bear (Ursus americanus) in the El Cielo Biosphere Reserve (ECBR) using camera traps and its association with spatial variations in the Enhanced Vegetation Index (EVI). The results show that the daily activity pattern of the American black bear in the ECBR exhibits a diurnal–crepuscular tendency. In areas with high primary productivity and higher temperatures, activity occurs before sunrise and at sunset, with low activity during the rest of the day. In contrast, in areas with less vegetation and lower temperatures, activity occurs throughout the day. This suggests that, in the ECBR, the activity pattern of black bears could be modulated by temperature variations related to changes in vegetation productivity. Full article